Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01905 and RR42_RS01910 are separated by 109 nucleotides RR42_RS01910 and RR42_RS01915 are separated by 7 nucleotides RR42_RS01915 and RR42_RS01920 overlap by 4 nucleotides
RR42_RS01905: RR42_RS01905 - cytochrome C oxidase subunit II, at 428,087 to 428,848
_RS01905
RR42_RS01910: RR42_RS01910 - cytochrome C oxidase subunit I, at 428,958 to 429,614
_RS01910
RR42_RS01915: RR42_RS01915 - cytochrome C oxidase subunit I, at 429,622 to 430,725
_RS01915
RR42_RS01920: RR42_RS01920 - protoheme IX farnesyltransferase, at 430,722 to 431,657
_RS01920
Position (kb) 429
430
431 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 428.640 kb on - strand, within RR42_RS01905 at 428.640 kb on - strand, within RR42_RS01905 at 428.753 kb on + strand, within RR42_RS01905 at 428.754 kb on - strand, within RR42_RS01905 at 428.760 kb on - strand, within RR42_RS01905 at 428.957 kb on + strand at 428.957 kb on + strand at 428.957 kb on + strand at 428.957 kb on + strand at 428.958 kb on - strand at 428.958 kb on - strand at 428.958 kb on - strand at 428.958 kb on - strand at 429.096 kb on + strand, within RR42_RS01910 at 429.105 kb on + strand, within RR42_RS01910 at 429.105 kb on + strand, within RR42_RS01910 at 429.105 kb on + strand, within RR42_RS01910 at 429.106 kb on - strand, within RR42_RS01910 at 429.108 kb on + strand, within RR42_RS01910 at 429.108 kb on + strand, within RR42_RS01910 at 429.108 kb on + strand, within RR42_RS01910 at 429.109 kb on - strand, within RR42_RS01910 at 429.109 kb on - strand, within RR42_RS01910 at 429.109 kb on - strand, within RR42_RS01910 at 429.141 kb on + strand, within RR42_RS01910 at 429.141 kb on + strand, within RR42_RS01910 at 429.142 kb on - strand, within RR42_RS01910 at 429.142 kb on - strand, within RR42_RS01910 at 429.154 kb on - strand, within RR42_RS01910 at 429.403 kb on - strand, within RR42_RS01910 at 429.597 kb on + strand at 429.598 kb on - strand at 429.676 kb on + strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.818 kb on - strand, within RR42_RS01915 at 429.929 kb on - strand, within RR42_RS01915 at 429.929 kb on - strand, within RR42_RS01915 at 430.273 kb on - strand, within RR42_RS01915 at 430.276 kb on + strand, within RR42_RS01915 at 430.276 kb on + strand, within RR42_RS01915 at 430.393 kb on + strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.655 kb on - strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.661 kb on + strand at 430.661 kb on + strand at 430.661 kb on + strand at 430.662 kb on - strand at 430.754 kb on + strand at 431.094 kb on + strand, within RR42_RS01920 at 431.155 kb on - strand, within RR42_RS01920 at 431.155 kb on - strand, within RR42_RS01920 at 431.426 kb on + strand, within RR42_RS01920 at 431.466 kb on - strand, within RR42_RS01920 at 431.481 kb on + strand, within RR42_RS01920 at 431.548 kb on - strand, within RR42_RS01920 at 431.592 kb on + strand at 431.592 kb on + strand at 431.592 kb on + strand at 431.631 kb on + strand at 431.631 kb on + strand at 431.632 kb on - strand at 431.632 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 428,640 - RR42_RS01905 0.73 -0.7 428,640 - RR42_RS01905 0.73 -1.7 428,753 + RR42_RS01905 0.87 -2.4 428,754 - RR42_RS01905 0.88 -0.2 428,760 - RR42_RS01905 0.88 -1.2 428,957 + -0.5 428,957 + -0.8 428,957 + +0.6 428,957 + +0.6 428,958 - -0.8 428,958 - -2.0 428,958 - +0.1 428,958 - -0.4 429,096 + RR42_RS01910 0.21 -0.3 429,105 + RR42_RS01910 0.22 -0.2 429,105 + RR42_RS01910 0.22 -1.7 429,105 + RR42_RS01910 0.22 +0.5 429,106 - RR42_RS01910 0.23 -0.9 429,108 + RR42_RS01910 0.23 -0.7 429,108 + RR42_RS01910 0.23 -0.8 429,108 + RR42_RS01910 0.23 -1.0 429,109 - RR42_RS01910 0.23 -3.2 429,109 - RR42_RS01910 0.23 -0.8 429,109 - RR42_RS01910 0.23 +1.9 429,141 + RR42_RS01910 0.28 -0.1 429,141 + RR42_RS01910 0.28 -1.1 429,142 - RR42_RS01910 0.28 -0.9 429,142 - RR42_RS01910 0.28 -2.2 429,154 - RR42_RS01910 0.30 -3.5 429,403 - RR42_RS01910 0.68 -1.5 429,597 + -2.3 429,598 - -1.0 429,676 + +1.3 429,677 - -1.0 429,677 - -0.3 429,677 - -0.7 429,677 - -0.3 429,677 - -1.0 429,677 - -0.8 429,818 - RR42_RS01915 0.18 -1.0 429,929 - RR42_RS01915 0.28 -0.6 429,929 - RR42_RS01915 0.28 -0.9 430,273 - RR42_RS01915 0.59 -0.5 430,276 + RR42_RS01915 0.59 -0.4 430,276 + RR42_RS01915 0.59 -1.2 430,393 + RR42_RS01915 0.70 -1.4 430,394 - RR42_RS01915 0.70 -2.4 430,394 - RR42_RS01915 0.70 -1.5 430,394 - RR42_RS01915 0.70 -0.1 430,655 - -0.6 430,659 + -0.4 430,659 + +0.9 430,659 + +0.3 430,659 + +0.1 430,659 + -1.0 430,661 + +0.1 430,661 + -0.5 430,661 + +0.4 430,662 - -0.4 430,754 + -1.0 431,094 + RR42_RS01920 0.40 -1.2 431,155 - RR42_RS01920 0.46 -1.4 431,155 - RR42_RS01920 0.46 -1.2 431,426 + RR42_RS01920 0.75 -1.8 431,466 - RR42_RS01920 0.79 +0.2 431,481 + RR42_RS01920 0.81 -1.8 431,548 - RR42_RS01920 0.88 +0.4 431,592 + -2.4 431,592 + -1.0 431,592 + -3.4 431,631 + +0.8 431,631 + +0.0 431,632 - +0.5 431,632 - -0.6
Or see this region's nucleotide sequence