Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02845 and MMJJ_RS02850 are separated by 167 nucleotides MMJJ_RS02850 and MMJJ_RS02855 are separated by 113 nucleotides
MMJJ_RS02845: MMJJ_RS02845 - hypothetical protein, at 534,978 to 536,624
_RS02845
MMJJ_RS02850: MMJJ_RS02850 - hypothetical protein, at 536,792 to 536,989
_RS02850
MMJJ_RS02855: MMJJ_RS02855 - DEAD/DEAH box helicase, at 537,103 to 538,719
_RS02855
Position (kb) 537
538
539 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 536.129 kb on + strand, within MMJJ_RS02845 at 536.138 kb on - strand, within MMJJ_RS02845 at 536.199 kb on - strand, within MMJJ_RS02845 at 536.290 kb on + strand, within MMJJ_RS02845 at 536.309 kb on - strand, within MMJJ_RS02845 at 536.309 kb on - strand at 536.310 kb on + strand, within MMJJ_RS02845 at 536.336 kb on - strand, within MMJJ_RS02845 at 536.336 kb on - strand, within MMJJ_RS02845 at 536.337 kb on + strand, within MMJJ_RS02845 at 536.355 kb on + strand, within MMJJ_RS02845 at 536.376 kb on - strand, within MMJJ_RS02845 at 536.376 kb on - strand, within MMJJ_RS02845 at 536.376 kb on - strand, within MMJJ_RS02845 at 536.376 kb on - strand, within MMJJ_RS02845 at 536.376 kb on - strand, within MMJJ_RS02845 at 536.378 kb on - strand, within MMJJ_RS02845 at 536.455 kb on - strand, within MMJJ_RS02845 at 536.456 kb on + strand, within MMJJ_RS02845 at 536.456 kb on + strand, within MMJJ_RS02845 at 536.466 kb on - strand at 536.467 kb on + strand at 536.467 kb on + strand at 536.469 kb on + strand at 536.469 kb on + strand at 536.469 kb on + strand at 536.561 kb on - strand at 536.562 kb on + strand at 536.604 kb on + strand at 536.646 kb on - strand at 536.655 kb on - strand at 536.655 kb on - strand at 536.656 kb on + strand at 536.656 kb on + strand at 536.662 kb on - strand at 536.669 kb on - strand at 536.669 kb on - strand at 536.672 kb on + strand at 536.722 kb on - strand at 536.722 kb on - strand at 536.727 kb on + strand at 536.747 kb on - strand at 536.763 kb on - strand at 536.764 kb on + strand at 536.766 kb on + strand at 536.775 kb on + strand at 536.791 kb on - strand at 536.791 kb on - strand at 536.792 kb on + strand at 536.792 kb on + strand at 536.796 kb on - strand at 536.803 kb on + strand at 536.806 kb on + strand at 536.806 kb on + strand at 536.845 kb on - strand, within MMJJ_RS02850 at 536.845 kb on - strand, within MMJJ_RS02850 at 536.845 kb on - strand, within MMJJ_RS02850 at 536.845 kb on - strand, within MMJJ_RS02850 at 536.846 kb on + strand, within MMJJ_RS02850 at 536.853 kb on - strand, within MMJJ_RS02850 at 536.853 kb on - strand, within MMJJ_RS02850 at 536.853 kb on - strand, within MMJJ_RS02850 at 536.888 kb on + strand, within MMJJ_RS02850 at 536.950 kb on - strand, within MMJJ_RS02850 at 536.951 kb on + strand, within MMJJ_RS02850 at 537.010 kb on - strand at 537.011 