Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21285 and RR42_RS21290 are separated by 35 nucleotides RR42_RS21290 and RR42_RS21295 overlap by 4 nucleotides RR42_RS21295 and RR42_RS21300 are separated by 138 nucleotides
RR42_RS21285: RR42_RS21285 - L-lactate dehydrogenase, LutB subunit <small>(from data)</small>, at 102,111 to 103,541
_RS21285
RR42_RS21290: RR42_RS21290 - L-lactate dehydrogenase, LutC subunit <small>(from data)</small>, at 103,577 to 104,281
_RS21290
RR42_RS21295: RR42_RS21295 - L-lactate dehydrogenase, LutA subunit <small>(from data)</small>, at 104,278 to 105,066
_RS21295
RR42_RS21300: RR42_RS21300 - GntR family transcriptional regulator, at 105,205 to 105,930
_RS21300
Position (kb) 103
104
105 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 102.637 kb on + strand, within RR42_RS21285 at 102.733 kb on + strand, within RR42_RS21285 at 102.734 kb on - strand, within RR42_RS21285 at 103.013 kb on - strand, within RR42_RS21285 at 103.028 kb on + strand, within RR42_RS21285 at 103.123 kb on + strand, within RR42_RS21285 at 103.124 kb on - strand, within RR42_RS21285 at 103.171 kb on + strand, within RR42_RS21285 at 103.574 kb on + strand at 103.574 kb on + strand at 103.575 kb on - strand at 103.575 kb on - strand at 103.616 kb on - strand at 103.695 kb on - strand, within RR42_RS21290 at 103.695 kb on - strand, within RR42_RS21290 at 103.779 kb on + strand, within RR42_RS21290 at 103.889 kb on - strand, within RR42_RS21290 at 104.175 kb on + strand, within RR42_RS21290 at 104.175 kb on + strand, within RR42_RS21290 at 104.175 kb on + strand, within RR42_RS21290 at 104.176 kb on - strand, within RR42_RS21290 at 104.240 kb on + strand at 104.711 kb on + strand, within RR42_RS21295 at 104.895 kb on + strand, within RR42_RS21295 at 104.896 kb on - strand, within RR42_RS21295 at 105.026 kb on + strand at 105.130 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 102,637 + RR42_RS21285 0.37 -1.5 102,733 + RR42_RS21285 0.43 +0.5 102,734 - RR42_RS21285 0.44 -1.4 103,013 - RR42_RS21285 0.63 -2.4 103,028 + RR42_RS21285 0.64 -1.1 103,123 + RR42_RS21285 0.71 -2.7 103,124 - RR42_RS21285 0.71 -0.9 103,171 + RR42_RS21285 0.74 -1.0 103,574 + -0.4 103,574 + -4.3 103,575 - -3.1 103,575 - -2.4 103,616 - -1.2 103,695 - RR42_RS21290 0.17 -0.8 103,695 - RR42_RS21290 0.17 -0.8 103,779 + RR42_RS21290 0.29 -0.3 103,889 - RR42_RS21290 0.44 +1.1 104,175 + RR42_RS21290 0.85 -0.6 104,175 + RR42_RS21290 0.85 -2.0 104,175 + RR42_RS21290 0.85 -2.5 104,176 - RR42_RS21290 0.85 +0.8 104,240 + +1.9 104,711 + RR42_RS21295 0.55 -1.2 104,895 + RR42_RS21295 0.78 -1.5 104,896 - RR42_RS21295 0.78 -0.8 105,026 + -4.2 105,130 + -3.3
Or see this region's nucleotide sequence