Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02360 and MMJJ_RS02365 are separated by 26 nucleotides MMJJ_RS02365 and MMJJ_RS02375 are separated by 180 nucleotides MMJJ_RS02375 and MMJJ_RS02380 are separated by 9 nucleotides MMJJ_RS02380 and MMJJ_RS02385 are separated by 11 nucleotides
MMJJ_RS02360: MMJJ_RS02360 - hypothetical protein, at 447,713 to 448,192
_RS02360
MMJJ_RS02365: MMJJ_RS02365 - DUF2540 domain-containing protein, at 448,219 to 448,464
_RS02365
MMJJ_RS02375: MMJJ_RS02375 - site-specific integrase, at 448,645 to 449,469
_RS02375
MMJJ_RS02380: MMJJ_RS02380 - hypothetical protein, at 449,479 to 450,147
_RS02380
MMJJ_RS02385: MMJJ_RS02385 - hypothetical protein, at 450,159 to 451,604
_RS02385
Position (kb) 448
449
450 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 447.649 kb on - strand at 447.649 kb on - strand at 447.650 kb on + strand at 447.675 kb on - strand at 447.675 kb on - strand at 447.678 kb on - strand at 447.678 kb on - strand at 447.679 kb on + strand at 447.679 kb on + strand at 447.679 kb on + strand at 447.685 kb on + strand at 447.697 kb on - strand at 447.697 kb on - strand at 447.822 kb on + strand, within MMJJ_RS02360 at 447.834 kb on + strand, within MMJJ_RS02360 at 447.834 kb on + strand, within MMJJ_RS02360 at 447.878 kb on + strand, within MMJJ_RS02360 at 447.883 kb on + strand, within MMJJ_RS02360 at 447.898 kb on - strand, within MMJJ_RS02360 at 447.898 kb on - strand, within MMJJ_RS02360 at 447.960 kb on + strand, within MMJJ_RS02360 at 447.979 kb on - strand, within MMJJ_RS02360 at 447.993 kb on - strand, within MMJJ_RS02360 at 448.065 kb on - strand, within MMJJ_RS02360 at 448.106 kb on + strand, within MMJJ_RS02360 at 448.163 kb on + strand at 448.171 kb on + strand at 448.190 kb on - strand at 448.191 kb on + strand at 448.209 kb on + strand at 448.245 kb on + strand, within MMJJ_RS02365 at 448.277 kb on + strand, within MMJJ_RS02365 at 448.277 kb on + strand, within MMJJ_RS02365 at 448.305 kb on - strand, within MMJJ_RS02365 at 448.306 kb on + strand, within MMJJ_RS02365 at 448.306 kb on + strand, within MMJJ_RS02365 at 448.433 kb on + strand, within MMJJ_RS02365 at 448.436 kb on - strand, within MMJJ_RS02365 at 448.437 kb on + strand, within MMJJ_RS02365 at 448.437 kb on + strand, within MMJJ_RS02365 at 448.713 kb on - strand at 448.713 kb on - strand at 448.725 kb on - strand at 448.725 kb on - strand at 448.761 kb on - strand, within MMJJ_RS02375 at 448.762 kb on + strand, within MMJJ_RS02375 at 448.789 kb on + strand, within MMJJ_RS02375 at 448.789 kb on + strand, within MMJJ_RS02375 at 448.791 kb on - strand, within MMJJ_RS02375 at 448.894 kb on - strand, within MMJJ_RS02375 at 448.894 kb on - strand, within MMJJ_RS02375 at 448.895 kb on + strand, within MMJJ_RS02375 at 448.905 kb on + strand, within MMJJ_RS02375 at 448.919 kb on - strand, within MMJJ_RS02375 at 448.920 kb on + strand, within MMJJ_RS02375 at 448.937 kb on - strand, within MMJJ_RS02375 at 448.938 