Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35710 and RR42_RS35715 are separated by 56 nucleotides RR42_RS35715 and RR42_RS35720 are separated by 14 nucleotides RR42_RS35720 and RR42_RS35725 are separated by 7 nucleotides
RR42_RS35710: RR42_RS35710 - flagellar hook protein FlgE, at 3,462,244 to 3,463,485
_RS35710
RR42_RS35715: RR42_RS35715 - flagellar basal body rod modification protein FlgD, at 3,463,542 to 3,464,213
_RS35715
RR42_RS35720: RR42_RS35720 - flagellar basal body rod protein FlgC, at 3,464,228 to 3,464,635
_RS35720
RR42_RS35725: RR42_RS35725 - flagellar biosynthesis protein FlgB, at 3,464,643 to 3,465,053
_RS35725
Position (kb)
3463
3464
3465 Strain fitness (log2 ratio)
-1
0
1
2 at 3462.599 kb on + strand, within RR42_RS35710 at 3462.920 kb on + strand, within RR42_RS35710 at 3462.920 kb on + strand, within RR42_RS35710 at 3462.920 kb on + strand, within RR42_RS35710 at 3462.941 kb on + strand, within RR42_RS35710 at 3462.942 kb on - strand, within RR42_RS35710 at 3462.942 kb on - strand, within RR42_RS35710 at 3463.202 kb on + strand, within RR42_RS35710 at 3463.202 kb on + strand, within RR42_RS35710 at 3463.496 kb on + strand at 3463.501 kb on - strand at 3463.544 kb on - strand at 3463.702 kb on + strand, within RR42_RS35715 at 3463.977 kb on + strand, within RR42_RS35715 at 3464.298 kb on + strand, within RR42_RS35720 at 3464.298 kb on + strand, within RR42_RS35720 at 3464.679 kb on - strand at 3464.942 kb on - strand, within RR42_RS35725 at 3464.951 kb on + strand, within RR42_RS35725 at 3464.951 kb on + strand, within RR42_RS35725 at 3464.951 kb on + strand, within RR42_RS35725 at 3465.197 kb on + strand at 3465.197 kb on + strand at 3465.203 kb on - strand at 3465.203 kb on - strand at 3465.203 kb on - strand at 3465.203 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 3,462,599 + RR42_RS35710 0.29 +0.1 3,462,920 + RR42_RS35710 0.54 -1.0 3,462,920 + RR42_RS35710 0.54 +0.6 3,462,920 + RR42_RS35710 0.54 -1.4 3,462,941 + RR42_RS35710 0.56 -1.3 3,462,942 - RR42_RS35710 0.56 -0.1 3,462,942 - RR42_RS35710 0.56 +0.4 3,463,202 + RR42_RS35710 0.77 +0.0 3,463,202 + RR42_RS35710 0.77 +0.6 3,463,496 + +1.6 3,463,501 - -0.0 3,463,544 - -0.2 3,463,702 + RR42_RS35715 0.24 -0.2 3,463,977 + RR42_RS35715 0.65 +1.6 3,464,298 + RR42_RS35720 0.17 +0.1 3,464,298 + RR42_RS35720 0.17 +0.1 3,464,679 - +0.2 3,464,942 - RR42_RS35725 0.73 +0.7 3,464,951 + RR42_RS35725 0.75 -0.7 3,464,951 + RR42_RS35725 0.75 -0.1 3,464,951 + RR42_RS35725 0.75 +1.9 3,465,197 + +1.3 3,465,197 + -1.4 3,465,203 - +0.2 3,465,203 - +0.1 3,465,203 - +0.6 3,465,203 - +0.9
Or see this region's nucleotide sequence