Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14115 and RR42_RS14120 are separated by 323 nucleotides RR42_RS14120 and RR42_RS14125 are separated by 119 nucleotides RR42_RS14125 and RR42_RS14130 are separated by 248 nucleotides RR42_RS14130 and RR42_RS14135 are separated by 51 nucleotides
RR42_RS14115: RR42_RS14115 - phosphate acyltransferase, at 3,036,266 to 3,037,342
_RS14115
RR42_RS14120: RR42_RS14120 - 50S ribosomal protein L32, at 3,037,666 to 3,037,845
_RS14120
RR42_RS14125: RR42_RS14125 - metal-binding protein, at 3,037,965 to 3,038,561
_RS14125
RR42_RS14130: RR42_RS14130 - septum formation inhibitor Maf, at 3,038,810 to 3,039,409
_RS14130
RR42_RS14135: RR42_RS14135 - SAM-dependent methyltransferase, at 3,039,461 to 3,040,192
_RS14135
Position (kb)
3037
3038
3039 Strain fitness (log2 ratio)
-2
-1
0
1 at 3037.499 kb on - strand at 3037.499 kb on - strand at 3037.622 kb on - strand at 3037.622 kb on - strand at 3037.622 kb on - strand at 3038.279 kb on - strand, within RR42_RS14125 at 3038.405 kb on - strand, within RR42_RS14125 at 3038.567 kb on - strand at 3038.622 kb on - strand at 3038.626 kb on - strand at 3038.626 kb on - strand at 3038.626 kb on - strand at 3038.700 kb on - strand at 3038.736 kb on - strand at 3038.805 kb on + strand at 3038.805 kb on + strand at 3038.806 kb on - strand at 3038.806 kb on - strand at 3038.806 kb on - strand at 3038.806 kb on - strand at 3038.806 kb on - strand at 3038.806 kb on - strand at 3038.809 kb on + strand at 3038.809 kb on + strand at 3038.809 kb on + strand at 3038.809 kb on + strand at 3038.810 kb on - strand at 3038.810 kb on - strand at 3038.810 kb on - strand at 3039.236 kb on + strand, within RR42_RS14130 at 3039.236 kb on + strand, within RR42_RS14130 at 3039.236 kb on + strand, within RR42_RS14130 at 3039.236 kb on + strand, within RR42_RS14130 at 3039.237 kb on - strand, within RR42_RS14130 at 3039.237 kb on - strand, within RR42_RS14130 at 3039.237 kb on - strand, within RR42_RS14130 at 3039.237 kb on - strand, within RR42_RS14130 at 3039.237 kb on - strand, within RR42_RS14130 at 3039.257 kb on + strand, within RR42_RS14130 at 3039.257 kb on + strand, within RR42_RS14130 at 3039.257 kb on + strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.258 kb on - strand, within RR42_RS14130 at 3039.313 kb on + strand, within RR42_RS14130 at 3039.313 kb on + strand, within RR42_RS14130 at 3039.342 kb on + strand, within RR42_RS14130 at 3039.343 kb on - strand, within RR42_RS14130 at 3039.370 kb on + strand at 3039.370 kb on + strand at 3039.370 kb on + strand at 3039.370 kb on + strand at 3039.370 kb on + strand at 3039.371 kb on - strand at 3039.371 kb on - strand at 3039.477 kb on - strand at 3039.477 kb on - strand at 3039.477 kb on - strand at 3039.477 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 3,037,499 - -1.6 3,037,499 - -0.2 3,037,622 - +1.1 3,037,622 - -1.1 3,037,622 - +0.4 3,038,279 - RR42_RS14125 0.53 +1.0 3,038,405 - RR42_RS14125 0.74 +0.7 3,038,567 - -0.8 3,038,622 - +1.4 3,038,626 - +0.2 3,038,626 - -0.2 3,038,626 - +0.1 3,038,700 - -0.8 3,038,736 - +1.4 3,038,805 + +0.1 3,038,805 + +0.4 3,038,806 - -1.0 3,038,806 - -0.4 3,038,806 - -0.0 3,038,806 - +0.2 3,038,806 - -0.6 3,038,806 - +0.9 3,038,809 + +0.2 3,038,809 + -0.1 3,038,809 + -0.2 3,038,809 + -0.2 3,038,810 - +0.3 3,038,810 - +0.5 3,038,810 - -1.0 3,039,236 + RR42_RS14130 0.71 -1.3 3,039,236 + RR42_RS14130 0.71 -0.0 3,039,236 + RR42_RS14130 0.71 +0.2 3,039,236 + RR42_RS14130 0.71 -0.1 3,039,237 - RR42_RS14130 0.71 +0.1 3,039,237 - RR42_RS14130 0.71 +0.0 3,039,237 - RR42_RS14130 0.71 -0.9 3,039,237 - RR42_RS14130 0.71 +1.0 3,039,237 - RR42_RS14130 0.71 -1.9 3,039,257 + RR42_RS14130 0.74 -0.4 3,039,257 + RR42_RS14130 0.74 +0.3 3,039,257 + RR42_RS14130 0.74 +0.2 3,039,258 - RR42_RS14130 0.75 +0.0 3,039,258 - RR42_RS14130 0.75 +0.5 3,039,258 - RR42_RS14130 0.75 +0.7 3,039,258 - RR42_RS14130 0.75 -0.2 3,039,258 - RR42_RS14130 0.75 -0.3 3,039,258 - RR42_RS14130 0.75 -0.6 3,039,258 - RR42_RS14130 0.75 -0.1 3,039,313 + RR42_RS14130 0.84 +0.2 3,039,313 + RR42_RS14130 0.84 +0.1 3,039,342 + RR42_RS14130 0.89 +0.1 3,039,343 - RR42_RS14130 0.89 -0.3 3,039,370 + -0.2 3,039,370 + +0.0 3,039,370 + +0.9 3,039,370 + -0.1 3,039,370 + +0.1 3,039,371 - -0.5 3,039,371 - -1.1 3,039,477 - -0.4 3,039,477 - +0.1 3,039,477 - +0.7 3,039,477 - +0.3
Or see this region's nucleotide sequence