Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01965 and MMJJ_RS01970 are separated by 261 nucleotides MMJJ_RS01970 and MMJJ_RS01975 are separated by 5 nucleotides MMJJ_RS01975 and MMJJ_RS01980 are separated by 116 nucleotides
MMJJ_RS01965: MMJJ_RS01965 - radical SAM protein, at 375,521 to 377,053
_RS01965
MMJJ_RS01970: MMJJ_RS01970 - hypothetical protein, at 377,315 to 377,515
_RS01970
MMJJ_RS01975: MMJJ_RS01975 - diphthine synthase, at 377,521 to 378,288
_RS01975
MMJJ_RS01980: MMJJ_RS01980 - cation:proton antiporter, at 378,405 to 379,574
_RS01980
Position (kb) 377
378
379 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 376.582 kb on - strand, within MMJJ_RS01965 at 376.703 kb on - strand, within MMJJ_RS01965 at 376.703 kb on - strand, within MMJJ_RS01965 at 376.779 kb on - strand, within MMJJ_RS01965 at 376.865 kb on - strand, within MMJJ_RS01965 at 376.872 kb on - strand, within MMJJ_RS01965 at 376.873 kb on + strand, within MMJJ_RS01965 at 376.873 kb on + strand, within MMJJ_RS01965 at 376.880 kb on + strand, within MMJJ_RS01965 at 376.930 kb on + strand at 376.930 kb on + strand at 376.990 kb on - strand at 376.991 kb on + strand at 376.993 kb on + strand at 377.026 kb on - strand at 377.027 kb on + strand at 377.043 kb on + strand at 377.068 kb on - strand at 377.086 kb on - strand at 377.086 kb on - strand at 377.087 kb on + strand at 377.087 kb on + strand at 377.087 kb on + strand at 377.087 kb on + strand at 377.088 kb on - strand at 377.088 kb on - strand at 377.088 kb on - strand at 377.088 kb on - strand at 377.088 kb on - strand at 377.088 kb on - strand at 377.088 kb on - strand at 377.089 kb on + strand at 377.089 kb on + strand at 377.089 kb on + strand at 377.089 kb on + strand at 377.089 kb on + strand at 377.095 kb on - strand at 377.095 kb on - strand at 377.095 kb on - strand at 377.095 kb on - strand at 377.096 kb on + strand at 377.106 kb on - strand at 377.107 kb on + strand at 377.110 kb on - strand at 377.110 kb on - strand at 377.111 kb on + strand at 377.111 kb on + strand at 377.111 kb on + strand at 377.112 kb on - strand at 377.112 kb on - strand at 377.113 kb on + strand at 377.113 kb on + strand at 377.113 kb on + strand at 377.144 kb on - strand at 377.193 kb on + strand at 377.205 kb on - strand at 377.225 kb on - strand at 377.225 kb on - strand at 377.225 kb on - strand at 377.227 kb on - strand at 377.245 kb on - strand at 377.245 kb on - strand at 377.247 kb on - strand at 377.247 kb on - strand at 377.273 kb on - strand at 377.274 kb on + strand at 377.279 kb on - strand at 377.294 kb on - strand at 377.298 kb on - strand at 377.298 kb on - strand at 377.298 kb on - strand at 377.299 kb on + strand at 377.299 kb on + strand at 377.299 kb on + strand at 377.299 kb on + strand at 377.299 kb on + strand at 377.301 kb on + strand