Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21995 and RR42_RS22000 are separated by 1 nucleotides RR42_RS22000 and RR42_RS22005 are separated by 201 nucleotides
RR42_RS21995: RR42_RS21995 - glycosyl transferase family 1, at 265,574 to 267,175
_RS21995
RR42_RS22000: RR42_RS22000 - histidine kinase, at 267,177 to 268,547
_RS22000
RR42_RS22005: RR42_RS22005 - transcriptional regulator, at 268,749 to 269,432
_RS22005
Position (kb)
267
268
269 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 266.231 kb on + strand, within RR42_RS21995 at 266.231 kb on + strand, within RR42_RS21995 at 266.232 kb on - strand, within RR42_RS21995 at 266.232 kb on - strand, within RR42_RS21995 at 266.232 kb on - strand, within RR42_RS21995 at 266.232 kb on - strand, within RR42_RS21995 at 266.461 kb on + strand, within RR42_RS21995 at 266.462 kb on - strand, within RR42_RS21995 at 266.582 kb on - strand, within RR42_RS21995 at 266.663 kb on + strand, within RR42_RS21995 at 266.663 kb on + strand, within RR42_RS21995 at 266.806 kb on - strand, within RR42_RS21995 at 267.035 kb on + strand at 267.074 kb on + strand at 267.074 kb on + strand at 267.075 kb on - strand at 267.253 kb on - strand at 267.378 kb on + strand, within RR42_RS22000 at 267.379 kb on - strand, within RR42_RS22000 at 267.438 kb on - strand, within RR42_RS22000 at 267.487 kb on - strand, within RR42_RS22000 at 267.658 kb on - strand, within RR42_RS22000 at 267.693 kb on - strand, within RR42_RS22000 at 267.693 kb on - strand, within RR42_RS22000 at 267.693 kb on - strand, within RR42_RS22000 at 267.730 kb on - strand, within RR42_RS22000 at 267.813 kb on + strand, within RR42_RS22000 at 267.814 kb on - strand, within RR42_RS22000 at 267.814 kb on - strand, within RR42_RS22000 at 267.859 kb on - strand, within RR42_RS22000 at 267.894 kb on + strand, within RR42_RS22000 at 267.894 kb on + strand, within RR42_RS22000 at 267.895 kb on - strand, within RR42_RS22000 at 267.895 kb on - strand, within RR42_RS22000 at 267.895 kb on - strand, within RR42_RS22000 at 267.895 kb on - strand, within RR42_RS22000 at 267.987 kb on + strand, within RR42_RS22000 at 267.987 kb on + strand, within RR42_RS22000 at 267.988 kb on - strand, within RR42_RS22000 at 268.089 kb on + strand, within RR42_RS22000 at 268.089 kb on + strand, within RR42_RS22000 at 268.089 kb on + strand, within RR42_RS22000 at 268.124 kb on + strand, within RR42_RS22000 at 268.133 kb on + strand, within RR42_RS22000 at 268.274 kb on - strand, within RR42_RS22000 at 268.278 kb on + strand, within RR42_RS22000 at 268.279 kb on - strand, within RR42_RS22000 at 268.279 kb on - strand, within RR42_RS22000 at 268.279 kb on - strand, within RR42_RS22000 at 268.335 kb on + strand, within RR42_RS22000 at 268.335 kb on + strand, within RR42_RS22000 at 268.336 kb on - strand, within RR42_RS22000 at 268.336 kb on - strand, within RR42_RS22000 at 268.341 kb on + strand, within RR42_RS22000 at 268.440 kb on + strand at 268.491 kb on + strand at 268.533 kb on + strand at 268.533 kb on + strand at 268.586 kb on - strand at 268.627 kb on + strand at 268.628 kb on - strand at 268.645 kb on + strand at 268.744 kb on + strand at 268.744 kb on + strand at 268.745 kb on - strand at 268.745 kb on - strand at 268.745 kb on - strand at 268.748 kb on + strand at 268.749 kb on - strand at 269.043 kb on + strand, within RR42_RS22005 at 269.044 