Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24890 and RR42_RS24895 are separated by 88 nucleotides RR42_RS24895 and RR42_RS24900 are separated by 262 nucleotides RR42_RS24900 and RR42_RS24905 are separated by 95 nucleotides
RR42_RS24890: RR42_RS24890 - acetate--CoA ligase, at 921,767 to 923,695
_RS24890
RR42_RS24895: RR42_RS24895 - enoyl-CoA hydratase, at 923,784 to 924,614
_RS24895
RR42_RS24900: RR42_RS24900 - NAD-dependent dehydratase, at 924,877 to 925,515
_RS24900
RR42_RS24905: RR42_RS24905 - alpha/beta hydrolase, at 925,611 to 926,546
_RS24905
Position (kb) 923
924
925 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 922.870 kb on + strand, within RR42_RS24890 at 922.995 kb on - strand, within RR42_RS24890 at 923.550 kb on + strand at 923.551 kb on - strand at 923.612 kb on + strand at 923.613 kb on - strand at 923.702 kb on - strand at 923.734 kb on + strand at 923.735 kb on - strand at 923.769 kb on + strand at 923.769 kb on + strand at 923.827 kb on - strand at 923.863 kb on + strand at 924.096 kb on + strand, within RR42_RS24895 at 924.097 kb on - strand, within RR42_RS24895 at 924.227 kb on + strand, within RR42_RS24895 at 924.303 kb on + strand, within RR42_RS24895 at 924.459 kb on + strand, within RR42_RS24895 at 924.459 kb on + strand, within RR42_RS24895 at 924.692 kb on + strand at 924.785 kb on + strand at 924.785 kb on + strand at 924.786 kb on - strand at 924.879 kb on - strand at 924.879 kb on - strand at 924.879 kb on - strand at 924.887 kb on + strand at 924.887 kb on + strand at 924.887 kb on + strand at 924.906 kb on + strand at 924.973 kb on - strand, within RR42_RS24900 at 925.088 kb on + strand, within RR42_RS24900 at 925.197 kb on - strand, within RR42_RS24900 at 925.273 kb on + strand, within RR42_RS24900 at 925.429 kb on + strand, within RR42_RS24900 at 925.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 922,870 + RR42_RS24890 0.57 +0.5 922,995 - RR42_RS24890 0.64 +0.3 923,550 + -0.6 923,551 - -0.3 923,612 + +1.5 923,613 - +0.7 923,702 - +0.1 923,734 + -0.6 923,735 - -0.4 923,769 + -1.4 923,769 + -0.5 923,827 - -1.1 923,863 + +0.7 924,096 + RR42_RS24895 0.38 -2.8 924,097 - RR42_RS24895 0.38 +0.4 924,227 + RR42_RS24895 0.53 -0.7 924,303 + RR42_RS24895 0.62 +0.1 924,459 + RR42_RS24895 0.81 -0.6 924,459 + RR42_RS24895 0.81 +0.4 924,692 + -0.1 924,785 + +0.1 924,785 + -0.2 924,786 - -0.1 924,879 - -0.1 924,879 - -0.2 924,879 - +0.4 924,887 + -0.5 924,887 + +1.0 924,887 + -1.1 924,906 + +1.9 924,973 - RR42_RS24900 0.15 -0.5 925,088 + RR42_RS24900 0.33 +1.1 925,197 - RR42_RS24900 0.50 -0.3 925,273 + RR42_RS24900 0.62 -0.3 925,429 + RR42_RS24900 0.86 -0.3 925,613 - +0.8
Or see this region's nucleotide sequence