Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08440 and MMJJ_RS08445 are separated by 50 nucleotides MMJJ_RS08445 and MMJJ_RS08450 are separated by 152 nucleotides MMJJ_RS08450 and MMJJ_RS08455 are separated by 15 nucleotides MMJJ_RS08455 and MMJJ_RS08460 are separated by 21 nucleotides
MMJJ_RS08440: MMJJ_RS08440 - amidophosphoribosyltransferase, at 1,552,214 to 1,553,593
_RS08440
MMJJ_RS08445: MMJJ_RS08445 - cell division ATPase MinD, at 1,553,644 to 1,554,432
_RS08445
MMJJ_RS08450: MMJJ_RS08450 - undecaprenyl-diphosphate phosphatase, at 1,554,585 to 1,555,334
_RS08450
MMJJ_RS08455: MMJJ_RS08455 - tetratricopeptide repeat protein, at 1,555,350 to 1,555,730
_RS08455
MMJJ_RS08460: MMJJ_RS08460 - metallophosphoesterase, at 1,555,752 to 1,556,462
_RS08460
Position (kb)
1554
1555
1556 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1553.642 kb on + strand at 1553.703 kb on + strand at 1553.725 kb on + strand, within MMJJ_RS08445 at 1553.732 kb on - strand, within MMJJ_RS08445 at 1553.732 kb on - strand, within MMJJ_RS08445 at 1553.733 kb on + strand, within MMJJ_RS08445 at 1553.750 kb on + strand, within MMJJ_RS08445 at 1553.764 kb on + strand, within MMJJ_RS08445 at 1553.804 kb on + strand, within MMJJ_RS08445 at 1553.818 kb on + strand, within MMJJ_RS08445 at 1553.929 kb on + strand, within MMJJ_RS08445 at 1553.948 kb on + strand, within MMJJ_RS08445 at 1554.097 kb on - strand, within MMJJ_RS08445 at 1554.097 kb on - strand, within MMJJ_RS08445 at 1554.127 kb on - strand, within MMJJ_RS08445 at 1554.175 kb on + strand, within MMJJ_RS08445 at 1554.210 kb on - strand, within MMJJ_RS08445 at 1554.210 kb on - strand, within MMJJ_RS08445 at 1554.210 kb on - strand, within MMJJ_RS08445 at 1554.211 kb on + strand, within MMJJ_RS08445 at 1554.219 kb on + strand, within MMJJ_RS08445 at 1554.455 kb on - strand at 1554.455 kb on - strand at 1554.465 kb on + strand at 1554.472 kb on - strand at 1554.472 kb on - strand at 1554.473 kb on + strand at 1554.476 kb on - strand at 1554.477 kb on + strand at 1554.500 kb on + strand at 1554.500 kb on + strand at 1554.504 kb on + strand at 1554.504 kb on + strand at 1554.507 kb on - strand at 1554.531 kb on + strand at 1554.542 kb on - strand at 1554.542 kb on - strand at 1554.542 kb on - strand at 1554.542 kb on - strand at 1554.565 kb on - strand at 1554.569 kb on + strand at 1554.607 kb on - strand at 1554.608 kb on + strand at 1554.659 kb on - strand at 1554.661 kb on - strand, within MMJJ_RS08450 at 1554.662 kb on + strand, within MMJJ_RS08450 at 1554.684 kb on + strand, within MMJJ_RS08450 at 1554.769 kb on - strand, within MMJJ_RS08450 at 1554.770 kb on + strand, within MMJJ_RS08450 at 1554.805 kb on - strand, within MMJJ_RS08450 at 1554.828 kb on + strand, within MMJJ_RS08450 at 1554.829 kb on - strand, within MMJJ_RS08450 at 1554.829 kb on - strand, within MMJJ_RS08450 at 1554.830 kb on + strand, within MMJJ_RS08450 at 1554.830 kb on + strand, within MMJJ_RS08450 at 1554.830 kb on + strand, within MMJJ_RS08450 at 1554.876 kb on + strand, within MMJJ_RS08450 at 1554.904 kb on - strand, within MMJJ_RS08450 at 1554.926 kb on - strand, within MMJJ_RS08450 at 1554.935 kb on + strand, within MMJJ_RS08450 at 1554.935 kb on + strand, within MMJJ_RS08450 