Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03125 and MMJJ_RS03130 are separated by 21 nucleotides MMJJ_RS03130 and MMJJ_RS03135 are separated by 158 nucleotides MMJJ_RS03135 and MMJJ_RS03140 are separated by 14 nucleotides
MMJJ_RS03125: MMJJ_RS03125 - GMC oxidoreductase, at 590,224 to 591,384
_RS03125
MMJJ_RS03130: MMJJ_RS03130 - DUF1611 domain-containing protein, at 591,406 to 592,326
_RS03130
MMJJ_RS03135: MMJJ_RS03135 - CBS domain-containing protein, at 592,485 to 592,898
_RS03135
MMJJ_RS03140: MMJJ_RS03140 - tRNA-dihydrouridine synthase, at 592,913 to 593,683
_RS03140
Position (kb) 591
592
593 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 590.468 kb on - strand, within MMJJ_RS03125 at 590.469 kb on + strand at 590.469 kb on + strand, within MMJJ_RS03125 at 590.470 kb on - strand, within MMJJ_RS03125 at 590.706 kb on + strand at 590.736 kb on + strand, within MMJJ_RS03125 at 590.763 kb on - strand, within MMJJ_RS03125 at 590.770 kb on - strand, within MMJJ_RS03125 at 590.834 kb on - strand, within MMJJ_RS03125 at 590.835 kb on + strand, within MMJJ_RS03125 at 590.838 kb on + strand, within MMJJ_RS03125 at 590.888 kb on - strand, within MMJJ_RS03125 at 591.283 kb on - strand at 591.342 kb on - strand at 591.439 kb on - strand at 591.439 kb on - strand at 591.440 kb on + strand at 591.482 kb on + strand at 591.580 kb on - strand, within MMJJ_RS03130 at 591.580 kb on - strand, within MMJJ_RS03130 at 591.760 kb on - strand, within MMJJ_RS03130 at 591.903 kb on - strand, within MMJJ_RS03130 at 591.985 kb on - strand, within MMJJ_RS03130 at 591.994 kb on + strand, within MMJJ_RS03130 at 592.022 kb on - strand, within MMJJ_RS03130 at 592.022 kb on - strand, within MMJJ_RS03130 at 592.022 kb on - strand, within MMJJ_RS03130 at 592.023 kb on + strand, within MMJJ_RS03130 at 592.030 kb on - strand, within MMJJ_RS03130 at 592.031 kb on + strand, within MMJJ_RS03130 at 592.064 kb on - strand, within MMJJ_RS03130 at 592.065 kb on + strand, within MMJJ_RS03130 at 592.067 kb on - strand, within MMJJ_RS03130 at 592.067 kb on - strand, within MMJJ_RS03130 at 592.072 kb on - strand, within MMJJ_RS03130 at 592.079 kb on - strand, within MMJJ_RS03130 at 592.079 kb on - strand, within MMJJ_RS03130 at 592.142 kb on - strand, within MMJJ_RS03130 at 592.142 kb on - strand, within MMJJ_RS03130 at 592.142 kb on - strand, within MMJJ_RS03130 at 592.143 kb on + strand, within MMJJ_RS03130 at 592.146 kb on - strand, within MMJJ_RS03130 at 592.147 kb on + strand, within MMJJ_RS03130 at 592.150 kb on + strand, within MMJJ_RS03130 at 592.150 kb on + strand, within MMJJ_RS03130 at 592.162 kb on + strand, within MMJJ_RS03130 at 592.213 kb on - strand, within MMJJ_RS03130 