Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03725 and MMJJ_RS03730 are separated by 156 nucleotides MMJJ_RS03730 and MMJJ_RS03735 are separated by 72 nucleotides MMJJ_RS03735 and MMJJ_RS03740 are separated by 5 nucleotides MMJJ_RS03740 and MMJJ_RS03745 are separated by 46 nucleotides
MMJJ_RS03725: MMJJ_RS03725 - sulfurtransferase TusA family protein, at 695,446 to 695,658
_RS03725
MMJJ_RS03730: MMJJ_RS03730 - SWIM zinc finger family protein, at 695,815 to 696,504
_RS03730
MMJJ_RS03735: MMJJ_RS03735 - 2-oxoacid:acceptor oxidoreductase subunit alpha, at 696,577 to 698,289
_RS03735
MMJJ_RS03740: MMJJ_RS03740 - thiamine pyrophosphate-dependent enzyme, at 698,295 to 699,155
_RS03740
MMJJ_RS03745: MMJJ_RS03745 - N-glycosylase/DNA lyase, at 699,202 to 699,960
_RS03745
Position (kb) 696
697
698
699 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 695.632 kb on - strand, within MMJJ_RS03725 at 695.681 kb on - strand at 695.687 kb on - strand at 695.732 kb on + strand at 695.747 kb on + strand at 695.755 kb on + strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.764 kb on + strand at 695.764 kb on + strand at 695.764 kb on + strand at 695.764 kb on + strand at 695.768 kb on + strand at 695.771 kb on - strand at 695.784 kb on - strand at 695.837 kb on - strand at 695.838 kb on + strand at 695.838 kb on + strand at 695.844 kb on + strand at 695.863 kb on - strand at 695.864 kb on + strand at 695.864 kb on + strand at 695.883 kb on + strand at 695.917 kb on + strand, within MMJJ_RS03730 at 695.956 kb on - strand, within MMJJ_RS03730 at 695.968 kb on - strand, within MMJJ_RS03730 at 695.999 kb on - strand, within MMJJ_RS03730 at 696.000 kb on + strand, within MMJJ_RS03730 at 696.000 kb on + strand, within MMJJ_RS03730 at 696.000 kb on + strand, within MMJJ_RS03730 at 696.085 kb on + strand, within MMJJ_RS03730 at 696.229 kb on - strand, within MMJJ_RS03730 at 696.321 kb on + strand, within MMJJ_RS03730 at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.488 kb on - strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.489 kb on + strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.490 kb on - strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.491 kb on + strand at 696.528 kb on - strand at 696.528 kb on - strand at 696.528 kb on - strand at 696.530 kb on - strand at 696.532 kb on - strand at 696.532 kb on - strand at 696.532 kb on - strand at 696.533 kb on + strand at 696.533 kb on + strand at 696.533 kb on + strand at 696.533 kb on + strand at 696.534 kb on - strand at 696.534 kb on - strand at 696.535 kb on + strand at 696.550 kb on - strand at 696.550 kb on - strand at 696.550 kb on - strand at 696.550 kb on - strand at 696.551 kb on + strand at 696.551 kb on + strand at 696.551 kb on + strand at 696.552 kb on - strand at 696.553 kb on + strand at 696.553 kb on + strand at 696.558 kb