Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02070 and RR42_RS02075 are separated by 35 nucleotides RR42_RS02075 and RR42_RS02080 are separated by 384 nucleotides RR42_RS02080 and RR42_RS02085 are separated by 49 nucleotides
RR42_RS02070: RR42_RS02070 - HPr kinase, at 460,970 to 461,941
_RS02070
RR42_RS02075: RR42_RS02075 - PTS sugar transporter subunit IIA, at 461,977 to 462,432
_RS02075
RR42_RS02080: RR42_RS02080 - ribosome hibernation promoting factor, at 462,817 to 463,167
_RS02080
RR42_RS02085: RR42_RS02085 - RNA polymerase subunit sigma-54, at 463,217 to 464,692
_RS02085
Position (kb) 462
463
464 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 462.454 kb on - strand at 462.454 kb on - strand at 462.568 kb on - strand at 462.568 kb on - strand at 462.569 kb on + strand at 462.569 kb on + strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.603 kb on + strand at 462.604 kb on - strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.661 kb on + strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.673 kb on + strand at 462.673 kb on + strand at 462.674 kb on - strand at 462.674 kb on - strand at 462.674 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.685 kb on + strand at 462.686 kb on - strand at 462.686 kb on - strand at 462.686 kb on - strand at 462.686 kb on - strand at 462.722 kb on - strand at 462.722 kb on - strand at 462.722 kb on - strand at 462.797 kb on - strand at 462.849 kb on - strand at 462.849 kb on - strand at 462.942 kb on - strand, within RR42_RS02080 at 462.942 kb on - strand, within RR42_RS02080 at 462.991 kb on + strand, within RR42_RS02080 at 462.991 kb on + strand, within RR42_RS02080 at 462.992 kb on - strand, within RR42_RS02080 at 463.032 kb on + strand, within RR42_RS02080 at 463.032 kb on + strand, within RR42_RS02080 at 463.033 kb on - strand, within RR42_RS02080 at 463.033 kb on - strand, within RR42_RS02080 at 463.033 kb on - strand, within RR42_RS02080 at 463.057 kb on + strand, within RR42_RS02080 at 463.057 kb on + strand, within RR42_RS02080 at 463.057 kb on + strand, within RR42_RS02080 at 463.089 kb on + strand, within RR42_RS02080 at 463.112 kb on + strand, within RR42_RS02080 at 463.112 kb on + strand, within RR42_RS02080 at 463.113 kb on - strand, within RR42_RS02080 at 463.113 kb on - strand, within RR42_RS02080 at 463.168 kb on - strand at 463.218 kb on + strand at 463.218 kb on + strand at 463.218 kb on + strand at 463.219 kb on - strand at 463.229 kb on - strand at 463.238 kb on + strand at 463.428 kb on - strand, within RR42_RS02085 at 463.482 kb on + strand, within RR42_RS02085 at 463.483 kb on - strand, within RR42_RS02085 at 463.486 kb on + strand, within RR42_RS02085 at 463.487 kb on - strand, within RR42_RS02085 at 463.531 kb on + strand, within RR42_RS02085 at 463.588 kb on - strand, within RR42_RS02085 at 463.628 kb on + strand, within RR42_RS02085 at 463.629 kb on - strand, within RR42_RS02085 at 463.651 kb on + strand, within RR42_RS02085 at 463.652 kb on - strand, within RR42_RS02085 at 463.740 kb on + strand, within RR42_RS02085 at 463.741 kb on - strand, within RR42_RS02085 at 463.741 kb on - strand, within RR42_RS02085 at 463.857 kb on + strand, within RR42_RS02085
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 462,454 - +2.9 462,454 - +0.2 462,568 - -0.4 462,568 - -0.7 462,569 + -1.0 462,569 + +1.6 462,570 - +0.2 462,570 - +0.6 462,570 - +1.2 462,570 - +0.8 462,570 - +0.1 462,570 - +0.3 462,570 - +0.5 462,570 - +0.5 462,570 - -1.0 462,603 + -1.5 462,604 - +0.5 462,657 + -1.0 462,657 + +1.2 462,657 + -2.0 462,657 + -0.1 462,657 + -0.4 462,658 - +0.8 462,658 - -0.1 462,658 - +1.2 462,658 - -0.4 462,658 - +0.8 462,661 + -1.0 462,662 - +0.5 462,662 - -1.2 462,662 - +0.3 462,662 - +2.2 462,662 - +0.3 462,673 + +0.4 462,673 + +0.9 462,674 - +0.6 462,674 - +1.0 462,674 - +0.1 462,682 - -0.7 462,682 - +0.9 462,682 - +0.2 462,682 - +1.2 462,682 - +0.7 462,685 + -2.0 462,686 - +0.8 462,686 - -0.0 462,686 - +0.8 462,686 - +0.7 462,722 - +0.9 462,722 - -0.2 462,722 - +0.1 462,797 - +0.1 462,849 - -0.5 462,849 - +0.5 462,942 - RR42_RS02080 0.36 -0.2 462,942 - RR42_RS02080 0.36 +0.3 462,991 + RR42_RS02080 0.50 -0.8 462,991 + RR42_RS02080 0.50 +0.8 462,992 - RR42_RS02080 0.50 +1.2 463,032 + RR42_RS02080 0.61 -0.3 463,032 + RR42_RS02080 0.61 -0.4 463,033 - RR42_RS02080 0.62 +0.4 463,033 - RR42_RS02080 0.62 -1.5 463,033 - RR42_RS02080 0.62 +0.4 463,057 + RR42_RS02080 0.68 -0.2 463,057 + RR42_RS02080 0.68 +0.3 463,057 + RR42_RS02080 0.68 +1.2 463,089 + RR42_RS02080 0.77 -0.5 463,112 + RR42_RS02080 0.84 -0.7 463,112 + RR42_RS02080 0.84 +0.4 463,113 - RR42_RS02080 0.84 +0.3 463,113 - RR42_RS02080 0.84 +0.7 463,168 - -0.2 463,218 + -1.5 463,218 + +0.0 463,218 + +0.4 463,219 - +0.5 463,229 - -1.7 463,238 + +1.2 463,428 - RR42_RS02085 0.14 -0.4 463,482 + RR42_RS02085 0.18 -1.7 463,483 - RR42_RS02085 0.18 -2.8 463,486 + RR42_RS02085 0.18 +1.2 463,487 - RR42_RS02085 0.18 -2.5 463,531 + RR42_RS02085 0.21 -2.3 463,588 - RR42_RS02085 0.25 -3.5 463,628 + RR42_RS02085 0.28 -2.0 463,629 - RR42_RS02085 0.28 -2.8 463,651 + RR42_RS02085 0.29 -2.8 463,652 - RR42_RS02085 0.29 -1.0 463,740 + RR42_RS02085 0.35 -3.2 463,741 - RR42_RS02085 0.36 -1.8 463,741 - RR42_RS02085 0.36 -0.8 463,857 + RR42_RS02085 0.43 -3.0
Or see this region's nucleotide sequence