Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04570 and MMJJ_RS04575 are separated by 60 nucleotides MMJJ_RS04575 and MMJJ_RS04580 are separated by 18 nucleotides MMJJ_RS04580 and MMJJ_RS04585 are separated by 151 nucleotides
MMJJ_RS04570: MMJJ_RS04570 - TRC40/GET3/ArsA family transport-energizing ATPase, at 852,519 to 853,556
_RS04570
MMJJ_RS04575: MMJJ_RS04575 - DUF116 domain-containing protein, at 853,617 to 854,261
_RS04575
MMJJ_RS04580: MMJJ_RS04580 - 2-phosphosulfolactate phosphatase, at 854,280 to 855,026
_RS04580
MMJJ_RS04585: MMJJ_RS04585 - phenylacetate--CoA ligase, at 855,178 to 856,479
_RS04585
Position (kb) 854
855
856 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 853.588 kb on + strand at 853.647 kb on - strand at 853.794 kb on + strand, within MMJJ_RS04575 at 853.833 kb on - strand, within MMJJ_RS04575 at 853.834 kb on + strand, within MMJJ_RS04575 at 853.834 kb on + strand, within MMJJ_RS04575 at 853.837 kb on - strand, within MMJJ_RS04575 at 853.838 kb on + strand, within MMJJ_RS04575 at 853.845 kb on + strand, within MMJJ_RS04575 at 853.846 kb on - strand, within MMJJ_RS04575 at 853.944 kb on - strand, within MMJJ_RS04575 at 853.953 kb on - strand, within MMJJ_RS04575 at 853.971 kb on - strand, within MMJJ_RS04575 at 854.048 kb on - strand, within MMJJ_RS04575 at 854.082 kb on - strand, within MMJJ_RS04575 at 854.083 kb on + strand, within MMJJ_RS04575 at 854.083 kb on + strand, within MMJJ_RS04575 at 854.092 kb on - strand, within MMJJ_RS04575 at 854.092 kb on - strand, within MMJJ_RS04575 at 854.093 kb on + strand, within MMJJ_RS04575 at 854.093 kb on + strand, within MMJJ_RS04575 at 854.105 kb on - strand, within MMJJ_RS04575 at 854.105 kb on - strand, within MMJJ_RS04575 at 854.105 kb on - strand, within MMJJ_RS04575 at 854.106 kb on + strand, within MMJJ_RS04575 at 854.109 kb on + strand, within MMJJ_RS04575 at 854.110 kb on - strand, within MMJJ_RS04575 at 854.110 kb on - strand, within MMJJ_RS04575 at 854.110 kb on - strand, within MMJJ_RS04575 at 854.111 kb on + strand, within MMJJ_RS04575 at 854.111 kb on + strand at 854.111 kb on + strand, within MMJJ_RS04575 at 854.111 kb on + strand, within MMJJ_RS04575 at 854.111 kb on + strand, within MMJJ_RS04575 at 854.118 kb on - strand, within MMJJ_RS04575 at 854.119 kb on + strand, within MMJJ_RS04575 at 854.124 kb on + strand, within MMJJ_RS04575 at 854.149 kb on + strand, within MMJJ_RS04575 at 854.150 kb on - strand, within MMJJ_RS04575 at 854.156 kb on + strand, within MMJJ_RS04575 at 854.178 kb on - strand, within MMJJ_RS04575 at 854.179 kb on + strand, within MMJJ_RS04575 at 854.196 kb on - strand, within MMJJ_RS04575 at 854.206 kb on + strand at 854.208 kb on - strand at 854.226 kb on - strand at 854.227 kb on + strand at 854.227 kb on + strand at 854.315 kb on - strand at 854.350 kb on + strand at 854.374 kb on - strand, within MMJJ_RS04580 at 854.396 kb on - strand, within MMJJ_RS04580 at 854.397 kb on + strand, within MMJJ_RS04580 at 854.402 kb on + strand, within MMJJ_RS04580 at 854.422 kb on - strand, within MMJJ_RS04580 at 854.422 kb on - strand, within MMJJ_RS04580 at 854.422 kb on - strand, within MMJJ_RS04580 at 854.423 kb on + strand, within MMJJ_RS04580 at 854.424 kb on - strand, within MMJJ_RS04580 at 854.424 kb on - strand, within MMJJ_RS04580 at 854.424 kb on - strand, within MMJJ_RS04580 at 854.424 kb on - strand, within MMJJ_RS04580 at 854.424 kb on - strand, within MMJJ_RS04580 at 854.424 kb on - strand, within MMJJ_RS04580 at 854.424 kb on - strand, within MMJJ_RS04580 at 854.424 kb on - strand, within MMJJ_RS04580 at 854.425 kb on + strand, within MMJJ_RS04580 at 854.425 kb on + strand, within MMJJ_RS04580 at 854.425 kb on + strand, within MMJJ_RS04580 at 854.425 kb on + strand, within MMJJ_RS04580 at 854.425 kb on + strand, within MMJJ_RS04580 at 854.425 kb on + strand, within MMJJ_RS04580 at 854.425 kb on + strand, within MMJJ_RS04580 at 854.425 kb on + strand, within MMJJ_RS04580 at 854.425 kb on + strand, within MMJJ_RS04580 at 854.425 kb on + strand, within MMJJ_RS04580 at 854.426 kb on - strand, within MMJJ_RS04580 at 854.426 kb on - strand, within MMJJ_RS04580 at 854.426 kb on - strand, within MMJJ_RS04580 at 854.426 kb on - strand, within MMJJ_RS04580 at 854.426 kb on - strand, within MMJJ_RS04580 at 854.426 kb on - strand, within MMJJ_RS04580 at 854.426 kb on - strand, within MMJJ_RS04580 at 854.426 kb on - strand, within MMJJ_RS04580 at 854.426 kb on - strand, within MMJJ_RS04580 at 854.427 kb on + strand, within MMJJ_RS04580 at 854.427 kb on + strand at 854.427 kb on + strand, within MMJJ_RS04580 at 854.427 kb on + strand, within MMJJ_RS04580 at 854.427 kb on + strand, within MMJJ_RS04580 at 854.427 kb on + strand, within MMJJ_RS04580 at 854.427 kb on + strand, within MMJJ_RS04580 at 854.427 kb on + strand, within MMJJ_RS04580 at 854.427 kb on + strand, within MMJJ_RS04580 at 854.427 kb on + strand, within MMJJ_RS04580 at 854.427 kb on + strand, within MMJJ_RS04580 at 854.427 kb on + strand, within MMJJ_RS04580 at 854.429 kb on + strand, within MMJJ_RS04580 at 854.429 kb on + strand, within MMJJ_RS04580 at 854.429 kb on + strand, within MMJJ_RS04580 at 854.436 kb on + strand, within MMJJ_RS04580 at 854.436 kb on + strand, within MMJJ_RS04580 at 854.436 kb on + strand, within MMJJ_RS04580 at 854.457 kb on + strand, within MMJJ_RS04580 