Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03740 and MMJJ_RS03745 are separated by 46 nucleotides MMJJ_RS03745 and MMJJ_RS03750 are separated by 65 nucleotides MMJJ_RS03750 and MMJJ_RS03755 are separated by 20 nucleotides MMJJ_RS03755 and MMJJ_RS03760 are separated by 198 nucleotides MMJJ_RS03760 and MMJJ_RS03765 overlap by 1 nucleotides
MMJJ_RS03740: MMJJ_RS03740 - thiamine pyrophosphate-dependent enzyme, at 698,295 to 699,155
_RS03740
MMJJ_RS03745: MMJJ_RS03745 - N-glycosylase/DNA lyase, at 699,202 to 699,960
_RS03745
MMJJ_RS03750: MMJJ_RS03750 - rubredoxin, at 700,026 to 700,163
_RS03750
MMJJ_RS03755: MMJJ_RS03755 - hypothetical protein, at 700,184 to 700,543
_RS03755
MMJJ_RS03760: MMJJ_RS03760 - hydrogenase maturation nickel metallochaperone HypA, at 700,742 to 701,122
_RS03760
MMJJ_RS03765: MMJJ_RS03765 - hypothetical protein, at 701,122 to 701,583
_RS03765
Position (kb) 700
701 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 699.030 kb on - strand, within MMJJ_RS03740 at 699.072 kb on - strand at 699.073 kb on + strand at 699.073 kb on + strand at 699.157 kb on + strand at 699.167 kb on - strand at 699.167 kb on - strand at 699.167 kb on - strand at 699.168 kb on + strand at 699.168 kb on + strand at 699.168 kb on + strand at 699.171 kb on - strand at 699.179 kb on - strand at 699.240 kb on + strand at 699.267 kb on - strand at 699.271 kb on - strand at 699.271 kb on - strand at 699.271 kb on - strand at 699.271 kb on - strand at 699.271 kb on - strand at 699.272 kb on + strand at 699.272 kb on + strand at 699.272 kb on + strand at 699.272 kb on + strand at 699.378 kb on + strand, within MMJJ_RS03745 at 699.378 kb on + strand, within MMJJ_RS03745 at 699.378 kb on + strand, within MMJJ_RS03745 at 699.378 kb on + strand, within MMJJ_RS03745 at 699.378 kb on + strand, within MMJJ_RS03745 at 699.383 kb on + strand, within MMJJ_RS03745 at 699.389 kb on - strand, within MMJJ_RS03745 at 699.389 kb on - strand, within MMJJ_RS03745 at 699.389 kb on - strand, within MMJJ_RS03745 at 699.390 kb on + strand, within MMJJ_RS03745 at 699.390 kb on + strand, within MMJJ_RS03745 at 699.390 kb on + strand, within MMJJ_RS03745 at 699.390 kb on + strand, within MMJJ_RS03745 at 699.390 kb on + strand, within MMJJ_RS03745 at 699.393 kb on - strand, within MMJJ_RS03745 at 699.394 kb on + strand, within MMJJ_RS03745 at 699.453 kb on - strand, within MMJJ_RS03745 at 699.476 kb on + strand, within MMJJ_RS03745 at 699.519 kb on + strand, within MMJJ_RS03745 at 699.524 kb on - strand, within MMJJ_RS03745 at 699.525 kb on + strand, within MMJJ_RS03745 at 699.525 kb on + strand, within MMJJ_RS03745 at 699.691 kb on - strand, within MMJJ_RS03745 at 699.773 kb on + strand, within MMJJ_RS03745 at 699.773 kb on + strand, within MMJJ_RS03745 at 699.849 kb on + strand, within MMJJ_RS03745 at 699.854 kb on - strand, within MMJJ_RS03745 at 699.855 kb on + strand, within MMJJ_RS03745 at 699.866 kb on - strand, within MMJJ_RS03745 at 699.935 kb on + strand at 699.938 kb on - strand at 699.939 kb on + strand at 699.939 kb on + strand at 699.942 kb on - strand at 699.956 kb on - strand at 699.957 kb on + strand at 699.964 kb on + strand at 699.977 kb on - strand at 699.979 kb on - strand at 699.980 kb on + strand at 700.004 kb on - strand at 700.019 kb on + strand at 700.025 kb on - strand at 700.026 kb on + strand at 700.049 kb on + strand, within MMJJ_RS03750 at 700.057 kb on + strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand at 700.061 kb on + strand, within MMJJ_RS03750 at 700.062 kb on - strand, within MMJJ_RS03750 at 700.062 kb on - strand, within MMJJ_RS03750 at 700.062 kb on - strand, within MMJJ_RS03750 at 700.063 kb on + strand, within MMJJ_RS03750 at 700.162 kb on + strand at 700.192 kb on + strand at 700.192 kb on - strand at 700.209 kb on - strand at 700.209 kb on - strand at 700.210 kb on + strand at 700.210 kb on + strand at 700.210 kb on + strand at 700.210 kb on + strand at 700.210 kb on + strand at 700.214 kb on - strand at 700.215 kb on + strand at 700.218 kb on - strand at 700.219 kb on + strand at 700.219 kb on + strand at 700.256 kb on - strand, within MMJJ_RS03755 at 700.281 kb on - strand, within MMJJ_RS03755 at 700.281 kb on - strand, within MMJJ_RS03755 at 700.282 kb on + strand, within MMJJ_RS03755 at 700.282 kb on + strand, within MMJJ_RS03755 at 700.446 kb on + strand, within MMJJ_RS03755 at 700.446 kb on + strand, within MMJJ_RS03755 at 700.499 kb on - strand, within MMJJ_RS03755 at 700.523 kb on - strand at 700.589 kb on + strand at 700.596 kb on - strand at 700.598 kb on - strand at 700.598 kb on - strand at 700.598 kb on - strand at 700.599 kb on + strand at 700.600 kb on - strand at 700.601 kb on + strand at 700.601 kb on + strand at 700.601 kb on + strand at 700.604 kb on + strand at 700.635 kb on - strand at 700.636 kb on + strand at 700.681 kb on - strand at 700.685 kb on - strand at 700.685 kb on - strand at 700.685 kb on - strand at 700.695 kb on - strand at 700.695 kb on - strand at 700.695 kb on - strand at 700.695 kb on - strand at 700.696 kb on + strand at 700.696 kb on + strand at 700.696 kb on + strand at 700.696 kb on + strand at 700.696 kb on + strand at 700.697 kb on - strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.712 kb on + strand at 700.727 kb on + strand at 700.727 kb on + strand at 700.730 kb on - strand at 700.731 kb on + strand at 700.778 kb on - strand at 700.808 kb on + strand, within MMJJ_RS03760 at 700.835 kb on - strand at 700.835 kb on - strand, within MMJJ_RS03760 at 700.863 kb on + strand, within MMJJ_RS03760 at 700.942 kb on - strand, within MMJJ_RS03760 at 700.946 kb on + strand, within MMJJ_RS03760 at 700.991 kb on - strand, within MMJJ_RS03760 at 701.021 kb on - strand, within MMJJ_RS03760 at 701.022 kb on + strand, within MMJJ_RS03760 at 701.023 kb on - strand, within MMJJ_RS03760 at 701.031 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 699,030 - MMJJ_RS03740 0.85 -3.4 699,072 - +2.6 699,073 + -1.6 699,073 + +0.2 699,157 + +1.0 699,167 - +3.0 699,167 - +3.0 699,167 - +0.8 699,168 + -3.1 699,168 + +1.9 699,168 + +0.8 699,171 - +0.8 699,179 - -0.1 699,240 + -1.9 699,267 - -2.2 699,271 - -4.2 699,271 - -1.9 699,271 - +0.1 699,271 - -1.1 699,271 - -0.8 699,272 + +1.2 699,272 + +0.2 699,272 + -2.0 699,272 + -0.9 699,378 + MMJJ_RS03745 0.23 +1.9 699,378 + MMJJ_RS03745 0.23 +0.4 699,378 + MMJJ_RS03745 0.23 -4.3 699,378 + MMJJ_RS03745 0.23 -3.7 699,378 + MMJJ_RS03745 0.23 -2.1 699,383 + MMJJ_RS03745 0.24 +1.2 699,389 - MMJJ_RS03745 0.25 +1.3 699,389 - MMJJ_RS03745 0.25 +0.0 699,389 - MMJJ_RS03745 0.25 -1.9 699,390 + MMJJ_RS03745 0.25 -0.5 699,390 + MMJJ_RS03745 0.25 +1.1 699,390 + MMJJ_RS03745 0.25 +0.9 