Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02865 and MMJJ_RS02870 are separated by 115 nucleotides MMJJ_RS02870 and MMJJ_RS02875 are separated by 127 nucleotides MMJJ_RS02875 and MMJJ_RS02880 are separated by 42 nucleotides MMJJ_RS02880 and MMJJ_RS02885 are separated by 178 nucleotides MMJJ_RS02885 and MMJJ_RS02890 are separated by 9 nucleotides
MMJJ_RS02865: MMJJ_RS02865 - amino acid ABC transporter substrate-binding protein, at 540,484 to 541,230
_RS02865
MMJJ_RS02870: MMJJ_RS02870 - hypothetical protein, at 541,346 to 541,867
_RS02870
MMJJ_RS02875: MMJJ_RS02875 - site-2 protease family protein, at 541,995 to 543,008
_RS02875
MMJJ_RS02880: MMJJ_RS02880 - hypothetical protein, at 543,051 to 543,323
_RS02880
MMJJ_RS02885: MMJJ_RS02885 - NifB/NifX family molybdenum-iron cluster-binding protein, at 543,502 to 543,861
_RS02885
MMJJ_RS02890: MMJJ_RS02890 - ATP-binding protein, at 543,871 to 544,728
_RS02890
Position (kb) 541
542
543
544 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.001 kb on + strand, within MMJJ_RS02865 at 541.069 kb on - strand, within MMJJ_RS02865 at 541.089 kb on + strand, within MMJJ_RS02865 at 541.089 kb on + strand, within MMJJ_RS02865 at 541.096 kb on + strand, within MMJJ_RS02865 at 541.096 kb on + strand, within MMJJ_RS02865 at 541.128 kb on + strand, within MMJJ_RS02865 at 541.149 kb on + strand, within MMJJ_RS02865 at 541.196 kb on + strand at 541.228 kb on - strand at 541.260 kb on - strand at 541.261 kb on + strand at 541.264 kb on + strand at 541.264 kb on + strand at 541.264 kb on + strand at 541.278 kb on + strand at 541.278 kb on + strand at 541.358 kb on - strand at 541.367 kb on - strand at 541.368 kb on + strand at 541.368 kb on + strand at 541.372 kb on - strand at 541.373 kb on + strand at 541.436 kb on + strand, within MMJJ_RS02870 at 541.454 kb on - strand, within MMJJ_RS02870 at 541.455 kb on + strand, within MMJJ_RS02870 at 541.518 kb on - strand, within MMJJ_RS02870 at 541.518 kb on - strand, within MMJJ_RS02870 at 541.565 kb on + strand, within MMJJ_RS02870 at 541.566 kb on - strand, within MMJJ_RS02870 at 541.567 kb on + strand, within MMJJ_RS02870 at 541.629 kb on + strand, within MMJJ_RS02870 at 541.720 kb on - strand, within MMJJ_RS02870 at 541.748 kb on - strand, within MMJJ_RS02870 at 541.751 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.793 kb on + strand, within MMJJ_RS02870 at 541.827 kb on - strand at 541.833 kb on - strand at 541.842 kb on - strand at 541.842 kb on - strand at 541.848 kb on + strand at 541.877 kb on - strand at 541.882 kb on - strand at 541.882 kb on - strand at 541.883 kb on + strand at 541.904 kb on + strand at 541.918 kb on - strand at 541.919 kb on + strand at 541.919 kb on + strand at 541.922 kb on - strand at 541.922 kb on - strand at 541.922 kb on - strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.926 kb on - strand at 541.926 kb on - strand at 541.927 kb on + strand at 541.927 kb on + strand at 541.927 kb on + strand at 541.943 kb on + strand at 541.946 kb on + strand at 541.948 kb