Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01970 and MMJJ_RS01975 are separated by 5 nucleotides MMJJ_RS01975 and MMJJ_RS01980 are separated by 116 nucleotides MMJJ_RS01980 and MMJJ_RS01985 are separated by 26 nucleotides MMJJ_RS01985 and MMJJ_RS01990 are separated by 4 nucleotides
MMJJ_RS01970: MMJJ_RS01970 - hypothetical protein, at 377,315 to 377,515
_RS01970
MMJJ_RS01975: MMJJ_RS01975 - diphthine synthase, at 377,521 to 378,288
_RS01975
MMJJ_RS01980: MMJJ_RS01980 - cation:proton antiporter, at 378,405 to 379,574
_RS01980
MMJJ_RS01985: MMJJ_RS01985 - endonuclease III, at 379,601 to 380,299
_RS01985
MMJJ_RS01990: MMJJ_RS01990 - universal stress protein, at 380,304 to 380,741
_RS01990
Position (kb) 378
379
380 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 377.415 kb on - strand, within MMJJ_RS01970 at 377.616 kb on + strand, within MMJJ_RS01975 at 377.645 kb on - strand, within MMJJ_RS01975 at 377.659 kb on + strand, within MMJJ_RS01975 at 377.661 kb on - strand, within MMJJ_RS01975 at 377.662 kb on + strand, within MMJJ_RS01975 at 377.662 kb on + strand, within MMJJ_RS01975 at 377.677 kb on - strand, within MMJJ_RS01975 at 377.794 kb on + strand, within MMJJ_RS01975 at 377.798 kb on + strand, within MMJJ_RS01975 at 377.841 kb on - strand, within MMJJ_RS01975 at 377.884 kb on - strand, within MMJJ_RS01975 at 377.885 kb on + strand, within MMJJ_RS01975 at 377.885 kb on + strand, within MMJJ_RS01975 at 377.887 kb on - strand, within MMJJ_RS01975 at 377.888 kb on + strand, within MMJJ_RS01975 at 377.912 kb on - strand, within MMJJ_RS01975 at 377.965 kb on + strand, within MMJJ_RS01975 at 378.005 kb on + strand, within MMJJ_RS01975 at 378.007 kb on + strand, within MMJJ_RS01975 at 378.055 kb on - strand, within MMJJ_RS01975 at 378.056 kb on + strand, within MMJJ_RS01975 at 378.058 kb on + strand, within MMJJ_RS01975 at 378.099 kb on + strand, within MMJJ_RS01975 at 378.104 kb on + strand, within MMJJ_RS01975 at 378.127 kb on - strand, within MMJJ_RS01975 at 378.144 kb on - strand, within MMJJ_RS01975 at 378.178 kb on - strand, within MMJJ_RS01975 at 378.179 kb on + strand, within MMJJ_RS01975 at 378.179 kb on + strand, within MMJJ_RS01975 at 378.200 kb on - strand, within MMJJ_RS01975 at 378.238 kb on - strand at 378.243 kb on - strand at 378.244 kb on + strand at 378.244 kb on + strand at 378.260 kb on - strand at 378.260 kb on - strand at 378.260 kb on - strand at 378.280 kb on - strand at 378.312 kb on - strand at 378.313 kb on + strand at 378.313 kb on + strand at 378.318 kb on - strand at 378.327 kb on - strand at 378.330 kb on - strand at 378.330 kb on - strand at 378.330 kb on - strand at 378.331 kb on + strand at 378.333 kb on - strand at 378.333 kb on - strand at 378.333 kb on - strand at 378.333 kb on - strand at 378.333 kb on - strand at 378.333 kb on - strand at 378.334 kb on + strand at 378.334 kb on + strand at 378.334 kb on + strand at 378.334 kb on + strand at 378.334 kb on + strand at 378.334 kb on + strand at 378.335 kb on - strand at 378.335 kb on - strand at 378.336 kb on + strand at 378.336 kb on + strand at 378.336 kb on + strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.345 kb on + strand at 378.350 kb on - strand at 378.350 kb on - strand at 378.350 kb on - strand at 378.351 kb on + strand at 378.371 kb on - strand at 378.377 kb on + strand at 378.377 kb on + strand at 378.383 kb on - strand at 378.383 kb on - strand at 378.398 kb on - strand at 378.399 kb on + strand at 378.403 kb on - strand at 378.403 kb on - strand at 378.414 kb on - strand at 378.414 kb on - strand at 378.415 kb on + strand at 378.436 kb on - strand at 378.436 kb on - strand at 378.436 kb on - strand at 378.437 kb on + strand at 378.491 kb on - strand at 378.514 kb on - strand at 378.514 kb on - strand at 378.514 kb on - strand at 378.514 kb on - strand at 378.514 kb on - strand at 378.515 kb on + strand at 378.515 kb on + strand at 378.539 kb on - strand, within MMJJ_RS01980 at 378.539 kb on - strand, within MMJJ_RS01980 at 378.542 kb on + strand at 378.542 kb on + strand, within MMJJ_RS01980 at 378.589 kb on - strand, within MMJJ_RS01980 at 378.590 kb on + strand, within MMJJ_RS01980 at 378.623 kb on - strand, within MMJJ_RS01980 at 378.635 kb on - strand, within MMJJ_RS01980 at 378.636 kb on + strand, within MMJJ_RS01980 at 378.636 kb on + strand, within MMJJ_RS01980 at 378.665 kb on + strand, within MMJJ_RS01980 at 378.665 kb on + strand, within MMJJ_RS01980 at 378.670 kb on - strand, within MMJJ_RS01980 at 378.706 kb on - strand at 378.717 kb on - strand, within MMJJ_RS01980 at 378.870 kb on + strand, within MMJJ_RS01980 at 378.872 kb on + strand, within MMJJ_RS01980 at 378.872 kb on + strand, within MMJJ_RS01980 at 378.895 kb on - strand, within MMJJ_RS01980 at 378.943 kb on - strand, within MMJJ_RS01980 at 378.960 kb on - strand, within MMJJ_RS01980 at 378.961 kb on + strand, within MMJJ_RS01980 at 378.962 kb on - strand, within MMJJ_RS01980 at 378.962 kb on - strand, within MMJJ_RS01980 at 378.963 kb on + strand, within MMJJ_RS01980 at 378.963 kb on + strand, within MMJJ_RS01980 at 378.980 kb on + strand, within MMJJ_RS01980 at 378.982 kb on - strand, within MMJJ_RS01980 at 378.982 kb on - strand, within MMJJ_RS01980 at 379.007 kb on + strand, within MMJJ_RS01980 at 379.010 kb on + strand, within MMJJ_RS01980 at 379.010 kb on + strand, within MMJJ_RS01980 at 379.010 kb on + strand, within MMJJ_RS01980 at 379.049 kb on - strand, within MMJJ_RS01980 at 379.049 kb on - strand, within MMJJ_RS01980 at 379.051 kb on - strand, within MMJJ_RS01980 at 379.060 kb on - strand, within MMJJ_RS01980 at 379.061 kb on + strand, within MMJJ_RS01980 at 379.064 kb on + strand, within MMJJ_RS01980 at 379.077 kb on + strand, within MMJJ_RS01980 at 379.091 kb on - strand, within MMJJ_RS01980 at 379.092 kb on + strand, within MMJJ_RS01980 at 379.122 kb on + strand, within MMJJ_RS01980 at 379.178 kb on + strand, within MMJJ_RS01980 at 379.282 kb on - strand, within MMJJ_RS01980 at 379.291 kb on - strand, within MMJJ_RS01980 at 379.291 kb on - strand, within