Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08210 and MMJJ_RS08215 are separated by 626 nucleotides MMJJ_RS08215 and MMJJ_RS08220 are separated by 120 nucleotides
MMJJ_RS08210: MMJJ_RS08210 - hypothetical protein, at 1,507,482 to 1,511,972
_RS08210
MMJJ_RS08215: MMJJ_RS08215 - radical SAM protein, at 1,512,599 to 1,513,714
_RS08215
MMJJ_RS08220: MMJJ_RS08220 - methenyltetrahydromethanopterin cyclohydrolase, at 1,513,835 to 1,514,806
_RS08220
Position (kb)
1512
1513
1514 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1511.684 kb on - strand at 1511.686 kb on - strand at 1511.686 kb on - strand at 1511.686 kb on - strand at 1511.687 kb on + strand at 1511.687 kb on + strand at 1511.691 kb on - strand at 1511.691 kb on - strand at 1511.692 kb on + strand at 1511.697 kb on - strand at 1511.697 kb on - strand at 1511.697 kb on - strand at 1511.697 kb on - strand at 1511.728 kb on - strand at 1511.729 kb on + strand at 1511.729 kb on + strand at 1511.737 kb on + strand at 1511.795 kb on - strand at 1511.795 kb on - strand at 1511.795 kb on - strand at 1511.796 kb on + strand at 1511.796 kb on + strand at 1511.807 kb on - strand at 1511.807 kb on - strand at 1511.816 kb on - strand at 1511.817 kb on + strand at 1511.831 kb on - strand at 1511.838 kb on - strand at 1511.838 kb on - strand at 1511.838 kb on - strand at 1511.838 kb on - strand at 1511.839 kb on + strand at 1511.839 kb on + strand at 1511.839 kb on + strand at 1511.839 kb on + strand at 1511.839 kb on + strand at 1511.840 kb on - strand at 1511.840 kb on - strand at 1511.841 kb on + strand at 1511.845 kb on + strand at 1511.845 kb on + strand at 1511.845 kb on + strand at 1511.864 kb on - strand at 1511.864 kb on - strand at 1511.865 kb on + strand at 1511.865 kb on + strand at 1511.865 kb on + strand at 1511.865 kb on + strand at 1511.881 kb on + strand at 1511.881 kb on + strand at 1511.888 kb on - strand at 1511.888 kb on - strand at 1511.888 kb on - strand at 1511.889 kb on + strand at 1511.892 kb on + strand at 1511.937 kb on - strand at 1512.006 kb on + strand at 1512.019 kb on + strand at 1512.049 kb on + strand at 1512.084 kb on + strand at 1512.133 kb on + strand at 1512.172 kb on - strand at 1512.186 kb on + strand at 1512.426 kb on - strand at 1512.440 kb on - strand at 1512.480 kb on + strand at 1512.568 kb on + strand at 1512.691 kb on - strand at 1512.692 kb on + strand at 1512.694 kb on + strand at 1512.771 kb on + strand, within MMJJ_RS08215 at 1512.771 kb on + strand, within MMJJ_RS08215 at 1512.825 kb on + strand, within MMJJ_RS08215 at 1512.850 kb on - strand, within MMJJ_RS08215 at 1512.860 kb on + strand, within MMJJ_RS08215 at 1512.870 kb on + strand, within MMJJ_RS08215 at 1512.991 kb on - strand, within MMJJ_RS08215 at 1512.991 kb on - strand, within MMJJ_RS08215 at 1512.992 kb on + strand, within MMJJ_RS08215 at 1512.992 kb on + strand, within MMJJ_RS08215 at 1513.001 kb on + strand, within MMJJ_RS08215 at 1513.017 kb on - strand, within MMJJ_RS08215 at 1513.018 kb on + strand, within MMJJ_RS08215 at 1513.058 kb on + strand, within MMJJ_RS08215 at 1513.098 kb on - strand, within MMJJ_RS08215 at 1513.098 kb on - strand, within MMJJ_RS08215 at 1513.098 kb on - strand, within MMJJ_RS08215 at 1513.233 kb on - strand, within MMJJ_RS08215 at 1513.274 kb on + strand, within MMJJ_RS08215 at 1513.298 kb on - strand, within MMJJ_RS08215 at 1513.298 kb on - strand, within MMJJ_RS08215 at 1513.299 kb on + strand, within MMJJ_RS08215 at 1513.344 kb on + strand, within MMJJ_RS08215 at 1513.386 kb on - strand, within MMJJ_RS08215 at 1513.434 kb on + strand, within MMJJ_RS08215 at 1513.437 kb on - strand, within MMJJ_RS08215 at 1513.437 kb on - strand, within MMJJ_RS08215 at 1513.460 kb on - strand, within MMJJ_RS08215 at 1513.481 kb on - strand, within MMJJ_RS08215 at 1513.495 kb on - strand, within MMJJ_RS08215 at 1513.509 kb on - strand, within MMJJ_RS08215 at 1513.509 kb on - strand, within MMJJ_RS08215 at 1513.509 kb on - strand, within MMJJ_RS08215 at 1513.602 kb on - strand, within MMJJ_RS08215 at 1513.605 kb on - strand at 1513.630 kb on - strand at 1513.631 kb on + strand at 1513.633 kb on - strand at 1513.633 kb on - strand at 1513.635 kb on - strand at 1513.635 kb on - strand at 1513.658 kb on - strand at 1513.658 kb on - strand at 1513.665 kb on - strand at 1513.771 kb on - strand at 1513.774 kb on - strand at 1513.775 kb on + strand at 1513.787 kb on + strand at 1513.803 kb on - strand at 1513.807 kb on - strand at 1513.807 kb on - strand at 1513.808 kb on + strand at 1513.808 kb on + strand at 1513.808 kb on + strand at 1513.809 kb on - strand at 1513.813 kb on - strand at 1513.814 kb on + strand at 1513.815 kb on - strand at 1513.820 kb on + strand at 1513.820 kb on + strand at 1513.822 kb on + strand at 1513.826 kb on - strand at 1513.826 kb on - strand at 1513.826 kb on - strand at 1513.827 kb on + strand at 1513.827 kb on + strand at 1513.827 kb on + strand at 1513.831 kb on - strand at 1513.837 kb on + strand at 1513.838 kb on - strand at 1513.839 kb on + strand at 1513.868 kb on - strand at 1513.869 kb on + strand at 1513.891 kb on + strand at 1513.991 kb on - strand, within MMJJ_RS08220 at 1514.681 kb on - strand at 1514.708 kb on + strand, within MMJJ_RS08220
