Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01675 and MMJJ_RS01680 are separated by 16 nucleotides MMJJ_RS01680 and MMJJ_RS01685 are separated by 4 nucleotides
MMJJ_RS01675: MMJJ_RS01675 - malate dehydrogenase, at 324,664 to 325,608
_RS01675
MMJJ_RS01680: MMJJ_RS01680 - cation diffusion facilitator family transporter, at 325,625 to 326,500
_RS01680
MMJJ_RS01685: MMJJ_RS01685 - DUF354 domain-containing protein, at 326,505 to 327,518
_RS01685
Position (kb)
325
326
327 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 324.633 kb on + strand at 324.633 kb on + strand at 324.633 kb on + strand at 324.645 kb on - strand at 324.646 kb on + strand at 324.736 kb on - strand at 324.780 kb on - strand, within MMJJ_RS01675 at 324.808 kb on - strand, within MMJJ_RS01675 at 324.837 kb on - strand, within MMJJ_RS01675 at 324.840 kb on - strand, within MMJJ_RS01675 at 324.841 kb on + strand, within MMJJ_RS01675 at 324.841 kb on + strand, within MMJJ_RS01675 at 324.851 kb on - strand, within MMJJ_RS01675 at 324.851 kb on - strand, within MMJJ_RS01675 at 324.851 kb on - strand, within MMJJ_RS01675 at 324.852 kb on + strand, within MMJJ_RS01675 at 324.852 kb on + strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.870 kb on + strand, within MMJJ_RS01675 at 324.876 kb on - strand, within MMJJ_RS01675 at 324.876 kb on - strand, within MMJJ_RS01675 at 324.876 kb on - strand, within MMJJ_RS01675 at 324.876 kb on - strand, within MMJJ_RS01675 at 324.877 kb on + strand, within MMJJ_RS01675 at 324.877 kb on + strand, within MMJJ_RS01675 at 324.877 kb on + strand, within MMJJ_RS01675 at 324.882 kb on - strand, within MMJJ_RS01675 at 324.891 kb on - strand, within MMJJ_RS01675 at 324.891 kb on - strand, within MMJJ_RS01675 at 324.892 kb on + strand, within MMJJ_RS01675 at 324.892 kb on + strand, within MMJJ_RS01675 at 324.899 kb on - strand, within MMJJ_RS01675 at 324.899 kb on - strand, within MMJJ_RS01675 at 324.904 kb on + strand, within MMJJ_RS01675 at 324.904 kb on + strand, within MMJJ_RS01675 at 324.948 kb on - strand, within MMJJ_RS01675 at 324.990 kb on - strand, within MMJJ_RS01675 at 324.990 kb on - strand, within MMJJ_RS01675 at 324.991 kb on + strand at 324.991 kb on + strand, within MMJJ_RS01675 at 324.996 kb on - strand at 324.996 kb on - strand, within MMJJ_RS01675 at 324.997 kb on + strand, within MMJJ_RS01675 at 324.997 kb on + strand, within MMJJ_RS01675 at 325.011 kb on - strand, within MMJJ_RS01675 at 325.011 kb on - strand, within MMJJ_RS01675 at 325.011 kb on - strand, within MMJJ_RS01675 at 325.012 kb on + strand, within MMJJ_RS01675 at 325.017 kb on + strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.052 kb on + strand, within MMJJ_RS01675 at 325.052 kb on + strand, within MMJJ_RS01675 at 325.052 kb on + strand, within MMJJ_RS01675 at 325.075 kb on - strand, within MMJJ_RS01675 at 325.128 kb on - strand, within MMJJ_RS01675 at 325.134 kb on + strand, within MMJJ_RS01675 at 325.134 kb on + strand, within MMJJ_RS01675 at 325.174 kb on + strand, within MMJJ_RS01675 at 325.174 kb on + strand, within MMJJ_RS01675 at 325.235 kb on - strand, within MMJJ_RS01675 at 325.236 kb on + strand, within MMJJ_RS01675 at 325.236 kb on + strand, within MMJJ_RS01675 at 325.267 kb on - strand, within MMJJ_RS01675 at 325.305 kb on - strand, within MMJJ_RS01675 at 325.306 kb on + strand, within MMJJ_RS01675 at 325.312 kb on - strand, within MMJJ_RS01675 at 325.329 kb on + strand, within MMJJ_RS01675 at 325.329 kb on + strand, within MMJJ_RS01675 at 325.373 kb on - strand, within MMJJ_RS01675 at 325.382 kb on - strand, within MMJJ_RS01675 at 325.382 kb on - strand, within MMJJ_RS01675 at 325.383 kb on + strand, within MMJJ_RS01675 at 325.383 kb on + strand, within MMJJ_RS01675 at 325.383 kb on + strand, within MMJJ_RS01675 at 325.383 kb on + strand at 325.383 kb on + strand, within MMJJ_RS01675 at 325.383 kb on + strand, within MMJJ_RS01675 at 325.389 kb on - strand, within MMJJ_RS01675 at 325.389 kb on - strand, within MMJJ_RS01675 at 325.390 kb on + strand, within MMJJ_RS01675 at 325.472 kb on - strand, within MMJJ_RS01675 at 325.484 kb on - strand, within MMJJ_RS01675 at 325.515 kb on + strand at 325.571 kb on - strand at 325.571 kb on - strand at 325.572 kb on + strand at 325.602 kb on - strand at 325.603 kb on + strand at 325.650 kb on - strand at 325.650 kb on - strand at 325.650 kb on - strand at 325.657 kb on - strand at 325.715 kb on + strand, within MMJJ_RS01680 at 325.722 kb on - strand, within MMJJ_RS01680 at 325.722 kb on - strand, within MMJJ_RS01680 at 325.736 kb on + strand, within MMJJ_RS01680 at 325.743 kb on - strand, within MMJJ_RS01680 at 325.743 kb on - strand, within MMJJ_RS01680 at 325.783 kb on - strand, within MMJJ_RS01680 at 325.980 kb on - strand, within MMJJ_RS01680 at 326.022 kb on + strand, within MMJJ_RS01680 at 326.028 kb on + strand, within MMJJ_RS01680 at 326.028 kb on + strand, within MMJJ_RS01680 at 326.121 kb on - strand, within MMJJ_RS01680 at 326.179 kb on + strand, within MMJJ_RS01680 at 326.190 kb on - strand, within MMJJ_RS01680 at 326.191 kb on + strand, within MMJJ_RS01680 at 326.195 kb on + strand, within MMJJ_RS01680 at 326.220 kb on + strand, within MMJJ_RS01680 at 326.270 kb on + strand, within MMJJ_RS01680 at 326.290 kb on + strand, within MMJJ_RS01680 at 326.319 kb on - strand, within MMJJ_RS01680 at 326.319 kb on - strand, within MMJJ_RS01680 at 326.320 kb on + strand, within MMJJ_RS01680 at 326.326 kb on + strand, within MMJJ_RS01680 at 326.357 kb on - strand, within MMJJ_RS01680 at 326.357 kb on - strand, within MMJJ_RS01680 at 326.410 kb on - strand, within MMJJ_RS01680 at 326.501 kb on - strand at 326.502 kb on + strand at 326.512 kb on + strand at 326.585 kb on - strand at 326.778 kb on - strand, within MMJJ_RS01685 at 327.161 kb on - strand, within MMJJ_RS01685 at 327.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 324,633 + +0.1 324,633 + -0.5 324,633 + -1.5 324,645 - -1.8 324,646 + +1.8 324,736 - -2.4 324,780 - MMJJ_RS01675 0.12 +0.1 324,808 - MMJJ_RS01675 0.15 -3.9 324,837 - MMJJ_RS01675 0.18 -1.6 324,840 - MMJJ_RS01675 0.19 -2.6 324,841 + MMJJ_RS01675 0.19 -0.8 324,841 + MMJJ_RS01675 0.19 -1.7 324,851 - MMJJ_RS01675 0.20 +0.1 324,851 - MMJJ_RS01675 0.20 -1.5 324,851 - MMJJ_RS01675 0.20 +0.6 324,852 + MMJJ_RS01675 0.20 -1.3 324,852 + MMJJ_RS01675 0.20 -0.3 324,869 - MMJJ_RS01675 0.22 -0.3 324,869 - MMJJ_RS01675 0.22 -2.2 324,869 - MMJJ_RS01675 0.22 -1.5 324,869 - MMJJ_RS01675 0.22 -2.1 324,869 - MMJJ_RS01675 0.22 -1.2 324,869 - MMJJ_RS01675 0.22 -1.9 324,869 - MMJJ_RS01675 0.22 -0.5 324,870 + MMJJ_RS01675 0.22 -2.1 324,876 - MMJJ_RS01675 0.22 +0.1 324,876 - MMJJ_RS01675 0.22 -1.5 324,876 - MMJJ_RS01675 