MMJJ_RS01980 at 379.456 kb on - strand, within MMJJ_RS01980 at 379.493 kb on + strand at 379.501 kb on - strand at 379.525 kb on + strand at 379.525 kb on + strand at 379.525 kb on + strand at 379.568 kb on + strand at 379.590 kb on + strand at 379.607 kb on - strand at 379.607 kb on - strand at 379.608 kb on + strand at 379.641 kb on - strand at 379.686 kb on - strand, within MMJJ_RS01985 at 379.687 kb on + strand, within MMJJ_RS01985 at 379.687 kb on + strand, within MMJJ_RS01985 at 379.696 kb on - strand, within MMJJ_RS01985 at 379.697 kb on + strand at 379.697 kb on + strand, within MMJJ_RS01985 at 379.697 kb on + strand, within MMJJ_RS01985 at 379.719 kb on - strand, within MMJJ_RS01985 at 379.738 kb on - strand, within MMJJ_RS01985 at 379.739 kb on + strand, within MMJJ_RS01985 at 379.743 kb on + strand, within MMJJ_RS01985 at 379.743 kb on + strand, within MMJJ_RS01985 at 379.743 kb on + strand, within MMJJ_RS01985 at 379.797 kb on - strand, within MMJJ_RS01985 at 379.845 kb on - strand, within MMJJ_RS01985 at 379.855 kb on + strand, within MMJJ_RS01985 at 379.858 kb on + strand, within MMJJ_RS01985 at 379.863 kb on - strand, within MMJJ_RS01985 at 379.864 kb on + strand, within MMJJ_RS01985 at 379.864 kb on + strand, within MMJJ_RS01985 at 379.882 kb on - strand, within MMJJ_RS01985 at 379.882 kb on - strand, within MMJJ_RS01985 at 379.882 kb on - strand, within MMJJ_RS01985 at 379.883 kb on + strand, within MMJJ_RS01985 at 379.883 kb on + strand, within MMJJ_RS01985 at 379.902 kb on - strand, within MMJJ_RS01985 at 379.954 kb on + strand, within MMJJ_RS01985 at 379.983 kb on - strand, within MMJJ_RS01985 at 380.019 kb on - strand, within MMJJ_RS01985 at 380.060 kb on - strand, within MMJJ_RS01985 at 380.061 kb on + strand, within MMJJ_RS01985 at 380.061 kb on + strand, within MMJJ_RS01985 at 380.061 kb on + strand, within MMJJ_RS01985 at 380.068 kb on - strand, within MMJJ_RS01985 at 380.092 kb on - strand, within MMJJ_RS01985 at 380.092 kb on - strand, within MMJJ_RS01985 at 380.093 kb on + strand, within MMJJ_RS01985 at 380.093 kb on + strand, within MMJJ_RS01985 at 380.096 kb on - strand at 380.096 kb on - strand, within MMJJ_RS01985 at 380.096 kb on - strand, within MMJJ_RS01985 at 380.097 kb on + strand, within MMJJ_RS01985 at 380.097 kb on + strand, within MMJJ_RS01985 at 380.098 kb on - strand, within MMJJ_RS01985 at 380.098 kb on - strand, within MMJJ_RS01985 at 380.128 kb on + strand, within MMJJ_RS01985 at 380.199 kb on - strand, within MMJJ_RS01985 at 380.200 kb on + strand, within MMJJ_RS01985 at 380.200 kb on + strand, within MMJJ_RS01985 at 380.243 kb on - strand at 380.243 kb on - strand at 380.284 kb on + strand at 380.290 kb on + strand at 380.297 kb on - strand at 380.297 kb on - strand at 380.298 kb on + strand at 380.303 kb on + strand at 380.328 kb on - strand at 380.328 kb on - strand at 380.379 kb on - strand, within MMJJ_RS01990 at 380.409 kb on + strand, within MMJJ_RS01990 at 380.421 kb on - strand, within MMJJ_RS01990 at 380.436 kb on + strand, within MMJJ_RS01990 at 380.436 kb on + strand, within MMJJ_RS01990
