Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06015 and RR42_RS06020 are separated by 80 nucleotides RR42_RS06020 and RR42_RS06025 are separated by 68 nucleotides
RR42_RS06015: RR42_RS06015 - recombinase, at 1,321,784 to 1,323,874
_RS06015
RR42_RS06020: RR42_RS06020 - Fe-S cluster assembly transcriptional regulator IscR, at 1,323,955 to 1,324,509
_RS06020
RR42_RS06025: RR42_RS06025 - cysteine desulfurase, at 1,324,578 to 1,325,795
_RS06025
Position (kb)
1323
1324
1325 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1322.996 kb on + strand, within RR42_RS06015 at 1322.997 kb on - strand, within RR42_RS06015 at 1322.997 kb on - strand, within RR42_RS06015 at 1322.997 kb on - strand, within RR42_RS06015 at 1322.997 kb on - strand, within RR42_RS06015 at 1322.997 kb on - strand, within RR42_RS06015 at 1323.134 kb on - strand, within RR42_RS06015 at 1323.134 kb on - strand, within RR42_RS06015 at 1323.134 kb on - strand, within RR42_RS06015 at 1323.134 kb on - strand, within RR42_RS06015 at 1323.134 kb on - strand, within RR42_RS06015 at 1323.331 kb on - strand, within RR42_RS06015 at 1323.410 kb on + strand, within RR42_RS06015 at 1323.410 kb on + strand, within RR42_RS06015 at 1323.411 kb on - strand, within RR42_RS06015 at 1323.411 kb on - strand, within RR42_RS06015 at 1323.411 kb on - strand, within RR42_RS06015 at 1323.428 kb on + strand, within RR42_RS06015 at 1323.428 kb on + strand, within RR42_RS06015 at 1323.429 kb on - strand, within RR42_RS06015 at 1323.481 kb on + strand, within RR42_RS06015 at 1323.481 kb on + strand, within RR42_RS06015 at 1323.482 kb on - strand, within RR42_RS06015 at 1323.482 kb on - strand, within RR42_RS06015 at 1324.248 kb on + strand, within RR42_RS06020
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 1,322,996 + RR42_RS06015 0.58 -2.1 1,322,997 - RR42_RS06015 0.58 -3.2 1,322,997 - RR42_RS06015 0.58 -0.7 1,322,997 - RR42_RS06015 0.58 +0.1 1,322,997 - RR42_RS06015 0.58 +0.3 1,322,997 - RR42_RS06015 0.58 -1.4 1,323,134 - RR42_RS06015 0.65 -0.1 1,323,134 - RR42_RS06015 0.65 -1.4 1,323,134 - RR42_RS06015 0.65 +0.1 1,323,134 - RR42_RS06015 0.65 -0.5 1,323,134 - RR42_RS06015 0.65 -1.7 1,323,331 - RR42_RS06015 0.74 -0.1 1,323,410 + RR42_RS06015 0.78 -1.3 1,323,410 + RR42_RS06015 0.78 -1.2 1,323,411 - RR42_RS06015 0.78 -0.8 1,323,411 - RR42_RS06015 0.78 -1.0 1,323,411 - RR42_RS06015 0.78 +0.5 1,323,428 + RR42_RS06015 0.79 -0.3 1,323,428 + RR42_RS06015 0.79 -0.8 1,323,429 - RR42_RS06015 0.79 -0.7 1,323,481 + RR42_RS06015 0.81 -2.6 1,323,481 + RR42_RS06015 0.81 -0.4 1,323,482 - RR42_RS06015 0.81 -0.8 1,323,482 - RR42_RS06015 0.81 +0.3 1,324,248 + RR42_RS06020 0.53 -1.5
Or see this region's nucleotide sequence