at 592.214 kb on + strand, within MMJJ_RS03130 at 592.214 kb on + strand, within MMJJ_RS03130 at 592.230 kb on - strand, within MMJJ_RS03130 at 592.230 kb on - strand, within MMJJ_RS03130 at 592.231 kb on + strand, within MMJJ_RS03130 at 592.231 kb on + strand, within MMJJ_RS03130 at 592.241 kb on - strand at 592.255 kb on - strand at 592.255 kb on - strand at 592.255 kb on - strand at 592.256 kb on + strand at 592.256 kb on + strand at 592.326 kb on - strand at 592.327 kb on + strand at 592.329 kb on + strand at 592.365 kb on - strand at 592.425 kb on - strand at 592.425 kb on - strand at 592.425 kb on - strand at 592.425 kb on - strand at 592.439 kb on - strand at 592.439 kb on - strand at 592.448 kb on - strand at 592.448 kb on - strand at 592.449 kb on + strand at 592.482 kb on + strand at 592.504 kb on - strand at 592.517 kb on - strand at 592.597 kb on - strand, within MMJJ_RS03135 at 592.660 kb on - strand, within MMJJ_RS03135 at 592.660 kb on - strand, within MMJJ_RS03135 at 592.661 kb on + strand, within MMJJ_RS03135 at 592.665 kb on - strand, within MMJJ_RS03135 at 592.672 kb on - strand, within MMJJ_RS03135 at 592.673 kb on + strand, within MMJJ_RS03135 at 592.673 kb on + strand, within MMJJ_RS03135 at 592.688 kb on - strand, within MMJJ_RS03135 at 592.688 kb on - strand, within MMJJ_RS03135 at 592.693 kb on - strand, within MMJJ_RS03135 at 592.868 kb on - strand at 592.891 kb on - strand at 592.892 kb on + strand at 592.892 kb on + strand at 592.910 kb on - strand at 592.911 kb on + strand at 592.921 kb on - strand at 592.921 kb on - strand at 592.921 kb on - strand at 592.922 kb on + strand at 592.935 kb on + strand at 592.940 kb on + strand at 592.949 kb on - strand at 593.079 kb on + strand, within MMJJ_RS03140 at 593.105 kb on + strand, within MMJJ_RS03140 at 593.120 kb on - strand, within MMJJ_RS03140 at 593.120 kb on - strand, within MMJJ_RS03140 at 593.134 kb on + strand, within MMJJ_RS03140 at 593.135 kb on - strand, within MMJJ_RS03140 at 593.135 kb on - strand, within MMJJ_RS03140 at 593.136 kb on + strand, within MMJJ_RS03140 at 593.136 kb on + strand, within MMJJ_RS03140 at 593.136 kb on + strand, within MMJJ_RS03140 at 593.140 kb on - strand, within MMJJ_RS03140 at 593.253 kb on + strand, within MMJJ_RS03140 at 593.253 kb on + strand, within MMJJ_RS03140 at 593.307 kb on + strand, within MMJJ_RS03140