at 377.301 kb on + strand at 377.337 kb on - strand, within MMJJ_RS01970 at 377.339 kb on - strand, within MMJJ_RS01970 at 377.339 kb on - strand, within MMJJ_RS01970 at 377.339 kb on - strand, within MMJJ_RS01970 at 377.339 kb on - strand, within MMJJ_RS01970 at 377.339 kb on - strand, within MMJJ_RS01970 at 377.339 kb on - strand, within MMJJ_RS01970 at 377.340 kb on + strand, within MMJJ_RS01970 at 377.340 kb on + strand, within MMJJ_RS01970 at 377.390 kb on - strand, within MMJJ_RS01970 at 377.415 kb on - strand, within MMJJ_RS01970 at 377.616 kb on + strand, within MMJJ_RS01975 at 377.645 kb on - strand, within MMJJ_RS01975 at 377.659 kb on + strand, within MMJJ_RS01975 at 377.661 kb on - strand, within MMJJ_RS01975 at 377.662 kb on + strand, within MMJJ_RS01975 at 377.662 kb on + strand, within MMJJ_RS01975 at 377.677 kb on - strand, within MMJJ_RS01975 at 377.794 kb on + strand, within MMJJ_RS01975 at 377.798 kb on + strand, within MMJJ_RS01975 at 377.841 kb on - strand, within MMJJ_RS01975 at 377.884 kb on - strand, within MMJJ_RS01975 at 377.885 kb on + strand, within MMJJ_RS01975 at 377.885 kb on + strand, within MMJJ_RS01975 at 377.887 kb on - strand, within MMJJ_RS01975 at 377.888 kb on + strand, within MMJJ_RS01975 at 377.912 kb on - strand, within MMJJ_RS01975 at 377.965 kb on + strand, within MMJJ_RS01975 at 378.005 kb on + strand, within MMJJ_RS01975 at 378.007 kb on + strand, within MMJJ_RS01975 at 378.055 kb on - strand, within MMJJ_RS01975 at 378.056 kb on + strand, within MMJJ_RS01975 at 378.058 kb on + strand, within MMJJ_RS01975 at 378.099 kb on + strand, within MMJJ_RS01975 at 378.104 kb on + strand, within MMJJ_RS01975 at 378.127 kb on - strand, within MMJJ_RS01975 at 378.144 kb on - strand, within MMJJ_RS01975 at 378.178 kb on - strand, within MMJJ_RS01975 at 378.179 kb on + strand, within MMJJ_RS01975 at 378.179 kb on + strand, within MMJJ_RS01975 at 378.200 kb on - strand, within MMJJ_RS01975 at 378.238 kb on - strand at 378.243 kb on - strand at 378.244 kb on + strand at 378.244 kb on + strand at 378.260 kb on - strand at 378.260 kb on - strand at 378.260 kb on - strand at 378.280 kb on - strand at 378.312 kb on - strand at 378.313 kb on + strand at 378.313 kb on + strand at 378.318 kb on - strand at 378.327 kb on - strand at 378.330 kb on - strand at 378.330 kb on - strand at 378.330 kb on - strand at 378.331 kb on + strand at 378.333 kb on - strand at 378.333 kb on - strand at 378.333 kb on - strand at 378.333 kb on - strand at 378.333 kb on - strand at 378.333 kb on - strand at 378.334 kb on + strand at 378.334 kb on + strand at 378.334 kb on + strand at 378.334 kb on + strand at 378.334 kb on + strand at 378.334 kb on + strand at 378.335 kb on - strand at 378.335 kb on - strand at 378.336 kb on + strand at 378.336 kb on + strand at 378.336 kb on + strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.337 kb on - strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.338 kb on + strand at 378.345 kb on + strand at 378.350 kb on - strand at 378.350 kb on - strand at 378.350 kb on - strand at 378.351 kb on + strand at 378.371 kb on - strand at 378.377 kb on + strand at 378.377 kb on + strand at 378.383 kb on - strand at 378.383 kb on - strand at 378.398 kb on - strand at 378.399 kb on + strand at 378.403 kb on - strand at 378.403 kb on - strand at 378.414 kb on - strand at 378.414 kb on - strand at 378.415 kb on + strand at 378.436 kb on - strand at 378.436 kb on - strand at 378.436 kb on - strand at 378.437 kb on + strand at 378.491 kb on - strand at 378.514 kb on - strand at 378.514 kb on - strand at 378.514 kb on - strand at 378.514 kb on - strand at 378.514 kb on - strand at 378.515 kb on + strand at 378.515 kb on + strand at 378.539 kb on - strand, within MMJJ_RS01980 at 378.539 kb on - strand, within MMJJ_RS01980 at 378.542 kb on + strand at 378.542 kb on + strand, within MMJJ_RS01980 at 378.589 kb on - strand, within MMJJ_RS01980 at 378.590 kb on + strand, within MMJJ_RS01980 at 378.623 kb on - strand, within MMJJ_RS01980 at 378.635 kb on - strand, within MMJJ_RS01980 at 378.636 kb on + strand, within MMJJ_RS01980 at 378.636 kb on + strand, within MMJJ_RS01980 at 378.665 kb on + strand, within MMJJ_RS01980 at 378.665 kb on + strand, within MMJJ_RS01980 at 378.670 kb on - strand, within MMJJ_RS01980 at 378.706 kb on - strand at 378.717 kb on - strand, within MMJJ_RS01980 at 378.870 kb on + strand, within MMJJ_RS01980 at 378.872 kb on + strand, within MMJJ_RS01980 at 378.872 kb on + strand, within MMJJ_RS01980 at 378.895 kb on - strand, within MMJJ_RS01980 at 378.943 kb on - strand, within MMJJ_RS01980 at 378.960 kb on - strand, within MMJJ_RS01980 at 378.961 kb on + strand, within MMJJ_RS01980 at 378.962 kb on - strand, within MMJJ_RS01980 at 378.962 kb on - strand, within MMJJ_RS01980 at 378.963 kb on + strand, within MMJJ_RS01980 at 378.963 kb on + strand, within MMJJ_RS01980 at 378.980 kb on + strand, within MMJJ_RS01980 at 378.982 kb on - strand, within MMJJ_RS01980 at 378.982 kb on - strand, within MMJJ_RS01980 at 379.007 kb on + strand, within MMJJ_RS01980 at 379.010 kb on + strand, within MMJJ_RS01980 at 379.010 kb on + strand, within MMJJ_RS01980 at 379.010 kb on + strand, within MMJJ_RS01980 at 379.049 kb on - strand, within MMJJ_RS01980 at 379.049 kb on - strand, within MMJJ_RS01980 at 379.051 kb on - strand, within MMJJ_RS01980 at 379.060 kb on - strand, within MMJJ_RS01980 at 379.061 kb on + strand, within MMJJ_RS01980 at 379.064 kb on + strand, within MMJJ_RS01980 at 379.077 kb on + strand, within MMJJ_RS01980 at 379.091 kb on - strand, within MMJJ_RS01980 at 379.092 kb on + strand, within MMJJ_RS01980 at 379.122 kb on + strand, within MMJJ_RS01980 at 379.178 kb on + strand, within MMJJ_RS01980 at 379.282 kb on - strand, within MMJJ_RS01980
