Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01735 and RR42_RS01740 are separated by 291 nucleotides RR42_RS01740 and RR42_RS01745 are separated by 129 nucleotides RR42_RS01745 and RR42_RS01750 are separated by 378 nucleotides
RR42_RS01735: RR42_RS01735 - lysine transporter LysE, at 395,868 to 396,521
_RS01735
RR42_RS01740: RR42_RS01740 - ATP-dependent protease, at 396,813 to 398,357
_RS01740
RR42_RS01745: RR42_RS01745 - phosphoheptose isomerase, at 398,487 to 398,750
_RS01745
RR42_RS01750: RR42_RS01750 - hypothetical protein, at 399,129 to 399,938
_RS01750
Position (kb)
396
397
398
399 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 395.861 kb on + strand at 395.862 kb on - strand at 395.862 kb on - strand at 395.862 kb on - strand at 395.892 kb on + strand at 395.967 kb on + strand, within RR42_RS01735 at 395.968 kb on - strand, within RR42_RS01735 at 396.111 kb on + strand, within RR42_RS01735 at 396.112 kb on - strand, within RR42_RS01735 at 396.120 kb on + strand, within RR42_RS01735 at 396.120 kb on + strand, within RR42_RS01735 at 396.120 kb on + strand, within RR42_RS01735 at 396.120 kb on + strand, within RR42_RS01735 at 396.120 kb on + strand, within RR42_RS01735 at 396.120 kb on + strand, within RR42_RS01735 at 396.120 kb on + strand, within RR42_RS01735 at 396.120 kb on + strand, within RR42_RS01735 at 396.121 kb on - strand, within RR42_RS01735 at 396.121 kb on - strand, within RR42_RS01735 at 396.121 kb on - strand, within RR42_RS01735 at 396.121 kb on - strand, within RR42_RS01735 at 396.121 kb on - strand, within RR42_RS01735 at 396.121 kb on - strand, within RR42_RS01735 at 396.123 kb on - strand, within RR42_RS01735 at 396.167 kb on + strand, within RR42_RS01735 at 396.560 kb on + strand at 396.560 kb on + strand at 396.560 kb on + strand at 396.560 kb on + strand at 396.560 kb on + strand at 396.560 kb on + strand at 396.560 kb on + strand at 396.560 kb on + strand at 396.560 kb on + strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.561 kb on - strand at 396.566 kb on + strand at 396.566 kb on + strand at 396.567 kb on - strand at 396.567 kb on - strand at 396.567 kb on - strand at 396.567 kb on - strand at 396.570 kb on + strand at 396.570 kb on + strand at 396.570 kb on + strand at 396.570 kb on + strand at 396.570 kb on + strand at 396.571 kb on - strand at 396.571 kb on - strand at 396.604 kb on + strand at 396.604 kb on + strand at 396.605 kb on - strand at 396.605 kb on - strand at 396.605 kb on - strand at 396.605 kb on - strand at 396.605 kb on - strand at 396.609 kb on + strand at 396.610 kb on - strand at 396.610 kb on - strand at 396.815 kb on - strand at 396.845 kb on + strand at 396.919 kb on - strand at 396.948 kb on + strand at 396.948 kb on + strand at 396.948 kb on + strand at 396.948 kb on + strand at 396.949 kb on - strand at 396.949 kb on - strand at 396.949 kb on - strand at 397.516 kb on + strand, within RR42_RS01740 at 397.862 kb on - strand, within RR42_RS01740 at 397.863 kb on + strand, within RR42_RS01740 at 397.863 kb on + strand, within RR42_RS01740 at 397.864 kb on - strand, within RR42_RS01740 at 397.864 kb on - strand, within RR42_RS01740 at 398.163 