Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01570 and MMJJ_RS01575 are separated by 48 nucleotides MMJJ_RS01575 and MMJJ_RS01580 overlap by 4 nucleotides MMJJ_RS01580 and MMJJ_RS01585 are separated by 204 nucleotides
MMJJ_RS01570: MMJJ_RS01570 - SulP family inorganic anion transporter, at 305,226 to 306,845
_RS01570
MMJJ_RS01575: MMJJ_RS01575 - hypothetical protein, at 306,894 to 307,124
_RS01575
MMJJ_RS01580: MMJJ_RS01580 - phosphoadenosine phosphosulfate reductase family protein, at 307,121 to 307,996
_RS01580
MMJJ_RS01585: MMJJ_RS01585 - type I restriction endonuclease, at 308,201 to 309,325
_RS01585
Position (kb) 307
308 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 306.174 kb on - strand, within MMJJ_RS01570 at 306.185 kb on + strand, within MMJJ_RS01570 at 306.194 kb on - strand, within MMJJ_RS01570 at 306.194 kb on - strand, within MMJJ_RS01570 at 306.195 kb on + strand, within MMJJ_RS01570 at 306.205 kb on - strand, within MMJJ_RS01570 at 306.219 kb on - strand, within MMJJ_RS01570 at 306.219 kb on - strand, within MMJJ_RS01570 at 306.224 kb on - strand, within MMJJ_RS01570 at 306.303 kb on - strand, within MMJJ_RS01570 at 306.303 kb on - strand, within MMJJ_RS01570 at 306.385 kb on + strand, within MMJJ_RS01570 at 306.434 kb on - strand, within MMJJ_RS01570 at 306.458 kb on - strand, within MMJJ_RS01570 at 306.459 kb on + strand, within MMJJ_RS01570 at 306.464 kb on - strand, within MMJJ_RS01570 at 306.464 kb on - strand, within MMJJ_RS01570 at 306.465 kb on + strand, within MMJJ_RS01570 at 306.474 kb on - strand, within MMJJ_RS01570 at 306.486 kb on + strand, within MMJJ_RS01570 at 306.506 kb on - strand, within MMJJ_RS01570 at 306.507 kb on + strand, within MMJJ_RS01570 at 306.593 kb on - strand, within MMJJ_RS01570 at 306.603 kb on - strand, within MMJJ_RS01570 at 306.628 kb on + strand, within MMJJ_RS01570 at 306.636 kb on - strand, within MMJJ_RS01570 at 306.650 kb on - strand, within MMJJ_RS01570 at 306.831 kb on - strand at 306.858 kb on - strand at 306.860 kb on - strand at 307.031 kb on - strand at 307.032 kb on + strand, within MMJJ_RS01575 at 307.168 kb on - strand at 307.168 kb on - strand at 307.169 kb on + strand at 307.170 kb on - strand at 307.172 kb on - strand at 307.173 kb on + strand at 307.173 kb on + strand at 307.173 kb on + strand at 307.241 kb on + strand, within MMJJ_RS01580 at 307.316 kb on - strand, within MMJJ_RS01580 at 307.374 kb on + strand, within MMJJ_RS01580 at 307.381 kb on - strand, within MMJJ_RS01580 at 307.382 kb on + strand, within MMJJ_RS01580 at 307.382 kb on + strand, within MMJJ_RS01580 at 307.382 kb on + strand, within MMJJ_RS01580 at 307.382 kb on + strand, within MMJJ_RS01580 at 307.382 kb on + strand, within MMJJ_RS01580 at 307.383 kb on - strand, within MMJJ_RS01580 at 307.384 kb on + strand, within MMJJ_RS01580 at 307.384 kb on + strand, within MMJJ_RS01580 at 307.387 