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 377,415 - MMJJ_RS01970 0.50 -1.4 377,616 + MMJJ_RS01975 0.12 -5.0 377,645 - MMJJ_RS01975 0.16 +0.0 377,659 + MMJJ_RS01975 0.18 -3.7 377,661 - MMJJ_RS01975 0.18 -1.6 377,662 + MMJJ_RS01975 0.18 -2.7 377,662 + MMJJ_RS01975 0.18 -2.4 377,677 - MMJJ_RS01975 0.20 -2.6 377,794 + MMJJ_RS01975 0.36 -1.9 377,798 + MMJJ_RS01975 0.36 -2.7 377,841 - MMJJ_RS01975 0.42 -0.6 377,884 - MMJJ_RS01975 0.47 -3.0 377,885 + MMJJ_RS01975 0.47 -1.2 377,885 + MMJJ_RS01975 0.47 -2.6 377,887 - MMJJ_RS01975 0.48 -2.2 377,888 + MMJJ_RS01975 0.48 -3.9 377,912 - MMJJ_RS01975 0.51 -3.9 377,965 + MMJJ_RS01975 0.58 -4.9 378,005 + MMJJ_RS01975 0.63 -1.7 378,007 + MMJJ_RS01975 0.63 -1.7 378,055 - MMJJ_RS01975 0.70 -5.0 378,056 + MMJJ_RS01975 0.70 -3.0 378,058 + MMJJ_RS01975 0.70 -3.2 378,099 + MMJJ_RS01975 0.75 -5.2 378,104 + MMJJ_RS01975 0.76 -3.9 378,127 - MMJJ_RS01975 0.79 -4.5 378,144 - MMJJ_RS01975 0.81 -2.7 378,178 - MMJJ_RS01975 0.86 -2.5 378,179 + MMJJ_RS01975 0.86 -4.2 378,179 + MMJJ_RS01975 0.86 -4.5 378,200 - MMJJ_RS01975 0.88 -3.5 378,238 - -4.3 378,243 - -2.8 378,244 + -3.2 378,244 + -2.7 378,260 - -1.1 378,260 - -2.5 378,260 - -4.6 378,280 - -2.3 378,312 - -3.6 378,313 + -3.7 378,313 + -3.6 378,318 - -3.0 378,327 - -5.0 378,330 - -3.6 378,330 - +1.4 378,330 - -1.5 378,331 + -3.2 378,333 - -3.0 378,333 - -1.1 378,333 - -1.3 378,333 - -1.7 378,333 - -2.2 378,333 - -1.2 378,334 + +0.4 378,334 + -3.4 378,334 + -1.6 378,334 + -4.1 378,334 + -2.2 378,334 + -1.9 378,335 - +2.4 378,335 - +0.9 378,336 + -1.9 378,336 + -1.7 378,336 + -2.5 378,337 - +3.8 378,337 - +1.1 378,337 - +1.1 378,337 - +3.1 378,337 - +1.7 378,337 - +1.6 378,337 - +3.3 378,337 - +2.3 378,338 + +2.0 378,338 + +2.0 378,338 + +1.1 378,338 + +1.8 378,338 + +1.2 378,338 + +3.5 378,338 + +1.2 378,338 + +3.1 378,338 + +3.0 378,338 + +2.4 378,338 + +1.7 378,338 + +2.7 378,338 + +1.3 378,338 + +3.2 378,345 + -0.4 378,350 - +3.0 378,350 - +2.2 378,350 - -0.1 378,351 + -0.4 378,371 - +2.9 378,377 + +2.4 378,377 + +1.9 378,383 - +3.9 378,383 - +1.7 378,398 - +2.8 378,399 + +2.9 378,403 - +0.7 378,403 - +1.3 378,414 - +2.9 378,414 - +2.1 378,415 + +4.9 378,436 - +2.0 378,436 - +0.7 378,436 - +1.7 378,437 + +2.8 378,491 - +2.4 378,514 - +2.2 378,514 - -1.3 378,514 - +3.4 378,514 - +2.6 378,514 - +2.4 378,515 + +2.4 378,515 + +2.2 378,539 - MMJJ_RS01980 0.11 +2.9 378,539 - MMJJ_RS01980 0.11 +3.6 378,542 + +1.8 378,542 + MMJJ_RS01980 0.12 -1.4 378,589 - MMJJ_RS01980 0.16 +1.7 378,590 + MMJJ_RS01980 0.16 -0.6 378,623 - MMJJ_RS01980 0.19 +2.0 378,635 - MMJJ_RS01980 0.20 +2.6 378,636 + MMJJ_RS01980 0.20 -0.5 378,636 + MMJJ_RS01980 0.20 +1.1 378,665 + MMJJ_RS01980 0.22 +3.6 378,665 + MMJJ_RS01980 0.22 +3.2 378,670 - MMJJ_RS01980 0.23 -0.9 378,706 - +3.4 378,717 - MMJJ_RS01980 0.27 +2.5 378,870 + MMJJ_RS01980 0.40 +3.0 378,872 + MMJJ_RS01980 0.40 +2.0 378,872 + MMJJ_RS01980 0.40 +2.7 378,895 - MMJJ_RS01980 0.42 +1.2 378,943 - MMJJ_RS01980 