Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05120 and RR42_RS05125 are separated by 189 nucleotides RR42_RS05125 and RR42_RS05130 overlap by 4 nucleotides RR42_RS05130 and RR42_RS05135 overlap by 4 nucleotides RR42_RS05135 and RR42_RS05140 are separated by 46 nucleotides
RR42_RS05120: RR42_RS05120 - ABC transporter substrate-binding protein, at 1,130,304 to 1,131,284
_RS05120
RR42_RS05125: RR42_RS05125 - malonic semialdehyde reductase, at 1,131,474 to 1,132,058
_RS05125
RR42_RS05130: RR42_RS05130 - 2-aminomuconate deaminase, at 1,132,055 to 1,132,468
_RS05130
RR42_RS05135: RR42_RS05135 - dihydrodipicolinate synthetase, at 1,132,465 to 1,133,379
_RS05135
RR42_RS05140: RR42_RS05140 - lipase, at 1,133,426 to 1,134,364
_RS05140
Position (kb) 1132
1133 Strain fitness (log2 ratio) -1
0
1
2 at 1131.674 kb on - strand, within RR42_RS05125 at 1131.746 kb on - strand, within RR42_RS05125 at 1131.959 kb on - strand, within RR42_RS05125 at 1131.959 kb on - strand, within RR42_RS05125 at 1132.128 kb on - strand, within RR42_RS05130 at 1132.218 kb on + strand, within RR42_RS05130 at 1132.218 kb on + strand, within RR42_RS05130 at 1132.218 kb on + strand, within RR42_RS05130 at 1132.219 kb on - strand, within RR42_RS05130 at 1132.703 kb on + strand, within RR42_RS05135 at 1132.704 kb on - strand, within RR42_RS05135 at 1132.716 kb on + strand, within RR42_RS05135 at 1132.961 kb on + strand, within RR42_RS05135 at 1133.015 kb on + strand, within RR42_RS05135 at 1133.016 kb on - strand, within RR42_RS05135 at 1133.428 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 1,131,674 - RR42_RS05125 0.34 +1.3 1,131,746 - RR42_RS05125 0.46 +0.6 1,131,959 - RR42_RS05125 0.83 -0.3 1,131,959 - RR42_RS05125 0.83 -0.4 1,132,128 - RR42_RS05130 0.18 +2.5 1,132,218 + RR42_RS05130 0.39 +0.6 1,132,218 + RR42_RS05130 0.39 -0.8 1,132,218 + RR42_RS05130 0.39 +1.2 1,132,219 - RR42_RS05130 0.40 +0.9 1,132,703 + RR42_RS05135 0.26 +1.3 1,132,704 - RR42_RS05135 0.26 -0.5 1,132,716 + RR42_RS05135 0.27 -0.9 1,132,961 + RR42_RS05135 0.54 -0.9 1,133,015 + RR42_RS05135 0.60 +0.7 1,133,016 - RR42_RS05135 0.60 +0.3 1,133,428 - +0.2
Or see this region's nucleotide sequence