Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01060

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01050 and MMJJ_RS01055 are separated by 10 nucleotidesMMJJ_RS01055 and MMJJ_RS01060 are separated by 7 nucleotidesMMJJ_RS01060 and MMJJ_RS01065 are separated by 154 nucleotidesMMJJ_RS01065 and MMJJ_RS01070 overlap by 8 nucleotides MMJJ_RS01050: MMJJ_RS01050 - COG1361 S-layer family protein, at 205,028 to 206,287 _RS01050 MMJJ_RS01055: MMJJ_RS01055 - hypothetical protein, at 206,298 to 206,780 _RS01055 MMJJ_RS01060: MMJJ_RS01060 - ABC transporter ATP-binding protein, at 206,788 to 207,492 _RS01060 MMJJ_RS01065: MMJJ_RS01065 - DUF1700 domain-containing protein, at 207,647 to 208,231 _RS01065 MMJJ_RS01070: MMJJ_RS01070 - PadR family transcriptional regulator, at 208,224 to 208,547 _RS01070 Position (kb) 206 207 208Strain fitness (log2 ratio) -2 -1 0 1 2at 205.797 kb on + strand, within MMJJ_RS01050at 205.847 kb on - strand, within MMJJ_RS01050at 205.867 kb on + strand, within MMJJ_RS01050at 205.904 kb on - strand, within MMJJ_RS01050at 205.943 kb on - strand, within MMJJ_RS01050at 205.954 kb on - strand, within MMJJ_RS01050at 205.955 kb on + strand, within MMJJ_RS01050at 205.966 kb on - strand, within MMJJ_RS01050at 205.971 kb on + strand, within MMJJ_RS01050at 206.001 kb on + strand, within MMJJ_RS01050at 206.072 kb on - strand, within MMJJ_RS01050at 206.072 kb on - strand, within MMJJ_RS01050at 206.077 kb on + strand, within MMJJ_RS01050at 206.120 kb on + strand, within MMJJ_RS01050at 206.120 kb on + strandat 206.166 kb on + strandat 206.174 kb on + strandat 206.258 kb on + strandat 206.306 kb on - strandat 206.371 kb on - strand, within MMJJ_RS01055at 206.473 kb on - strand, within MMJJ_RS01055at 206.542 kb on + strand, within MMJJ_RS01055at 206.587 kb on + strandat 206.667 kb on - strand, within MMJJ_RS01055at 206.726 kb on + strand, within MMJJ_RS01055at 206.843 kb on + strandat 206.964 kb on - strand, within MMJJ_RS01060at 207.138 kb on - strand, within MMJJ_RS01060at 207.207 kb on - strand, within MMJJ_RS01060at 207.209 kb on - strand, within MMJJ_RS01060at 207.209 kb on - strand, within MMJJ_RS01060at 207.210 kb on + strand, within MMJJ_RS01060at 207.221 kb on + strand, within MMJJ_RS01060at 207.222 kb on - strand, within MMJJ_RS01060at 207.258 kb on - strand, within MMJJ_RS01060at 207.280 kb on + strand, within MMJJ_RS01060at 207.295 kb on - strand, within MMJJ_RS01060at 207.403 kb on + strand, within MMJJ_RS01060at 207.406 kb on - strand, within MMJJ_RS01060at 207.472 kb on + strandat 207.478 kb on - strandat 207.481 kb on - strandat 207.481 kb on - strandat 207.481 kb on - strandat 207.481 kb on - strandat 207.515 kb on - strandat 207.519 kb on + strandat 207.546 kb on + strandat 207.634 kb on - strandat 207.733 kb on - strand, within MMJJ_RS01065at 207.733 kb on - strand, within MMJJ_RS01065at 207.734 kb on + strand, within MMJJ_RS01065at 207.801 kb on + strand, within MMJJ_RS01065at 207.819 kb on + strand, within MMJJ_RS01065at 207.850 kb on + strand, within MMJJ_RS01065at 207.939 kb on + strand, within MMJJ_RS01065at 207.973 kb on - strand, within MMJJ_RS01065at 207.978 kb on + strand, within MMJJ_RS01065at 207.980 kb on - strand, within MMJJ_RS01065at 207.980 kb on - strand, within MMJJ_RS01065at 207.981 kb on + strand, within MMJJ_RS01065at 207.981 kb on + strand, within MMJJ_RS01065at 207.981 kb on + strand, within MMJJ_RS01065at 207.983 kb on + strand, within MMJJ_RS01065at 207.992 kb on - strand, within MMJJ_RS01065at 207.993 kb on + strand, within MMJJ_RS01065at 207.993 kb on + strand, within MMJJ_RS01065at 207.993 kb on + strand, within MMJJ_RS01065at 208.062 kb on + strand, within MMJJ_RS01065at 208.091 kb on + strand, within MMJJ_RS01065at 208.154 kb on - strand, within MMJJ_RS01065at 208.154 kb on - strand, within MMJJ_RS01065at 208.211 kb on - strandat 208.331 kb on - strand, within MMJJ_RS01070at 208.373 kb on + strand, within MMJJ_RS01070at 208.420 kb on + strand, within MMJJ_RS01070at 208.491 kb on + strand, within MMJJ_RS01070