at 1554.959 kb on + strand, within MMJJ_RS08450 at 1554.981 kb on + strand, within MMJJ_RS08450 at 1554.981 kb on + strand, within MMJJ_RS08450 at 1554.987 kb on + strand, within MMJJ_RS08450 at 1555.008 kb on + strand, within MMJJ_RS08450 at 1555.088 kb on + strand, within MMJJ_RS08450 at 1555.093 kb on - strand, within MMJJ_RS08450 at 1555.162 kb on - strand, within MMJJ_RS08450 at 1555.162 kb on - strand, within MMJJ_RS08450 at 1555.162 kb on - strand, within MMJJ_RS08450 at 1555.162 kb on - strand, within MMJJ_RS08450 at 1555.163 kb on + strand, within MMJJ_RS08450 at 1555.178 kb on + strand, within MMJJ_RS08450 at 1555.234 kb on - strand, within MMJJ_RS08450 at 1555.242 kb on - strand, within MMJJ_RS08450 at 1555.261 kb on - strand at 1555.293 kb on - strand at 1555.319 kb on + strand at 1555.443 kb on - strand, within MMJJ_RS08455 at 1555.608 kb on - strand, within MMJJ_RS08455 at 1555.608 kb on - strand, within MMJJ_RS08455 at 1555.608 kb on - strand, within MMJJ_RS08455 at 1555.609 kb on + strand, within MMJJ_RS08455 at 1555.609 kb on + strand, within MMJJ_RS08455 at 1555.635 kb on - strand, within MMJJ_RS08455 at 1555.636 kb on + strand, within MMJJ_RS08455 at 1555.636 kb on + strand, within MMJJ_RS08455 at 1555.677 kb on - strand, within MMJJ_RS08455 at 1555.679 kb on - strand, within MMJJ_RS08455 at 1555.728 kb on - strand at 1555.735 kb on - strand at 1555.739 kb on - strand at 1555.824 kb on + strand, within MMJJ_RS08460 at 1555.849 kb on - strand, within MMJJ_RS08460 at 1555.958 kb on + strand, within MMJJ_RS08460 at 1555.976 kb on - strand, within MMJJ_RS08460 at 1555.980 kb on - strand, within MMJJ_RS08460 at 1555.981 kb on + strand, within MMJJ_RS08460 at 1556.013 kb on - strand, within MMJJ_RS08460 at 1556.014 kb on + strand, within MMJJ_RS08460 at 1556.073 kb on + strand, within MMJJ_RS08460 at 1556.087 kb on + strand at 1556.118 kb on - strand, within MMJJ_RS08460 at 1556.121 kb on - strand, within MMJJ_RS08460 at 1556.122 kb on + strand, within MMJJ_RS08460 at 1556.122 kb on + strand, within MMJJ_RS08460 at 1556.125 kb on - strand, within MMJJ_RS08460 at 1556.136 kb on - strand, within MMJJ_RS08460 at 1556.137 kb on + strand, within MMJJ_RS08460 at 1556.137 kb on + strand, within MMJJ_RS08460 at 1556.137 kb on + strand, within MMJJ_RS08460 at 1556.209 kb on - strand, within MMJJ_RS08460 at 1556.216 kb on - strand, within MMJJ_RS08460 at 1556.216 kb on - strand, within MMJJ_RS08460 at 1556.217 kb on + strand, within MMJJ_RS08460 at 1556.217 kb on + strand, within MMJJ_RS08460 at 1556.218 kb on - strand, within MMJJ_RS08460 at 1556.254 kb on + strand, within MMJJ_RS08460 at 1556.286 kb on - strand, within MMJJ_RS08460 at 1556.297 kb on - strand, within MMJJ_RS08460
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 1,553,642 + +0.7 1,553,703 + +0.1 1,553,725 + MMJJ_RS08445 0.10 +2.1 1,553,732 - MMJJ_RS08445 0.11 -1.8 1,553,732 - MMJJ_RS08445 0.11 -1.9 1,553,733 + MMJJ_RS08445 0.11 -0.3 1,553,750 + MMJJ_RS08445 0.13 +0.9 1,553,764 + MMJJ_RS08445 0.15 +1.3 1,553,804 + MMJJ_RS08445 0.20 +2.0 1,553,818 + MMJJ_RS08445 0.22 -1.6 1,553,929 + MMJJ_RS08445 0.36 -0.0 1,553,948 + MMJJ_RS08445 0.39 -0.1 1,554,097 - MMJJ_RS08445 0.57 -0.6 1,554,097 - MMJJ_RS08445 0.57 -3.0 