Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31940 and RR42_RS31945 overlap by 4 nucleotides RR42_RS31945 and RR42_RS31950 are separated by 8 nucleotides RR42_RS31950 and RR42_RS31955 are separated by 26 nucleotides RR42_RS31955 and RR42_RS31960 are separated by 4 nucleotides
RR42_RS31940: RR42_RS31940 - 2-nitropropane dioxygenase, at 2,542,816 to 2,543,925
_RS31940
RR42_RS31945: RR42_RS31945 - enoyl-CoA hydratase, at 2,543,922 to 2,544,812
_RS31945
RR42_RS31950: RR42_RS31950 - ketoacid CoA transferase, at 2,544,821 to 2,545,624
_RS31950
RR42_RS31955: RR42_RS31955 - CoA-transferase, at 2,545,651 to 2,546,541
_RS31955
RR42_RS31960: RR42_RS31960 - biphenyl 2,3-dioxygenase, at 2,546,546 to 2,547,442
_RS31960
Position (kb)
2544
2545
2546 Strain fitness (log2 ratio)
-1
0
1 at 2543.904 kb on + strand at 2543.904 kb on + strand at 2544.281 kb on - strand, within RR42_RS31945 at 2544.470 kb on - strand, within RR42_RS31945 at 2544.470 kb on - strand, within RR42_RS31945 at 2544.491 kb on - strand, within RR42_RS31945 at 2545.126 kb on - strand, within RR42_RS31950 at 2545.126 kb on - strand, within RR42_RS31950 at 2545.145 kb on - strand, within RR42_RS31950 at 2545.145 kb on - strand, within RR42_RS31950 at 2545.288 kb on + strand, within RR42_RS31950 at 2545.449 kb on + strand, within RR42_RS31950 at 2545.710 kb on - strand at 2545.710 kb on - strand at 2545.746 kb on - strand, within RR42_RS31955 at 2545.784 kb on + strand, within RR42_RS31955 at 2545.814 kb on + strand, within RR42_RS31955 at 2545.955 kb on + strand, within RR42_RS31955 at 2545.955 kb on + strand, within RR42_RS31955 at 2545.955 kb on + strand, within RR42_RS31955 at 2545.956 kb on - strand, within RR42_RS31955 at 2545.956 kb on - strand, within RR42_RS31955 at 2545.956 kb on - strand, within RR42_RS31955 at 2546.467 kb on - strand at 2546.541 kb on + strand at 2546.541 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 2,543,904 + +0.3 2,543,904 + +0.1 2,544,281 - RR42_RS31945 0.40 -0.4 2,544,470 - RR42_RS31945 0.62 -0.4 2,544,470 - RR42_RS31945 0.62 +0.2 2,544,491 - RR42_RS31945 0.64 +0.4 2,545,126 - RR42_RS31950 0.38 +0.7 2,545,126 - RR42_RS31950 0.38 +0.9 2,545,145 - RR42_RS31950 0.40 +0.8 2,545,145 - RR42_RS31950 0.40 -0.8 2,545,288 + RR42_RS31950 0.58 +0.2 2,545,449 + RR42_RS31950 0.78 -0.6 2,545,710 - -0.1 2,545,710 - +0.9 2,545,746 - RR42_RS31955 0.11 -0.2 2,545,784 + RR42_RS31955 0.15 +1.0 2,545,814 + RR42_RS31955 0.18 -0.7 2,545,955 + RR42_RS31955 0.34 +0.5 2,545,955 + RR42_RS31955 0.34 -0.7 2,545,955 + RR42_RS31955 0.34 -0.5 2,545,956 - RR42_RS31955 0.34 +0.4 2,545,956 - RR42_RS31955 0.34 +0.3 2,545,956 - RR42_RS31955 0.34 +0.3 2,546,467 - +0.0 2,546,541 + +0.8 2,546,541 + -0.2
Or see this region's nucleotide sequence