at 854.458 kb on - strand, within MMJJ_RS04580 at 854.458 kb on - strand, within MMJJ_RS04580 at 854.459 kb on + strand, within MMJJ_RS04580 at 854.537 kb on + strand, within MMJJ_RS04580 at 854.543 kb on + strand, within MMJJ_RS04580 at 854.586 kb on + strand, within MMJJ_RS04580 at 854.642 kb on - strand, within MMJJ_RS04580 at 854.643 kb on + strand, within MMJJ_RS04580 at 854.648 kb on + strand, within MMJJ_RS04580 at 854.661 kb on + strand, within MMJJ_RS04580 at 854.691 kb on - strand, within MMJJ_RS04580 at 854.722 kb on - strand, within MMJJ_RS04580 at 854.797 kb on + strand, within MMJJ_RS04580 at 854.893 kb on - strand at 854.894 kb on + strand, within MMJJ_RS04580 at 854.935 kb on + strand, within MMJJ_RS04580 at 854.958 kb on + strand at 854.958 kb on + strand at 854.958 kb on + strand at 855.030 kb on + strand at 855.039 kb on - strand at 855.039 kb on - strand at 855.041 kb on - strand at 855.041 kb on - strand at 855.042 kb on + strand at 855.042 kb on + strand at 855.042 kb on + strand at 855.042 kb on + strand at 855.042 kb on + strand at 855.042 kb on + strand at 855.043 kb on - strand at 855.043 kb on - strand at 855.043 kb on - strand at 855.044 kb on + strand at 855.044 kb on + strand at 855.044 kb on + strand at 855.048 kb on + strand at 855.060 kb on - strand at 855.061 kb on + strand at 855.061 kb on + strand at 855.061 kb on + strand at 855.065 kb on - strand at 855.065 kb on - strand at 855.065 kb on - strand at 855.066 kb on + strand at 855.066 kb on + strand at 855.074 kb on - strand at 855.075 kb on + strand at 855.075 kb on + strand at 855.076 kb on - strand at 855.077 kb on + strand at 855.077 kb on + strand at 855.086 kb on - strand at 855.107 kb on + strand at 855.131 kb on - strand at 855.131 kb on - strand at 855.131 kb on - strand at 855.139 kb on - strand at 855.175 kb on + strand at 855.190 kb on + strand at 855.228 kb on + strand at 855.243 kb on + strand at 855.243 kb on + strand at 855.275 kb on - strand at 855.283 kb on - strand at 855.283 kb on - strand at 855.284 kb on + strand at 855.327 kb on + strand, within MMJJ_RS04585 at 855.336 kb on + strand, within MMJJ_RS04585 at 855.346 kb on + strand, within MMJJ_RS04585 at 855.366 kb on + strand, within MMJJ_RS04585 at 855.473 kb on - strand, within MMJJ_RS04585 at 855.474 kb on + strand, within MMJJ_RS04585 at 855.528 kb on + strand, within MMJJ_RS04585 at 855.543 kb on + strand, within MMJJ_RS04585 at 855.543 kb on + strand, within MMJJ_RS04585 at 855.605 kb on - strand, within MMJJ_RS04585 at 855.610 kb on - strand, within MMJJ_RS04585 at 855.711 kb on + strand, within MMJJ_RS04585 at 855.794 kb on - strand, within MMJJ_RS04585 at 855.893 kb on - strand, within MMJJ_RS04585 at 855.943 kb on + strand, within MMJJ_RS04585 at 856.025 kb on - strand, within MMJJ_RS04585 at 856.025 kb on - strand, within MMJJ_RS04585