kb on - strand, within RR42_RS01740 at 398.258 kb on + strand at 398.259 kb on - strand at 398.751 kb on - strand at 398.751 kb on - strand at 398.887 kb on - strand at 398.930 kb on + strand at 398.931 kb on - strand at 398.931 kb on - strand at 398.940 kb on + strand at 398.940 kb on + strand at 398.940 kb on + strand at 398.940 kb on + strand at 398.941 kb on - strand at 399.050 kb on + strand at 399.073 kb on - strand at 399.080 kb on + strand at 399.080 kb on + strand at 399.080 kb on + strand at 399.081 kb on - strand at 399.098 kb on + strand at 399.300 kb on + strand, within RR42_RS01750 at 399.301 kb on - strand, within RR42_RS01750 at 399.301 kb on - strand, within RR42_RS01750 at 399.301 kb on - strand, within RR42_RS01750 at 399.301 kb on - strand, within RR42_RS01750
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 395,861 + +0.4 395,862 - -0.5 395,862 - -0.3 395,862 - +1.0 395,892 + +0.5 395,967 + RR42_RS01735 0.15 +0.3 395,968 - RR42_RS01735 0.15 +0.5 396,111 + RR42_RS01735 0.37 +0.0 396,112 - RR42_RS01735 0.37 +1.0 396,120 + RR42_RS01735 0.39 -0.0 396,120 + RR42_RS01735 0.39 +1.1 396,120 + RR42_RS01735 0.39 -0.3 396,120 + RR42_RS01735 0.39 -1.6 396,120 + RR42_RS01735 0.39 +1.0 396,120 + RR42_RS01735 0.39 +0.2 396,120 + RR42_RS01735 0.39 -1.0 396,120 + RR42_RS01735 0.39 +0.1 396,121 - RR42_RS01735 0.39 +0.2 396,121 - RR42_RS01735 0.39 +0.1 396,121 - RR42_RS01735 0.39 +0.3 396,121 - RR42_RS01735 0.39 -0.1 396,121 - RR42_RS01735 0.39 +1.5 396,121 - RR42_RS01735 0.39 -0.5 396,123 - RR42_RS01735 0.39 +0.2 396,167 + RR42_RS01735 0.46 -0.4 396,560 + -0.1 396,560 + -0.3 396,560 + -0.2 396,560 + +0.5 396,560 + -0.1 396,560 + +0.7 396,560 + +0.4 396,560 + -0.1 396,560 + -0.5 396,561 - +0.2 396,561 - +0.4 396,561 - -0.3 396,561 - +1.2 396,561 - +0.6 396,561 - +1.4 396,561 - -0.7 396,561 - -0.5 396,561 - +0.1 396,561 - -0.5 396,561 - -0.1 396,561 - -0.9 396,561 - -0.5 396,561 - -0.7 396,561 - +0.3 396,566 + -0.5 396,566 + -0.1 396,567 - +1.0 396,567 - +0.4 396,567 - +0.4 396,567 - -0.4 396,570 + -0.1 396,570 + -0.4 396,570 + -0.0 396,570 + +0.1 396,570 + +1.2 396,571 - +0.5 396,571 - +0.6 396,604 + +0.6 396,604 + -0.5 396,605 - +1.2 396,605 - -2.7 396,605 - +0.2 396,605 - +0.2 396,605 - +0.9 396,609 + +0.3 396,610 - -0.0 396,610 - -0.8 396,815 - -1.5 396,845 + -0.3 396,919 - +1.2 396,948 + +0.0 396,948 + -0.1 396,948 + -0.4 396,948 + -0.5 396,949 - -2.0 396,949 - +1.3 396,949 - -0.1 397,516 + RR42_RS01740 0.46 +1.1 397,862 - RR42_RS01740 0.68 +0.6 397,863 + RR42_RS01740 0.68 +0.6 397,863 + RR42_RS01740 0.68 -0.6 397,864 - RR42_RS01740 0.68 +0.4 397,864 - RR42_RS01740 0.68 -0.1 398,163 - RR42_RS01740 0.87 +1.4 398,258 + +0.9 398,259 - +0.3 398,751 - +0.6 398,751 - +0.4 398,887 - +0.4 398,930 + +0.6 398,931 - +0.6 398,931 - +0.1 398,940 + +0.5 398,940 + +0.7 398,940 + +1.7 398,940 + +2.4 398,941 - +0.2 399,050 + +0.2 399,073 - +0.2 399,080 + +0.5 399,080 + +0.6 399,080 + -0.4 399,081 - +0.8 399,098 + -1.6 399,300 + RR42_RS01750 0.21 +0.9 399,301 - RR42_RS01750 0.21 +0.2 399,301 - RR42_RS01750 0.21 +0.0 399,301 - RR42_RS01750 0.21 +0.9 399,301 - RR42_RS01750 0.21 +1.4
Or see this region's nucleotide sequence