Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01390 and RR42_RS01395 are separated by 31 nucleotides RR42_RS01395 and RR42_RS01400 are separated by 22 nucleotides RR42_RS01400 and RR42_RS01405 are separated by 153 nucleotides
RR42_RS01390: RR42_RS01390 - competence protein, at 314,314 to 314,676
_RS01390
RR42_RS01395: RR42_RS01395 - twitching motility protein PilT, at 314,708 to 315,475
_RS01395
RR42_RS01400: RR42_RS01400 - 5-formyltetrahydrofolate cyclo-ligase, at 315,498 to 316,049
_RS01400
RR42_RS01405: RR42_RS01405 - lytic transglycosylase, at 316,203 to 318,155
_RS01405
Position (kb) 315
316
317 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 314.572 kb on + strand, within RR42_RS01390 at 314.573 kb on - strand, within RR42_RS01390 at 314.697 kb on + strand at 314.697 kb on + strand at 314.751 kb on - strand at 314.945 kb on + strand, within RR42_RS01395 at 315.083 kb on + strand, within RR42_RS01395 at 315.084 kb on - strand, within RR42_RS01395 at 315.095 kb on + strand, within RR42_RS01395 at 315.096 kb on - strand, within RR42_RS01395 at 315.096 kb on - strand, within RR42_RS01395 at 315.197 kb on + strand, within RR42_RS01395 at 315.198 kb on - strand, within RR42_RS01395 at 315.198 kb on - strand, within RR42_RS01395 at 315.430 kb on + strand at 315.430 kb on + strand at 315.430 kb on + strand at 315.430 kb on + strand at 315.431 kb on - strand at 315.431 kb on - strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.474 kb on - strand at 315.644 kb on - strand, within RR42_RS01400 at 315.674 kb on + strand, within RR42_RS01400 at 315.675 kb on - strand, within RR42_RS01400 at 315.708 kb on + strand, within RR42_RS01400 at 315.709 kb on - strand, within RR42_RS01400 at 315.754 kb on + strand, within RR42_RS01400 at 315.755 kb on - strand, within RR42_RS01400 at 315.913 kb on + strand, within RR42_RS01400 at 315.914 kb on - strand, within RR42_RS01400 at 315.914 kb on - strand, within RR42_RS01400 at 316.119 kb on + strand at 316.120 kb on - strand at 316.216 kb on + strand at 316.216 kb on + strand at 316.217 kb on - strand at 316.219 kb on - strand at 316.219 kb on - strand at 316.219 kb on - strand at 316.282 kb on + strand at 316.283 kb on - strand at 316.431 kb on + strand, within RR42_RS01405 at 316.446 kb on + strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.455 kb on + strand, within RR42_RS01405 at 316.455 kb on + strand, within RR42_RS01405 at 316.545 kb on + strand, within RR42_RS01405 at 316.546 kb on - strand, within RR42_RS01405 at 316.678 kb on - strand, within RR42_RS01405 at 316.752 kb on + strand, within RR42_RS01405 at 316.756 kb on - strand, within RR42_RS01405 at 316.785 kb on + strand, within RR42_RS01405 at 316.857 kb on + strand, within RR42_RS01405 at 316.857 kb on + strand, within RR42_RS01405 at 316.857 kb on + strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.858 kb on - strand, within RR42_RS01405 at 316.972 kb on - strand, within RR42_RS01405
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 314,572 + RR42_RS01390 0.71 +0.5 314,573 - RR42_RS01390 0.71 -0.5 314,697 + +1.0 314,697 + -0.3 314,751 - -0.5 314,945 + RR42_RS01395 0.31 -0.6 315,083 + RR42_RS01395 0.49 -0.0 315,084 - RR42_RS01395 0.49 +0.3 315,095 + RR42_RS01395 0.50 +0.5 315,096 - RR42_RS01395 0.51 -0.0 315,096 - RR42_RS01395 0.51 +0.0 315,197 + RR42_RS01395 0.64 +1.3 315,198 - RR42_RS01395 0.64 -0.3 315,198 - RR42_RS01395 0.64 -0.7 315,430 + -1.0 315,430 + -0.6 315,430 + -0.4 315,430 + +0.9 315,431 - -0.8 315,431 - +0.1 315,473 + +1.2 315,473 + -0.5 315,473 + -0.4 315,473 + -0.3 315,474 - +0.6 315,644 - RR42_RS01400 0.26 -1.2 315,674 + RR42_RS01400 0.32 -0.4 315,675 - RR42_RS01400 0.32 -1.2 315,708 + RR42_RS01400 0.38 -1.4 315,709 - RR42_RS01400 0.38 -1.6 315,754 + RR42_RS01400 0.46 -1.4 315,755 - RR42_RS01400 0.47 -2.1 315,913 + RR42_RS01400 0.75 -0.9 315,914 - RR42_RS01400 0.75 -1.5 315,914 - RR42_RS01400 0.75 -0.5 316,119 + -0.9 316,120 - +0.5 316,216 + +0.2 316,216 + -0.7 316,217 - +0.1 316,219 - +0.4 316,219 - +0.1 316,219 - -1.3 316,282 + -0.1 316,283 - -0.3 316,431 + RR42_RS01405 0.12 +0.3 316,446 + RR42_RS01405 0.12 +1.6 316,447 - RR42_RS01405 0.12 -0.2 316,447 - RR42_RS01405 0.12 -1.4 316,447 - RR42_RS01405 0.12 +0.9 316,447 - RR42_RS01405 0.12 +1.2 316,455 + RR42_RS01405 0.13 +0.7 316,455 + RR42_RS01405 0.13 -0.5 316,545 + RR42_RS01405 0.18 -1.6 316,546 - RR42_RS01405 0.18 +0.5 316,678 - RR42_RS01405 0.24 -0.2 316,752 + RR42_RS01405 0.28 -0.3 316,756 - RR42_RS01405 0.28 +0.5 316,785 + RR42_RS01405 0.30 -1.3 316,857 + RR42_RS01405 0.33 +0.8 316,857 + RR42_RS01405 0.33 +0.0 316,857 + RR42_RS01405 0.33 -1.5 316,858 - RR42_RS01405 0.34 -0.1 316,858 - RR42_RS01405 0.34 -0.5 316,858 - RR42_RS01405 0.34 +0.3 316,858 - RR42_RS01405 0.34 +0.1 316,858 - RR42_RS01405 0.34 +0.1 316,858 - RR42_RS01405 0.34 +3.8 316,972 - RR42_RS01405 0.39 +0.2
Or see this region's nucleotide sequence