Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00450 and RR42_RS00455 are separated by 15 nucleotides RR42_RS00455 and RR42_RS00460 are separated by 62 nucleotides RR42_RS00460 and RR42_RS00465 are separated by 144 nucleotides
RR42_RS00450: RR42_RS00450 - cell division topological specificity factor MinE, at 117,050 to 117,304
_RS00450
RR42_RS00455: RR42_RS00455 - cell division inhibitor MinD, at 117,320 to 118,135
_RS00455
RR42_RS00460: RR42_RS00460 - septum formation inhibitor, at 118,198 to 119,013
_RS00460
RR42_RS00465: RR42_RS00465 - magnesium transporter, at 119,158 to 120,129
_RS00465
Position (kb) 118
119
120 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 117.322 kb on - strand at 117.322 kb on - strand at 117.363 kb on + strand at 117.420 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.589 kb on - strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.735 kb on + strand, within RR42_RS00455 at 117.736 kb on - strand, within RR42_RS00455 at 117.736 kb on - strand, within RR42_RS00455 at 117.736 kb on - strand, within RR42_RS00455 at 117.789 kb on + strand, within RR42_RS00455 at 117.789 kb on + strand, within RR42_RS00455 at 117.790 kb on - strand, within RR42_RS00455 at 117.945 kb on - strand, within RR42_RS00455 at 117.945 kb on - strand, within RR42_RS00455 at 117.963 kb on + strand, within RR42_RS00455 at 118.094 kb on + strand at 118.199 kb on + strand at 118.200 kb on - strand at 118.200 kb on - strand at 118.200 kb on - strand at 118.301 kb on + strand, within RR42_RS00460 at 118.406 kb on + strand, within RR42_RS00460 at 118.407 kb on - strand, within RR42_RS00460 at 118.407 kb on - strand, within RR42_RS00460 at 118.410 kb on + strand, within RR42_RS00460 at 118.410 kb on + strand, within RR42_RS00460 at 118.410 kb on + strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.411 kb on - strand, within RR42_RS00460 at 118.416 kb on + strand, within RR42_RS00460 at 118.445 kb on + strand, within RR42_RS00460 at 118.484 kb on + strand, within RR42_RS00460 at 118.485 kb on - strand, within RR42_RS00460 at 119.013 kb on + strand at 119.013 kb on + strand at 119.013 kb on + strand at 119.013 kb on + strand at 119.014 kb on - strand at 119.042 kb on + strand at 119.043 kb on - strand at 119.074 kb on - strand at 119.126 kb on + strand at 119.126 kb on + strand at 119.127 kb on - strand at 119.186 kb on - strand at 119.383 kb on + strand, within RR42_RS00465 at 119.476 kb on + strand, within RR42_RS00465 at 119.476 kb on + strand, within RR42_RS00465 at 119.476 kb on + strand, within RR42_RS00465 at 119.476 kb on + strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.477 kb on - strand, within RR42_RS00465 at 119.512 kb on + strand, within RR42_RS00465 at 119.512 kb on + strand, within RR42_RS00465 at 119.572 kb on + strand, within RR42_RS00465 at 119.572 kb on + strand, within RR42_RS00465 at 119.573 kb on - strand, within RR42_RS00465 at 119.581 kb on + strand, within RR42_RS00465 at 119.582 kb on - strand, within RR42_RS00465 at 119.611 kb on + strand, within RR42_RS00465 at 119.612 kb on - strand, within RR42_RS00465 at 119.612 kb on - strand, within RR42_RS00465 at 119.821 kb on + strand, within RR42_RS00465 at 120.004 kb on + strand, within RR42_RS00465 at 120.004 kb on + strand, within RR42_RS00465 at 120.004 kb on + strand, within RR42_RS00465
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 117,322 - -1.3 117,322 - -0.4 117,363 + -0.3 117,420 + RR42_RS00455 0.12 -0.6 117,588 + RR42_RS00455 0.33 +0.6 117,588 + RR42_RS00455 0.33 +0.2 117,588 + RR42_RS00455 0.33 -0.8 117,588 + RR42_RS00455 0.33 -0.6 117,589 - RR42_RS00455 0.33 -0.2 117,735 + RR42_RS00455 0.51 +0.9 117,735 + RR42_RS00455 0.51 +0.3 117,735 + RR42_RS00455 0.51 -1.5 117,735 + RR42_RS00455 0.51 -0.8 117,736 - RR42_RS00455 0.51 -0.7 117,736 - RR42_RS00455 0.51 -0.2 117,736 - RR42_RS00455 0.51 -0.9 117,789 + RR42_RS00455 0.57 +0.4 117,789 + RR42_RS00455 0.57 -0.8 117,790 - RR42_RS00455 0.58 -0.5 117,945 - RR42_RS00455 0.77 -0.9 117,945 - RR42_RS00455 0.77 -0.9 117,963 + RR42_RS00455 0.79 -0.1 118,094 + -0.3 118,199 + +0.5 118,200 - -0.3 118,200 - +0.4 118,200 - -0.1 118,301 + RR42_RS00460 0.13 -0.5 118,406 + RR42_RS00460 0.25 -0.2 118,407 - RR42_RS00460 0.26 +0.2 118,407 - RR42_RS00460 0.26 +0.0 118,410 + RR42_RS00460 0.26 -0.8 118,410 + RR42_RS00460 0.26 -1.4 118,410 + RR42_RS00460 0.26 +0.8 118,411 - RR42_RS00460 0.26 -0.7 118,411 - RR42_RS00460 0.26 -2.5 118,411 - RR42_RS00460 0.26 -1.1 118,411 - RR42_RS00460 0.26 -0.6 118,411 - RR42_RS00460 0.26 -0.3 118,416 + RR42_RS00460 0.27 -0.7 118,445 + RR42_RS00460 0.30 +0.7 118,484 + RR42_RS00460 0.35 -0.7 118,485 - RR42_RS00460 0.35 -0.3 119,013 + -0.3 119,013 + +0.4 119,013 + +0.2 119,013 + -1.0 119,014 - +0.1 119,042 + +0.7 119,043 - +0.1 119,074 - -0.6 119,126 + +0.3 119,126 + +1.2 119,127 - +0.7 119,186 - +0.2 119,383 + RR42_RS00465 0.23 -1.8 119,476 + RR42_RS00465 0.33 -0.5 119,476 + RR42_RS00465 0.33 -0.1 119,476 + RR42_RS00465 0.33 +0.8 119,476 + RR42_RS00465 0.33 +1.2 119,477 - RR42_RS00465 0.33 -0.1 119,477 - RR42_RS00465 0.33 +0.3 119,477 - RR42_RS00465 0.33 -0.2 119,477 - RR42_RS00465 0.33 -1.1 119,477 - RR42_RS00465 0.33 +0.0 119,477 - RR42_RS00465 0.33 +0.7 119,477 - RR42_RS00465 0.33 +0.2 119,477 - RR42_RS00465 0.33 -0.5 119,512 + RR42_RS00465 0.36 +0.9 119,512 + RR42_RS00465 0.36 +0.3 119,572 + RR42_RS00465 0.43 -0.6 119,572 + RR42_RS00465 0.43 +0.5 119,573 - RR42_RS00465 0.43 +0.2 119,581 + RR42_RS00465 0.44 -1.5 119,582 - RR42_RS00465 0.44 +0.2 119,611 + RR42_RS00465 0.47 -0.2 119,612 - RR42_RS00465 0.47 +2.8 119,612 - RR42_RS00465 0.47 +0.2 119,821 + RR42_RS00465 0.68 -0.2 120,004 + RR42_RS00465 0.87 -1.6 120,004 + RR42_RS00465 0.87 +0.2 120,004 + RR42_RS00465 0.87 +0.1
Or see this region's nucleotide sequence