Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02860 and MMJJ_RS02865 are separated by 103 nucleotides MMJJ_RS02865 and MMJJ_RS02870 are separated by 115 nucleotides
MMJJ_RS02860: MMJJ_RS02860 - site-specific integrase, at 539,832 to 540,380
_RS02860
MMJJ_RS02865: MMJJ_RS02865 - amino acid ABC transporter substrate-binding protein, at 540,484 to 541,230
_RS02865
MMJJ_RS02870: MMJJ_RS02870 - hypothetical protein, at 541,346 to 541,867
_RS02870
Position (kb) 539
540
541 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 538.900 kb on + strand at 538.900 kb on + strand at 538.943 kb on - strand at 538.950 kb on + strand at 538.953 kb on + strand at 538.957 kb on - strand at 538.957 kb on - strand at 538.965 kb on - strand at 538.975 kb on - strand at 538.976 kb on + strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.994 kb on - strand at 538.995 kb on + strand at 538.995 kb on + strand at 538.995 kb on + strand at 538.998 kb on - strand at 538.999 kb on + strand at 539.001 kb on - strand at 539.001 kb on - strand at 539.025 kb on + strand at 539.028 kb on - strand at 539.028 kb on - strand at 539.055 kb on - strand at 539.059 kb on - strand at 539.059 kb on - strand at 539.059 kb on - strand at 539.059 kb on - strand at 539.059 kb on - strand at 539.060 kb on + strand at 539.060 kb on + strand at 539.060 kb on + strand at 539.108 kb on - strand at 539.116 kb on - strand at 539.116 kb on - strand at 539.118 kb on - strand at 539.118 kb on - strand at 539.118 kb on - strand at 539.118 kb on - strand at 539.119 kb on + strand at 539.119 kb on + strand at 539.139 kb on + strand at 539.140 kb on - strand at 539.141 kb on + strand at 539.210 kb on - strand at 539.210 kb on - strand at 539.211 kb on + strand at 539.211 kb on + strand at 539.221 kb on - strand at 539.226 kb on + strand at 539.226 kb on + strand at 539.226 kb on + strand at 539.229 kb on - strand at 539.230 kb on + strand at 539.230 kb on + strand at 539.230 kb on + strand at 539.233 kb on - strand at 539.234 kb on + strand at 539.243 kb on - strand at 539.244 kb on + strand at 539.244 kb on + strand at 539.244 kb on + strand at 539.244 kb on + strand at 539.244 kb on + strand at 539.254 kb on - strand at 539.264 kb on - strand at 539.265 kb on + strand at 539.265 kb on + strand at 539.265 kb on + strand at 539.265 kb on + strand at 539.265 kb on + strand at 539.266 kb on - strand at 539.266 kb on - strand at 539.266 kb on - strand at 539.267 kb on + strand at 539.267 kb on + strand at 539.267 kb on + strand at 539.268 kb on - strand at 539.268 kb on - strand at 539.268 kb on - strand at 539.268 kb on - strand at 539.269 kb on + strand at 539.269 kb on + strand at 539.269 kb on + strand at 539.269 kb on + strand at 539.273 kb on - strand at 539.274 kb on + strand at 539.278 kb on - strand at 539.323 kb on - strand at 539.337 kb on - strand at 539.337 kb on - strand at 539.367 kb on - strand at 539.725 kb on - strand at 539.725 kb on - strand at 539.726 kb on + strand at 539.726 kb on + strand at 539.727 kb on - strand at 539.727 kb on - strand at 539.820 kb on - strand at 539.821 kb on + strand at 539.845 kb on + strand at 539.876 kb on + strand at 539.897 kb on + strand, within MMJJ_RS02860 at 540.224 kb on - strand, within MMJJ_RS02860 at 540.295 kb on - strand, within MMJJ_RS02860 at 540.412 kb on - strand at 540.426 kb on + strand at 540.450 kb on + strand at 540.452 kb on - strand at 540.455 kb on + strand at 540.464 kb on - strand at 540.477 kb on - strand at 540.478 kb on + strand at 540.524 kb on - strand at 540.540 kb on - strand at 540.540 kb on - strand at 540.540 kb on - strand at 540.541 kb on + strand at 540.567 kb on - strand, within MMJJ_RS02865 at 540.712 kb on - strand, within MMJJ_RS02865 at 540.713 kb on + strand, within MMJJ_RS02865 at 540.814 kb on + strand, within MMJJ_RS02865 at 540.814 kb on + strand, within MMJJ_RS02865 at 540.923 