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 376,582 - MMJJ_RS01965 0.69 -1.8 376,703 - MMJJ_RS01965 0.77 -1.3 376,703 - MMJJ_RS01965 0.77 +1.0 376,779 - MMJJ_RS01965 0.82 +0.1 376,865 - MMJJ_RS01965 0.88 +0.9 376,872 - MMJJ_RS01965 0.88 -0.6 376,873 + MMJJ_RS01965 0.88 -2.8 376,873 + MMJJ_RS01965 0.88 -1.0 376,880 + MMJJ_RS01965 0.89 +2.9 376,930 + -1.5 376,930 + -1.4 376,990 - +1.6 376,991 + +0.6 376,993 + +1.4 377,026 - -0.3 377,027 + +1.1 377,043 + +1.4 377,068 - -1.4 377,086 - +2.1 377,086 - -1.4 377,087 + +0.7 377,087 + -0.7 377,087 + -3.3 377,087 + -1.7 377,088 - +0.5 377,088 - -1.6 377,088 - -2.2 377,088 - +0.1 377,088 - +0.4 377,088 - +0.0 377,088 - +0.4 377,089 + -1.6 377,089 + -0.0 377,089 + +0.7 377,089 + +1.0 377,089 + -1.2 377,095 - -2.4 377,095 - +1.1 377,095 - -0.7 377,095 - -3.3 377,096 + +0.4 377,106 - -0.2 377,107 + +0.7 377,110 - -2.2 377,110 - -1.2 377,111 + -0.2 377,111 + +1.0 377,111 + +1.0 377,112 - +0.6 377,112 - -0.0 377,113 + -3.3 377,113 + -3.5 377,113 + +0.9 377,144 - +2.1 377,193 + +1.5 377,205 - -0.3 377,225 - +0.1 377,225 - -0.6 377,225 - -0.4 377,227 - +0.4 377,245 - -3.7 377,245 - +0.0 377,247 - -0.3 377,247 - +0.2 377,273 - +0.4 377,274 + -2.7 377,279 - -1.6 377,294 - -0.9 377,298 - +2.3 377,298 - -1.9 377,298 - +2.3 377,299 + -0.0 377,299 + +0.5 377,299 + +0.4 377,299 + +0.4 377,299 + +2.0 377,301 + +1.5 377,301 + -1.9 377,337 - MMJJ_RS01970 0.11 +0.3 377,339 - MMJJ_RS01970 0.12 -0.0 377,339 - MMJJ_RS01970 0.12 +0.4 377,339 - MMJJ_RS01970 0.12 +1.6 377,339 - MMJJ_RS01970 0.12 -0.6 377,339 - MMJJ_RS01970 0.12 -0.4 377,339 - MMJJ_RS01970 0.12 +0.3 377,340 + MMJJ_RS01970 0.12 -0.4 377,340 + MMJJ_RS01970 0.12 +1.5 377,390 - MMJJ_RS01970 0.37 -1.1 377,415 - MMJJ_RS01970 0.50 -1.4 377,616 + MMJJ_RS01975 0.12 -5.0 377,645 - MMJJ_RS01975 0.16 +0.0 377,659 + MMJJ_RS01975 0.18 -3.7 377,661 - MMJJ_RS01975 0.18 -1.6 377,662 + MMJJ_RS01975 0.18 -2.7 377,662 + MMJJ_RS01975 0.18 -2.4 377,677 - MMJJ_RS01975 0.20 -2.6 377,794 + MMJJ_RS01975 0.36 -1.9 377,798 + MMJJ_RS01975 0.36 -2.7 377,841 - MMJJ_RS01975 0.42 -0.6 377,884 - MMJJ_RS01975 0.47 -3.0 377,885 + MMJJ_RS01975 0.47 -1.2 377,885 + MMJJ_RS01975 0.47 -2.6 377,887 - MMJJ_RS01975 0.48 -2.2 377,888 + MMJJ_RS01975 0.48 -3.9 377,912 - MMJJ_RS01975 0.51 -3.9 377,965 + MMJJ_RS01975 0.58 -4.9 378,005 + MMJJ_RS01975 0.63 -1.7 378,007 + MMJJ_RS01975 0.63 -1.7 378,055 - MMJJ_RS01975 0.70 -5.0 378,056 + MMJJ_RS01975 0.70 -3.0 378,058 + MMJJ_RS01975 0.70 -3.2 378,099 + MMJJ_RS01975 0.75 -5.2 378,104 + MMJJ_RS01975 0.76 -3.9 378,127 - MMJJ_RS01975 0.79 -4.5 378,144 - MMJJ_RS01975 0.81 -2.7 378,178 - MMJJ_RS01975 0.86 -2.5 378,179 + MMJJ_RS01975 0.86 -4.2 