kb on + strand, within MMJJ_RS02375 at 448.938 kb on + strand, within MMJJ_RS02375 at 448.938 kb on + strand, within MMJJ_RS02375 at 448.950 kb on - strand, within MMJJ_RS02375 at 448.951 kb on + strand, within MMJJ_RS02375 at 449.033 kb on - strand, within MMJJ_RS02375 at 449.034 kb on + strand, within MMJJ_RS02375 at 449.034 kb on + strand, within MMJJ_RS02375 at 449.075 kb on - strand, within MMJJ_RS02375 at 449.091 kb on - strand, within MMJJ_RS02375 at 449.111 kb on - strand, within MMJJ_RS02375 at 449.111 kb on - strand, within MMJJ_RS02375 at 449.130 kb on + strand, within MMJJ_RS02375 at 449.139 kb on + strand, within MMJJ_RS02375 at 449.143 kb on - strand, within MMJJ_RS02375 at 449.144 kb on + strand, within MMJJ_RS02375 at 449.144 kb on + strand, within MMJJ_RS02375 at 449.144 kb on + strand, within MMJJ_RS02375 at 449.144 kb on + strand, within MMJJ_RS02375 at 449.144 kb on + strand, within MMJJ_RS02375 at 449.146 kb on - strand, within MMJJ_RS02375 at 449.146 kb on - strand, within MMJJ_RS02375 at 449.155 kb on - strand, within MMJJ_RS02375 at 449.155 kb on - strand, within MMJJ_RS02375 at 449.155 kb on - strand, within MMJJ_RS02375 at 449.175 kb on - strand, within MMJJ_RS02375 at 449.176 kb on + strand, within MMJJ_RS02375 at 449.176 kb on + strand, within MMJJ_RS02375 at 449.176 kb on + strand, within MMJJ_RS02375 at 449.237 kb on - strand, within MMJJ_RS02375 at 449.313 kb on + strand, within MMJJ_RS02375 at 449.337 kb on + strand, within MMJJ_RS02375 at 449.358 kb on + strand, within MMJJ_RS02375 at 449.362 kb on + strand, within MMJJ_RS02375 at 449.366 kb on - strand, within MMJJ_RS02375 at 449.532 kb on - strand at 449.539 kb on - strand at 449.539 kb on - strand at 449.540 kb on + strand at 449.560 kb on - strand, within MMJJ_RS02380 at 449.560 kb on - strand, within MMJJ_RS02380 at 449.561 kb on + strand, within MMJJ_RS02380 at 449.566 kb on + strand, within MMJJ_RS02380 at 449.568 kb on + strand, within MMJJ_RS02380 at 449.606 kb on - strand, within MMJJ_RS02380 at 449.606 kb on - strand, within MMJJ_RS02380 at 449.607 kb on + strand, within MMJJ_RS02380 at 449.607 kb on + strand, within MMJJ_RS02380 at 449.650 kb on + strand, within MMJJ_RS02380 at 449.673 kb on - strand, within MMJJ_RS02380 at 449.673 kb on - strand, within MMJJ_RS02380 at 449.673 kb on - strand, within MMJJ_RS02380 at 449.674 kb on + strand, within MMJJ_RS02380 at 449.677 kb on - strand, within MMJJ_RS02380 at 449.677 kb on - strand, within MMJJ_RS02380 at 449.677 kb on - strand, within MMJJ_RS02380 at 449.677 kb on - strand, within MMJJ_RS02380 at 449.677 kb on - strand, within MMJJ_RS02380 at 449.677 kb on - strand, within MMJJ_RS02380 at 449.678 kb on + strand, within MMJJ_RS02380 at 449.678 kb on + strand, within MMJJ_RS02380 at 449.678 kb on + strand, within MMJJ_RS02380 at 449.678 kb on + strand, within MMJJ_RS02380 at 449.728 kb on - strand, within MMJJ_RS02380 at 449.729 kb on + strand, within MMJJ_RS02380 at 449.759 kb