Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06655 and MMJJ_RS06660 are separated by 6 nucleotides MMJJ_RS06660 and MMJJ_RS06665 overlap by 4 nucleotides MMJJ_RS06665 and MMJJ_RS06670 are separated by 24 nucleotides
MMJJ_RS06655: MMJJ_RS06655 - transcriptional regulator, at 1,227,451 to 1,227,963
_RS06655
MMJJ_RS06660: MMJJ_RS06660 - DUF1188 domain-containing protein, at 1,227,970 to 1,228,752
_RS06660
MMJJ_RS06665: MMJJ_RS06665 - DUF3236 domain-containing protein, at 1,228,749 to 1,229,228
_RS06665
MMJJ_RS06670: MMJJ_RS06670 - phenylalanine--tRNA ligase subunit alpha, at 1,229,253 to 1,230,758
_RS06670
Position (kb)
1228
1229
1230 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1227.799 kb on + strand, within MMJJ_RS06655 at 1227.812 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.814 kb on - strand, within MMJJ_RS06655 at 1227.858 kb on + strand, within MMJJ_RS06655 at 1227.861 kb on + strand, within MMJJ_RS06655 at 1227.864 kb on - strand, within MMJJ_RS06655 at 1227.884 kb on - strand, within MMJJ_RS06655 at 1227.885 kb on + strand, within MMJJ_RS06655 at 1227.891 kb on + strand, within MMJJ_RS06655 at 1227.891 kb on + strand, within MMJJ_RS06655 at 1227.907 kb on + strand, within MMJJ_RS06655 at 1227.972 kb on + strand at 1227.994 kb on - strand at 1227.994 kb on - strand at 1227.995 kb on + strand at 1227.995 kb on + strand at 1228.005 kb on - strand at 1228.019 kb on + strand at 1228.064 kb on + strand, within MMJJ_RS06660 at 1228.133 kb on - strand, within MMJJ_RS06660 at 1228.134 kb on + strand, within MMJJ_RS06660 at 1228.164 kb on - strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.169 kb on + strand, within MMJJ_RS06660 at 1228.201 kb on - strand, within MMJJ_RS06660 at 1228.312 kb on - strand, within MMJJ_RS06660 at 1228.410 kb on - strand, within MMJJ_RS06660 at 1228.510 kb on - strand, within MMJJ_RS06660 at 1228.510 kb on - strand, within MMJJ_RS06660 at 1228.516 kb on + strand, within MMJJ_RS06660 at 1228.522 kb on - strand, within MMJJ_RS06660 at 1228.541 kb on + strand, within MMJJ_RS06660 at 1228.549 kb on + strand, within MMJJ_RS06660 at 1228.567 kb on - strand, within MMJJ_RS06660 at 1228.587 kb on + strand, within MMJJ_RS06660 at 1228.601 kb on - strand, within MMJJ_RS06660 at 1228.626 kb on - strand, within MMJJ_RS06660 at 1228.642 kb on - strand, within MMJJ_RS06660 at 1228.643 kb on + strand, within MMJJ_RS06660 at 1228.643 kb on + strand, within MMJJ_RS06660 at 1228.649 kb on - strand, within MMJJ_RS06660 at 1228.766 kb on - strand at 1228.767 kb on + strand at 1228.797 kb on - strand, within MMJJ_RS06665 at 1228.932 kb on - strand, within MMJJ_RS06665 at 1228.933 kb on + strand, within MMJJ_RS06665 at 1228.981 kb on + strand, within