Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22005 and RR42_RS22010 are separated by 154 nucleotides RR42_RS22010 and RR42_RS22015 are separated by 25 nucleotides
RR42_RS22005: RR42_RS22005 - transcriptional regulator, at 268,749 to 269,432
_RS22005
RR42_RS22010: RR42_RS22010 - histidine kinase, at 269,587 to 271,233
_RS22010
RR42_RS22015: RR42_RS22015 - histidine kinase, at 271,259 to 273,655
_RS22015
Position (kb)
269
270
271
272 Strain fitness (log2 ratio)
-1
0
1
2 at 268.627 kb on + strand at 268.628 kb on - strand at 268.645 kb on + strand at 268.744 kb on + strand at 268.744 kb on + strand at 268.745 kb on - strand at 268.745 kb on - strand at 268.745 kb on - strand at 268.748 kb on + strand at 268.749 kb on - strand at 269.043 kb on + strand, within RR42_RS22005 at 269.044 kb on - strand, within RR42_RS22005 at 269.044 kb on - strand, within RR42_RS22005 at 269.044 kb on - strand, within RR42_RS22005 at 269.087 kb on - strand, within RR42_RS22005 at 269.154 kb on + strand, within RR42_RS22005 at 269.155 kb on - strand, within RR42_RS22005 at 269.155 kb on - strand, within RR42_RS22005 at 269.155 kb on - strand, within RR42_RS22005 at 269.155 kb on - strand, within RR42_RS22005 at 269.333 kb on + strand, within RR42_RS22005 at 269.339 kb on + strand, within RR42_RS22005 at 269.340 kb on - strand, within RR42_RS22005 at 269.340 kb on - strand, within RR42_RS22005 at 269.340 kb on - strand, within RR42_RS22005 at 269.340 kb on - strand, within RR42_RS22005 at 269.404 kb on - strand at 269.490 kb on - strand at 269.517 kb on + strand at 269.518 kb on - strand at 269.685 kb on + strand at 269.689 kb on + strand at 269.690 kb on - strand at 270.275 kb on + strand, within RR42_RS22010 at 270.535 kb on + strand, within RR42_RS22010 at 270.535 kb on + strand, within RR42_RS22010 at 270.535 kb on + strand, within RR42_RS22010 at 270.628 kb on - strand, within RR42_RS22010 at 270.628 kb on - strand, within RR42_RS22010 at 270.710 kb on - strand, within RR42_RS22010 at 270.988 kb on + strand, within RR42_RS22010 at 271.020 kb on + strand, within RR42_RS22010 at 271.090 kb on + strand at 271.091 kb on - strand at 271.168 kb on + strand at 271.168 kb on + strand at 271.168 kb on + strand at 271.231 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 268,627 + +0.3 268,628 - +1.1 268,645 + +0.3 268,744 + -1.0 268,744 + -0.2 268,745 - -0.3 268,745 - +0.1 268,745 - +0.3 268,748 + -1.4 268,749 - +0.3 269,043 + RR42_RS22005 0.43 +0.3 269,044 - RR42_RS22005 0.43 -0.3 269,044 - RR42_RS22005 0.43 +0.4 269,044 - RR42_RS22005 0.43 -0.2 269,087 - RR42_RS22005 0.49 -0.0 269,154 + RR42_RS22005 0.59 +1.9 269,155 - RR42_RS22005 0.59 +0.1 269,155 - RR42_RS22005 0.59 +0.7 269,155 - RR42_RS22005 0.59 -0.6 269,155 - RR42_RS22005 0.59 +0.9 269,333 + RR42_RS22005 0.85 +0.1 269,339 + RR42_RS22005 0.86 +1.1 269,340 - RR42_RS22005 0.86 +0.1 269,340 - RR42_RS22005 0.86 -0.5 269,340 - RR42_RS22005 0.86 +0.2 269,340 - RR42_RS22005 0.86 +0.1 269,404 - -0.7 269,490 - -0.7 269,517 + +1.0 269,518 - -1.6 269,685 + +0.1 269,689 + +1.6 269,690 - +0.6 270,275 + RR42_RS22010 0.42 +0.4 270,535 + RR42_RS22010 0.58 +0.1 270,535 + RR42_RS22010 0.58 +1.0 270,535 + RR42_RS22010 0.58 +1.2 270,628 - RR42_RS22010 0.63 +0.4 270,628 - RR42_RS22010 0.63 +0.4 270,710 - RR42_RS22010 0.68 +0.7 270,988 + RR42_RS22010 0.85 +0.4 271,020 + RR42_RS22010 0.87 +1.3 271,090 + +1.1 271,091 - -0.6 271,168 + -1.2 271,168 + +0.5 271,168 + -0.1 271,231 + +0.5
Or see this region's nucleotide sequence