kb on + strand at 537.027 kb on - strand at 537.084 kb on + strand at 537.084 kb on + strand at 537.104 kb on - strand at 537.105 kb on + strand at 537.193 kb on - strand at 537.199 kb on - strand at 537.199 kb on - strand at 537.220 kb on - strand at 537.309 kb on - strand, within MMJJ_RS02855 at 537.314 kb on + strand, within MMJJ_RS02855 at 537.314 kb on + strand, within MMJJ_RS02855 at 537.319 kb on - strand, within MMJJ_RS02855 at 537.320 kb on + strand, within MMJJ_RS02855 at 537.331 kb on - strand, within MMJJ_RS02855 at 537.331 kb on - strand, within MMJJ_RS02855 at 537.436 kb on - strand, within MMJJ_RS02855 at 537.437 kb on + strand, within MMJJ_RS02855 at 537.560 kb on + strand, within MMJJ_RS02855 at 537.597 kb on - strand, within MMJJ_RS02855 at 537.597 kb on - strand, within MMJJ_RS02855 at 537.603 kb on - strand, within MMJJ_RS02855 at 537.603 kb on - strand, within MMJJ_RS02855 at 537.656 kb on - strand, within MMJJ_RS02855 at 537.660 kb on - strand, within MMJJ_RS02855 at 538.028 kb on + strand, within MMJJ_RS02855 at 538.059 kb on + strand, within MMJJ_RS02855 at 538.086 kb on - strand, within MMJJ_RS02855 at 538.152 kb on - strand, within MMJJ_RS02855 at 538.153 kb on + strand, within MMJJ_RS02855 at 538.267 kb on - strand, within MMJJ_RS02855 at 538.274 kb on + strand, within MMJJ_RS02855 at 538.278 kb on + strand, within MMJJ_RS02855 at 538.297 kb on - strand, within MMJJ_RS02855 at 538.366 kb on - strand, within MMJJ_RS02855 at 538.367 kb on + strand, within MMJJ_RS02855 at 538.482 kb on - strand, within MMJJ_RS02855 at 538.569 kb on - strand at 538.593 kb on - strand at 538.618 kb on + strand at 538.679 kb on + strand at 538.723 kb on + strand at 538.830 kb on - strand at 538.900 kb on + strand at 538.900 kb on + strand at 538.943 kb on - strand at 538.950 kb on + strand at 538.953 kb on + strand at 538.957 kb on - strand at 538.957 kb on - strand at 538.965 kb on - strand at 538.975 kb on - strand at 538.976 kb on + strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.992 kb on - strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.993 kb on + strand at 538.994 kb on - strand at 538.995 kb on + strand at 538.995 kb on + strand at 538.995 kb on + strand at 538.998 kb on - strand at 538.999 kb on + strand at 539.001 kb on - strand at 539.001 kb on - strand at 539.025 kb on + strand at 539.028 kb on - strand at 539.028 kb on - strand at 539.055 kb on - strand at 539.059 kb on - strand at 539.059 kb on - strand at 539.059 kb on - strand at 539.059 kb on - strand at 539.059 kb on - strand at 539.060 kb on + strand at 539.060 kb on + strand at 539.060 kb on + strand at 539.108 kb on - strand at 539.116 kb on - strand at 539.116 kb on - strand at 539.118 kb on - strand at 539.118 kb on - strand at 539.118 kb on - strand at 539.118 kb on - strand at 539.119 kb on + strand