kb on - strand, within MMJJ_RS02865 at 540.971 kb on - strand, within MMJJ_RS02865 at 540.972 kb on + strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.001 kb on + strand, within MMJJ_RS02865 at 541.069 kb on - strand, within MMJJ_RS02865 at 541.089 kb on + strand, within MMJJ_RS02865 at 541.089 kb on + strand, within MMJJ_RS02865 at 541.096 kb on + strand, within MMJJ_RS02865 at 541.096 kb on + strand, within MMJJ_RS02865 at 541.128 kb on + strand, within MMJJ_RS02865 at 541.149 kb on + strand, within MMJJ_RS02865 at 541.196 kb on + strand at 541.228 kb on - strand at 541.260 kb on - strand at 541.261 kb on + strand at 541.264 kb on + strand at 541.264 kb on + strand at 541.264 kb on + strand at 541.278 kb on + strand at 541.278 kb on + strand at 541.358 kb on - strand at 541.367 kb on - strand at 541.368 kb on + strand at 541.368 kb on + strand at 541.372 kb on - strand at 541.373 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 538,900 + -2.8 538,900 + -1.3 538,943 - -0.9 538,950 + +1.4 538,953 + -3.1 538,957 - -4.3 538,957 - -0.2 538,965 - +1.4 538,975 - -1.8 538,976 + +1.0 538,992 - -2.1 538,992 - -1.4 538,992 - -1.4 538,992 - +0.9 538,992 - -0.4 538,992 - -0.2 538,992 - -0.2 538,992 - +0.1 538,993 + -0.9 538,993 + -0.7 538,993 + -0.2 538,993 + -0.1 538,993 + -0.2 538,993 + +1.3 538,993 + +2.3 538,994 - -0.9 538,995 + -2.1 538,995 + -0.7 538,995 + +0.6 538,998 - -0.9 538,999 + +0.6 539,001 - +1.3 539,001 - +1.5 539,025 + +0.8 539,028 - -0.9 539,028 - -0.7 539,055 - -0.8 539,059 - +0.9 539,059 - -0.6 539,059 - -0.1 539,059 - +2.0 539,059 - +2.2 539,060 + -1.5 539,060 + +1.7 539,060 + -1.2 539,108 - +0.1 539,116 - +0.4 539,116 - +0.8 539,118 - -1.2 539,118 - +0.4 539,118 - -1.3 539,118 - +0.7 539,119 + +0.2 539,119 + +0.6 539,139 + +0.0 539,140 - +0.1 539,141 + -0.8 539,210 - +0.7 539,210 - -1.5 539,211 + -0.5 539,211 + +0.4 539,221 - +0.9 539,226 + +0.4 539,226 + +0.5 539,226 + +0.6 539,229 - +2.7 539,230 + +0.6 539,230 + +0.4 539,230 + -1.1 539,233 - -1.6 539,234 + +0.2 539,243 - -0.4 539,244 + +0.4 539,244 + +1.9 539,244 + -0.6 539,244 + -0.9 539,244 + -1.3 539,254 - -1.4 539,264 - +0.3 539,265 + -0.5 539,265 + -3.4 539,265 + +1.7 539,265 + -0.7 539,265 + -0.5 539,266 - +0.4 539,266 - -0.9 539,266 - -0.8 539,267 + -0.9 539,267 + +0.8 539,267 + +0.7 539,268 - +0.1 539,268 - -0.8 539,268 - -0.2 539,268 - -1.2 539,269 + -1.2 539,269 + -0.1 539,269 + +1.1 539,269 + +0.1 539,273 - +0.2 539,274 + -1.4 539,278 - -1.4 539,323 - -0.4 539,337 - +0.5 539,337 - +1.3 539,367 - -0.0 539,725 - +0.0 539,725 - +2.4 539,726 + -0.5 539,726 + +1.0 539,727 - +1.1 539,727 - +1.0 539,820 - +0.4 539,821 + -1.9 539,845 + -2.0 539,876 + +0.2 539,897 + MMJJ_RS02860 0.12 +0.3 540,224 - MMJJ_RS02860 0.71 -0.2 540,295 - MMJJ_RS02860 0.84 +1.3 540,412 - -0.0 540,426 + -1.2 540,450 + -0.7 540,452 - +1.3 540,455 + +0.3 540,464 - +0.8 540,477 - -0.1 540,478 + -1.0 540,524 - +0.2 540,540 - -2.6 540,540 - -0.2 540,540 - +0.7 540,541 + +0.2 540,567 - MMJJ_RS02865 0.11 +0.3 540,712 - MMJJ_RS02865 0.31 +0.5 540,713 + MMJJ_RS02865 0.31 +0.3 540,814 + MMJJ_RS02865 0.44 -0.6 540,814 + MMJJ_RS02865 0.44 +1.1 540,923 - MMJJ_RS02865 0.59 +0.6 540,971 - MMJJ_RS02865 0.65 -0.7 540,972 + MMJJ_RS02865 0.65 +1.9 541,000 - MMJJ_RS02865 0.69 -0.3 541,000 - MMJJ_RS02865 0.69 -1.8 541,000 - MMJJ_RS02865 0.69 -2.1 541,000 - MMJJ_RS02865 0.69 +0.4 541,001 + MMJJ_RS02865 0.69 -0.3 541,069 - MMJJ_RS02865 0.78 -0.9 541,089 + MMJJ_RS02865 0.81 +0.2 541,089 + MMJJ_RS02865 0.81 +0.3 541,096 + MMJJ_RS02865 0.82 -1.0 541,096 + MMJJ_RS02865 0.82 -0.9 541,128 + MMJJ_RS02865 0.86 +1.3 541,149 + MMJJ_RS02865 0.89 -1.0 541,196 + +0.1 541,228 - -0.5 541,260 - -0.3 541,261 + +2.0 541,264 + +2.1 541,264 + -2.6 541,264 + +0.8 541,278 + -1.1 541,278 + +0.0 541,358 - -0.8 541,367 - -0.6 541,368 + -0.2 541,368 + -0.7 541,372 - -1.2 541,373 + +0.4
Or see this region's nucleotide sequence