699,390 + MMJJ_RS03745 0.25 -1.0 699,390 + MMJJ_RS03745 0.25 -0.6 699,393 - MMJJ_RS03745 0.25 +1.6 699,394 + MMJJ_RS03745 0.25 -2.7 699,453 - MMJJ_RS03745 0.33 +0.4 699,476 + MMJJ_RS03745 0.36 +1.3 699,519 + MMJJ_RS03745 0.42 -1.3 699,524 - MMJJ_RS03745 0.42 -2.4 699,525 + MMJJ_RS03745 0.43 +0.9 699,525 + MMJJ_RS03745 0.43 +0.8 699,691 - MMJJ_RS03745 0.64 +0.8 699,773 + MMJJ_RS03745 0.75 -2.3 699,773 + MMJJ_RS03745 0.75 -1.4 699,849 + MMJJ_RS03745 0.85 -0.7 699,854 - MMJJ_RS03745 0.86 -0.3 699,855 + MMJJ_RS03745 0.86 +0.5 699,866 - MMJJ_RS03745 0.87 +0.7 699,935 + -0.2 699,938 - +0.4 699,939 + -0.1 699,939 + +1.0 699,942 - +0.7 699,956 - -4.8 699,957 + +1.6 699,964 + -1.3 699,977 - +0.8 699,979 - -3.0 699,980 + -0.3 700,004 - +1.2 700,019 + -0.1 700,025 - -1.3 700,026 + +1.7 700,049 + MMJJ_RS03750 0.17 +3.2 700,057 + MMJJ_RS03750 0.22 -0.8 700,060 - MMJJ_RS03750 0.25 +0.6 700,060 - MMJJ_RS03750 0.25 -1.3 700,060 - +1.5 700,060 - MMJJ_RS03750 0.25 -0.2 700,060 - MMJJ_RS03750 0.25 +0.4 700,060 - MMJJ_RS03750 0.25 +1.0 700,060 - MMJJ_RS03750 0.25 +0.8 700,060 - MMJJ_RS03750 0.25 -3.9 700,060 - MMJJ_RS03750 0.25 +0.3 700,060 - MMJJ_RS03750 0.25 +1.3 700,060 - MMJJ_RS03750 0.25 -1.4 700,060 - MMJJ_RS03750 0.25 -4.4 700,060 - MMJJ_RS03750 0.25 +0.9 700,061 + MMJJ_RS03750 0.25 -3.3 700,061 + MMJJ_RS03750 0.25 +0.6 700,061 + MMJJ_RS03750 0.25 +0.6 700,061 + MMJJ_RS03750 0.25 +3.0 700,061 + MMJJ_RS03750 0.25 -0.6 700,061 + MMJJ_RS03750 0.25 +1.2 700,061 + MMJJ_RS03750 0.25 +1.1 700,061 + MMJJ_RS03750 0.25 -3.3 700,061 + MMJJ_RS03750 0.25 +0.1 700,061 + MMJJ_RS03750 0.25 -1.3 700,061 + MMJJ_RS03750 0.25 +0.5 700,061 + MMJJ_RS03750 0.25 +0.6 700,061 + MMJJ_RS03750 0.25 +2.9 700,061 + MMJJ_RS03750 0.25 +0.9 700,061 + +0.5 700,061 + MMJJ_RS03750 0.25 +1.0 700,062 - MMJJ_RS03750 0.26 +1.2 700,062 - MMJJ_RS03750 0.26 +0.7 700,062 - MMJJ_RS03750 0.26 -1.1 700,063 + MMJJ_RS03750 0.27 -0.1 700,162 + +0.9 700,192 + -4.4 700,192 - -1.3 700,209 - -0.1 700,209 - -2.6 700,210 + -1.8 700,210 + -1.6 700,210 + -2.9 700,210 + -0.3 700,210 + +2.0 700,214 - -0.0 700,215 + +0.7 700,218 - +1.5 700,219 + -0.3 700,219 + -0.2 700,256 - MMJJ_RS03755 0.20 +1.0 700,281 - MMJJ_RS03755 0.27 -0.4 700,281 - MMJJ_RS03755 0.27 -0.9 700,282 + MMJJ_RS03755 0.27 -0.5 700,282 + MMJJ_RS03755 0.27 -1.4 700,446 + MMJJ_RS03755 0.73 +1.2 700,446 + MMJJ_RS03755 0.73 -0.3 700,499 - MMJJ_RS03755 0.88 -1.3 700,523 - -0.3 700,589 + +1.0 700,596 - +0.2 700,598 - -0.5 700,598 - +2.1 700,598 - +1.0 700,599 + -2.0 700,600 - +0.1 700,601 + +0.2 700,601 + -0.0 700,601 + +0.9 700,604 + -2.6 700,635 - +1.9 700,636 + -1.0 700,681 - +0.3 700,685 - +0.1 700,685 - -0.3 700,685 - -1.4 700,695 - -2.5 700,695 - +0.2 700,695 - -1.1 700,695 - -1.6 700,696 + +1.0 700,696 + +1.4 700,696 + -0.4 700,696 + -1.8 700,696 + +1.4 700,697 - -0.7 700,698 + -0.9 700,698 + -1.9 700,698 + +0.8 700,698 + +1.4 700,698 + +1.5 700,712 + -3.2 700,727 + -0.8 700,727 + +1.7 700,730 - +0.4 700,731 + -2.5 700,778 - -2.0 700,808 + MMJJ_RS03760 0.17 +0.6 700,835 - -1.3 700,835 - MMJJ_RS03760 0.24 -2.8 700,863 + MMJJ_RS03760 0.32 +0.6 700,942 - MMJJ_RS03760 0.52 -2.4 700,946 + MMJJ_RS03760 0.54 -1.8 700,991 - MMJJ_RS03760 0.65 -2.4 701,021 - MMJJ_RS03760 0.73 -0.4 701,022 + MMJJ_RS03760 0.73 -1.0 701,023 - MMJJ_RS03760 0.74 -2.3 701,031 + -0.0
Or see this region's nucleotide sequence