on - strand at 541.948 kb on - strand at 541.949 kb on + strand at 541.952 kb on - strand at 541.953 kb on + strand at 541.965 kb on - strand at 541.965 kb on - strand at 541.965 kb on - strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.967 kb on - strand at 541.967 kb on - strand at 541.967 kb on - strand at 541.968 kb on + strand at 541.968 kb on + strand at 541.970 kb on - strand at 541.970 kb on - strand at 541.970 kb on - strand at 541.970 kb on - strand at 541.971 kb on + strand at 541.971 kb on + strand at 541.971 kb on + strand at 541.971 kb on + strand at 541.971 kb on + strand at 542.045 kb on - strand at 542.048 kb on - strand at 542.132 kb on + strand, within MMJJ_RS02875 at 542.157 kb on - strand, within MMJJ_RS02875 at 542.157 kb on - strand, within MMJJ_RS02875 at 542.158 kb on + strand, within MMJJ_RS02875 at 542.158 kb on + strand, within MMJJ_RS02875 at 542.179 kb on - strand, within MMJJ_RS02875 at 542.302 kb on - strand, within MMJJ_RS02875 at 542.333 kb on - strand, within MMJJ_RS02875 at 542.381 kb on + strand, within MMJJ_RS02875 at 542.396 kb on - strand, within MMJJ_RS02875 at 542.435 kb on + strand, within MMJJ_RS02875 at 542.438 kb on - strand, within MMJJ_RS02875 at 542.472 kb on - strand, within MMJJ_RS02875 at 542.475 kb on + strand, within MMJJ_RS02875 at 542.476 kb on - strand, within MMJJ_RS02875 at 542.477 kb on + strand, within MMJJ_RS02875 at 542.522 kb on + strand, within MMJJ_RS02875 at 542.522 kb on + strand, within MMJJ_RS02875 at 542.557 kb on - strand, within MMJJ_RS02875 at 542.558 kb on + strand, within MMJJ_RS02875 at 542.558 kb on + strand, within MMJJ_RS02875 at 542.570 kb on + strand, within MMJJ_RS02875 at 542.578 kb on - strand, within MMJJ_RS02875 at 542.578 kb on - strand, within MMJJ_RS02875 at 542.579 kb on + strand, within MMJJ_RS02875 at 542.582 kb on - strand, within MMJJ_RS02875 at 542.582 kb on - strand, within MMJJ_RS02875 at 542.583 kb on + strand, within MMJJ_RS02875 at 542.587 kb on + strand, within MMJJ_RS02875 at 542.627 kb on - strand, within MMJJ_RS02875 at 542.627 kb on - strand, within MMJJ_RS02875 at 542.633 kb on + strand, within MMJJ_RS02875 at 542.633 kb on + strand, within MMJJ_RS02875 at 542.633 kb on + strand, within MMJJ_RS02875 at 542.633 kb on + strand, within MMJJ_RS02875 at 542.657 kb on - strand, within MMJJ_RS02875 at 542.658 kb on + strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.660 kb on - strand, within MMJJ_RS02875 at 542.661 kb on + strand, within MMJJ_RS02875 at 542.661 kb on + strand, within MMJJ_RS02875 at 542.667 kb on + strand, within MMJJ_RS02875 at 542.667 kb on + strand, within MMJJ_RS02875 at 542.673 kb on + strand, within MMJJ_RS02875 at 542.680 kb on + strand, within MMJJ_RS02875 at 542.684 kb on + strand, within MMJJ_RS02875 at 542.711 kb on + strand, within MMJJ_RS02875 at 542.768 kb on - strand, within MMJJ_RS02875 at 542.783 kb on - strand, within MMJJ_RS02875 at 542.797 kb on - strand, within MMJJ_RS02875 at 542.804 kb on - strand, within MMJJ_RS02875 at 542.805 kb on + strand, within MMJJ_RS02875 at 542.805 kb on + strand, within MMJJ_RS02875 at 542.823 kb on + strand, within MMJJ_RS02875 at 542.836 kb on - strand, within MMJJ_RS02875 at 542.876 kb on + strand, within MMJJ_RS02875 at 542.885 kb on + strand, within MMJJ_RS02875 at 542.904 kb on + strand, within MMJJ_RS02875 at 542.921 kb on + strand at 542.948 kb on + strand at 542.963 kb on - strand at 542.963 kb on - strand at 542.968 kb on - strand at 542.968 kb on - strand at 542.968 kb on - strand at 542.992 kb on - strand at 543.025 kb on + strand at 543.029 kb on - strand at 543.029 kb on - strand at 543.030 kb on + strand at 543.091 kb on - strand, within MMJJ_RS02880 at 543.091 kb on - strand, within MMJJ_RS02880 at 543.096 kb on + strand, within MMJJ_RS02880 at 543.116 kb on - strand, within MMJJ_RS02880 at 543.116 kb on - strand, within MMJJ_RS02880 at 543.121 kb on - strand, within MMJJ_RS02880 at 543.121 kb on - strand, within MMJJ_RS02880 at 543.124 kb on - strand, within MMJJ_RS02880 at 543.132 kb on - strand, within MMJJ_RS02880 at 543.132 kb on - strand, within MMJJ_RS02880 at 543.161 kb on - strand, within MMJJ_RS02880 at 543.161 kb on - strand, within MMJJ_RS02880 at 543.162 kb on + strand, within MMJJ_RS02880 at 543.162 kb on + strand, within MMJJ_RS02880 at 543.169 kb on - strand, within MMJJ_RS02880 at 543.170 kb on + strand, within MMJJ_RS02880 at 543.172 kb on - strand, within MMJJ_RS02880 at 543.197 kb on + strand, within MMJJ_RS02880 at 543.199 kb on - strand, within MMJJ_RS02880 at 543.212 kb on + strand, within MMJJ_RS02880 at 543.212 kb on + strand, within MMJJ_RS02880 at 543.242 kb on - strand, within MMJJ_RS02880 at 543.242 kb on - strand, within MMJJ_RS02880 at 543.250 kb on + strand, within MMJJ_RS02880 at 543.256 kb on - strand, within MMJJ_RS02880 at 543.256 kb on - strand, within MMJJ_RS02880 at 543.259 kb on - strand, within MMJJ_RS02880 at 543.259 kb on - strand, within MMJJ_RS02880 at 543.260 kb on + strand, within MMJJ_RS02880 at 543.266 kb on + strand, within MMJJ_RS02880 at 543.266 kb on + strand, within MMJJ_RS02880 at 543.299 kb on - strand at 543.305 kb on + strand at 543.311 kb on + strand at 543.311 kb on + strand at 543.325 kb on - strand at 543.363 kb on - strand at 543.366 kb on + strand at 543.373 kb on - strand at 543.403 kb on + strand at 543.409 kb on + strand at 543.449 kb on + strand at 543.462 kb on - strand at 543.462 kb on - strand at 543.531 kb on - strand at 543.532 kb on + strand at 543.582 kb on + strand, within MMJJ_RS02885 at 543.625 kb on + strand, within MMJJ_RS02885 at 543.655 kb on + strand, within MMJJ_RS02885 at 543.695 kb on - strand, within MMJJ_RS02885 at 543.695 kb on - strand, within MMJJ_RS02885 at 543.696 kb on + strand, within MMJJ_RS02885 at 543.750 kb on - strand, within MMJJ_RS02885 at 543.753 kb on - strand, within MMJJ_RS02885 at 543.760 kb on - strand, within MMJJ_RS02885 at 543.855 kb on - strand at 543.855 kb on - strand at 543.855 kb on - strand at 543.856 kb on + strand at 543.856 kb on + strand at 543.856 kb on + strand at 