1,554,127 - MMJJ_RS08445 0.61 -1.7 1,554,175 + MMJJ_RS08445 0.67 +0.1 1,554,210 - MMJJ_RS08445 0.72 +0.3 1,554,210 - MMJJ_RS08445 0.72 +2.4 1,554,210 - MMJJ_RS08445 0.72 +2.4 1,554,211 + MMJJ_RS08445 0.72 +1.2 1,554,219 + MMJJ_RS08445 0.73 -3.5 1,554,455 - +0.4 1,554,455 - +0.4 1,554,465 + -4.0 1,554,472 - -2.1 1,554,472 - -2.4 1,554,473 + +0.7 1,554,476 - +0.5 1,554,477 + +1.7 1,554,500 + -0.5 1,554,500 + -0.2 1,554,504 + +2.2 1,554,504 + +2.1 1,554,507 - +0.8 1,554,531 + -3.3 1,554,542 - -0.4 1,554,542 - -3.7 1,554,542 - -1.8 1,554,542 - -0.1 1,554,565 - -2.2 1,554,569 + -3.2 1,554,607 - -3.9 1,554,608 + -0.6 1,554,659 - -3.4 1,554,661 - MMJJ_RS08450 0.10 -2.4 1,554,662 + MMJJ_RS08450 0.10 -1.8 1,554,684 + MMJJ_RS08450 0.13 -3.6 1,554,769 - MMJJ_RS08450 0.25 -3.1 1,554,770 + MMJJ_RS08450 0.25 -2.6 1,554,805 - MMJJ_RS08450 0.29 -3.2 1,554,828 + MMJJ_RS08450 0.32 -4.0 1,554,829 - MMJJ_RS08450 0.33 -2.5 1,554,829 - MMJJ_RS08450 0.33 -0.8 1,554,830 + MMJJ_RS08450 0.33 -3.1 1,554,830 + MMJJ_RS08450 0.33 -2.6 1,554,830 + MMJJ_RS08450 0.33 -2.9 1,554,876 + MMJJ_RS08450 0.39 -0.6 1,554,904 - MMJJ_RS08450 0.43 -2.3 1,554,926 - MMJJ_RS08450 0.45 -3.5 1,554,935 + MMJJ_RS08450 0.47 -3.2 1,554,935 + MMJJ_RS08450 0.47 -2.3 1,554,959 + MMJJ_RS08450 0.50 -3.5 1,554,981 + MMJJ_RS08450 0.53 -4.7 1,554,981 + MMJJ_RS08450 0.53 -2.0 1,554,987 + MMJJ_RS08450 0.54 -4.5 1,555,008 + MMJJ_RS08450 0.56 -3.2 1,555,088 + MMJJ_RS08450 0.67 -3.4 1,555,093 - MMJJ_RS08450 0.68 -2.4 1,555,162 - MMJJ_RS08450 0.77 -0.6 1,555,162 - MMJJ_RS08450 0.77 -2.4 1,555,162 - MMJJ_RS08450 0.77 -4.0 1,555,162 - MMJJ_RS08450 0.77 -3.2 1,555,163 + MMJJ_RS08450 0.77 -3.5 1,555,178 + MMJJ_RS08450 0.79 -4.4 1,555,234 - MMJJ_RS08450 0.87 -3.7 1,555,242 - MMJJ_RS08450 0.88 -3.5 1,555,261 - -2.4 1,555,293 - -2.3 1,555,319 + -4.0 1,555,443 - MMJJ_RS08455 0.24 +0.6 1,555,608 - MMJJ_RS08455 0.68 +0.7 1,555,608 - MMJJ_RS08455 0.68 -0.6 1,555,608 - MMJJ_RS08455 0.68 +0.4 1,555,609 + MMJJ_RS08455 0.68 +0.2 1,555,609 + MMJJ_RS08455 0.68 -0.5 1,555,635 - MMJJ_RS08455 0.75 -1.7 1,555,636 + MMJJ_RS08455 0.75 +0.9 1,555,636 + MMJJ_RS08455 0.75 +0.2 1,555,677 - MMJJ_RS08455 0.86 -3.4 1,555,679 - MMJJ_RS08455 0.86 -3.9 1,555,728 - -3.8 1,555,735 - +2.7 1,555,739 - -0.8 1,555,824 + MMJJ_RS08460 0.10 -0.6 1,555,849 - MMJJ_RS08460 0.14 +1.0 1,555,958 + MMJJ_RS08460 0.29 -1.8 1,555,976 - MMJJ_RS08460 0.32 -0.7 1,555,980 - MMJJ_RS08460 0.32 +1.0 1,555,981 + MMJJ_RS08460 0.32 +0.4 1,556,013 - MMJJ_RS08460 0.37 -2.4 1,556,014 + MMJJ_RS08460 0.37 -2.3 1,556,073 + MMJJ_RS08460 0.45 -3.0 1,556,087 + +1.4 1,556,118 - MMJJ_RS08460 0.51 +1.3 1,556,121 - MMJJ_RS08460 0.52 -0.5 1,556,122 + MMJJ_RS08460 0.52 -2.4 1,556,122 + MMJJ_RS08460 0.52 -1.4 1,556,125 - MMJJ_RS08460 0.52 -0.8 1,556,136 - MMJJ_RS08460 0.54 -0.1 1,556,137 + MMJJ_RS08460 0.54 -0.7 1,556,137 + MMJJ_RS08460 0.54 -0.2 1,556,137 + MMJJ_RS08460 0.54 +0.4 1,556,209 - MMJJ_RS08460 0.64 +0.7 1,556,216 - MMJJ_RS08460 0.65 -1.5 1,556,216 - MMJJ_RS08460 0.65 -0.0 1,556,217 + MMJJ_RS08460 0.65 -0.0 1,556,217 + MMJJ_RS08460 0.65 +0.2 1,556,218 - MMJJ_RS08460 0.66 +0.4 1,556,254 + MMJJ_RS08460 0.71 -3.4 1,556,286 - MMJJ_RS08460 0.75 +0.3 1,556,297 - MMJJ_RS08460 0.77 -2.5
Or see this region's nucleotide sequence