kb on - strand, within MMJJ_RS01580 at 307.388 kb on + strand, within MMJJ_RS01580 at 307.388 kb on + strand, within MMJJ_RS01580 at 307.388 kb on + strand, within MMJJ_RS01580 at 307.405 kb on - strand, within MMJJ_RS01580 at 307.406 kb on + strand, within MMJJ_RS01580 at 307.407 kb on - strand, within MMJJ_RS01580 at 307.429 kb on - strand, within MMJJ_RS01580 at 307.429 kb on - strand, within MMJJ_RS01580 at 307.429 kb on - strand, within MMJJ_RS01580 at 307.429 kb on - strand, within MMJJ_RS01580 at 307.429 kb on - strand, within MMJJ_RS01580 at 307.430 kb on + strand, within MMJJ_RS01580 at 307.430 kb on + strand, within MMJJ_RS01580 at 307.430 kb on + strand, within MMJJ_RS01580 at 307.540 kb on - strand, within MMJJ_RS01580 at 307.541 kb on + strand, within MMJJ_RS01580 at 307.557 kb on + strand, within MMJJ_RS01580 at 307.661 kb on + strand, within MMJJ_RS01580 at 307.669 kb on - strand, within MMJJ_RS01580 at 307.706 kb on - strand, within MMJJ_RS01580 at 307.713 kb on + strand, within MMJJ_RS01580 at 307.747 kb on + strand, within MMJJ_RS01580 at 307.798 kb on + strand, within MMJJ_RS01580 at 308.004 kb on - strand at 308.010 kb on - strand at 308.011 kb on + strand at 308.011 kb on + strand at 308.013 kb on - strand at 308.013 kb on - strand at 308.014 kb on + strand at 308.014 kb on + strand at 308.018 kb on + strand at 308.018 kb on + strand at 308.018 kb on + strand at 308.022 kb on + strand at 308.031 kb on + strand at 308.031 kb on + strand at 308.034 kb on - strand at 308.035 kb on + strand at 308.035 kb on + strand at 308.038 kb on - strand at 308.038 kb on - strand at 308.038 kb on - strand at 308.038 kb on - strand at 308.038 kb on - strand at 308.038 kb on - strand at 308.039 kb on + strand at 308.039 kb on + strand at 308.042 kb on - strand at 308.042 kb on - strand at 308.043 kb on + strand at 308.043 kb on + strand at 308.052 kb on + strand at 308.058 kb on + strand at 308.064 kb on + strand at 308.110 kb on - strand at 308.111 kb on + strand at 308.111 kb on + strand at 308.112 kb on - strand at 308.113 kb on + strand at 308.123 kb on + strand at 308.138 kb on - strand at 308.140 kb on - strand at 308.141 kb on + strand at 308.141 kb on + strand at 308.169 kb on - strand at 308.170 kb on + strand at 308.170 kb on + strand at 308.173 kb on - strand at 308.173 kb on - strand at 308.179 kb on - strand at 308.180 kb on + strand at 308.209 kb on - strand at 308.210 kb on + strand at 308.236 kb on + strand at 308.259 kb on + strand at 308.266 kb on - strand at 308.278 kb on - strand at 308.295 kb on - strand at 308.328 kb on - strand, within MMJJ_RS01585 at 308.329 kb on + strand, within MMJJ_RS01585 at 308.332 kb on - strand, within MMJJ_RS01585 at 308.416 kb on - strand, within MMJJ_RS01585 at 308.434 kb on - strand, within MMJJ_RS01585 at 308.443 kb on - strand, within MMJJ_RS01585 at 308.452 kb on - strand, within