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 1,511,684 - +1.0 1,511,686 - -4.2 1,511,686 - +0.0 1,511,686 - +1.1 1,511,687 + +1.5 1,511,687 + +0.7 1,511,691 - -1.7 1,511,691 - +1.2 1,511,692 + -1.8 1,511,697 - +0.9 1,511,697 - +0.9 1,511,697 - -2.2 1,511,697 - -0.3 1,511,728 - -0.7 1,511,729 + +2.4 1,511,729 + -3.0 1,511,737 + -3.2 1,511,795 - +0.5 1,511,795 - +1.0 1,511,795 - +0.9 1,511,796 + +0.2 1,511,796 + +0.6 1,511,807 - -2.2 1,511,807 - +1.5 1,511,816 - -0.4 1,511,817 + +2.2 1,511,831 - -2.3 1,511,838 - +0.6 1,511,838 - +0.9 1,511,838 - +0.5 1,511,838 - +0.8 1,511,839 + -2.2 1,511,839 + +0.3 1,511,839 + -0.2 1,511,839 + -1.6 1,511,839 + +0.7 1,511,840 - -2.1 1,511,840 - -1.6 1,511,841 + -2.2 1,511,845 + -0.7 1,511,845 + -0.8 1,511,845 + -1.0 1,511,864 - +1.3 1,511,864 - -0.5 1,511,865 + -4.0 1,511,865 + +0.1 1,511,865 + -0.2 1,511,865 + +1.3 1,511,881 + +0.7 1,511,881 + -0.3 1,511,888 - +0.7 1,511,888 - +2.4 1,511,888 - +0.8 1,511,889 + +0.0 1,511,892 + -2.3 1,511,937 - -1.0 1,512,006 + -1.4 1,512,019 + -0.4 1,512,049 + +0.7 1,512,084 + +0.7 1,512,133 + -2.4 1,512,172 - +1.4 1,512,186 + +0.3 1,512,426 - -0.5 1,512,440 - -0.3 1,512,480 + +2.0 1,512,568 + -0.7 1,512,691 - -2.2 1,512,692 + -1.4 1,512,694 + -0.4 1,512,771 + MMJJ_RS08215 0.15 -1.7 1,512,771 + MMJJ_RS08215 0.15 -0.7 1,512,825 + MMJJ_RS08215 0.20 +0.2 1,512,850 - MMJJ_RS08215 0.22 -3.2 1,512,860 + MMJJ_RS08215 0.23 -1.2 1,512,870 + MMJJ_RS08215 0.24 -2.4 1,512,991 - MMJJ_RS08215 0.35 -0.5 1,512,991 - MMJJ_RS08215 0.35 -0.6 1,512,992 + MMJJ_RS08215 0.35 +0.3 1,512,992 + MMJJ_RS08215 0.35 -2.1 1,513,001 + MMJJ_RS08215 0.36 -2.1 1,513,017 - MMJJ_RS08215 0.37 -1.3 1,513,018 + MMJJ_RS08215 0.38 -2.8 1,513,058 + MMJJ_RS08215 0.41 -0.1 1,513,098 - MMJJ_RS08215 0.45 +0.1 1,513,098 - MMJJ_RS08215 0.45 -0.1 1,513,098 - MMJJ_RS08215 0.45 -0.1 1,513,233 - MMJJ_RS08215 0.57 -2.1 1,513,274 + MMJJ_RS08215 0.60 -1.2 1,513,298 - MMJJ_RS08215 0.63 +0.9 1,513,298 - MMJJ_RS08215 0.63 -0.5 1,513,299 + MMJJ_RS08215 0.63 -2.1 1,513,344 + MMJJ_RS08215 0.67 -3.2 1,513,386 - MMJJ_RS08215 0.71 -1.5 1,513,434 + MMJJ_RS08215 0.75 -0.5 1,513,437 - MMJJ_RS08215 0.75 -4.3 1,513,437 - MMJJ_RS08215 0.75 -2.3 1,513,460 - MMJJ_RS08215 0.77 +0.2 1,513,481 - MMJJ_RS08215 0.79 -1.6 1,513,495 - MMJJ_RS08215 0.80 -2.0 1,513,509 - MMJJ_RS08215 0.82 -0.5 1,513,509 - MMJJ_RS08215 0.82 -2.5 1,513,509 - MMJJ_RS08215 0.82 -3.1 1,513,602 - MMJJ_RS08215 0.90 -3.9 1,513,605 - -2.5 1,513,630 - +0.6 1,513,631 + -0.4 1,513,633 - -2.4 1,513,633 - -3.1 1,513,635 - -4.1 1,513,635 - +0.1 1,513,658 - -2.4 1,513,658 - -1.2 1,513,665 - -2.1 1,513,771 - +1.0 1,513,774 - -1.5 1,513,775 + -1.6 1,513,787 + +1.2 1,513,803 - -1.6 1,513,807 - -3.2 1,513,807 - -2.7 1,513,808 + -0.6 1,513,808 + -3.8 1,513,808 + -2.9 1,513,809 - -1.2 1,513,813 - -1.5 1,513,814 + -1.4 1,513,815 - -1.7 1,513,820 + -3.7 1,513,820 + -2.1 1,513,822 + -0.4 1,513,826 - -2.2 1,513,826 - -2.3 1,513,826 - -1.0 1,513,827 + -3.0 1,513,827 + -0.7 1,513,827 + -2.3 1,513,831 - -2.2 1,513,837 + -2.8 1,513,838 - -3.4 1,513,839 + -3.3 1,513,868 - -3.1 1,513,869 + -1.2 1,513,891 + -1.3 1,513,991 - MMJJ_RS08220 0.16 -1.6 1,514,681 - +0.4 1,514,708 + MMJJ_RS08220 0.90 -1.8
Or see this region's nucleotide sequence