Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22335 and RR42_RS22340 are separated by 32 nucleotides RR42_RS22340 and RR42_RS22345 are separated by 233 nucleotides RR42_RS22345 and RR42_RS22350 are separated by 150 nucleotides
RR42_RS22335: RR42_RS22335 - hemagglutinin, at 351,215 to 352,492
_RS22335
RR42_RS22340: RR42_RS22340 - peptidase, at 352,525 to 353,808
_RS22340
RR42_RS22345: RR42_RS22345 - UDP-N-acetylmuramate--alanine ligase, at 354,042 to 354,395
_RS22345
RR42_RS22350: RR42_RS22350 - transporter, at 354,546 to 354,974
_RS22350
Position (kb) 352
353
354 Strain fitness (log2 ratio) -2
-1
0
1 at 351.559 kb on + strand, within RR42_RS22335 at 351.559 kb on + strand, within RR42_RS22335 at 351.559 kb on + strand, within RR42_RS22335 at 351.559 kb on + strand, within RR42_RS22335 at 352.031 kb on - strand, within RR42_RS22335 at 352.031 kb on - strand, within RR42_RS22335 at 352.032 kb on - strand, within RR42_RS22335 at 352.150 kb on + strand, within RR42_RS22335 at 352.237 kb on + strand, within RR42_RS22335 at 352.237 kb on + strand, within RR42_RS22335 at 352.238 kb on - strand, within RR42_RS22335 at 352.494 kb on - strand at 352.524 kb on - strand at 352.524 kb on - strand at 352.590 kb on - strand at 352.592 kb on + strand at 352.592 kb on + strand at 352.592 kb on + strand at 352.593 kb on - strand at 352.593 kb on - strand at 352.808 kb on + strand, within RR42_RS22340 at 352.828 kb on + strand, within RR42_RS22340 at 353.088 kb on + strand, within RR42_RS22340 at 353.089 kb on - strand, within RR42_RS22340 at 353.089 kb on - strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.307 kb on + strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.308 kb on - strand, within RR42_RS22340 at 353.376 kb on - strand, within RR42_RS22340 at 353.475 kb on - strand, within RR42_RS22340 at 353.599 kb on + strand, within RR42_RS22340 at 353.638 kb on + strand, within RR42_RS22340 at 353.692 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.960 kb on - strand at 353.960 kb on - strand at 353.960 kb on - strand at 354.068 kb on - strand at 354.206 kb on + strand, within RR42_RS22345 at 354.207 kb on - strand, within RR42_RS22345 at 354.207 kb on - strand, within RR42_RS22345 at 354.318 kb on + strand, within RR42_RS22345 at 354.319 kb on - strand, within RR42_RS22345 at 354.381 kb on + strand at 354.382 kb on - strand at 354.458 kb on + strand at 354.459 kb on - strand at 354.578 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 351,559 + RR42_RS22335 0.27 +0.4 351,559 + RR42_RS22335 0.27 +0.3 351,559 + RR42_RS22335 0.27 +0.9 351,559 + RR42_RS22335 0.27 -0.1 352,031 - RR42_RS22335 0.64 -0.3 352,031 - RR42_RS22335 0.64 +1.0 352,032 - RR42_RS22335 0.64 +0.5 352,150 + RR42_RS22335 0.73 +0.9 352,237 + RR42_RS22335 0.80 -1.1 352,237 + RR42_RS22335 0.80 -2.1 352,238 - RR42_RS22335 0.80 -0.6 352,494 - -0.4 352,524 - -0.2 352,524 - -0.9 352,590 - -0.5 352,592 + +1.0 352,592 + -0.0 352,592 + +0.4 352,593 - +0.0 352,593 - +1.1 352,808 + RR42_RS22340 0.22 +0.7 352,828 + RR42_RS22340 0.24 +0.8 353,088 + RR42_RS22340 0.44 +0.1 353,089 - RR42_RS22340 0.44 +1.1 353,089 - RR42_RS22340 0.44 +1.1 353,307 + RR42_RS22340 0.61 -0.1 353,307 + RR42_RS22340 0.61 -0.2 353,307 + RR42_RS22340 0.61 +0.1 353,307 + RR42_RS22340 0.61 +0.4 353,307 + RR42_RS22340 0.61 -0.1 353,307 + RR42_RS22340 0.61 -0.7 353,307 + RR42_RS22340 0.61 -1.3 353,308 - RR42_RS22340 0.61 +1.2 353,308 - RR42_RS22340 0.61 -0.5 353,308 - RR42_RS22340 0.61 +0.8 353,308 - RR42_RS22340 0.61 +0.3 353,308 - RR42_RS22340 0.61 +0.1 353,308 - RR42_RS22340 0.61 -1.1 353,308 - RR42_RS22340 0.61 +0.6 353,308 - RR42_RS22340 0.61 +0.4 353,376 - RR42_RS22340 0.66 -0.8 353,475 - RR42_RS22340 0.74 -0.1 353,599 + RR42_RS22340 0.84 +0.7 353,638 + RR42_RS22340 0.87 -1.1 353,692 + -0.0 353,959 + -0.0 353,959 + +0.4 353,959 + +0.2 353,959 + -0.2 353,960 - +0.2 353,960 - +1.3 353,960 - +0.6 354,068 - -1.1 354,206 + RR42_RS22345 0.46 -0.0 354,207 - RR42_RS22345 0.47 -1.2 354,207 - RR42_RS22345 0.47 -0.4 354,318 + RR42_RS22345 0.78 -0.3 354,319 - RR42_RS22345 0.78 -1.4 354,381 + +1.1 354,382 - -0.4 354,458 + +0.7 354,459 - -0.3 354,578 + -1.6
Or see this region's nucleotide sequence