Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31860 and RR42_RS31865 are separated by 149 nucleotides RR42_RS31865 and RR42_RS31870 are separated by 213 nucleotides RR42_RS31870 and RR42_RS31875 are separated by 142 nucleotides
RR42_RS31860: RR42_RS31860 - 4-hyroxy-2-oxovalerate aldolase, at 2,524,642 to 2,525,664
_RS31860
RR42_RS31865: RR42_RS31865 - prevent-host-death protein, at 2,525,814 to 2,526,047
_RS31865
RR42_RS31870: RR42_RS31870 - 3-ketosteroid-9-alpha-hydroxylase, at 2,526,261 to 2,527,337
_RS31870
RR42_RS31875: RR42_RS31875 - hypothetical protein, at 2,527,480 to 2,528,670
_RS31875
Position (kb)
2526
2527
2528 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2525.500 kb on + strand, within RR42_RS31860 at 2525.500 kb on + strand, within RR42_RS31860 at 2525.542 kb on + strand, within RR42_RS31860 at 2525.543 kb on - strand, within RR42_RS31860 at 2525.725 kb on + strand at 2525.781 kb on + strand at 2525.781 kb on + strand at 2525.781 kb on + strand at 2525.782 kb on - strand at 2525.797 kb on + strand at 2525.797 kb on + strand at 2525.798 kb on - strand at 2525.798 kb on - strand at 2525.957 kb on + strand, within RR42_RS31865 at 2525.958 kb on - strand, within RR42_RS31865 at 2526.185 kb on + strand at 2526.368 kb on - strand at 2526.397 kb on + strand, within RR42_RS31870 at 2526.500 kb on - strand, within RR42_RS31870 at 2526.500 kb on - strand, within RR42_RS31870 at 2526.781 kb on + strand, within RR42_RS31870 at 2526.831 kb on - strand, within RR42_RS31870 at 2527.146 kb on - strand, within RR42_RS31870 at 2527.175 kb on + strand, within RR42_RS31870 at 2527.322 kb on - strand at 2527.322 kb on - strand at 2527.350 kb on + strand at 2527.356 kb on + strand at 2527.357 kb on - strand at 2527.357 kb on - strand at 2527.357 kb on - strand at 2527.697 kb on + strand, within RR42_RS31875 at 2527.697 kb on + strand, within RR42_RS31875 at 2527.751 kb on + strand, within RR42_RS31875 at 2527.751 kb on + strand, within RR42_RS31875 at 2527.752 kb on - strand, within RR42_RS31875 at 2527.752 kb on - strand, within RR42_RS31875 at 2527.874 kb on + strand, within RR42_RS31875 at 2527.875 kb on - strand, within RR42_RS31875 at 2528.099 kb on + strand, within RR42_RS31875 at 2528.099 kb on + strand, within RR42_RS31875 at 2528.219 kb on + strand, within RR42_RS31875 at 2528.242 kb on - strand, within RR42_RS31875 at 2528.242 kb on - strand, within RR42_RS31875
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 2,525,500 + RR42_RS31860 0.84 +1.3 2,525,500 + RR42_RS31860 0.84 -0.7 2,525,542 + RR42_RS31860 0.88 +0.2 2,525,543 - RR42_RS31860 0.88 -0.5 2,525,725 + +0.2 2,525,781 + -0.4 2,525,781 + +0.4 2,525,781 + -0.9 2,525,782 - -0.2 2,525,797 + +0.2 2,525,797 + -0.1 2,525,798 - -0.7 2,525,798 - -0.6 2,525,957 + RR42_RS31865 0.61 -0.1 2,525,958 - RR42_RS31865 0.62 -0.7 2,526,185 + -0.2 2,526,368 - +0.0 2,526,397 + RR42_RS31870 0.13 +0.8 2,526,500 - RR42_RS31870 0.22 +0.8 2,526,500 - RR42_RS31870 0.22 +0.3 2,526,781 + RR42_RS31870 0.48 +0.5 2,526,831 - RR42_RS31870 0.53 -1.2 2,527,146 - RR42_RS31870 0.82 +0.5 2,527,175 + RR42_RS31870 0.85 +0.3 2,527,322 - +0.4 2,527,322 - -0.1 2,527,350 + -0.6 2,527,356 + -0.5 2,527,357 - +0.2 2,527,357 - +0.0 2,527,357 - +0.6 2,527,697 + RR42_RS31875 0.18 -2.4 2,527,697 + RR42_RS31875 0.18 +0.5 2,527,751 + RR42_RS31875 0.23 -0.3 2,527,751 + RR42_RS31875 0.23 -1.5 2,527,752 - RR42_RS31875 0.23 -0.2 2,527,752 - RR42_RS31875 0.23 -0.1 2,527,874 + RR42_RS31875 0.33 +0.4 2,527,875 - RR42_RS31875 0.33 -0.4 2,528,099 + RR42_RS31875 0.52 -0.7 2,528,099 + RR42_RS31875 0.52 +0.6 2,528,219 + RR42_RS31875 0.62 +2.6 2,528,242 - RR42_RS31875 0.64 +0.2 2,528,242 - RR42_RS31875 0.64 -0.1
Or see this region's nucleotide sequence