kb on - strand, within RR42_RS22005 at 269.044 kb on - strand, within RR42_RS22005 at 269.044 kb on - strand, within RR42_RS22005 at 269.087 kb on - strand, within RR42_RS22005 at 269.154 kb on + strand, within RR42_RS22005 at 269.155 kb on - strand, within RR42_RS22005 at 269.155 kb on - strand, within RR42_RS22005 at 269.155 kb on - strand, within RR42_RS22005 at 269.155 kb on - strand, within RR42_RS22005 at 269.333 kb on + strand, within RR42_RS22005 at 269.339 kb on + strand, within RR42_RS22005 at 269.340 kb on - strand, within RR42_RS22005 at 269.340 kb on - strand, within RR42_RS22005 at 269.340 kb on - strand, within RR42_RS22005 at 269.340 kb on - strand, within RR42_RS22005 at 269.404 kb on - strand at 269.490 kb on - strand at 269.517 kb on + strand at 269.518 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 266,231 + RR42_RS21995 0.41 -0.4 266,231 + RR42_RS21995 0.41 -0.2 266,232 - RR42_RS21995 0.41 +0.9 266,232 - RR42_RS21995 0.41 -0.8 266,232 - RR42_RS21995 0.41 -0.3 266,232 - RR42_RS21995 0.41 -0.2 266,461 + RR42_RS21995 0.55 +0.0 266,462 - RR42_RS21995 0.55 +0.7 266,582 - RR42_RS21995 0.63 -0.9 266,663 + RR42_RS21995 0.68 -0.3 266,663 + RR42_RS21995 0.68 -0.4 266,806 - RR42_RS21995 0.77 +0.8 267,035 + -0.3 267,074 + +1.0 267,074 + -0.4 267,075 - -0.5 267,253 - -0.3 267,378 + RR42_RS22000 0.15 -2.7 267,379 - RR42_RS22000 0.15 +0.1 267,438 - RR42_RS22000 0.19 +0.3 267,487 - RR42_RS22000 0.23 +0.6 267,658 - RR42_RS22000 0.35 -0.1 267,693 - RR42_RS22000 0.38 +1.0 267,693 - RR42_RS22000 0.38 +0.1 267,693 - RR42_RS22000 0.38 +0.3 267,730 - RR42_RS22000 0.40 -1.0 267,813 + RR42_RS22000 0.46 +0.2 267,814 - RR42_RS22000 0.46 +0.4 267,814 - RR42_RS22000 0.46 +0.9 267,859 - RR42_RS22000 0.50 +1.9 267,894 + RR42_RS22000 0.52 +0.9 267,894 + RR42_RS22000 0.52 -1.7 267,895 - RR42_RS22000 0.52 -1.0 267,895 - RR42_RS22000 0.52 +0.9 267,895 - RR42_RS22000 0.52 +0.9 267,895 - RR42_RS22000 0.52 +0.5 267,987 + RR42_RS22000 0.59 +1.0 267,987 + RR42_RS22000 0.59 -0.0 267,988 - RR42_RS22000 0.59 +0.9 268,089 + RR42_RS22000 0.67 -0.3 268,089 + RR42_RS22000 0.67 +0.1 268,089 + RR42_RS22000 0.67 -0.5 268,124 + RR42_RS22000 0.69 +0.0 268,133 + RR42_RS22000 0.70 +0.2 268,274 - RR42_RS22000 0.80 +0.5 268,278 + RR42_RS22000 0.80 -0.1 268,279 - RR42_RS22000 0.80 -0.1 268,279 - RR42_RS22000 0.80 -1.3 268,279 - RR42_RS22000 0.80 -0.4 268,335 + RR42_RS22000 0.84 +0.2 268,335 + RR42_RS22000 0.84 +0.3 268,336 - RR42_RS22000 0.85 -0.1 268,336 - RR42_RS22000 0.85 +0.4 268,341 + RR42_RS22000 0.85 +0.5 268,440 + +0.7 268,491 + -0.8 268,533 + +0.4 268,533 + +0.8 268,586 - +0.7 268,627 + -0.2 268,628 - +0.1 268,645 + +0.4 268,744 + -0.7 268,744 + +0.2 268,745 - -0.5 268,745 - -0.1 268,745 - +0.8 268,748 + +0.1 268,749 - +1.4 269,043 + RR42_RS22005 0.43 +0.0 269,044 - RR42_RS22005 0.43 -0.1 269,044 - RR42_RS22005 0.43 +0.2 269,044 - RR42_RS22005 0.43 -0.1 269,087 - RR42_RS22005 0.49 -0.8 269,154 + RR42_RS22005 0.59 +0.0 269,155 - RR42_RS22005 0.59 +0.2 269,155 - RR42_RS22005 0.59 -0.6 269,155 - RR42_RS22005 0.59 -0.4 269,155 - RR42_RS22005 0.59 -1.4 269,333 + RR42_RS22005 0.85 -1.0 269,339 + RR42_RS22005 0.86 +0.2 269,340 - RR42_RS22005 0.86 -1.4 269,340 - RR42_RS22005 0.86 -0.6 269,340 - RR42_RS22005 0.86 +0.3 269,340 - RR42_RS22005 0.86 +0.4 269,404 - -0.2 269,490 - -2.8 269,517 + +0.4 269,518 - -0.7
Or see this region's nucleotide sequence