Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29035 and RR42_RS29040 are separated by 346 nucleotides RR42_RS29040 and RR42_RS29045 are separated by 177 nucleotides RR42_RS29045 and RR42_RS29050 are separated by 179 nucleotides
RR42_RS29035: RR42_RS29035 - acyl-CoA synthetase, at 1,868,823 to 1,870,505
_RS29035
RR42_RS29040: RR42_RS29040 - histidyl-tRNA synthetase, at 1,870,852 to 1,871,316
_RS29040
RR42_RS29045: RR42_RS29045 - esterase, at 1,871,494 to 1,872,456
_RS29045
RR42_RS29050: RR42_RS29050 - glutamate synthase, at 1,872,636 to 1,874,225
_RS29050
Position (kb)
1871
1872
1873 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.601 kb on + strand at 1870.602 kb on - strand at 1870.602 kb on - strand at 1870.602 kb on - strand at 1870.603 kb on + strand at 1870.604 kb on - strand at 1870.841 kb on + strand at 1870.841 kb on + strand at 1870.842 kb on - strand at 1870.842 kb on - strand at 1870.845 kb on + strand at 1870.846 kb on - strand at 1870.846 kb on - strand at 1870.852 kb on - strand at 1870.934 kb on + strand, within RR42_RS29040 at 1871.048 kb on + strand, within RR42_RS29040 at 1871.186 kb on + strand, within RR42_RS29040 at 1871.186 kb on + strand, within RR42_RS29040 at 1871.187 kb on - strand, within RR42_RS29040 at 1871.187 kb on - strand, within RR42_RS29040 at 1871.432 kb on + strand at 1871.500 kb on - strand at 1871.684 kb on - strand, within RR42_RS29045 at 1871.684 kb on - strand, within RR42_RS29045 at 1871.728 kb on + strand, within RR42_RS29045 at 1871.729 kb on - strand, within RR42_RS29045 at 1871.830 kb on + strand, within RR42_RS29045 at 1871.887 kb on + strand, within RR42_RS29045 at 1872.127 kb on + strand, within RR42_RS29045 at 1872.128 kb on - strand, within RR42_RS29045 at 1872.172 kb on + strand, within RR42_RS29045 at 1872.172 kb on + strand, within RR42_RS29045 at 1872.172 kb on + strand, within RR42_RS29045 at 1872.173 kb on - strand, within RR42_RS29045 at 1872.173 kb on - strand, within RR42_RS29045 at 1872.173 kb on - strand, within RR42_RS29045 at 1872.173 kb on - strand, within RR42_RS29045 at 1872.251 kb on - strand, within RR42_RS29045 at 1872.289 kb on + strand, within RR42_RS29045 at 1872.289 kb on + strand, within RR42_RS29045 at 1872.293 kb on - strand, within RR42_RS29045 at 1872.293 kb on - strand, within RR42_RS29045 at 1872.299 kb on - strand, within RR42_RS29045 at 1872.299 kb on - strand, within RR42_RS29045 at 1872.454 kb on + strand at 1872.455 kb on - strand at 1872.455 kb on - strand at 1872.501 kb on + strand at 1872.502 kb on - strand at 1872.506 kb on + strand at 1872.507 kb on - strand at 1872.507 kb on - strand at 1872.507 kb on - strand at 1872.507 kb on - strand at 1872.625 kb on + strand at 1872.651 kb on + strand at 1872.868 kb on - strand, within