on - strand at 696.558 kb on - strand at 696.558 kb on - strand at 696.576 kb on - strand at 696.577 kb on + strand at 696.632 kb on - strand at 696.663 kb on + strand at 696.679 kb on - strand at 696.679 kb on - strand at 696.680 kb on + strand at 696.680 kb on + strand at 696.686 kb on - strand at 696.686 kb on - strand at 696.686 kb on - strand at 696.687 kb on + strand at 696.694 kb on + strand at 696.703 kb on - strand at 696.704 kb on + strand at 696.704 kb on + strand at 696.704 kb on + strand at 696.746 kb on - strand at 696.746 kb on - strand at 696.849 kb on - strand, within MMJJ_RS03735 at 696.876 kb on - strand, within MMJJ_RS03735 at 696.932 kb on - strand, within MMJJ_RS03735 at 696.933 kb on + strand, within MMJJ_RS03735 at 696.933 kb on + strand, within MMJJ_RS03735 at 696.987 kb on + strand, within MMJJ_RS03735 at 697.080 kb on + strand, within MMJJ_RS03735 at 697.100 kb on + strand at 697.118 kb on - strand, within MMJJ_RS03735 at 697.137 kb on + strand, within MMJJ_RS03735 at 697.137 kb on + strand, within MMJJ_RS03735 at 697.184 kb on - strand, within MMJJ_RS03735 at 697.191 kb on - strand, within MMJJ_RS03735 at 697.230 kb on - strand, within MMJJ_RS03735 at 697.230 kb on - strand, within MMJJ_RS03735 at 697.231 kb on + strand, within MMJJ_RS03735 at 697.311 kb on - strand, within MMJJ_RS03735 at 697.311 kb on - strand, within MMJJ_RS03735 at 697.316 kb on - strand, within MMJJ_RS03735 at 697.317 kb on + strand, within MMJJ_RS03735 at 697.350 kb on - strand, within MMJJ_RS03735 at 697.350 kb on - strand, within MMJJ_RS03735 at 697.392 kb on - strand, within MMJJ_RS03735 at 697.392 kb on - strand, within MMJJ_RS03735 at 697.396 kb on + strand, within MMJJ_RS03735 at 697.470 kb on + strand, within MMJJ_RS03735 at 697.471 kb on - strand, within MMJJ_RS03735 at 697.471 kb on - strand, within MMJJ_RS03735 at 697.532 kb on - strand, within MMJJ_RS03735 at 697.570 kb on + strand, within MMJJ_RS03735 at 697.575 kb on - strand, within MMJJ_RS03735 at 697.578 kb on + strand, within MMJJ_RS03735 at 697.578 kb on + strand, within MMJJ_RS03735 at 697.614 kb on + strand, within MMJJ_RS03735 at 697.679 kb on + strand, within MMJJ_RS03735 at 697.693 kb on - strand, within MMJJ_RS03735 at 697.761 kb on + strand, within MMJJ_RS03735 at 697.872 kb on - strand, within MMJJ_RS03735 at 697.876 kb on + strand, within MMJJ_RS03735 at 697.929 kb on + strand, within MMJJ_RS03735 at 697.935 kb on + strand, within MMJJ_RS03735 at 697.981 kb on - strand, within MMJJ_RS03735 at 698.017 kb on - strand, within MMJJ_RS03735 at 698.025 kb on + strand, within MMJJ_RS03735 at 698.058 kb on - strand, within MMJJ_RS03735 at 698.059 kb on + strand, within MMJJ_RS03735 at 698.142 kb on - strand at 698.142 kb on - strand at 698.151 kb on + strand at 698.212 kb on + strand at 698.214 kb on - strand at 698.220 kb on + strand at 698.329 