378,179 + MMJJ_RS01975 0.86 -4.5 378,200 - MMJJ_RS01975 0.88 -3.5 378,238 - -4.3 378,243 - -2.8 378,244 + -3.2 378,244 + -2.7 378,260 - -1.1 378,260 - -2.5 378,260 - -4.6 378,280 - -2.3 378,312 - -3.6 378,313 + -3.7 378,313 + -3.6 378,318 - -3.0 378,327 - -5.0 378,330 - -3.6 378,330 - +1.4 378,330 - -1.5 378,331 + -3.2 378,333 - -3.0 378,333 - -1.1 378,333 - -1.3 378,333 - -1.7 378,333 - -2.2 378,333 - -1.2 378,334 + +0.4 378,334 + -3.4 378,334 + -1.6 378,334 + -4.1 378,334 + -2.2 378,334 + -1.9 378,335 - +2.4 378,335 - +0.9 378,336 + -1.9 378,336 + -1.7 378,336 + -2.5 378,337 - +3.8 378,337 - +1.1 378,337 - +1.1 378,337 - +3.1 378,337 - +1.7 378,337 - +1.6 378,337 - +3.3 378,337 - +2.3 378,338 + +2.0 378,338 + +2.0 378,338 + +1.1 378,338 + +1.8 378,338 + +1.2 378,338 + +3.5 378,338 + +1.2 378,338 + +3.1 378,338 + +3.0 378,338 + +2.4 378,338 + +1.7 378,338 + +2.7 378,338 + +1.3 378,338 + +3.2 378,345 + -0.4 378,350 - +3.0 378,350 - +2.2 378,350 - -0.1 378,351 + -0.4 378,371 - +2.9 378,377 + +2.4 378,377 + +1.9 378,383 - +3.9 378,383 - +1.7 378,398 - +2.8 378,399 + +2.9 378,403 - +0.7 378,403 - +1.3 378,414 - +2.9 378,414 - +2.1 378,415 + +4.9 378,436 - +2.0 378,436 - +0.7 378,436 - +1.7 378,437 + +2.8 378,491 - +2.4 378,514 - +2.2 378,514 - -1.3 378,514 - +3.4 378,514 - +2.6 378,514 - +2.4 378,515 + +2.4 378,515 + +2.2 378,539 - MMJJ_RS01980 0.11 +2.9 378,539 - MMJJ_RS01980 0.11 +3.6 378,542 + +1.8 378,542 + MMJJ_RS01980 0.12 -1.4 378,589 - MMJJ_RS01980 0.16 +1.7 378,590 + MMJJ_RS01980 0.16 -0.6 378,623 - MMJJ_RS01980 0.19 +2.0 378,635 - MMJJ_RS01980 0.20 +2.6 378,636 + MMJJ_RS01980 0.20 -0.5 378,636 + MMJJ_RS01980 0.20 +1.1 378,665 + MMJJ_RS01980 0.22 +3.6 378,665 + MMJJ_RS01980 0.22 +3.2 378,670 - MMJJ_RS01980 0.23 -0.9 378,706 - +3.4 378,717 - MMJJ_RS01980 0.27 +2.5 378,870 + MMJJ_RS01980 0.40 +3.0 378,872 + MMJJ_RS01980 0.40 +2.0 378,872 + MMJJ_RS01980 0.40 +2.7 378,895 - MMJJ_RS01980 0.42 +1.2 378,943 - MMJJ_RS01980 0.46 +3.1 378,960 - MMJJ_RS01980 0.47 +3.5 378,961 + MMJJ_RS01980 0.48 +3.2 378,962 - MMJJ_RS01980 0.48 +2.6 378,962 - MMJJ_RS01980 0.48 +1.7 378,963 + MMJJ_RS01980 0.48 +0.8 378,963 + MMJJ_RS01980 0.48 +1.6 378,980 + MMJJ_RS01980 0.49 +1.4 378,982 - MMJJ_RS01980 0.49 +3.0 378,982 - MMJJ_RS01980 0.49 +2.5 379,007 + MMJJ_RS01980 0.51 +2.5 379,010 + MMJJ_RS01980 0.52 +1.8 379,010 + MMJJ_RS01980 0.52 +2.8 379,010 + MMJJ_RS01980 0.52 +2.2 379,049 - MMJJ_RS01980 0.55 +1.5 379,049 - MMJJ_RS01980 0.55 +1.3 379,051 - MMJJ_RS01980 0.55 +2.4 379,060 - MMJJ_RS01980 0.56 +3.5 379,061 + MMJJ_RS01980 0.56 +0.5 379,064 + MMJJ_RS01980 0.56 +2.9 379,077 + MMJJ_RS01980 0.57 +2.7 379,091 - MMJJ_RS01980 0.59 +2.4 379,092 + MMJJ_RS01980 0.59 +3.1 379,122 + MMJJ_RS01980 0.61 -1.2 379,178 + MMJJ_RS01980 0.66 +2.4 379,282 - MMJJ_RS01980 0.75 +0.5
Or see this region's nucleotide sequence