Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34515 and RR42_RS34520 are separated by 190 nucleotides RR42_RS34520 and RR42_RS34525 are separated by 74 nucleotides RR42_RS34525 and RR42_RS34530 overlap by 4 nucleotides
RR42_RS34515: RR42_RS34515 - acyltransferase, at 3,175,716 to 3,176,858
_RS34515
RR42_RS34520: RR42_RS34520 - transposase, at 3,177,049 to 3,177,483
_RS34520
RR42_RS34525: RR42_RS34525 - type VI secretion protein VasK, at 3,177,558 to 3,181,133
_RS34525
RR42_RS34530: RR42_RS34530 - hypothetical protein, at 3,181,130 to 3,182,371
_RS34530
Position (kb)
3177
3178
3179
3180
3181
3182 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3176.598 kb on + strand, within RR42_RS34515 at 3176.605 kb on - strand, within RR42_RS34515 at 3176.613 kb on + strand, within RR42_RS34515 at 3176.613 kb on + strand, within RR42_RS34515 at 3176.614 kb on - strand, within RR42_RS34515 at 3176.614 kb on - strand, within RR42_RS34515 at 3176.914 kb on + strand at 3176.914 kb on + strand at 3177.009 kb on + strand at 3177.009 kb on + strand at 3177.010 kb on - strand at 3177.010 kb on - strand at 3177.013 kb on + strand at 3177.013 kb on + strand at 3177.014 kb on - strand at 3177.014 kb on - strand at 3177.015 kb on + strand at 3177.015 kb on + strand at 3177.015 kb on + strand at 3177.016 kb on - strand at 3177.016 kb on - strand at 3177.016 kb on - strand at 3177.428 kb on + strand, within RR42_RS34520 at 3177.472 kb on - strand at 3177.472 kb on - strand at 3178.925 kb on - strand, within RR42_RS34525 at 3179.212 kb on + strand, within RR42_RS34525 at 3179.212 kb on + strand, within RR42_RS34525 at 3179.495 kb on - strand, within RR42_RS34525 at 3179.495 kb on - strand, within RR42_RS34525 at 3180.810 kb on + strand at 3180.889 kb on + strand at 3180.890 kb on - strand at 3180.890 kb on - strand at 3180.950 kb on - strand at 3181.083 kb on + strand at 3181.091 kb on + strand at 3181.099 kb on + strand at 3181.099 kb on + strand at 3181.100 kb on - strand at 3181.100 kb on - strand at 3181.100 kb on - strand at 3181.100 kb on - strand at 3181.108 kb on - strand at 3181.124 kb on - strand at 3181.583 kb on + strand, within RR42_RS34530 at 3181.584 kb on - strand, within RR42_RS34530 at 3181.584 kb on - strand, within RR42_RS34530 at 3181.694 kb on + strand, within RR42_RS34530 at 3181.694 kb on + strand, within RR42_RS34530 at 3181.695 kb on - strand, within RR42_RS34530 at 3181.695 kb on - strand, within RR42_RS34530 at 3181.695 kb on - strand, within RR42_RS34530 at 3181.757 kb on + strand, within RR42_RS34530 at 3181.758 kb on - strand, within RR42_RS34530 at 3181.763 kb on + strand, within RR42_RS34530 at 3181.764 kb on - strand, within RR42_RS34530 at 3181.764 kb on - strand, within RR42_RS34530 at 3181.847 kb on - strand, within RR42_RS34530
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 3,176,598 + RR42_RS34515 0.77 -0.1 3,176,605 - RR42_RS34515 0.78 +0.8 3,176,613 + RR42_RS34515 0.78 +0.5 3,176,613 + RR42_RS34515 0.78 +1.0 3,176,614 - RR42_RS34515 0.79 -0.6 3,176,614 - RR42_RS34515 0.79 +1.1 3,176,914 + -1.1 3,176,914 + -1.6 3,177,009 + +1.0 3,177,009 + -0.2 3,177,010 - -0.3 3,177,010 - -0.1 3,177,013 + +0.4 3,177,013 + +0.3 3,177,014 - +0.5 3,177,014 - -0.3 3,177,015 + -0.1 3,177,015 + +0.4 3,177,015 + -0.1 3,177,016 - -0.3 3,177,016 - +1.8 3,177,016 - -1.2 3,177,428 + RR42_RS34520 0.87 -0.3 3,177,472 - -0.6 3,177,472 - -0.3 3,178,925 - RR42_RS34525 0.38 +0.8 3,179,212 + RR42_RS34525 0.46 +0.7 3,179,212 + RR42_RS34525 0.46 +0.7 3,179,495 - RR42_RS34525 0.54 +0.1 3,179,495 - RR42_RS34525 0.54 +1.0 3,180,810 + -0.1 3,180,889 + +0.2 3,180,890 - +0.2 3,180,890 - +0.0 3,180,950 - +1.6 3,181,083 + -0.1 3,181,091 + -0.5 3,181,099 + +1.2 3,181,099 + -0.8 3,181,100 - +0.3 3,181,100 - +0.0 3,181,100 - +0.0 3,181,100 - -0.3 3,181,108 - +0.1 3,181,124 - -0.0 3,181,583 + RR42_RS34530 0.36 +0.5 3,181,584 - RR42_RS34530 0.37 +1.9 3,181,584 - RR42_RS34530 0.37 -2.0 3,181,694 + RR42_RS34530 0.45 -0.9 3,181,694 + RR42_RS34530 0.45 +1.7 3,181,695 - RR42_RS34530 0.45 +0.6 3,181,695 - RR42_RS34530 0.45 -1.2 3,181,695 - RR42_RS34530 0.45 +0.1 3,181,757 + RR42_RS34530 0.50 -0.9 3,181,758 - RR42_RS34530 0.51 -0.2 3,181,763 + RR42_RS34530 0.51 +0.2 3,181,764 - RR42_RS34530 0.51 -0.5 3,181,764 - RR42_RS34530 0.51 +1.0 3,181,847 - RR42_RS34530 0.58 -0.3
Or see this region's nucleotide sequence