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
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205,797 + MMJJ_RS01050 0.61 +0.1
205,847 - MMJJ_RS01050 0.65 -1.5
205,867 + MMJJ_RS01050 0.67 -1.4
205,904 - MMJJ_RS01050 0.70 +0.1
205,943 - MMJJ_RS01050 0.73 +0.8
205,954 - MMJJ_RS01050 0.73 -0.5
205,955 + MMJJ_RS01050 0.74 +2.0
205,966 - MMJJ_RS01050 0.74 -0.8
205,971 + MMJJ_RS01050 0.75 -0.1
206,001 + MMJJ_RS01050 0.77 -0.8
206,072 - MMJJ_RS01050 0.83 +2.1
206,072 - MMJJ_RS01050 0.83 -0.0
206,077 + MMJJ_RS01050 0.83 -1.0
206,120 + MMJJ_RS01050 0.87 -0.6
206,120 + +1.8
206,166 + -1.8
206,174 + +0.3
206,258 + -0.4
206,306 - -1.7
206,371 - MMJJ_RS01055 0.15 +0.4
206,473 - MMJJ_RS01055 0.36 +0.2
206,542 + MMJJ_RS01055 0.51 -0.9
206,587 + -0.2
206,667 - MMJJ_RS01055 0.76 -0.9
206,726 + MMJJ_RS01055 0.89 -0.2
206,843 + +0.6
206,964 - MMJJ_RS01060 0.25 -0.5
207,138 - MMJJ_RS01060 0.50 +2.0
207,207 - MMJJ_RS01060 0.59 -0.9
207,209 - MMJJ_RS01060 0.60 +0.8
207,209 - MMJJ_RS01060 0.60 +0.5
207,210 + MMJJ_RS01060 0.60 -1.2
207,221 + MMJJ_RS01060 0.61 -1.3
207,222 - MMJJ_RS01060 0.62 +1.0
207,258 - MMJJ_RS01060 0.67 +0.8
207,280 + MMJJ_RS01060 0.70 +0.8
207,295 - MMJJ_RS01060 0.72 +0.0
207,403 + MMJJ_RS01060 0.87 +1.9
207,406 - MMJJ_RS01060 0.88 +0.5
207,472 + -0.8
207,478 - +0.4
207,481 - +2.5
207,481 - +0.3
207,481 - -1.6
207,481 - +0.1
207,515 - -0.3
207,519 + +0.4
207,546 + -1.8
207,634 - -1.2
207,733 - MMJJ_RS01065 0.15 -1.2
207,733 - MMJJ_RS01065 0.15 -0.8
207,734 + MMJJ_RS01065 0.15 +0.7
207,801 + MMJJ_RS01065 0.26 -1.8
207,819 + MMJJ_RS01065 0.29 -0.8
207,850 + MMJJ_RS01065 0.35 -0.1
207,939 + MMJJ_RS01065 0.50 -0.6
207,973 - MMJJ_RS01065 0.56 +1.0
207,978 + MMJJ_RS01065 0.57 -1.5
207,980 - MMJJ_RS01065 0.57 +0.3
207,980 - MMJJ_RS01065 0.57 +0.1
207,981 + MMJJ_RS01065 0.57 -0.7
207,981 + MMJJ_RS01065 0.57 +1.4
207,981 + MMJJ_RS01065 0.57 +2.3
207,983 + MMJJ_RS01065 0.57 +0.4
207,992 - MMJJ_RS01065 0.59 +0.5
207,993 + MMJJ_RS01065 0.59 +0.4
207,993 + MMJJ_RS01065 0.59 -1.0
207,993 + MMJJ_RS01065 0.59 +0.5
208,062 + MMJJ_RS01065 0.71 +0.5
208,091 + MMJJ_RS01065 0.76 +0.7
208,154 - MMJJ_RS01065 0.87 +1.0
208,154 - MMJJ_RS01065 0.87 +0.4
208,211 - -0.6
208,331 - MMJJ_RS01070 0.33 -0.5
208,373 + MMJJ_RS01070 0.46 -1.6
208,420 + MMJJ_RS01070 0.60 +1.0
208,491 + MMJJ_RS01070 0.82 -1.3

Or see this region's nucleotide sequence