Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13545 and RR42_RS13550 are separated by 52 nucleotides RR42_RS13550 and RR42_RS13555 are separated by 2 nucleotides RR42_RS13555 and RR42_RS13560 are separated by 193 nucleotides
RR42_RS13545: RR42_RS13545 - enoyl-CoA hydratase, at 2,923,816 to 2,924,667
_RS13545
RR42_RS13550: RR42_RS13550 - 3-hydroxyacyl-CoA dehydrogenase, at 2,924,720 to 2,925,520
_RS13550
RR42_RS13555: RR42_RS13555 - anthraniloyl-CoA monooxygenase (EC 1.14.13.40) (from data) , at 2,925,523 to 2,927,913
_RS13555
RR42_RS13560: RR42_RS13560 - propionyl-CoA synthetase, at 2,928,107 to 2,929,999
_RS13560
Position (kb)
2925
2926
2927
2928 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2924.656 kb on + strand at 2924.767 kb on + strand at 2924.942 kb on - strand, within RR42_RS13550 at 2925.051 kb on + strand, within RR42_RS13550 at 2925.051 kb on + strand, within RR42_RS13550 at 2925.052 kb on - strand, within RR42_RS13550 at 2925.058 kb on - strand, within RR42_RS13550 at 2925.091 kb on + strand, within RR42_RS13550 at 2925.092 kb on - strand, within RR42_RS13550 at 2925.608 kb on + strand at 2925.609 kb on - strand at 2925.609 kb on - strand at 2925.822 kb on - strand, within RR42_RS13555 at 2926.035 kb on - strand, within RR42_RS13555 at 2926.064 kb on + strand, within RR42_RS13555 at 2926.115 kb on + strand, within RR42_RS13555 at 2926.206 kb on + strand, within RR42_RS13555 at 2926.251 kb on + strand, within RR42_RS13555 at 2926.251 kb on + strand, within RR42_RS13555 at 2926.252 kb on - strand, within RR42_RS13555 at 2926.253 kb on + strand, within RR42_RS13555 at 2926.254 kb on - strand, within RR42_RS13555 at 2926.580 kb on + strand, within RR42_RS13555 at 2926.580 kb on + strand, within RR42_RS13555 at 2926.580 kb on + strand, within RR42_RS13555 at 2926.581 kb on - strand, within RR42_RS13555 at 2926.581 kb on - strand, within RR42_RS13555 at 2926.581 kb on - strand, within RR42_RS13555 at 2926.909 kb on + strand, within RR42_RS13555 at 2926.910 kb on - strand, within RR42_RS13555 at 2926.910 kb on - strand, within RR42_RS13555 at 2926.910 kb on - strand, within RR42_RS13555 at 2927.336 kb on + strand, within RR42_RS13555 at 2927.385 kb on + strand, within RR42_RS13555 at 2927.494 kb on - strand, within RR42_RS13555 at 2927.525 kb on + strand, within RR42_RS13555 at 2927.525 kb on + strand, within RR42_RS13555 at 2927.804 kb on + strand at 2927.804 kb on + strand at 2927.829 kb on - strand at 2927.874 kb on - strand at 2928.425 kb on + strand, within RR42_RS13560 at 2928.532 kb on + strand, within RR42_RS13560 at 2928.534 kb on + strand, within RR42_RS13560 at 2928.534 kb on + strand, within RR42_RS13560 at 2928.534 kb on + strand, within