at 539.119 kb on + strand at 539.139 kb on + strand at 539.140 kb on - strand at 539.141 kb on + strand at 539.210 kb on - strand at 539.210 kb on - strand at 539.211 kb on + strand at 539.211 kb on + strand at 539.221 kb on - strand at 539.226 kb on + strand at 539.226 kb on + strand at 539.226 kb on + strand at 539.229 kb on - strand at 539.230 kb on + strand at 539.230 kb on + strand at 539.230 kb on + strand at 539.233 kb on - strand at 539.234 kb on + strand at 539.243 kb on - strand at 539.244 kb on + strand at 539.244 kb on + strand at 539.244 kb on + strand at 539.244 kb on + strand at 539.244 kb on + strand at 539.254 kb on - strand at 539.264 kb on - strand at 539.265 kb on + strand at 539.265 kb on + strand at 539.265 kb on + strand at 539.265 kb on + strand at 539.265 kb on + strand at 539.266 kb on - strand at 539.266 kb on - strand at 539.266 kb on - strand at 539.267 kb on + strand at 539.267 kb on + strand at 539.267 kb on + strand at 539.268 kb on - strand at 539.268 kb on - strand at 539.268 kb on - strand at 539.268 kb on - strand at 539.269 kb on + strand at 539.269 kb on + strand at 539.269 kb on + strand at 539.269 kb on + strand at 539.273 kb on - strand at 539.274 kb on + strand at 539.278 kb on - strand at 539.323 kb on - strand at 539.337 kb on - strand at 539.337 kb on - strand at 539.367 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 536,129 + MMJJ_RS02845 0.70 -2.4 536,138 - MMJJ_RS02845 0.70 +0.7 536,199 - MMJJ_RS02845 0.74 +0.1 536,290 + MMJJ_RS02845 0.80 -1.0 536,309 - MMJJ_RS02845 0.81 -1.9 536,309 - +0.5 536,310 + MMJJ_RS02845 0.81 -0.5 536,336 - MMJJ_RS02845 0.82 +1.2 536,336 - MMJJ_RS02845 0.82 +0.3 536,337 + MMJJ_RS02845 0.83 +2.6 536,355 + MMJJ_RS02845 0.84 -1.4 536,376 - MMJJ_RS02845 0.85 +0.9 536,376 - MMJJ_RS02845 0.85 -3.2 536,376 - MMJJ_RS02845 0.85 -0.4 536,376 - MMJJ_RS02845 0.85 +0.7 536,376 - MMJJ_RS02845 0.85 -0.6 536,378 - MMJJ_RS02845 0.85 -1.5 536,455 - MMJJ_RS02845 0.90 -0.9 536,456 + MMJJ_RS02845 0.90 +0.0 536,456 + MMJJ_RS02845 0.90 -0.5 536,466 - -1.8 536,467 + +0.1 536,467 + +0.9 536,469 + -0.1 536,469 + -2.0 536,469 + -3.8 536,561 - +1.1 536,562 + +2.1 536,604 + +0.6 536,646 - +0.3 536,655 - +3.3 536,655 - +1.3 536,656 + +0.2 536,656 + -0.2 536,662 - +0.3 536,669 - -0.6 536,669 - -2.1 536,672 + +0.4 536,722 - +0.2 536,722 - -0.5 536,727 + +0.7 536,747 - +1.1 536,763 - -1.4 536,764 + -2.0 536,766 + -3.2 536,775 + +1.5 536,791 - -0.5 536,791 - +0.9 536,792 + -3.5 536,792 + +1.0 536,796 - +1.4 536,803 + -0.8 536,806 + +0.1 536,806 + +2.6 536,845 - MMJJ_RS02850 0.27 +1.2 536,845 - MMJJ_RS02850 0.27 +0.9 536,845 - MMJJ_RS02850 0.27 -0.6 536,845 - MMJJ_RS02850 0.27 +0.8 536,846 + MMJJ_RS02850 0.27 -0.2 536,853 - MMJJ_RS02850 0.31 +2.9 536,853 - MMJJ_RS02850 0.31 +3.6 536,853 - MMJJ_RS02850 0.31 -2.4 536,888 + MMJJ_RS02850 0.48 -1.5 536,950 - MMJJ_RS02850 0.80 +0.9 536,951 + MMJJ_RS02850 0.80 -3.4 537,010 - -1.2 537,011 + +0.4 537,027 - +0.4 537,084 + -1.1 537,084 + -0.5 537,104 - -3.8 537,105 + +1.3 537,193 - -2.5 537,199 - +2.9 537,199 - +0.5 537,220 - -3.8 537,309 - MMJJ_RS02855 0.13 -2.5 537,314 + MMJJ_RS02855 0.13 -1.4 537,314 + MMJJ_RS02855 0.13 -2.7 537,319 - MMJJ_RS02855 0.13 -0.9 537,320 + MMJJ_RS02855 0.13 -2.2 537,331 - MMJJ_RS02855 0.14 -1.5 537,331 - MMJJ_RS02855 0.14 -3.5 537,436 - MMJJ_RS02855 0.21 -3.4 537,437 + MMJJ_RS02855 0.21 -3.1 537,560 + MMJJ_RS02855 0.28 -2.5 537,597 - MMJJ_RS02855 0.31 -2.5 537,597 - MMJJ_RS02855 0.31 -4.3 537,603 - MMJJ_RS02855 0.31 -2.6 537,603 - MMJJ_RS02855 0.31 -3.1 537,656 - MMJJ_RS02855 0.34 -1.7 537,660 - MMJJ_RS02855 0.34 -4.1 538,028 + MMJJ_RS02855 0.57 -2.5 538,059 + MMJJ_RS02855 0.59 -1.1 538,086 - MMJJ_RS02855 0.61 -2.7 538,152 - MMJJ_RS02855 0.65 -1.1 538,153 + MMJJ_RS02855 0.65 -0.5 538,267 - MMJJ_RS02855 0.72 -2.1 538,274 + MMJJ_RS02855 0.72 -1.7 538,278 + MMJJ_RS02855 0.73 -3.2 538,297 - MMJJ_RS02855 0.74 -1.9 538,366 - MMJJ_RS02855 0.78 -0.5 538,367 + MMJJ_RS02855 0.78 -0.5 538,482 - MMJJ_RS02855 0.85 -1.9 538,569 - -2.2 538,593 - -0.1 538,618 + -3.1 538,679 + -1.9 538,723 + -0.5 538,830 - -0.9 538,900 + -2.7 538,900 + -2.9 538,943 - -0.9 538,950 + +1.5 538,953 + -3.1 538,957 - -3.2 538,957 - -0.1 538,965 - +0.5 538,975 - -0.8 538,976 + -1.5 538,992 - +1.2 538,992 - +1.5 538,992 - -0.3 538,992 - +2.3 538,992 - +0.4 538,992 - -0.1 538,992 - -1.2 538,992 - -2.9 538,993 + +2.7 538,993 + +0.8 538,993 + +1.3 538,993 + +0.6 538,993 + -0.7 538,993 + -0.2 538,993 + -1.2 538,994 - -0.9 538,995 + -2.0 538,995 + +0.3 538,995 + +1.6 538,998 - +0.7 538,999 + -3.2 539,001 - -0.4 539,001 - +2.8 539,025 + +0.0 539,028 - +2.0 539,028 - -2.6 539,055 - +0.2 539,059 - -2.4 539,059 - -0.1 539,059 - +0.0 539,059 - -2.8 539,059 - +0.2 539,060 + -1.8 539,060 + -2.5 539,060 + -1.1 539,108 - -0.4 539,116 - +0.1 539,116 - -3.2 539,118 - +0.0 539,118 - -1.2 539,118 - +1.8 539,118 - +2.3 539,119 + -0.2 539,119 + -0.8 539,139 + +0.8 539,140 - -2.1 539,141 + +0.8 539,210 - -2.9 539,210 - +1.0 539,211 + -0.1 539,211 + -1.5 539,221 - +2.1 539,226 + +0.0 539,226 + -1.8 539,226 + -3.6 539,229 - -0.4 539,230 + -0.5 539,230 + -4.2 539,230 + -0.1 539,233 - +0.0 539,234 + -1.5 539,243 - +1.3 539,244 + +0.7 539,244 + +3.5 539,244 + -0.0 539,244 + +0.1 539,244 + +1.1 539,254 - -2.4 539,264 - +0.1 539,265 + -3.3 539,265 + -3.3 539,265 + +1.0 539,265 + +0.4 539,265 + +0.6 539,266 - -1.1 539,266 - +0.2 539,266 - +0.7 539,267 + +0.0 539,267 + -0.1 539,267 + -0.6 539,268 - +1.2 539,268 - +0.4 539,268 - -0.1 539,268 - +1.6 539,269 + +1.4 539,269 + -0.5 539,269 + +0.5 539,269 + -3.2 539,273 - -3.3 539,274 + +0.4 539,278 - -0.7 539,323 - +1.1 539,337 - +1.4 539,337 - +0.8 539,367 - -1.5
Or see this region's nucleotide sequence