0.46 +3.1 378,960 - MMJJ_RS01980 0.47 +3.5 378,961 + MMJJ_RS01980 0.48 +3.2 378,962 - MMJJ_RS01980 0.48 +2.6 378,962 - MMJJ_RS01980 0.48 +1.7 378,963 + MMJJ_RS01980 0.48 +0.8 378,963 + MMJJ_RS01980 0.48 +1.6 378,980 + MMJJ_RS01980 0.49 +1.4 378,982 - MMJJ_RS01980 0.49 +3.0 378,982 - MMJJ_RS01980 0.49 +2.5 379,007 + MMJJ_RS01980 0.51 +2.5 379,010 + MMJJ_RS01980 0.52 +1.8 379,010 + MMJJ_RS01980 0.52 +2.8 379,010 + MMJJ_RS01980 0.52 +2.2 379,049 - MMJJ_RS01980 0.55 +1.5 379,049 - MMJJ_RS01980 0.55 +1.3 379,051 - MMJJ_RS01980 0.55 +2.4 379,060 - MMJJ_RS01980 0.56 +3.5 379,061 + MMJJ_RS01980 0.56 +0.5 379,064 + MMJJ_RS01980 0.56 +2.9 379,077 + MMJJ_RS01980 0.57 +2.7 379,091 - MMJJ_RS01980 0.59 +2.4 379,092 + MMJJ_RS01980 0.59 +3.1 379,122 + MMJJ_RS01980 0.61 -1.2 379,178 + MMJJ_RS01980 0.66 +2.4 379,282 - MMJJ_RS01980 0.75 +0.5 379,291 - MMJJ_RS01980 0.76 +1.3 379,291 - MMJJ_RS01980 0.76 +3.1 379,456 - MMJJ_RS01980 0.90 +1.5 379,493 + -1.6 379,501 - +1.8 379,525 + +0.9 379,525 + +0.5 379,525 + +1.0 379,568 + +0.1 379,590 + +1.3 379,607 - +0.9 379,607 - -0.7 379,608 + -3.3 379,641 - +0.2 379,686 - MMJJ_RS01985 0.12 +0.2 379,687 + MMJJ_RS01985 0.12 -0.7 379,687 + MMJJ_RS01985 0.12 +0.2 379,696 - MMJJ_RS01985 0.14 +0.9 379,697 + +0.4 379,697 + MMJJ_RS01985 0.14 -3.6 379,697 + MMJJ_RS01985 0.14 +0.1 379,719 - MMJJ_RS01985 0.17 -1.7 379,738 - MMJJ_RS01985 0.20 -3.1 379,739 + MMJJ_RS01985 0.20 +0.4 379,743 + MMJJ_RS01985 0.20 +2.5 379,743 + MMJJ_RS01985 0.20 +0.7 379,743 + MMJJ_RS01985 0.20 +1.3 379,797 - MMJJ_RS01985 0.28 +2.2 379,845 - MMJJ_RS01985 0.35 -0.2 379,855 + MMJJ_RS01985 0.36 +1.4 379,858 + MMJJ_RS01985 0.37 +0.5 379,863 - MMJJ_RS01985 0.37 -2.9 379,864 + MMJJ_RS01985 0.38 +0.5 379,864 + MMJJ_RS01985 0.38 +0.4 379,882 - MMJJ_RS01985 0.40 +0.1 379,882 - MMJJ_RS01985 0.40 -0.3 379,882 - MMJJ_RS01985 0.40 -2.3 379,883 + MMJJ_RS01985 0.40 -0.1 379,883 + MMJJ_RS01985 0.40 +0.8 379,902 - MMJJ_RS01985 0.43 +1.0 379,954 + MMJJ_RS01985 0.51 +1.4 379,983 - MMJJ_RS01985 0.55 -0.5 380,019 - MMJJ_RS01985 0.60 +0.5 380,060 - MMJJ_RS01985 0.66 +0.6 380,061 + MMJJ_RS01985 0.66 -1.9 380,061 + MMJJ_RS01985 0.66 +0.9 380,061 + MMJJ_RS01985 0.66 -0.0 380,068 - MMJJ_RS01985 0.67 -2.6 380,092 - MMJJ_RS01985 0.70 -0.1 380,092 - MMJJ_RS01985 0.70 -0.3 380,093 + MMJJ_RS01985 0.70 -1.2 380,093 + MMJJ_RS01985 0.70 +1.3 380,096 - -0.2 380,096 - MMJJ_RS01985 0.71 +0.6 380,096 - MMJJ_RS01985 0.71 +0.5 380,097 + MMJJ_RS01985 0.71 -2.2 380,097 + MMJJ_RS01985 0.71 +2.0 380,098 - MMJJ_RS01985 0.71 +0.5 380,098 - MMJJ_RS01985 0.71 +1.4 380,128 + MMJJ_RS01985 0.75 +0.2 380,199 - MMJJ_RS01985 0.86 -1.7 380,200 + MMJJ_RS01985 0.86 -0.3 380,200 + MMJJ_RS01985 0.86 -1.5 380,243 - -3.0 380,243 - +0.3 380,284 + -0.0 380,290 + +0.2 380,297 - +2.2 380,297 - +1.1 380,298 + +0.6 380,303 + -1.4 380,328 - -0.3 380,328 - +0.4 380,379 - MMJJ_RS01990 0.17 -0.8 380,409 + MMJJ_RS01990 0.24 -0.3 380,421 - MMJJ_RS01990 0.27 -1.4 380,436 + MMJJ_RS01990 0.30 +0.2 380,436 + MMJJ_RS01990 0.30 -1.7
Or see this region's nucleotide sequence