on - strand, within MMJJ_RS02380 at 449.759 kb on - strand, within MMJJ_RS02380 at 449.760 kb on + strand, within MMJJ_RS02380 at 449.760 kb on + strand, within MMJJ_RS02380 at 449.760 kb on + strand, within MMJJ_RS02380 at 449.763 kb on - strand, within MMJJ_RS02380 at 449.763 kb on - strand, within MMJJ_RS02380 at 449.763 kb on - strand, within MMJJ_RS02380 at 449.763 kb on - strand, within MMJJ_RS02380 at 449.763 kb on - strand, within MMJJ_RS02380 at 449.764 kb on + strand, within MMJJ_RS02380 at 449.764 kb on + strand at 449.764 kb on + strand, within MMJJ_RS02380 at 449.764 kb on + strand, within MMJJ_RS02380 at 449.764 kb on + strand, within MMJJ_RS02380 at 449.764 kb on + strand, within MMJJ_RS02380 at 449.764 kb on + strand at 449.802 kb on - strand, within MMJJ_RS02380 at 449.880 kb on - strand, within MMJJ_RS02380 at 449.880 kb on - strand, within MMJJ_RS02380 at 449.922 kb on + strand, within MMJJ_RS02380 at 449.928 kb on - strand, within MMJJ_RS02380 at 449.957 kb on - strand, within MMJJ_RS02380 at 449.958 kb on + strand, within MMJJ_RS02380 at 449.958 kb on + strand, within MMJJ_RS02380 at 449.958 kb on + strand, within MMJJ_RS02380 at 449.973 kb on - strand, within MMJJ_RS02380 at 449.996 kb on - strand, within MMJJ_RS02380 at 449.997 kb on + strand, within MMJJ_RS02380 at 450.011 kb on - strand, within MMJJ_RS02380 at 450.011 kb on - strand, within MMJJ_RS02380 at 450.012 kb on + strand, within MMJJ_RS02380 at 450.046 kb on + strand, within MMJJ_RS02380 at 450.047 kb on - strand, within MMJJ_RS02380 at 450.069 kb on + strand, within MMJJ_RS02380 at 450.098 kb on - strand at 450.153 kb on - strand at 450.154 kb on + strand at 450.154 kb on + strand at 450.154 kb on + strand at 450.158 kb on + strand at 450.161 kb on + strand at 450.170 kb on - strand at 450.170 kb on - strand at 450.191 kb on - strand at 450.192 kb on + strand at 450.192 kb on + strand at 450.192 kb on + strand at 450.192 kb on + strand at 450.193 kb on - strand at 450.193 kb on - strand at 450.193 kb on - strand at 450.193 kb on - strand at 450.193 kb on - strand at 450.194 kb on + strand at 450.194 kb on + strand at 450.194 kb on + strand at 450.194 kb on + strand at 450.194 kb on + strand at 450.194 kb on + strand at 450.194 kb on + strand at 450.198 kb on + strand at 450.198 kb on + strand at 450.227 kb on - strand at 450.228 kb on + strand at 450.228 kb on + strand at 450.228 kb on + strand at 450.228 kb on + strand at 450.234 kb on + strand at 450.237 kb on - strand at 450.238 kb on + strand at 450.246 kb on + strand at 450.250 kb on + strand at 450.276 kb on - strand at 450.302 kb on - strand at 450.304 kb on - strand, within MMJJ_RS02385 at 450.304 kb on - strand, within MMJJ_RS02385 at 450.305 kb on + strand, within MMJJ_RS02385 at 450.305 kb on + strand, within MMJJ_RS02385 at 450.323 kb on - strand, within MMJJ_RS02385 at 450.353 kb on + strand, within MMJJ_RS02385 at 450.353 kb on + strand, within MMJJ_RS02385 at 450.375 kb on - strand, within MMJJ_RS02385 at 450.375 kb on - strand, within MMJJ_RS02385 at 450.389 kb on - strand, within MMJJ_RS02385 at 450.410 kb on - strand, within MMJJ_RS02385 at 450.441 kb on - strand, within MMJJ_RS02385