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 590,468 - MMJJ_RS03125 0.21 -2.3 590,469 + -0.8 590,469 + MMJJ_RS03125 0.21 -0.5 590,470 - MMJJ_RS03125 0.21 +0.5 590,706 + +0.5 590,736 + MMJJ_RS03125 0.44 -2.1 590,763 - MMJJ_RS03125 0.46 +0.5 590,770 - MMJJ_RS03125 0.47 -0.8 590,834 - MMJJ_RS03125 0.53 -3.1 590,835 + MMJJ_RS03125 0.53 -3.1 590,838 + MMJJ_RS03125 0.53 -2.5 590,888 - MMJJ_RS03125 0.57 -2.8 591,283 - -0.5 591,342 - -2.4 591,439 - +0.1 591,439 - -4.6 591,440 + +0.3 591,482 + -0.5 591,580 - MMJJ_RS03130 0.19 -0.8 591,580 - MMJJ_RS03130 0.19 -0.5 591,760 - MMJJ_RS03130 0.38 -3.7 591,903 - MMJJ_RS03130 0.54 -0.2 591,985 - MMJJ_RS03130 0.63 +0.5 591,994 + MMJJ_RS03130 0.64 +1.6 592,022 - MMJJ_RS03130 0.67 +2.1 592,022 - MMJJ_RS03130 0.67 -4.0 592,022 - MMJJ_RS03130 0.67 -0.1 592,023 + MMJJ_RS03130 0.67 +1.7 592,030 - MMJJ_RS03130 0.68 -0.5 592,031 + MMJJ_RS03130 0.68 +1.3 592,064 - MMJJ_RS03130 0.71 -0.8 592,065 + MMJJ_RS03130 0.72 +1.6 592,067 - MMJJ_RS03130 0.72 -0.4 592,067 - MMJJ_RS03130 0.72 -1.1 592,072 - MMJJ_RS03130 0.72 -1.5 592,079 - MMJJ_RS03130 0.73 -1.7 592,079 - MMJJ_RS03130 0.73 +0.7 592,142 - MMJJ_RS03130 0.80 -0.8 592,142 - MMJJ_RS03130 0.80 +3.5 592,142 - MMJJ_RS03130 0.80 -1.4 592,143 + MMJJ_RS03130 0.80 +1.8 592,146 - MMJJ_RS03130 0.80 +0.3 592,147 + MMJJ_RS03130 0.80 +1.8 592,150 + MMJJ_RS03130 0.81 -0.5 592,150 + MMJJ_RS03130 0.81 +0.8 592,162 + MMJJ_RS03130 0.82 -1.7 592,213 - MMJJ_RS03130 0.88 -3.4 592,214 + MMJJ_RS03130 0.88 +1.6 592,214 + MMJJ_RS03130 0.88 +2.8 592,230 - MMJJ_RS03130 0.89 -0.7 592,230 - MMJJ_RS03130 0.89 +2.9 592,231 + MMJJ_RS03130 0.90 +1.1 592,231 + MMJJ_RS03130 0.90 +2.1 592,241 - -2.5 592,255 - +1.0 592,255 - -1.9 592,255 - -0.9 592,256 + -2.8 592,256 + +1.1 592,326 - +0.8 592,327 + +0.4 592,329 + -4.8 592,365 - +0.8 592,425 - +0.2 592,425 - +0.6 592,425 - +1.2 592,425 - +1.4 592,439 - +2.2 592,439 - +1.3 592,448 - +1.5 592,448 - +0.8 592,449 + +1.2 592,482 + +1.8 592,504 - +1.9 592,517 - -0.2 592,597 - MMJJ_RS03135 0.27 -1.1 592,660 - MMJJ_RS03135 0.42 +1.4 592,660 - MMJJ_RS03135 0.42 +3.3 592,661 + MMJJ_RS03135 0.43 +2.2 592,665 - MMJJ_RS03135 0.43 +1.9 592,672 - MMJJ_RS03135 0.45 +2.2 592,673 + MMJJ_RS03135 0.45 +1.8 592,673 + MMJJ_RS03135 0.45 +1.7 592,688 - MMJJ_RS03135 0.49 +1.4 592,688 - MMJJ_RS03135 0.49 +0.4 592,693 - MMJJ_RS03135 0.50 +1.7 592,868 - +1.2 592,891 - +2.0 592,892 + +0.5 592,892 + +1.9 592,910 - +0.5 592,911 + +1.6 592,921 - -2.4 592,921 - -1.1 592,921 - -0.5 592,922 + +0.5 592,935 + +2.0 592,940 + +4.2 592,949 - +3.3 593,079 + MMJJ_RS03140 0.22 +2.7 593,105 + MMJJ_RS03140 0.25 +2.8 593,120 - MMJJ_RS03140 0.27 +1.5 593,120 - MMJJ_RS03140 0.27 +2.4 593,134 + MMJJ_RS03140 0.29 +2.9 593,135 - MMJJ_RS03140 0.29 +3.4 593,135 - MMJJ_RS03140 0.29 +3.1 593,136 + MMJJ_RS03140 0.29 +3.2 593,136 + MMJJ_RS03140 0.29 -1.8 593,136 + MMJJ_RS03140 0.29 +2.5 593,140 - MMJJ_RS03140 0.29 +0.5 593,253 + MMJJ_RS03140 0.44 +2.5 593,253 + MMJJ_RS03140 0.44 +2.1 593,307 + MMJJ_RS03140 0.51 +1.1
Or see this region's nucleotide sequence