Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08190 and RR42_RS08195 are separated by 238 nucleotides RR42_RS08195 and RR42_RS08200 overlap by 4 nucleotides RR42_RS08200 and RR42_RS08205 overlap by 4 nucleotides RR42_RS08205 and RR42_RS08210 are separated by 347 nucleotides RR42_RS08210 and RR42_RS08215 are separated by 101 nucleotides
RR42_RS08190: RR42_RS08190 - hypothetical protein, at 1,741,454 to 1,741,774
_RS08190
RR42_RS08195: RR42_RS08195 - hypothetical protein, at 1,742,013 to 1,742,531
_RS08195
RR42_RS08200: RR42_RS08200 - hypothetical protein, at 1,742,528 to 1,742,743
_RS08200
RR42_RS08205: RR42_RS08205 - hypothetical protein, at 1,742,740 to 1,743,105
_RS08205
RR42_RS08210: RR42_RS08210 - hypothetical protein, at 1,743,453 to 1,743,794
_RS08210
RR42_RS08215: RR42_RS08215 - DNA-binding protein, at 1,743,896 to 1,744,501
_RS08215
Position (kb) 1742
1743
1744 Strain fitness (log2 ratio) -1
0
1 at 1741.755 kb on - strand at 1741.958 kb on + strand at 1743.187 kb on - strand at 1743.412 kb on - strand at 1743.754 kb on - strand, within RR42_RS08210 at 1743.812 kb on - strand at 1743.917 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 1,741,755 - +0.4 1,741,958 + +0.7 1,743,187 - -0.1 1,743,412 - -1.2 1,743,754 - RR42_RS08210 0.88 -1.2 1,743,812 - -1.1 1,743,917 + -0.2
Or see this region's nucleotide sequence