Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01350 and RR42_RS01355 overlap by 1 nucleotides RR42_RS01355 and RR42_RS01360 are separated by 48 nucleotides RR42_RS01360 and RR42_RS01365 are separated by 4 nucleotides
RR42_RS01350: RR42_RS01350 - biotin carboxylase, at 307,764 to 309,137
_RS01350
RR42_RS01355: RR42_RS01355 - biotin attachment protein, at 309,137 to 309,349
_RS01355
RR42_RS01360: RR42_RS01360 - phenylacetic acid degradation protein, at 309,398 to 309,826
_RS01360
RR42_RS01365: RR42_RS01365 - hypothetical protein, at 309,831 to 310,271
_RS01365
Position (kb) 309
310 Strain fitness (log2 ratio) -2
-1
0
1 at 308.775 kb on + strand, within RR42_RS01350 at 308.776 kb on - strand, within RR42_RS01350 at 308.776 kb on - strand, within RR42_RS01350 at 308.825 kb on - strand, within RR42_RS01350 at 308.896 kb on - strand, within RR42_RS01350 at 308.896 kb on - strand, within RR42_RS01350 at 308.898 kb on + strand, within RR42_RS01350 at 308.898 kb on + strand, within RR42_RS01350 at 308.898 kb on + strand, within RR42_RS01350 at 309.008 kb on + strand at 309.120 kb on - strand at 309.159 kb on + strand, within RR42_RS01355 at 309.159 kb on + strand, within RR42_RS01355 at 309.269 kb on + strand, within RR42_RS01355 at 309.270 kb on - strand, within RR42_RS01355 at 309.348 kb on - strand at 309.434 kb on + strand at 309.649 kb on + strand, within RR42_RS01360 at 309.649 kb on + strand, within RR42_RS01360 at 309.650 kb on - strand, within RR42_RS01360 at 309.650 kb on - strand, within RR42_RS01360 at 309.892 kb on + strand, within RR42_RS01365 at 310.063 kb on + strand, within RR42_RS01365 at 310.194 kb on + strand, within RR42_RS01365 at 310.195 kb on - strand, within RR42_RS01365 at 310.203 kb on + strand, within RR42_RS01365 at 310.204 kb on - strand, within RR42_RS01365 at 310.219 kb on + strand, within RR42_RS01365 at 310.338 kb on + strand at 310.339 kb on - strand at 310.339 kb on - strand at 310.343 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 308,775 + RR42_RS01350 0.74 +0.7 308,776 - RR42_RS01350 0.74 -0.4 308,776 - RR42_RS01350 0.74 -0.8 308,825 - RR42_RS01350 0.77 -1.4 308,896 - RR42_RS01350 0.82 -0.8 308,896 - RR42_RS01350 0.82 +0.2 308,898 + RR42_RS01350 0.83 +0.1 308,898 + RR42_RS01350 0.83 -0.4 308,898 + RR42_RS01350 0.83 +0.2 309,008 + -0.3 309,120 - -0.5 309,159 + RR42_RS01355 0.10 +0.2 309,159 + RR42_RS01355 0.10 -0.8 309,269 + RR42_RS01355 0.62 -0.3 309,270 - RR42_RS01355 0.62 -0.1 309,348 - -0.2 309,434 + -2.1 309,649 + RR42_RS01360 0.59 +1.2 309,649 + RR42_RS01360 0.59 +0.1 309,650 - RR42_RS01360 0.59 +0.3 309,650 - RR42_RS01360 0.59 +0.5 309,892 + RR42_RS01365 0.14 -0.5 310,063 + RR42_RS01365 0.53 -0.3 310,194 + RR42_RS01365 0.82 +0.0 310,195 - RR42_RS01365 0.83 -1.1 310,203 + RR42_RS01365 0.84 +0.1 310,204 - RR42_RS01365 0.85 -1.4 310,219 + RR42_RS01365 0.88 -0.6 310,338 + +0.3 310,339 - +1.1 310,339 - +0.6 310,343 - -0.2
Or see this region's nucleotide sequence