0.22 -2.9 324,876 - MMJJ_RS01675 0.22 -4.4 324,877 + MMJJ_RS01675 0.23 -3.2 324,877 + MMJJ_RS01675 0.23 -2.9 324,877 + MMJJ_RS01675 0.23 -0.8 324,882 - MMJJ_RS01675 0.23 -1.0 324,891 - MMJJ_RS01675 0.24 +0.6 324,891 - MMJJ_RS01675 0.24 -2.6 324,892 + MMJJ_RS01675 0.24 -0.2 324,892 + MMJJ_RS01675 0.24 -1.6 324,899 - MMJJ_RS01675 0.25 -2.0 324,899 - MMJJ_RS01675 0.25 -0.1 324,904 + MMJJ_RS01675 0.25 -1.9 324,904 + MMJJ_RS01675 0.25 -3.8 324,948 - MMJJ_RS01675 0.30 +1.2 324,990 - MMJJ_RS01675 0.34 -2.0 324,990 - MMJJ_RS01675 0.34 -2.5 324,991 + -1.2 324,991 + MMJJ_RS01675 0.35 +2.6 324,996 - +0.3 324,996 - MMJJ_RS01675 0.35 -3.1 324,997 + MMJJ_RS01675 0.35 -0.3 324,997 + MMJJ_RS01675 0.35 -4.2 325,011 - MMJJ_RS01675 0.37 -3.4 325,011 - MMJJ_RS01675 0.37 -2.2 325,011 - MMJJ_RS01675 0.37 +1.8 325,012 + MMJJ_RS01675 0.37 -1.2 325,017 + MMJJ_RS01675 0.37 -3.7 325,051 - MMJJ_RS01675 0.41 +0.2 325,051 - MMJJ_RS01675 0.41 -0.2 325,051 - MMJJ_RS01675 0.41 -1.2 325,051 - -0.2 325,051 - MMJJ_RS01675 0.41 +0.4 325,051 - MMJJ_RS01675 0.41 -2.7 325,051 - MMJJ_RS01675 0.41 -2.6 325,051 - MMJJ_RS01675 0.41 -1.0 325,052 + MMJJ_RS01675 0.41 -1.0 325,052 + MMJJ_RS01675 0.41 -0.1 325,052 + MMJJ_RS01675 0.41 -1.4 325,075 - MMJJ_RS01675 0.43 -3.6 325,128 - MMJJ_RS01675 0.49 -2.7 325,134 + MMJJ_RS01675 0.50 -2.4 325,134 + MMJJ_RS01675 0.50 +0.6 325,174 + MMJJ_RS01675 0.54 -0.2 325,174 + MMJJ_RS01675 0.54 -0.7 325,235 - MMJJ_RS01675 0.60 -4.0 325,236 + MMJJ_RS01675 0.61 +0.5 325,236 + MMJJ_RS01675 0.61 -0.7 325,267 - MMJJ_RS01675 0.64 -5.4 325,305 - MMJJ_RS01675 0.68 +0.4 325,306 + MMJJ_RS01675 0.68 -2.5 325,312 - MMJJ_RS01675 0.69 +0.9 325,329 + MMJJ_RS01675 0.70 -1.1 325,329 + MMJJ_RS01675 0.70 -4.5 325,373 - MMJJ_RS01675 0.75 -0.2 325,382 - MMJJ_RS01675 0.76 +0.3 325,382 - MMJJ_RS01675 0.76 +1.5 325,383 + MMJJ_RS01675 0.76 -1.7 325,383 + MMJJ_RS01675 0.76 -1.1 325,383 + MMJJ_RS01675 0.76 +1.6 325,383 + -1.0 325,383 + MMJJ_RS01675 0.76 -2.0 325,383 + MMJJ_RS01675 0.76 -0.2 325,389 - MMJJ_RS01675 0.77 -3.2 325,389 - MMJJ_RS01675 0.77 -0.7 325,390 + MMJJ_RS01675 0.77 -0.7 325,472 - MMJJ_RS01675 0.86 -2.0 325,484 - MMJJ_RS01675 0.87 +0.8 325,515 + -1.0 325,571 - -1.2 325,571 - -1.9 325,572 + +0.3 325,602 - -1.3 325,603 + -2.6 325,650 - +1.3 325,650 - -1.1 325,650 - +1.1 325,657 - -3.4 325,715 + MMJJ_RS01680 0.10 -3.9 325,722 - MMJJ_RS01680 0.11 -3.6 325,722 - MMJJ_RS01680 0.11 -1.2 325,736 + MMJJ_RS01680 0.13 -3.0 325,743 - MMJJ_RS01680 0.13 -3.8 325,743 - MMJJ_RS01680 0.13 -3.7 325,783 - MMJJ_RS01680 0.18 -1.5 325,980 - MMJJ_RS01680 0.41 -2.8 326,022 + MMJJ_RS01680 0.45 -0.8 326,028 + MMJJ_RS01680 0.46 -1.9 326,028 + MMJJ_RS01680 0.46 -2.0 326,121 - MMJJ_RS01680 0.57 -2.4 326,179 + MMJJ_RS01680 0.63 -2.8 326,190 - MMJJ_RS01680 0.64 -1.5 326,191 + MMJJ_RS01680 0.65 -3.6 326,195 + MMJJ_RS01680 0.65 -0.2 326,220 + MMJJ_RS01680 0.68 -4.1 326,270 + MMJJ_RS01680 0.74 -0.7 326,290 + MMJJ_RS01680 0.76 -4.0 326,319 - MMJJ_RS01680 0.79 -3.8 326,319 - MMJJ_RS01680 0.79 -2.1 326,320 + MMJJ_RS01680 0.79 -3.5 326,326 + MMJJ_RS01680 0.80 -1.5 326,357 - MMJJ_RS01680 0.84 +0.1 326,357 - MMJJ_RS01680 0.84 -1.0 326,410 - MMJJ_RS01680 0.90 -3.6 326,501 - +1.8 326,502 + +1.5 326,512 + -0.8 326,585 - +0.3 326,778 - MMJJ_RS01685 0.27 -1.0 327,161 - MMJJ_RS01685 0.65 -1.2 327,379 - -0.7
Or see this region's nucleotide sequence