Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32225 and RR42_RS32230 are separated by 158 nucleotides RR42_RS32230 and RR42_RS32235 are separated by 33 nucleotides
RR42_RS32225: RR42_RS32225 - NAD-dependent dehydratase, at 2,616,235 to 2,617,236
_RS32225
RR42_RS32230: RR42_RS32230 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 2,617,395 to 2,618,396
_RS32230
RR42_RS32235: RR42_RS32235 - hopanoid biosynthesis associated radical SAM protein HpnH, at 2,618,430 to 2,619,596
_RS32235
Position (kb)
2617
2618
2619 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.545 kb on + strand, within RR42_RS32225 at 2616.546 kb on - strand, within RR42_RS32225 at 2616.546 kb on - strand, within RR42_RS32225 at 2616.546 kb on - strand, within RR42_RS32225 at 2616.583 kb on - strand, within RR42_RS32225 at 2616.583 kb on - strand, within RR42_RS32225 at 2616.583 kb on - strand, within RR42_RS32225 at 2616.583 kb on - strand, within RR42_RS32225 at 2616.583 kb on - strand, within RR42_RS32225 at 2616.664 kb on + strand, within RR42_RS32225 at 2616.664 kb on + strand, within RR42_RS32225 at 2616.665 kb on - strand, within RR42_RS32225 at 2616.804 kb on - strand, within RR42_RS32225 at 2616.896 kb on + strand, within RR42_RS32225 at 2616.897 kb on - strand, within RR42_RS32225 at 2616.897 kb on - strand, within RR42_RS32225 at 2616.897 kb on - strand, within RR42_RS32225 at 2617.002 kb on - strand, within RR42_RS32225 at 2617.025 kb on + strand, within RR42_RS32225 at 2617.025 kb on + strand, within RR42_RS32225 at 2617.025 kb on + strand, within RR42_RS32225 at 2617.026 kb on - strand, within RR42_RS32225 at 2617.026 kb on - strand, within RR42_RS32225 at 2617.220 kb on + strand at 2617.236 kb on + strand at 2617.237 kb on - strand at 2617.237 kb on - strand at 2617.308 kb on + strand at 2617.308 kb on + strand at 2617.309 kb on - strand at 2617.310 kb on + strand at 2617.310 kb on + strand at 2617.310 kb on + strand at 2617.310 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.312 kb on + strand at 2617.313 kb on - strand at 2617.313 kb on - strand at 2617.313 kb on - strand at 2617.313 kb on - strand at 2617.315 kb on + strand at 2617.315 kb on + strand at 2617.316 kb on - strand at 2617.348 kb on + strand at 2617.356 kb on + strand at 2617.357 kb on - strand at 2617.357 kb on - strand at 2617.388 kb on + strand at 2617.388 kb on + strand at 2617.389 kb on - strand at 2617.389 kb on - strand at 2617.394 kb on + strand at 2617.395 kb on - strand at 2617.459 kb on + strand at 2617.460 kb on - strand at 2617.460 kb on - strand at 2617.719 kb on + strand, within RR42_RS32230 at 2617.720 kb on - strand, within RR42_RS32230 at 2617.873 kb on - strand, within RR42_RS32230 at 2617.887 kb on - strand, within RR42_RS32230 at 2617.887 kb on - strand, within RR42_RS32230 at 2617.887 kb on - strand, within RR42_RS32230 at 2617.910 kb on + strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.911 kb on - strand, within RR42_RS32230 at 2617.913 kb on + strand, within RR42_RS32230 at 2617.913 kb on + strand, within RR42_RS32230 at 2617.914 kb on - strand, within RR42_RS32230 at 2617.914 kb on - strand, within RR42_RS32230 at 2617.915 kb on + strand, within RR42_RS32230 at 2617.915 kb on + strand, within RR42_RS32230 at 2617.915 kb on + strand, within RR42_RS32230 at 2617.916 kb on - strand, within RR42_RS32230 at 2617.916 kb on - strand, within RR42_RS32230 at 2617.943 kb on + strand, within RR42_RS32230 at 2617.977 kb on + strand, within RR42_RS32230 at 2618.058 kb on - strand, within RR42_RS32230 at 2618.058 kb on - strand, within RR42_RS32230 at 2618.112 kb on + strand, within RR42_RS32230 at 2618.413 kb on + strand at 2618.425 kb on - strand at 2618.470 kb on - strand at 2618.470 kb on - strand at 2618.555 kb on + strand, within RR42_RS32235 at 2618.784 kb on + strand, within RR42_RS32235 at 2618.785 kb on - strand, within RR42_RS32235 at 2618.872 kb on - strand, within RR42_RS32235 at 2619.030 kb on + strand, within RR42_RS32235 at 2619.186 kb on + strand, within RR42_RS32235 at 2619.187 kb on - strand, within RR42_RS32235 at 2619.187 kb on - strand, within RR42_RS32235 at 2619.246 kb on + strand, within RR42_RS32235 at 2619.246 kb on + strand, within RR42_RS32235 at 2619.265 kb on - strand, within RR42_RS32235 at 2619.312 kb on + strand, within RR42_RS32235 at 2619.313 kb on - strand, within RR42_RS32235 at 2619.357 kb on - strand, within RR42_RS32235
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 2,616,545 + RR42_RS32225 0.31 -0.3 2,616,545 + RR42_RS32225 0.31 -2.6 2,616,545 + RR42_RS32225 0.31 -0.5 2,616,545 + RR42_RS32225 0.31 -0.0 2,616,545 + RR42_RS32225 0.31 -1.2 2,616,545 + RR42_RS32225 0.31 +0.0 2,616,545 + RR42_RS32225 0.31 +0.3 2,616,545 + RR42_RS32225 0.31 -0.1 2,616,546 - RR42_RS32225 0.31 -0.4 2,616,546 - RR42_RS32225 0.31 +0.8 2,616,546 - RR42_RS32225 0.31 +0.5 2,616,583 - RR42_RS32225 0.35 -0.2 2,616,583 - RR42_RS32225 0.35 -0.7 2,616,583 - RR42_RS32225 0.35 -0.5 2,616,583 - RR42_RS32225 0.35 +0.8 2,616,583 - RR42_RS32225 0.35 +0.5 2,616,664 + RR42_RS32225 0.43 +0.6 2,616,664 + RR42_RS32225 0.43 +0.2 2,616,665 - RR42_RS32225 0.43 +0.3 2,616,804 - RR42_RS32225 0.57 +0.6 2,616,896 + RR42_RS32225 0.66 +0.5 2,616,897 - RR42_RS32225 0.66 -0.2 2,616,897 - RR42_RS32225 0.66 +0.3 2,616,897 - RR42_RS32225 0.66 +0.5 2,617,002 - RR42_RS32225 0.77 +1.8 2,617,025 + RR42_RS32225 0.79 +0.8 2,617,025 + RR42_RS32225 0.79 +0.4 2,617,025 + RR42_RS32225 0.79 +0.8 2,617,026 - RR42_RS32225 0.79 +0.4 2,617,026 - RR42_RS32225 0.79 -0.0 2,617,220 + +1.4 2,617,236 + +0.3 2,617,237 - -0.4 2,617,237 - +0.3 2,617,308 + -2.4 2,617,308 + +0.4 2,617,309 - +0.9 2,617,310 + +0.7 2,617,310 + -0.1 2,617,310 + +0.7 2,617,310 + +0.3 2,617,312 + -0.5 2,617,312 + +0.1 2,617,312 + +0.7 2,617,312 + +1.1 2,617,312 + -1.0 2,617,312 + +0.1 2,617,312 + -0.3 2,617,313 - -1.2 2,617,313 - +1.5 2,617,313 - +0.3 2,617,313 - -0.2 2,617,315 + -0.1 2,617,315 + -0.1 2,617,316 - +1.0 2,617,348 + +0.1 2,617,356 + -0.5 2,617,357 - +0.6 2,617,357 - -0.3 2,617,388 + -0.3 2,617,388 + +0.2 2,617,389 - -1.1 2,617,389 - -0.1 2,617,394 + +0.8 2,617,395 - -0.8 2,617,459 + -0.7 2,617,460 - +0.3 2,617,460 - -1.4 2,617,719 + RR42_RS32230 0.32 +0.2 2,617,720 - RR42_RS32230 0.32 +0.7 2,617,873 - RR42_RS32230 0.48 +0.2 2,617,887 - RR42_RS32230 0.49 -0.7 2,617,887 - RR42_RS32230 0.49 +0.8 2,617,887 - RR42_RS32230 0.49 -2.0 2,617,910 + RR42_RS32230 0.51 -0.0 2,617,911 - RR42_RS32230 0.51 +0.1 2,617,911 - RR42_RS32230 0.51 +0.0 2,617,911 - RR42_RS32230 0.51 -0.3 2,617,911 - RR42_RS32230 0.51 -0.4 2,617,913 + RR42_RS32230 0.52 +0.3 2,617,913 + RR42_RS32230 0.52 +0.4 2,617,914 - RR42_RS32230 0.52 -0.8 2,617,914 - RR42_RS32230 0.52 +0.5 2,617,915 + RR42_RS32230 0.52 +0.2 2,617,915 + RR42_RS32230 0.52 -0.3 2,617,915 + RR42_RS32230 0.52 +0.4 2,617,916 - RR42_RS32230 0.52 +0.3 2,617,916 - RR42_RS32230 0.52 -0.3 2,617,943 + RR42_RS32230 0.55 +0.0 2,617,977 + RR42_RS32230 0.58 +0.2 2,618,058 - RR42_RS32230 0.66 -0.6 2,618,058 - RR42_RS32230 0.66 -1.0 2,618,112 + RR42_RS32230 0.72 -0.9 2,618,413 + +1.0 2,618,425 - -2.0 2,618,470 - -1.4 2,618,470 - -0.3 2,618,555 + RR42_RS32235 0.11 -1.5 2,618,784 + RR42_RS32235 0.30 -0.1 2,618,785 - RR42_RS32235 0.30 +0.8 2,618,872 - RR42_RS32235 0.38 -0.3 2,619,030 + RR42_RS32235 0.51 -1.5 2,619,186 + RR42_RS32235 0.65 -0.4 2,619,187 - RR42_RS32235 0.65 +1.0 2,619,187 - RR42_RS32235 0.65 -0.6 2,619,246 + RR42_RS32235 0.70 -1.8 2,619,246 + RR42_RS32235 0.70 -1.9 2,619,265 - RR42_RS32235 0.72 -0.4 2,619,312 + RR42_RS32235 0.76 -1.4 2,619,313 - RR42_RS32235 0.76 -1.2 2,619,357 - RR42_RS32235 0.79 -1.2
Or see this region's nucleotide sequence