RR42_RS29050 at 1872.868 kb on - strand, within RR42_RS29050 at 1872.927 kb on + strand, within RR42_RS29050 at 1872.928 kb on - strand, within RR42_RS29050 at 1872.928 kb on - strand, within RR42_RS29050 at 1873.054 kb on + strand, within RR42_RS29050 at 1873.055 kb on - strand, within RR42_RS29050 at 1873.083 kb on + strand, within RR42_RS29050 at 1873.083 kb on + strand, within RR42_RS29050 at 1873.182 kb on - strand, within RR42_RS29050 at 1873.215 kb on + strand, within RR42_RS29050 at 1873.216 kb on - strand, within RR42_RS29050 at 1873.436 kb on + strand, within RR42_RS29050
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 1,870,599 + +2.9 1,870,599 + +0.8 1,870,599 + -0.0 1,870,599 + -0.2 1,870,599 + -0.4 1,870,599 + +0.6 1,870,600 - -0.4 1,870,600 - +0.2 1,870,600 - -0.5 1,870,600 - -0.8 1,870,600 - +0.3 1,870,601 + +0.1 1,870,602 - -0.5 1,870,602 - -0.1 1,870,602 - -1.7 1,870,603 + +0.8 1,870,604 - +0.1 1,870,841 + -0.5 1,870,841 + -1.1 1,870,842 - -0.3 1,870,842 - -0.4 1,870,845 + +0.5 1,870,846 - -1.2 1,870,846 - +0.2 1,870,852 - -1.0 1,870,934 + RR42_RS29040 0.18 -1.7 1,871,048 + RR42_RS29040 0.42 -0.3 1,871,186 + RR42_RS29040 0.72 -1.2 1,871,186 + RR42_RS29040 0.72 -0.3 1,871,187 - RR42_RS29040 0.72 -0.4 1,871,187 - RR42_RS29040 0.72 +1.7 1,871,432 + +0.7 1,871,500 - -0.5 1,871,684 - RR42_RS29045 0.20 +0.4 1,871,684 - RR42_RS29045 0.20 +0.4 1,871,728 + RR42_RS29045 0.24 +0.3 1,871,729 - RR42_RS29045 0.24 +0.8 1,871,830 + RR42_RS29045 0.35 +0.7 1,871,887 + RR42_RS29045 0.41 +0.2 1,872,127 + RR42_RS29045 0.66 +0.8 1,872,128 - RR42_RS29045 0.66 -0.8 1,872,172 + RR42_RS29045 0.70 -0.2 1,872,172 + RR42_RS29045 0.70 -0.3 1,872,172 + RR42_RS29045 0.70 +0.5 1,872,173 - RR42_RS29045 0.71 +0.5 1,872,173 - RR42_RS29045 0.71 -0.9 1,872,173 - RR42_RS29045 0.71 -0.4 1,872,173 - RR42_RS29045 0.71 -0.3 1,872,251 - RR42_RS29045 0.79 +0.4 1,872,289 + RR42_RS29045 0.83 +0.5 1,872,289 + RR42_RS29045 0.83 -0.8 1,872,293 - RR42_RS29045 0.83 +0.6 1,872,293 - RR42_RS29045 0.83 +0.3 1,872,299 - RR42_RS29045 0.84 -0.1 1,872,299 - RR42_RS29045 0.84 -1.0 1,872,454 + -0.4 1,872,455 - +0.8 1,872,455 - +0.4 1,872,501 + +1.5 1,872,502 - +0.1 1,872,506 + +0.6 1,872,507 - -0.2 1,872,507 - -1.5 1,872,507 - +0.3 1,872,507 - -0.1 1,872,625 + +0.2 1,872,651 + -0.1 1,872,868 - RR42_RS29050 0.15 -0.2 1,872,868 - RR42_RS29050 0.15 +0.6 1,872,927 + RR42_RS29050 0.18 +0.3 1,872,928 - RR42_RS29050 0.18 +0.2 1,872,928 - RR42_RS29050 0.18 -0.2 1,873,054 + RR42_RS29050 0.26 +0.6 1,873,055 - RR42_RS29050 0.26 +0.9 1,873,083 + RR42_RS29050 0.28 -0.3 1,873,083 + RR42_RS29050 0.28 +0.1 1,873,182 - RR42_RS29050 0.34 -0.6 1,873,215 + RR42_RS29050 0.36 -1.2 1,873,216 - RR42_RS29050 0.36 +0.2 1,873,436 + RR42_RS29050 0.50 -0.2
Or see this region's nucleotide sequence