MMJJ_RS01585 at 308.493 kb on + strand, within MMJJ_RS01585 at 308.493 kb on + strand, within MMJJ_RS01585 at 308.530 kb on + strand, within MMJJ_RS01585 at 308.533 kb on - strand, within MMJJ_RS01585 at 308.534 kb on + strand, within MMJJ_RS01585 at 308.547 kb on + strand, within MMJJ_RS01585 at 308.547 kb on + strand, within MMJJ_RS01585 at 308.548 kb on - strand, within MMJJ_RS01585 at 308.579 kb on + strand, within MMJJ_RS01585 at 308.793 kb on - strand, within MMJJ_RS01585 at 308.793 kb on - strand, within MMJJ_RS01585 at 308.860 kb on - strand, within MMJJ_RS01585 at 308.860 kb on - strand, within MMJJ_RS01585 at 308.861 kb on + strand, within MMJJ_RS01585 at 308.861 kb on + strand, within MMJJ_RS01585 at 308.864 kb on + strand, within MMJJ_RS01585 at 308.871 kb on - strand, within MMJJ_RS01585 at 308.875 kb on - strand, within MMJJ_RS01585 at 308.884 kb on - strand, within MMJJ_RS01585 at 308.884 kb on - strand, within MMJJ_RS01585 at 308.897 kb on + strand, within MMJJ_RS01585 at 308.926 kb on - strand, within MMJJ_RS01585 at 308.927 kb on + strand, within MMJJ_RS01585 at 308.985 kb on - strand, within MMJJ_RS01585 at 308.986 kb on + strand, within MMJJ_RS01585 at 308.986 kb on + strand, within MMJJ_RS01585 at 308.990 kb on + strand, within MMJJ_RS01585 at 308.990 kb on + strand, within MMJJ_RS01585 at 308.990 kb on + strand, within MMJJ_RS01585
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 306,174 - MMJJ_RS01570 0.59 -1.2 306,185 + MMJJ_RS01570 0.59 +0.4 306,194 - MMJJ_RS01570 0.60 +0.7 306,194 - MMJJ_RS01570 0.60 +0.0 306,195 + MMJJ_RS01570 0.60 -0.0 306,205 - MMJJ_RS01570 0.60 -2.2 306,219 - MMJJ_RS01570 0.61 -0.8 306,219 - MMJJ_RS01570 0.61 -0.4 306,224 - MMJJ_RS01570 0.62 -3.2 306,303 - MMJJ_RS01570 0.66 +0.8 306,303 - MMJJ_RS01570 0.66 -1.3 306,385 + MMJJ_RS01570 0.72 +1.0 306,434 - MMJJ_RS01570 0.75 -1.7 306,458 - MMJJ_RS01570 0.76 +0.0 306,459 + MMJJ_RS01570 0.76 +1.1 306,464 - MMJJ_RS01570 0.76 +1.4 306,464 - MMJJ_RS01570 0.76 -0.6 306,465 + MMJJ_RS01570 0.76 -0.5 306,474 - MMJJ_RS01570 0.77 -1.4 306,486 + MMJJ_RS01570 0.78 -5.0 306,506 - MMJJ_RS01570 0.79 +0.8 306,507 + MMJJ_RS01570 0.79 -0.2 306,593 - MMJJ_RS01570 0.84 +1.7 306,603 - MMJJ_RS01570 0.85 +0.6 306,628 + MMJJ_RS01570 0.87 -0.5 306,636 - MMJJ_RS01570 0.87 +0.9 306,650 - MMJJ_RS01570 0.88 +0.6 306,831 - -3.4 306,858 - -2.8 306,860 - +2.1 307,031 - -0.2 307,032 + MMJJ_RS01575 0.60 -0.6 307,168 - -1.1 307,168 - -2.6 307,169 + -0.6 307,170 - -2.3 307,172 - -0.4 307,173 + -0.2 307,173 + +0.9 307,173 + -2.5 307,241 + MMJJ_RS01580 0.14 -1.6 307,316 - MMJJ_RS01580 0.22 -2.1 307,374 + MMJJ_RS01580 0.29 -0.7 307,381 - MMJJ_RS01580 0.30 -1.0 307,382 + MMJJ_RS01580 0.30 -1.4 307,382 + MMJJ_RS01580 0.30 -2.0 307,382 + MMJJ_RS01580 0.30 -2.0 307,382 + MMJJ_RS01580 0.30 -1.2 307,382 + MMJJ_RS01580 0.30 -3.8 307,383 - MMJJ_RS01580 0.30 -2.5 307,384 + MMJJ_RS01580 0.30 -0.9 