543.920 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 541,000 - MMJJ_RS02865 0.69 +1.0 541,000 - MMJJ_RS02865 0.69 +0.8 541,000 - MMJJ_RS02865 0.69 +2.0 541,000 - MMJJ_RS02865 0.69 -0.5 541,001 + MMJJ_RS02865 0.69 -2.1 541,069 - MMJJ_RS02865 0.78 +0.1 541,089 + MMJJ_RS02865 0.81 +0.8 541,089 + MMJJ_RS02865 0.81 -4.4 541,096 + MMJJ_RS02865 0.82 -1.7 541,096 + MMJJ_RS02865 0.82 -0.3 541,128 + MMJJ_RS02865 0.86 -0.1 541,149 + MMJJ_RS02865 0.89 -4.8 541,196 + -1.2 541,228 - -1.5 541,260 - +0.2 541,261 + +1.7 541,264 + -0.1 541,264 + +0.6 541,264 + -1.7 541,278 + +1.4 541,278 + -0.4 541,358 - +0.3 541,367 - -0.5 541,368 + -3.3 541,368 + -0.7 541,372 - +1.0 541,373 + +1.3 541,436 + MMJJ_RS02870 0.17 -0.2 541,454 - MMJJ_RS02870 0.21 -0.3 541,455 + MMJJ_RS02870 0.21 +0.4 541,518 - MMJJ_RS02870 0.33 -2.2 541,518 - MMJJ_RS02870 0.33 -3.1 541,565 + MMJJ_RS02870 0.42 -3.5 541,566 - MMJJ_RS02870 0.42 -1.3 541,567 + MMJJ_RS02870 0.42 -0.8 541,629 + MMJJ_RS02870 0.54 -0.5 541,720 - MMJJ_RS02870 0.72 -3.0 541,748 - MMJJ_RS02870 0.77 +1.5 541,751 + MMJJ_RS02870 0.78 -4.0 541,762 + MMJJ_RS02870 0.80 +0.9 541,762 + MMJJ_RS02870 0.80 +1.0 541,762 + MMJJ_RS02870 0.80 +1.7 541,762 + MMJJ_RS02870 0.80 -0.8 541,793 + MMJJ_RS02870 0.86 +1.5 541,827 - -5.0 541,833 - -2.1 541,842 - -2.8 541,842 - +1.6 541,848 + -1.1 541,877 - +0.2 541,882 - -0.0 541,882 - +1.1 541,883 + -0.8 541,904 + -3.7 541,918 - -2.5 541,919 + -1.0 541,919 + +0.9 541,922 - +0.4 541,922 - -2.0 541,922 - +1.3 541,923 + +0.8 541,923 + -0.5 541,923 + +0.9 541,923 + -0.3 541,923 + -1.1 541,923 + -3.0 541,923 + -5.9 541,926 - -0.0 541,926 - -0.1 541,927 + +2.8 541,927 + +0.6 541,927 + -1.2 541,943 + +0.3 541,946 + -0.9 541,948 - -0.9 541,948 - -5.0 541,949 + -2.0 541,952 - -2.7 541,953 + -2.8 541,965 - -2.4 541,965 - -0.9 541,965 - -3.2 541,966 + -1.8 541,966 + -2.7 541,966 + -3.6 541,966 + -1.1 541,966 + -1.4 541,966 + -4.2 541,967 - -2.5 541,967 - -0.4 541,967 - -1.2 541,968 + +0.0 541,968 + -2.8 541,970 - -2.4 541,970 - -0.1 541,970 - -2.3 541,970 - -4.0 541,971 + -4.5 541,971 + -3.0 541,971 + -2.3 541,971 + -0.9 541,971 + -0.0 542,045 - -2.1 542,048 - -1.7 542,132 + MMJJ_RS02875 0.14 -1.2 542,157 - MMJJ_RS02875 0.16 -1.2 542,157 - MMJJ_RS02875 0.16 -1.9 542,158 + MMJJ_RS02875 0.16 -0.0 542,158 + MMJJ_RS02875 0.16 -1.5 542,179 - MMJJ_RS02875 0.18 +0.6 542,302 - MMJJ_RS02875 0.30 -2.8 542,333 - MMJJ_RS02875 0.33 -0.9 542,381 + MMJJ_RS02875 0.38 -0.9 542,396 - MMJJ_RS02875 0.40 -1.1 542,435 + MMJJ_RS02875 0.43 +0.6 542,438 - MMJJ_RS02875 0.44 -0.7 542,472 - MMJJ_RS02875 0.47 -0.3 542,475 + MMJJ_RS02875 0.47 -1.1 542,476 - MMJJ_RS02875 0.47 -3.2 542,477 + MMJJ_RS02875 0.48 -4.1 542,522 + MMJJ_RS02875 0.52 -2.0 542,522 + MMJJ_RS02875 0.52 -4.0 542,557 - MMJJ_RS02875 0.55 -3.5 542,558 + MMJJ_RS02875 0.56 -1.5 542,558 + MMJJ_RS02875 0.56 -0.7 542,570 + MMJJ_RS02875 0.57 -0.5 542,578 - MMJJ_RS02875 0.57 -4.2 542,578 - MMJJ_RS02875 0.57 +0.5 542,579 + MMJJ_RS02875 0.58 -2.9 542,582 - MMJJ_RS02875 0.58 -3.3 542,582 - MMJJ_RS02875 0.58 -1.0 542,583 + MMJJ_RS02875 0.58 -5.4 542,587 + MMJJ_RS02875 0.58 -2.6 542,627 - MMJJ_RS02875 0.62 -4.1 542,627 - MMJJ_RS02875 0.62 +0.4 542,633 + MMJJ_RS02875 0.63 -2.6 542,633 + MMJJ_RS02875 0.63 -0.5 542,633 + MMJJ_RS02875 0.63 +0.5 542,633 + MMJJ_RS02875 0.63 -4.2 542,657 - MMJJ_RS02875 0.65 -1.9 542,658 + MMJJ_RS02875 0.65 +0.1 542,660 - MMJJ_RS02875 0.66 -0.0 542,660 - MMJJ_RS02875 0.66 +0.6 542,660 - MMJJ_RS02875 0.66 -0.4 542,660 - MMJJ_RS02875 0.66 +1.1 542,660 - MMJJ_RS02875 0.66 -3.4 542,660 - MMJJ_RS02875 0.66 -0.5 542,660 - MMJJ_RS02875 0.66 -2.5 542,661 + MMJJ_RS02875 0.66 -0.1 542,661 + MMJJ_RS02875 0.66 -3.0 542,667 + MMJJ_RS02875 0.66 -2.2 542,667 + MMJJ_RS02875 0.66 -3.1 542,673 + MMJJ_RS02875 0.67 -4.5 542,680 + MMJJ_RS02875 0.68 -1.1 542,684 + MMJJ_RS02875 0.68 +0.1 542,711 + MMJJ_RS02875 0.71 -1.5 542,768 - MMJJ_RS02875 0.76 -4.2 542,783 - MMJJ_RS02875 0.78 +0.5 542,797 - MMJJ_RS02875 0.79 -3.4 542,804 - MMJJ_RS02875 0.80 -3.2 542,805 + MMJJ_RS02875 0.80 +0.3 542,805 + MMJJ_RS02875 0.80 +0.1 542,823 + MMJJ_RS02875 0.82 -2.5 542,836 - MMJJ_RS02875 0.83 -2.2 542,876 + MMJJ_RS02875 0.87 -3.6 542,885 + MMJJ_RS02875 0.88 -2.5 542,904 + MMJJ_RS02875 0.90 -2.9 542,921 + -1.5 542,948 + +0.8 542,963 - -2.9 542,963 - -2.7 542,968 - -1.3 542,968 - -1.4 542,968 - -0.3 542,992 - +0.8 543,025 + +0.3 543,029 - +0.9 543,029 - -1.0 543,030 + +0.9 543,091 - MMJJ_RS02880 0.15 +1.8 543,091 - MMJJ_RS02880 0.15 -1.7 543,096 + MMJJ_RS02880 0.16 +1.1 543,116 - MMJJ_RS02880 0.24 +1.3 543,116 - MMJJ_RS02880 0.24 +1.2 543,121 - MMJJ_RS02880 0.26 +1.2 543,121 - MMJJ_RS02880 0.26 -1.2 543,124 - MMJJ_RS02880 0.27 -0.6 543,132 - MMJJ_RS02880 0.30 +0.3 543,132 - MMJJ_RS02880 0.30 -0.5 543,161 - MMJJ_RS02880 0.40 -0.0 543,161 - MMJJ_RS02880 0.40 -2.0 543,162 + MMJJ_RS02880 0.41 -0.5 543,162 + MMJJ_RS02880 0.41 -1.1 543,169 - MMJJ_RS02880 0.43 -0.1 543,170 + MMJJ_RS02880 0.44 +0.9 543,172 - MMJJ_RS02880 0.44 -1.9 543,197 + MMJJ_RS02880 0.53 -0.9 543,199 - MMJJ_RS02880 0.54 +0.7 543,212 + MMJJ_RS02880 0.59 -1.8 543,212 + MMJJ_RS02880 0.59 +1.1 543,242 - MMJJ_RS02880 0.70 -3.4 543,242 - MMJJ_RS02880 0.70 +0.8 543,250 + MMJJ_RS02880 0.73 +1.9 543,256 - MMJJ_RS02880 0.75 -1.9 543,256 - MMJJ_RS02880 0.75 -0.9 543,259 - MMJJ_RS02880 0.76 -0.5 543,259 - MMJJ_RS02880 0.76 -1.2 543,260 + MMJJ_RS02880 0.77 -2.9 543,266 + MMJJ_RS02880 0.79 -0.5 543,266 + MMJJ_RS02880 0.79 -2.5 543,299 - -0.2 543,305 + -0.5 543,311 + -0.1 543,311 + +0.8 543,325 - +0.3 543,363 - -1.3 543,366 + +0.6 543,373 - +0.5 543,403 + +0.1 543,409 + -3.1 543,449 + +0.9 543,462 - -0.9 543,462 - -0.0 543,531 - -3.2 543,532 + -0.7 543,582 + MMJJ_RS02885 0.22 -1.4 543,625 + MMJJ_RS02885 0.34 +0.8 543,655 + MMJJ_RS02885 0.42 -1.5 543,695 - MMJJ_RS02885 0.54 +1.2 543,695 - MMJJ_RS02885 0.54 +2.6 543,696 + MMJJ_RS02885 0.54 -0.4 543,750 - MMJJ_RS02885 0.69 -3.9 543,753 - MMJJ_RS02885 0.70 -0.3 543,760 - MMJJ_RS02885 0.72 -0.3 543,855 - -1.5 543,855 - +0.4 543,855 - +2.3 543,856 + +0.6 543,856 + -0.7 543,856 + +0.5 543,920 - +0.1
Or see this region's nucleotide sequence