kb on - strand at 698.329 kb on - strand at 698.330 kb on + strand at 698.333 kb on - strand at 698.334 kb on + strand at 698.334 kb on + strand at 698.334 kb on + strand at 698.357 kb on - strand at 698.358 kb on + strand at 698.369 kb on + strand at 698.466 kb on - strand, within MMJJ_RS03740 at 698.466 kb on - strand, within MMJJ_RS03740 at 698.470 kb on - strand, within MMJJ_RS03740 at 698.470 kb on - strand, within MMJJ_RS03740 at 698.477 kb on + strand, within MMJJ_RS03740 at 698.550 kb on + strand, within MMJJ_RS03740 at 698.583 kb on - strand, within MMJJ_RS03740 at 698.583 kb on - strand, within MMJJ_RS03740 at 698.583 kb on - strand, within MMJJ_RS03740 at 698.583 kb on - strand, within MMJJ_RS03740 at 698.583 kb on - strand, within MMJJ_RS03740 at 698.583 kb on - strand, within MMJJ_RS03740 at 698.583 kb on - strand, within MMJJ_RS03740 at 698.583 kb on - strand, within MMJJ_RS03740 at 698.584 kb on + strand, within MMJJ_RS03740 at 698.584 kb on + strand, within MMJJ_RS03740 at 698.584 kb on + strand, within MMJJ_RS03740 at 698.584 kb on + strand, within MMJJ_RS03740 at 698.644 kb on - strand, within MMJJ_RS03740 at 698.645 kb on + strand, within MMJJ_RS03740 at 698.645 kb on + strand, within MMJJ_RS03740 at 698.655 kb on + strand, within MMJJ_RS03740 at 698.662 kb on - strand, within MMJJ_RS03740 at 698.663 kb on + strand, within MMJJ_RS03740 at 698.663 kb on + strand, within MMJJ_RS03740 at 698.663 kb on + strand, within MMJJ_RS03740 at 698.663 kb on + strand, within MMJJ_RS03740 at 698.665 kb on + strand, within MMJJ_RS03740 at 698.780 kb on + strand, within MMJJ_RS03740 at 698.839 kb on - strand, within MMJJ_RS03740 at 698.844 kb on + strand, within MMJJ_RS03740 at 698.872 kb on - strand, within MMJJ_RS03740 at 698.875 kb on - strand, within MMJJ_RS03740 at 698.879 kb on - strand, within MMJJ_RS03740 at 698.879 kb on - strand, within MMJJ_RS03740 at 698.879 kb on - strand, within MMJJ_RS03740 at 698.912 kb on + strand, within MMJJ_RS03740 at 698.928 kb on - strand, within MMJJ_RS03740 at 698.965 kb on - strand, within MMJJ_RS03740 at 698.965 kb on - strand, within MMJJ_RS03740 at 698.966 kb on + strand, within MMJJ_RS03740 at 698.966 kb on + strand, within MMJJ_RS03740 at 698.969 kb on - strand, within MMJJ_RS03740 at 699.030 kb on - strand, within MMJJ_RS03740 at 699.072 kb on - strand at 699.073 kb on + strand at 699.073 kb on + strand at 699.157 kb on + strand at 699.167 kb on - strand at 699.167 kb on - strand at 699.167 kb on - strand at 699.168 kb on + strand at 699.168 kb on + strand at 699.168 kb on + strand at 699.171 kb on - strand at 699.179 kb on - strand at 699.240 kb on + strand at 699.267 kb on - strand at 699.271 kb on - strand at 699.271 kb on - strand at 699.271 kb on - strand at 699.271 kb on - strand at 699.271 kb on - strand at 699.272 kb on + strand at 699.272 kb on + strand