307,384 + MMJJ_RS01580 0.30 -1.2 307,387 - MMJJ_RS01580 0.30 -2.5 307,388 + MMJJ_RS01580 0.30 -1.8 307,388 + MMJJ_RS01580 0.30 -2.3 307,388 + MMJJ_RS01580 0.30 -2.3 307,405 - MMJJ_RS01580 0.32 -2.4 307,406 + MMJJ_RS01580 0.33 +2.6 307,407 - MMJJ_RS01580 0.33 -4.3 307,429 - MMJJ_RS01580 0.35 -2.1 307,429 - MMJJ_RS01580 0.35 -1.1 307,429 - MMJJ_RS01580 0.35 -1.1 307,429 - MMJJ_RS01580 0.35 -0.9 307,429 - MMJJ_RS01580 0.35 -0.9 307,430 + MMJJ_RS01580 0.35 -1.6 307,430 + MMJJ_RS01580 0.35 +0.1 307,430 + MMJJ_RS01580 0.35 -1.9 307,540 - MMJJ_RS01580 0.48 -2.7 307,541 + MMJJ_RS01580 0.48 -1.2 307,557 + MMJJ_RS01580 0.50 -3.4 307,661 + MMJJ_RS01580 0.62 -0.7 307,669 - MMJJ_RS01580 0.63 -1.6 307,706 - MMJJ_RS01580 0.67 -2.4 307,713 + MMJJ_RS01580 0.68 -0.7 307,747 + MMJJ_RS01580 0.71 -2.4 307,798 + MMJJ_RS01580 0.77 -3.5 308,004 - -1.4 308,010 - -2.4 308,011 + -0.9 308,011 + -1.6 308,013 - -0.7 308,013 - -3.5 308,014 + -3.1 308,014 + -2.4 308,018 + -0.3 308,018 + -0.6 308,018 + -2.7 308,022 + -4.5 308,031 + -1.1 308,031 + -1.6 308,034 - +3.0 308,035 + -0.5 308,035 + -2.1 308,038 - -0.2 308,038 - -0.4 308,038 - -3.9 308,038 - +0.1 308,038 - -0.6 308,038 - -0.7 308,039 + -3.0 308,039 + -1.9 308,042 - +1.5 308,042 - -0.2 308,043 + -3.8 308,043 + +0.3 308,052 + -4.1 308,058 + -1.8 308,064 + -0.3 308,110 - -1.1 308,111 + +0.6 308,111 + -3.5 308,112 - +1.5 308,113 + -0.1 308,123 + -1.1 308,138 - -0.2 308,140 - -0.2 308,141 + +0.2 308,141 + -0.1 308,169 - +0.3 308,170 + +0.5 308,170 + +1.5 308,173 - +1.7 308,173 - -1.2 308,179 - -0.2 308,180 + +0.2 308,209 - -0.6 308,210 + -1.4 308,236 + -4.2 308,259 + +1.4 308,266 - +1.2 308,278 - -0.4 308,295 - -0.1 308,328 - MMJJ_RS01585 0.11 +0.7 308,329 + MMJJ_RS01585 0.11 -0.4 308,332 - MMJJ_RS01585 0.12 +1.1 308,416 - MMJJ_RS01585 0.19 -1.4 308,434 - MMJJ_RS01585 0.21 +0.4 308,443 - MMJJ_RS01585 0.22 +0.8 308,452 - MMJJ_RS01585 0.22 -0.0 308,493 + MMJJ_RS01585 0.26 +1.4 308,493 + MMJJ_RS01585 0.26 -0.6 308,530 + MMJJ_RS01585 0.29 +0.2 308,533 - MMJJ_RS01585 0.30 +0.5 308,534 + MMJJ_RS01585 0.30 -2.1 308,547 + MMJJ_RS01585 0.31 +0.4 308,547 + MMJJ_RS01585 0.31 +1.4 308,548 - MMJJ_RS01585 0.31 -1.6 308,579 + MMJJ_RS01585 0.34 -3.8 308,793 - MMJJ_RS01585 0.53 -3.0 308,793 - MMJJ_RS01585 0.53 -0.2 308,860 - MMJJ_RS01585 0.59 -1.2 308,860 - MMJJ_RS01585 0.59 +1.2 308,861 + MMJJ_RS01585 0.59 -0.4 308,861 + MMJJ_RS01585 0.59 -0.8 308,864 + MMJJ_RS01585 0.59 -4.7 308,871 - MMJJ_RS01585 0.60 +1.3 308,875 - MMJJ_RS01585 0.60 +0.3 308,884 - MMJJ_RS01585 0.61 -2.2 308,884 - MMJJ_RS01585 0.61 -2.1 308,897 + MMJJ_RS01585 0.62 +1.2 308,926 - MMJJ_RS01585 0.64 -2.1 308,927 + MMJJ_RS01585 0.65 +1.4 308,985 - MMJJ_RS01585 0.70 -2.5 308,986 + MMJJ_RS01585 0.70 +1.8 308,986 + MMJJ_RS01585 0.70 +0.8 308,990 + MMJJ_RS01585 0.70 +0.8 308,990 + MMJJ_RS01585 0.70 +1.7 308,990 + MMJJ_RS01585 0.70 -2.0
Or see this region's nucleotide sequence