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 853,588 + -1.2 853,647 - -3.1 853,794 + MMJJ_RS04575 0.27 -2.6 853,833 - MMJJ_RS04575 0.33 -2.5 853,834 + MMJJ_RS04575 0.34 -1.4 853,834 + MMJJ_RS04575 0.34 -2.2 853,837 - MMJJ_RS04575 0.34 -2.5 853,838 + MMJJ_RS04575 0.34 -1.4 853,845 + MMJJ_RS04575 0.35 -3.7 853,846 - MMJJ_RS04575 0.36 -2.8 853,944 - MMJJ_RS04575 0.51 -1.4 853,953 - MMJJ_RS04575 0.52 -3.4 853,971 - MMJJ_RS04575 0.55 -0.7 854,048 - MMJJ_RS04575 0.67 -3.3 854,082 - MMJJ_RS04575 0.72 -2.7 854,083 + MMJJ_RS04575 0.72 -1.8 854,083 + MMJJ_RS04575 0.72 -1.8 854,092 - MMJJ_RS04575 0.74 -2.9 854,092 - MMJJ_RS04575 0.74 -2.9 854,093 + MMJJ_RS04575 0.74 -2.4 854,093 + MMJJ_RS04575 0.74 -3.0 854,105 - MMJJ_RS04575 0.76 -2.1 854,105 - MMJJ_RS04575 0.76 -3.3 854,105 - MMJJ_RS04575 0.76 -2.4 854,106 + MMJJ_RS04575 0.76 -3.4 854,109 + MMJJ_RS04575 0.76 -3.3 854,110 - MMJJ_RS04575 0.76 -2.2 854,110 - MMJJ_RS04575 0.76 -0.4 854,110 - MMJJ_RS04575 0.76 -1.2 854,111 + MMJJ_RS04575 0.77 -2.4 854,111 + +0.6 854,111 + MMJJ_RS04575 0.77 -2.8 854,111 + MMJJ_RS04575 0.77 +0.6 854,111 + MMJJ_RS04575 0.77 -2.0 854,118 - MMJJ_RS04575 0.78 -1.2 854,119 + MMJJ_RS04575 0.78 -1.5 854,124 + MMJJ_RS04575 0.79 -2.3 854,149 + MMJJ_RS04575 0.82 -1.0 854,150 - MMJJ_RS04575 0.83 -2.1 854,156 + MMJJ_RS04575 0.84 -2.1 854,178 - MMJJ_RS04575 0.87 -1.2 854,179 + MMJJ_RS04575 0.87 -2.0 854,196 - MMJJ_RS04575 0.90 +2.9 854,206 + -2.8 854,208 - -3.5 854,226 - -1.2 854,227 + -2.8 854,227 + -2.4 854,315 - -4.0 854,350 + -3.3 854,374 - MMJJ_RS04580 0.13 -4.3 854,396 - MMJJ_RS04580 0.16 -2.6 854,397 + MMJJ_RS04580 0.16 -0.4 854,402 + MMJJ_RS04580 0.16 -3.3 854,422 - MMJJ_RS04580 0.19 -2.7 854,422 - MMJJ_RS04580 0.19 -2.2 854,422 - MMJJ_RS04580 0.19 +0.6 854,423 + MMJJ_RS04580 0.19 -4.0 854,424 - MMJJ_RS04580 0.19 -2.5 854,424 - MMJJ_RS04580 0.19 -2.3 854,424 - MMJJ_RS04580 0.19 -3.7 854,424 - MMJJ_RS04580 0.19 -2.7 854,424 - MMJJ_RS04580 0.19 -3.4 854,424 - MMJJ_RS04580 0.19 -3.2 854,424 - MMJJ_RS04580 0.19 -2.9 854,424 - MMJJ_RS04580 0.19 -2.7 854,425 + MMJJ_RS04580 0.19 -2.5 854,425 + MMJJ_RS04580 0.19 -2.1 854,425 + MMJJ_RS04580 0.19 -3.8 854,425 + MMJJ_RS04580 0.19 -2.3 854,425 + MMJJ_RS04580 0.19 -2.0 854,425 + MMJJ_RS04580 0.19 -3.8 854,425 + MMJJ_RS04580 0.19 -2.8 854,425 + MMJJ_RS04580 0.19 -3.3 854,425 + MMJJ_RS04580 0.19 -3.8 854,425 + MMJJ_RS04580 0.19 -1.0 854,426 - MMJJ_RS04580 0.20 -2.3 854,426 - MMJJ_RS04580 0.20 -3.5 854,426 - MMJJ_RS04580 0.20 -1.0 854,426 - MMJJ_RS04580 0.20 -1.2 854,426 - MMJJ_RS04580 0.20 -1.8 854,426 - MMJJ_RS04580 0.20 -1.9 854,426 - MMJJ_RS04580 0.20 -1.8 854,426 - MMJJ_RS04580 0.20 -4.7 854,426 - MMJJ_RS04580 0.20 -3.5 854,427 + MMJJ_RS04580 0.20 -2.0 854,427 + +0.0 854,427 + MMJJ_RS04580 0.20 -2.3 854,427 + MMJJ_RS04580 0.20 -2.9 854,427 + MMJJ_RS04580 0.20 -4.1 854,427 + MMJJ_RS04580 0.20 -4.2 854,427 + MMJJ_RS04580 0.20 -5.3 854,427 + MMJJ_RS04580 0.20 -3.5 854,427 + MMJJ_RS04580 0.20 -3.9 854,427 + MMJJ_RS04580 0.20 -3.7 854,427 + MMJJ_RS04580 0.20 -3.8 854,427 + MMJJ_RS04580 0.20 -3.1 854,429 + MMJJ_RS04580 0.20 +0.3 854,429 + MMJJ_RS04580 0.20 -2.2 854,429 + MMJJ_RS04580 0.20 -1.7 854,436 + MMJJ_RS04580 0.21 -4.3 854,436 + MMJJ_RS04580 0.21 -2.5 854,436 + MMJJ_RS04580 0.21 -3.9 854,457 + MMJJ_RS04580 0.24 -0.6 854,458 - MMJJ_RS04580 0.24 -0.3 854,458 - MMJJ_RS04580 0.24 +0.1 854,459 + MMJJ_RS04580 0.24 +1.6 854,537 + MMJJ_RS04580 0.34 +0.4 854,543 + MMJJ_RS04580 0.35 -1.8 854,586 + MMJJ_RS04580 0.41 -1.4 854,642 - MMJJ_RS04580 0.48 +1.3 854,643 + MMJJ_RS04580 0.49 -0.3 854,648 + MMJJ_RS04580 0.49 -0.9 854,661 + MMJJ_RS04580 0.51 +0.3 854,691 - MMJJ_RS04580 0.55 -1.3 854,722 - MMJJ_RS04580 0.59 +0.3 854,797 + MMJJ_RS04580 0.69 -4.4 854,893 - +0.0 854,894 + MMJJ_RS04580 0.82 -3.3 854,935 + MMJJ_RS04580 0.88 +2.3 854,958 + +0.4 854,958 + -1.0 854,958 + +1.3 855,030 + -1.4 855,039 - -0.8 855,039 - +1.5 855,041 - +0.3 855,041 - +0.2 855,042 + +0.9 855,042 + -3.7 855,042 + +1.1 855,042 + -1.2 855,042 + -0.4 855,042 + -0.6 855,043 - +1.5 855,043 - -1.7 855,043 - -2.2 855,044 + -0.5 855,044 + -1.8 855,044 + -1.3 855,048 + +3.3 855,060 - -2.0 855,061 + -0.8 855,061 + -0.2 855,061 + +1.3 855,065 - +0.0 855,065 - +0.8 855,065 - +0.4 855,066 + +1.2 855,066 + +1.1 855,074 - -1.2 855,075 + +1.3 855,075 + -0.1 855,076 - -3.3 855,077 + +0.7 855,077 + -0.8 855,086 - -0.7 855,107 + +1.9 855,131 - -1.5 855,131 - +2.4 855,131 - -2.8 855,139 - +0.4 855,175 + +0.4 855,190 + +0.2 855,228 + -2.9 855,243 + +1.8 855,243 + +0.7 855,275 - -0.2 855,283 - -1.0 855,283 - +1.5 855,284 + +1.0 855,327 + MMJJ_RS04585 0.11 +0.7 855,336 + MMJJ_RS04585 0.12 -1.0 855,346 + MMJJ_RS04585 0.13 -0.6 855,366 + MMJJ_RS04585 0.14 +0.0 855,473 - MMJJ_RS04585 0.23 +1.5 855,474 + MMJJ_RS04585 0.23 -2.5 855,528 + MMJJ_RS04585 0.27 -4.3 855,543 + MMJJ_RS04585 0.28 +1.0 855,543 + MMJJ_RS04585 0.28 +0.7 855,605 - MMJJ_RS04585 0.33 -1.0 855,610 - MMJJ_RS04585 0.33 +1.9 855,711 + MMJJ_RS04585 0.41 -0.0 855,794 - MMJJ_RS04585 0.47 -0.5 855,893 - MMJJ_RS04585 0.55 -0.6 855,943 + MMJJ_RS04585 0.59 +0.5 856,025 - MMJJ_RS04585 0.65 +0.8 856,025 - MMJJ_RS04585 0.65 -0.5
Or see this region's nucleotide sequence