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 447,649 - -2.2 447,649 - +1.3 447,650 + +1.7 447,675 - +1.0 447,675 - -1.8 447,678 - -1.1 447,678 - +0.1 447,679 + -2.9 447,679 + +0.1 447,679 + -3.5 447,685 + -3.0 447,697 - +0.8 447,697 - -1.0 447,822 + MMJJ_RS02360 0.23 -0.9 447,834 + MMJJ_RS02360 0.25 -0.1 447,834 + MMJJ_RS02360 0.25 -2.2 447,878 + MMJJ_RS02360 0.34 +0.9 447,883 + MMJJ_RS02360 0.35 +0.2 447,898 - MMJJ_RS02360 0.39 -0.1 447,898 - MMJJ_RS02360 0.39 +0.4 447,960 + MMJJ_RS02360 0.51 +0.0 447,979 - MMJJ_RS02360 0.55 -4.6 447,993 - MMJJ_RS02360 0.58 -0.5 448,065 - MMJJ_RS02360 0.73 +1.5 448,106 + MMJJ_RS02360 0.82 +1.3 448,163 + -4.3 448,171 + -2.7 448,190 - +0.5 448,191 + +0.4 448,209 + +1.5 448,245 + MMJJ_RS02365 0.11 +0.8 448,277 + MMJJ_RS02365 0.24 -0.6 448,277 + MMJJ_RS02365 0.24 +0.8 448,305 - MMJJ_RS02365 0.35 +1.4 448,306 + MMJJ_RS02365 0.35 -0.7 448,306 + MMJJ_RS02365 0.35 +1.4 448,433 + MMJJ_RS02365 0.87 -0.5 448,436 - MMJJ_RS02365 0.88 -0.8 448,437 + MMJJ_RS02365 0.89 +0.9 448,437 + MMJJ_RS02365 0.89 -0.2 448,713 - +0.4 448,713 - -0.9 448,725 - -2.5 448,725 - +0.6 448,761 - MMJJ_RS02375 0.14 -0.9 448,762 + MMJJ_RS02375 0.14 +1.9 448,789 + MMJJ_RS02375 0.17 +0.5 448,789 + MMJJ_RS02375 0.17 -1.7 448,791 - MMJJ_RS02375 0.18 -0.9 448,894 - MMJJ_RS02375 0.30 +0.6 448,894 - MMJJ_RS02375 0.30 +0.4 448,895 + MMJJ_RS02375 0.30 -0.5 448,905 + MMJJ_RS02375 0.32 -1.6 448,919 - MMJJ_RS02375 0.33 -0.4 448,920 + MMJJ_RS02375 0.33 +0.9 448,937 - MMJJ_RS02375 0.35 +0.8 448,938 + MMJJ_RS02375 0.36 -0.5 448,938 + MMJJ_RS02375 0.36 -0.1 448,938 + MMJJ_RS02375 0.36 +2.3 448,950 - MMJJ_RS02375 0.37 +1.3 448,951 + MMJJ_RS02375 0.37 -3.3 449,033 - MMJJ_RS02375 0.47 +1.1 449,034 + MMJJ_RS02375 0.47 +0.1 449,034 + MMJJ_RS02375 0.47 +0.4 449,075 - MMJJ_RS02375 0.52 -2.3 449,091 - MMJJ_RS02375 0.54 +1.5 449,111 - MMJJ_RS02375 0.56 +0.4 449,111 - MMJJ_RS02375 0.56 +0.5 449,130 + MMJJ_RS02375 0.59 +0.4 449,139 + MMJJ_RS02375 0.60 +1.0 449,143 - MMJJ_RS02375 0.60 -0.7 449,144 + MMJJ_RS02375 0.60 +1.1 449,144 + MMJJ_RS02375 0.60 -0.5 449,144 + MMJJ_RS02375 0.60 -1.0 449,144 + MMJJ_RS02375 0.60 +1.6 449,144 + MMJJ_RS02375 0.60 -1.2 449,146 - MMJJ_RS02375 0.61 -2.4 449,146 - MMJJ_RS02375 0.61 +1.1 449,155 - MMJJ_RS02375 0.62 +0.0 449,155 - MMJJ_RS02375 0.62 -0.2 449,155 - MMJJ_RS02375 0.62 +0.3 449,175 - MMJJ_RS02375 0.64 +0.2 449,176 + MMJJ_RS02375 0.64 +2.0 449,176 + MMJJ_RS02375 0.64 -4.0 449,176 + MMJJ_RS02375 0.64 +0.4 449,237 - MMJJ_RS02375 0.72 +1.0 449,313 + MMJJ_RS02375 0.81 +0.3 449,337 + MMJJ_RS02375 0.84 +1.5 449,358 + MMJJ_RS02375 0.86 +1.4 449,362 + MMJJ_RS02375 0.87 +2.1 449,366 - MMJJ_RS02375 0.87 -1.9 449,532 - +0.3 449,539 - -1.7 449,539 - +1.2 449,540 + +0.9 449,560 - MMJJ_RS02380 0.12 -1.9 449,560 - MMJJ_RS02380 0.12 +0.4 449,561 + MMJJ_RS02380 0.12 -0.7 449,566 + MMJJ_RS02380 0.13 -2.7 449,568 + MMJJ_RS02380 0.13 +0.0 449,606 - MMJJ_RS02380 0.19 -1.3 449,606 - MMJJ_RS02380 0.19 +0.1 449,607 + MMJJ_RS02380 0.19 -0.9 449,607 + MMJJ_RS02380 0.19 -3.4 449,650 + MMJJ_RS02380 0.26 +2.4 449,673 - MMJJ_RS02380 0.29 +2.4 449,673 - MMJJ_RS02380 0.29 -0.0 449,673 - MMJJ_RS02380 0.29 -1.3 449,674 + MMJJ_RS02380 0.29 +2.0 449,677 - MMJJ_RS02380 0.30 -0.3 449,677 - MMJJ_RS02380 0.30 -0.6 449,677 - MMJJ_RS02380 0.30 -0.3 449,677 - MMJJ_RS02380 0.30 +1.5 449,677 - MMJJ_RS02380 0.30 -1.7 449,677 - MMJJ_RS02380 0.30 -0.5 449,678 + MMJJ_RS02380 0.30 +1.5 449,678 + MMJJ_RS02380 0.30 -0.1 449,678 + MMJJ_RS02380 0.30 -0.8 449,678 + MMJJ_RS02380 0.30 -1.7 449,728 - MMJJ_RS02380 0.37 -1.4 449,729 + MMJJ_RS02380 0.37 -1.2 449,759 - MMJJ_RS02380 0.42 +0.0 449,759 - MMJJ_RS02380 0.42 +1.4 449,760 + MMJJ_RS02380 0.42 -0.7 449,760 + MMJJ_RS02380 0.42 -0.4 449,760 + MMJJ_RS02380 0.42 +0.3 449,763 - MMJJ_RS02380 0.42 -0.8 449,763 - MMJJ_RS02380 0.42 +0.6 449,763 - MMJJ_RS02380 0.42 +0.5 449,763 - MMJJ_RS02380 0.42 +0.5 449,763 - MMJJ_RS02380 0.42 -1.6 449,764 + MMJJ_RS02380 0.43 -1.5 449,764 + -0.1 449,764 + MMJJ_RS02380 0.43 -0.2 449,764 + MMJJ_RS02380 0.43 -2.8 449,764 + MMJJ_RS02380 0.43 +0.2 449,764 + MMJJ_RS02380 0.43 -1.7 449,764 + -0.1 449,802 - MMJJ_RS02380 0.48 +1.7 449,880 - MMJJ_RS02380 0.60 +1.4 449,880 - MMJJ_RS02380 0.60 -0.7 449,922 + MMJJ_RS02380 0.66 -0.3 449,928 - MMJJ_RS02380 0.67 +1.2 449,957 - MMJJ_RS02380 0.71 +1.5 449,958 + MMJJ_RS02380 0.72 +1.0 449,958 + MMJJ_RS02380 0.72 +0.8 449,958 + MMJJ_RS02380 0.72 -2.9 449,973 - MMJJ_RS02380 0.74 -2.5 449,996 - MMJJ_RS02380 0.77 +2.8 449,997 + MMJJ_RS02380 0.77 +0.9 450,011 - MMJJ_RS02380 0.80 -0.7 450,011 - MMJJ_RS02380 0.80 +1.4 450,012 + MMJJ_RS02380 0.80 -0.1 450,046 + MMJJ_RS02380 0.85 +0.9 450,047 - MMJJ_RS02380 0.85 +1.1 450,069 + MMJJ_RS02380 0.88 -1.2 450,098 - +0.8 450,153 - +0.0 450,154 + +1.4 450,154 + +1.9 450,154 + +2.6 450,158 + +0.8 450,161 + -1.3 450,170 - +0.4 450,170 - +0.9 450,191 - -2.9 450,192 + +0.3 450,192 + +0.1 450,192 + -4.5 450,192 + -5.1 450,193 - +1.4 450,193 - +1.6 450,193 - -0.4 450,193 - -0.0 450,193 - -0.4 450,194 + +0.1 450,194 + +0.9 450,194 + -3.1 450,194 + +0.4 450,194 + -0.6 450,194 + +0.9 450,194 + -4.0 450,198 + -2.4 450,198 + +1.4 450,227 - -1.4 450,228 + +0.1 450,228 + -1.6 450,228 + -2.2 450,228 + +0.3 450,234 + -1.4 450,237 - -1.1 450,238 + -0.7 450,246 + -0.2 450,250 + +0.7 450,276 - -0.8 450,302 - +1.4 450,304 - MMJJ_RS02385 0.10 +1.1 450,304 - MMJJ_RS02385 0.10 +1.1 450,305 + MMJJ_RS02385 0.10 +0.4 450,305 + MMJJ_RS02385 0.10 -0.8 450,323 - MMJJ_RS02385 0.11 -0.7 450,353 + MMJJ_RS02385 0.13 -2.0 450,353 + MMJJ_RS02385 0.13 +1.0 450,375 - MMJJ_RS02385 0.15 -0.5 450,375 - MMJJ_RS02385 0.15 +1.4 450,389 - MMJJ_RS02385 0.16 +2.1 450,410 - MMJJ_RS02385 0.17 +0.9 450,441 - MMJJ_RS02385 0.20 -1.3
Or see this region's nucleotide sequence