MMJJ_RS06665 at 1228.998 kb on - strand, within MMJJ_RS06665 at 1229.035 kb on + strand, within MMJJ_RS06665 at 1229.036 kb on - strand, within MMJJ_RS06665 at 1229.036 kb on - strand, within MMJJ_RS06665 at 1229.060 kb on - strand, within MMJJ_RS06665 at 1229.109 kb on - strand, within MMJJ_RS06665 at 1229.157 kb on - strand, within MMJJ_RS06665 at 1229.177 kb on - strand, within MMJJ_RS06665 at 1229.178 kb on + strand, within MMJJ_RS06665 at 1229.197 kb on - strand at 1229.213 kb on - strand at 1229.254 kb on - strand at 1229.501 kb on + strand at 1229.655 kb on + strand at 1229.994 kb on - strand, within MMJJ_RS06670
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 1,227,799 + MMJJ_RS06655 0.68 -3.8 1,227,812 - MMJJ_RS06655 0.70 +1.1 1,227,814 - MMJJ_RS06655 0.71 -0.1 1,227,814 - MMJJ_RS06655 0.71 -3.2 1,227,814 - MMJJ_RS06655 0.71 -1.3 1,227,858 + MMJJ_RS06655 0.79 -0.4 1,227,861 + MMJJ_RS06655 0.80 -1.1 1,227,864 - MMJJ_RS06655 0.81 -3.3 1,227,884 - MMJJ_RS06655 0.84 -0.9 1,227,885 + MMJJ_RS06655 0.85 -4.2 1,227,891 + MMJJ_RS06655 0.86 -0.4 1,227,891 + MMJJ_RS06655 0.86 +0.4 1,227,907 + MMJJ_RS06655 0.89 -1.5 1,227,972 + -0.0 1,227,994 - -3.2 1,227,994 - -1.4 1,227,995 + -3.7 1,227,995 + -2.2 1,228,005 - -2.6 1,228,019 + -4.5 1,228,064 + MMJJ_RS06660 0.12 -2.2 1,228,133 - MMJJ_RS06660 0.21 -2.5 1,228,134 + MMJJ_RS06660 0.21 -3.2 1,228,164 - MMJJ_RS06660 0.25 -1.7 1,228,169 + MMJJ_RS06660 0.25 -1.5 1,228,169 + MMJJ_RS06660 0.25 -2.2 1,228,169 + MMJJ_RS06660 0.25 -0.3 1,228,201 - MMJJ_RS06660 0.30 -2.2 1,228,312 - MMJJ_RS06660 0.44 -2.4 1,228,410 - MMJJ_RS06660 0.56 -3.7 1,228,510 - MMJJ_RS06660 0.69 -3.0 1,228,510 - MMJJ_RS06660 0.69 -3.3 1,228,516 + MMJJ_RS06660 0.70 -2.4 1,228,522 - MMJJ_RS06660 0.70 -3.0 1,228,541 + MMJJ_RS06660 0.73 -3.9 1,228,549 + MMJJ_RS06660 0.74 +0.5 1,228,567 - MMJJ_RS06660 0.76 -4.5 1,228,587 + MMJJ_RS06660 0.79 -1.5 1,228,601 - MMJJ_RS06660 0.81 -4.1 1,228,626 - MMJJ_RS06660 0.84 -3.8 1,228,642 - MMJJ_RS06660 0.86 -2.9 1,228,643 + MMJJ_RS06660 0.86 -3.9 1,228,643 + MMJJ_RS06660 0.86 -2.0 1,228,649 - MMJJ_RS06660 0.87 -3.1 1,228,766 - -1.8 1,228,767 + -4.0 1,228,797 - MMJJ_RS06665 0.10 -2.4 1,228,932 - MMJJ_RS06665 0.38 -2.3 1,228,933 + MMJJ_RS06665 0.38 -3.6 1,228,981 + MMJJ_RS06665 0.48 -3.7 1,228,998 - MMJJ_RS06665 0.52 -1.8 1,229,035 + MMJJ_RS06665 0.60 +1.9 1,229,036 - MMJJ_RS06665 0.60 -1.8 1,229,036 - MMJJ_RS06665 0.60 -2.0 1,229,060 - MMJJ_RS06665 0.65 -2.1 1,229,109 - MMJJ_RS06665 0.75 -3.4 1,229,157 - MMJJ_RS06665 0.85 +0.9 1,229,177 - MMJJ_RS06665 0.89 -3.5 1,229,178 + MMJJ_RS06665 0.89 -0.8 1,229,197 - -3.4 1,229,213 - -1.7 1,229,254 - -0.4 1,229,501 + -0.1 1,229,655 + -0.5 1,229,994 - MMJJ_RS06670 0.49 -1.3
Or see this region's nucleotide sequence