RR42_RS13560 at 2928.535 kb on - strand, within RR42_RS13560 at 2928.573 kb on + strand, within RR42_RS13560 at 2928.603 kb on + strand, within RR42_RS13560 at 2928.603 kb on + strand, within RR42_RS13560 at 2928.604 kb on - strand, within RR42_RS13560 at 2928.604 kb on - strand, within RR42_RS13560 at 2928.635 kb on + strand, within RR42_RS13560 at 2928.635 kb on + strand, within RR42_RS13560 at 2928.747 kb on + strand, within RR42_RS13560 at 2928.840 kb on + strand, within RR42_RS13560 at 2928.840 kb on + strand, within RR42_RS13560 at 2928.840 kb on + strand, within RR42_RS13560 at 2928.841 kb on - strand, within RR42_RS13560 at 2928.841 kb on - strand, within RR42_RS13560
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 2,924,656 + -0.9 2,924,767 + +2.5 2,924,942 - RR42_RS13550 0.28 -3.2 2,925,051 + RR42_RS13550 0.41 -1.0 2,925,051 + RR42_RS13550 0.41 -1.6 2,925,052 - RR42_RS13550 0.41 -4.8 2,925,058 - RR42_RS13550 0.42 -1.7 2,925,091 + RR42_RS13550 0.46 -1.2 2,925,092 - RR42_RS13550 0.46 -2.6 2,925,608 + +0.6 2,925,609 - -1.4 2,925,609 - -2.0 2,925,822 - RR42_RS13555 0.13 -0.6 2,926,035 - RR42_RS13555 0.21 -1.7 2,926,064 + RR42_RS13555 0.23 -2.1 2,926,115 + RR42_RS13555 0.25 -1.4 2,926,206 + RR42_RS13555 0.29 -1.2 2,926,251 + RR42_RS13555 0.30 -1.2 2,926,251 + RR42_RS13555 0.30 -2.0 2,926,252 - RR42_RS13555 0.30 -3.2 2,926,253 + RR42_RS13555 0.31 -1.9 2,926,254 - RR42_RS13555 0.31 -4.0 2,926,580 + RR42_RS13555 0.44 -1.4 2,926,580 + RR42_RS13555 0.44 -2.3 2,926,580 + RR42_RS13555 0.44 -2.2 2,926,581 - RR42_RS13555 0.44 -3.3 2,926,581 - RR42_RS13555 0.44 -3.8 2,926,581 - RR42_RS13555 0.44 -2.5 2,926,909 + RR42_RS13555 0.58 -2.7 2,926,910 - RR42_RS13555 0.58 -1.7 2,926,910 - RR42_RS13555 0.58 -3.4 2,926,910 - RR42_RS13555 0.58 -2.1 2,927,336 + RR42_RS13555 0.76 -1.8 2,927,385 + RR42_RS13555 0.78 -0.7 2,927,494 - RR42_RS13555 0.82 -1.8 2,927,525 + RR42_RS13555 0.84 -1.0 2,927,525 + RR42_RS13555 0.84 -2.9 2,927,804 + -1.7 2,927,804 + -0.8 2,927,829 - -1.6 2,927,874 - -1.1 2,928,425 + RR42_RS13560 0.17 +1.0 2,928,532 + RR42_RS13560 0.22 +1.1 2,928,534 + RR42_RS13560 0.23 +0.7 2,928,534 + RR42_RS13560 0.23 -0.0 2,928,534 + RR42_RS13560 0.23 -0.3 2,928,535 - RR42_RS13560 0.23 +0.9 2,928,573 + RR42_RS13560 0.25 +0.2 2,928,603 + RR42_RS13560 0.26 +0.9 2,928,603 + RR42_RS13560 0.26 +0.6 2,928,604 - RR42_RS13560 0.26 +0.2 2,928,604 - RR42_RS13560 0.26 +0.6 2,928,635 + RR42_RS13560 0.28 -0.6 2,928,635 + RR42_RS13560 0.28 +0.4 2,928,747 + RR42_RS13560 0.34 +0.4 2,928,840 + RR42_RS13560 0.39 +0.1 2,928,840 + RR42_RS13560 0.39 -0.9 2,928,840 + RR42_RS13560 0.39 +0.3 2,928,841 - RR42_RS13560 0.39 +0.7 2,928,841 - RR42_RS13560 0.39 -2.7
Or see this region's nucleotide sequence