at 699.272 kb on + strand at 699.272 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 695,632 - MMJJ_RS03725 0.87 -1.5 695,681 - +0.4 695,687 - -0.8 695,732 + +1.1 695,747 + +2.2 695,755 + -3.3 695,763 - -0.1 695,763 - +0.1 695,763 - -4.4 695,763 - +2.3 695,763 - -3.8 695,764 + -0.4 695,764 + -0.5 695,764 + +0.1 695,764 + -0.0 695,768 + -1.2 695,771 - -4.0 695,784 - +0.5 695,837 - -1.1 695,838 + -0.3 695,838 + -3.6 695,844 + +0.2 695,863 - -3.5 695,864 + +0.2 695,864 + -1.5 695,883 + +0.5 695,917 + MMJJ_RS03730 0.15 -0.1 695,956 - MMJJ_RS03730 0.20 -0.3 695,968 - MMJJ_RS03730 0.22 +0.1 695,999 - MMJJ_RS03730 0.27 +1.4 696,000 + MMJJ_RS03730 0.27 +1.0 696,000 + MMJJ_RS03730 0.27 +1.3 696,000 + MMJJ_RS03730 0.27 -3.0 696,085 + MMJJ_RS03730 0.39 +4.3 696,229 - MMJJ_RS03730 0.60 +0.1 696,321 + MMJJ_RS03730 0.73 -3.9 696,488 - -0.4 696,488 - +2.1 696,488 - -3.1 696,488 - -2.6 696,488 - -0.5 696,488 - -1.9 696,488 - -0.2 696,488 - -0.7 696,488 - -1.0 696,488 - +1.3 696,489 + +3.0 696,489 + -1.7 696,489 + -4.2 696,489 + +0.7 696,489 + -3.2 696,489 + -1.1 696,489 + +0.2 696,490 - +1.3 696,490 - +2.7 696,490 - -1.8 696,490 - +0.3 696,490 - -2.4 696,490 - -1.4 696,490 - +0.7 696,490 - -1.0 696,490 - -2.8 696,490 - +1.1 696,490 - -1.3 696,490 - -1.8 696,490 - +0.1 696,490 - +0.7 696,491 + +1.7 696,491 + -0.6 696,491 + -1.1 696,491 + +1.0 696,491 + +1.0 696,491 + +1.3 696,491 + +0.8 696,491 + +1.2 696,491 + +0.5 696,491 + -2.9 696,491 + -0.2 696,528 - -0.5 696,528 - -1.3 696,528 - -0.2 696,530 - -2.0 696,532 - +1.2 696,532 - -2.2 696,532 - -0.5 696,533 + -1.2 696,533 + -1.3 696,533 + +0.0 696,533 + +3.2 696,534 - -0.1 696,534 - +0.2 696,535 + +1.6 696,550 - -2.3 696,550 - +1.6 696,550 - -1.1 696,550 - +0.0 696,551 + +2.3 696,551 + +1.5 696,551 + +0.2 696,552 - -0.4 696,553 + +1.2 696,553 + +0.2 696,558 - -0.4 696,558 - -1.5 696,558 - -0.5 696,576 - -2.3 696,577 + +1.3 696,632 - +0.8 696,663 + -0.0 696,679 - -0.8 696,679 - -0.2 696,680 + -4.4 696,680 + -1.7 696,686 - -1.1 696,686 - +0.1 696,686 - +0.8 696,687 + +2.1 696,694 + +3.5 696,703 - -1.1 696,704 + +1.1 696,704 + +2.7 696,704 + +1.6 696,746 - -4.9 696,746 - -2.6 696,849 - MMJJ_RS03735 0.16 +1.5 696,876 - MMJJ_RS03735 0.17 +0.6 696,932 - MMJJ_RS03735 0.21 -1.0 696,933 + MMJJ_RS03735 0.21 -0.8 696,933 + MMJJ_RS03735 0.21 +1.5 696,987 + MMJJ_RS03735 0.24 +1.2 697,080 + MMJJ_RS03735 0.29 +0.3 697,100 + -0.1 697,118 - MMJJ_RS03735 0.32 +1.1 697,137 + MMJJ_RS03735 0.33 +0.7 697,137 + MMJJ_RS03735 0.33 -0.9 697,184 - MMJJ_RS03735 0.35 -0.5 697,191 - MMJJ_RS03735 0.36 +0.8 697,230 - MMJJ_RS03735 0.38 +1.8 697,230 - MMJJ_RS03735 0.38 -2.9 697,231 + MMJJ_RS03735 0.38 -0.8 697,311 - MMJJ_RS03735 0.43 -1.1 697,311 - MMJJ_RS03735 0.43 +0.7 697,316 - MMJJ_RS03735 0.43 -2.2 697,317 + MMJJ_RS03735 0.43 +1.9 697,350 - MMJJ_RS03735 0.45 +0.7 697,350 - MMJJ_RS03735 0.45 +1.8 697,392 - MMJJ_RS03735 0.48 +0.9 697,392 - MMJJ_RS03735 0.48 +0.3 697,396 + MMJJ_RS03735 0.48 +1.6 697,470 + MMJJ_RS03735 0.52 -0.8 697,471 - MMJJ_RS03735 0.52 +0.9 697,471 - MMJJ_RS03735 0.52 +1.4 697,532 - MMJJ_RS03735 0.56 +1.4 697,570 + MMJJ_RS03735 0.58 +0.8 697,575 - MMJJ_RS03735 0.58 -4.1 697,578 + MMJJ_RS03735 0.58 +1.7 697,578 + MMJJ_RS03735 0.58 -0.1 697,614 + MMJJ_RS03735 0.61 +1.6 697,679 + MMJJ_RS03735 0.64 +1.4 697,693 - MMJJ_RS03735 0.65 +1.2 697,761 + MMJJ_RS03735 0.69 +1.6 697,872 - MMJJ_RS03735 0.76 +0.7 697,876 + MMJJ_RS03735 0.76 -0.4 697,929 + MMJJ_RS03735 0.79 -0.1 697,935 + MMJJ_RS03735 0.79 -0.3 697,981 - MMJJ_RS03735 0.82 +1.4 698,017 - MMJJ_RS03735 0.84 +0.8 698,025 + MMJJ_RS03735 0.85 -0.4 698,058 - MMJJ_RS03735 0.86 +1.0 698,059 + MMJJ_RS03735 0.87 +2.1 698,142 - +1.4 698,142 - +0.5 698,151 + -1.2 698,212 + -1.6 698,214 - -0.4 698,220 + +1.3 698,329 - -2.7 698,329 - +4.3 698,330 + +0.9 698,333 - -1.6 698,334 + +1.6 698,334 + -0.4 698,334 + +1.7 698,357 - +1.2 698,358 + +0.7 698,369 + +0.2 698,466 - MMJJ_RS03740 0.20 -2.9 698,466 - MMJJ_RS03740 0.20 +0.2 698,470 - MMJJ_RS03740 0.20 -0.2 698,470 - MMJJ_RS03740 0.20 +1.2 698,477 + MMJJ_RS03740 0.21 +0.4 698,550 + MMJJ_RS03740 0.30 +0.2 698,583 - MMJJ_RS03740 0.33 +0.0 698,583 - MMJJ_RS03740 0.33 -0.7 698,583 - MMJJ_RS03740 0.33 +0.5 698,583 - MMJJ_RS03740 0.33 +2.1 698,583 - MMJJ_RS03740 0.33 -2.0 698,583 - MMJJ_RS03740 0.33 -1.5 698,583 - MMJJ_RS03740 0.33 -0.3 698,583 - MMJJ_RS03740 0.33 -1.1 698,584 + MMJJ_RS03740 0.34 +1.3 698,584 + MMJJ_RS03740 0.34 -3.3 698,584 + MMJJ_RS03740 0.34 -1.8 698,584 + MMJJ_RS03740 0.34 +0.1 698,644 - MMJJ_RS03740 0.41 -1.1 698,645 + MMJJ_RS03740 0.41 +0.4 698,645 + MMJJ_RS03740 0.41 +0.5 698,655 + MMJJ_RS03740 0.42 +0.0 698,662 - MMJJ_RS03740 0.43 -1.8 698,663 + MMJJ_RS03740 0.43 +2.4 698,663 + MMJJ_RS03740 0.43 +0.9 698,663 + MMJJ_RS03740 0.43 -2.9 698,663 + MMJJ_RS03740 0.43 +0.1 698,665 + MMJJ_RS03740 0.43 +0.7 698,780 + MMJJ_RS03740 0.56 +0.1 698,839 - MMJJ_RS03740 0.63 -4.7 698,844 + MMJJ_RS03740 0.64 -2.3 698,872 - MMJJ_RS03740 0.67 -2.4 698,875 - MMJJ_RS03740 0.67 -0.1 698,879 - MMJJ_RS03740 0.68 +0.1 698,879 - MMJJ_RS03740 0.68 +0.3 698,879 - MMJJ_RS03740 0.68 +1.7 698,912 + MMJJ_RS03740 0.72 -1.9 698,928 - MMJJ_RS03740 0.74 -2.4 698,965 - MMJJ_RS03740 0.78 +2.2 698,965 - MMJJ_RS03740 0.78 -1.8 698,966 + MMJJ_RS03740 0.78 -0.4 698,966 + MMJJ_RS03740 0.78 -0.0 698,969 - MMJJ_RS03740 0.78 +1.3 699,030 - MMJJ_RS03740 0.85 -3.4 699,072 - +2.6 699,073 + -1.6 699,073 + +0.2 699,157 + +1.0 699,167 - +3.0 699,167 - +3.0 699,167 - +0.8 699,168 + -3.1 699,168 + +1.9 699,168 + +0.8 699,171 - +0.8 699,179 - -0.1 699,240 + -1.9 699,267 - -2.2 699,271 - -4.2 699,271 - -1.9 699,271 - +0.1 699,271 - -1.1 699,271 - -0.8 699,272 + +1.2 699,272 + +0.2 699,272 + -2.0 699,272 + -0.9
Or see this region's nucleotide sequence