Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16235 and RR42_RS16240 are separated by 44 nucleotides RR42_RS16240 and RR42_RS16245 are separated by 190 nucleotides RR42_RS16245 and RR42_RS16250 are separated by 47 nucleotides
RR42_RS16235: RR42_RS16235 - permease, at 3,514,856 to 3,516,010
_RS16235
RR42_RS16240: RR42_RS16240 - cobalamin biosynthesis protein CbiX, at 3,516,055 to 3,516,459
_RS16240
RR42_RS16245: RR42_RS16245 - uroporphyrin-III methyltransferase, at 3,516,650 to 3,517,420
_RS16245
RR42_RS16250: RR42_RS16250 - sulfate adenylyltransferase, at 3,517,468 to 3,518,772
_RS16250
Position (kb)
3516
3517
3518 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3516.009 kb on - strand at 3516.009 kb on - strand at 3516.060 kb on + strand at 3516.060 kb on + strand at 3516.061 kb on - strand at 3516.418 kb on + strand, within RR42_RS16240 at 3516.418 kb on + strand, within RR42_RS16240 at 3516.614 kb on + strand at 3516.615 kb on - strand at 3516.632 kb on + strand at 3516.633 kb on - strand at 3516.633 kb on - strand at 3516.672 kb on + strand at 3516.673 kb on - strand at 3516.673 kb on - strand at 3516.673 kb on - strand at 3516.930 kb on + strand, within RR42_RS16245 at 3516.930 kb on + strand, within RR42_RS16245 at 3516.930 kb on + strand, within RR42_RS16245 at 3516.930 kb on + strand, within RR42_RS16245 at 3516.931 kb on - strand, within RR42_RS16245 at 3516.931 kb on - strand, within RR42_RS16245 at 3516.931 kb on - strand, within RR42_RS16245 at 3516.933 kb on + strand, within RR42_RS16245 at 3516.933 kb on + strand, within RR42_RS16245 at 3516.944 kb on - strand, within RR42_RS16245 at 3517.096 kb on - strand, within RR42_RS16245 at 3517.370 kb on - strand at 3517.407 kb on + strand at 3517.456 kb on + strand at 3517.456 kb on + strand at 3517.456 kb on + strand at 3517.460 kb on + strand at 3517.460 kb on + strand at 3517.460 kb on + strand at 3517.461 kb on - strand at 3517.560 kb on - strand at 3517.560 kb on - strand at 3517.724 kb on + strand, within RR42_RS16250 at 3517.724 kb on + strand, within RR42_RS16250 at 3517.725 kb on - strand, within RR42_RS16250 at 3517.725 kb on - strand, within RR42_RS16250 at 3517.725 kb on - strand, within RR42_RS16250 at 3517.821 kb on + strand, within RR42_RS16250 at 3517.821 kb on + strand, within RR42_RS16250 at 3517.822 kb on - strand, within RR42_RS16250 at 3518.170 kb on - strand, within RR42_RS16250 at 3518.201 kb on + strand, within RR42_RS16250 at 3518.243 kb on + strand, within RR42_RS16250
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 3,516,009 - -1.1 3,516,009 - -1.8 3,516,060 + +0.7 3,516,060 + -2.4 3,516,061 - -4.3 3,516,418 + RR42_RS16240 0.90 -0.9 3,516,418 + RR42_RS16240 0.90 -3.1 3,516,614 + -0.0 3,516,615 - +1.1 3,516,632 + +1.2 3,516,633 - +0.4 3,516,633 - -1.5 3,516,672 + +0.2 3,516,673 - -1.3 3,516,673 - -0.1 3,516,673 - -0.5 3,516,930 + RR42_RS16245 0.36 -1.9 3,516,930 + RR42_RS16245 0.36 -2.8 3,516,930 + RR42_RS16245 0.36 -5.6 3,516,930 + RR42_RS16245 0.36 -0.5 3,516,931 - RR42_RS16245 0.36 -3.7 3,516,931 - RR42_RS16245 0.36 -3.4 3,516,931 - RR42_RS16245 0.36 -3.5 3,516,933 + RR42_RS16245 0.37 -1.5 3,516,933 + RR42_RS16245 0.37 -3.3 3,516,944 - RR42_RS16245 0.38 -2.1 3,517,096 - RR42_RS16245 0.58 -3.3 3,517,370 - -3.9 3,517,407 + -3.0 3,517,456 + -2.7 3,517,456 + -4.1 3,517,456 + -2.8 3,517,460 + -3.0 3,517,460 + -3.1 3,517,460 + -1.6 3,517,461 - +0.2 3,517,560 - -3.6 3,517,560 - -2.8 3,517,724 + RR42_RS16250 0.20 -2.6 3,517,724 + RR42_RS16250 0.20 -2.0 3,517,725 - RR42_RS16250 0.20 -3.1 3,517,725 - RR42_RS16250 0.20 -3.3 3,517,725 - RR42_RS16250 0.20 -3.6 3,517,821 + RR42_RS16250 0.27 -4.2 3,517,821 + RR42_RS16250 0.27 -3.7 3,517,822 - RR42_RS16250 0.27 -2.7 3,518,170 - RR42_RS16250 0.54 -5.7 3,518,201 + RR42_RS16250 0.56 -3.3 3,518,243 + RR42_RS16250 0.59 -4.4
Or see this region's nucleotide sequence