Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00930 and MMJJ_RS00935 are separated by 46 nucleotides MMJJ_RS00935 and MMJJ_RS00940 are separated by 11 nucleotides MMJJ_RS00940 and MMJJ_RS00945 are separated by 139 nucleotides MMJJ_RS00945 and MMJJ_RS00950 are separated by 104 nucleotides
MMJJ_RS00930: MMJJ_RS00930 - queuosine precursor transporter, at 180,447 to 181,055
_RS00930
MMJJ_RS00935: MMJJ_RS00935 - nickel pincer cofactor biosynthesis protein LarB, at 181,102 to 181,887
_RS00935
MMJJ_RS00940: MMJJ_RS00940 - aldo/keto reductase, at 181,899 to 183,092
_RS00940
MMJJ_RS00945: MMJJ_RS00945 - carboxymuconolactone decarboxylase family protein, at 183,232 to 183,963
_RS00945
MMJJ_RS00950: MMJJ_RS00950 - DUF111 family protein, at 184,068 to 184,787
_RS00950
Position (kb) 181
182
183
184 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 180.914 kb on + strand, within MMJJ_RS00930 at 180.995 kb on - strand at 181.001 kb on + strand at 181.022 kb on - strand at 181.023 kb on + strand at 181.024 kb on - strand at 181.024 kb on - strand at 181.025 kb on + strand at 181.026 kb on - strand at 181.027 kb on + strand at 181.091 kb on + strand at 181.091 kb on + strand at 181.091 kb on + strand at 181.116 kb on - strand at 181.116 kb on - strand at 181.117 kb on + strand at 181.193 kb on + strand, within MMJJ_RS00935 at 181.202 kb on + strand, within MMJJ_RS00935 at 181.378 kb on - strand, within MMJJ_RS00935 at 181.382 kb on + strand, within MMJJ_RS00935 at 181.430 kb on - strand, within MMJJ_RS00935 at 181.430 kb on - strand, within MMJJ_RS00935 at 181.430 kb on - strand, within MMJJ_RS00935 at 181.430 kb on - strand, within MMJJ_RS00935 at 181.431 kb on + strand, within MMJJ_RS00935 at 181.431 kb on + strand, within MMJJ_RS00935 at 181.431 kb on + strand, within MMJJ_RS00935 at 181.432 kb on - strand, within MMJJ_RS00935 at 181.433 kb on + strand, within MMJJ_RS00935 at 181.433 kb on + strand, within MMJJ_RS00935 at 181.486 kb on - strand, within MMJJ_RS00935 at 181.487 kb on + strand, within MMJJ_RS00935 at 181.495 kb on + strand, within MMJJ_RS00935 at 181.504 kb on + strand, within MMJJ_RS00935 at 181.513 kb on + strand, within MMJJ_RS00935 at 181.516 kb on - strand, within MMJJ_RS00935 at 181.516 kb on - strand, within MMJJ_RS00935 at 181.517 kb on + strand, within MMJJ_RS00935 at 181.525 kb on - strand, within MMJJ_RS00935 at 181.526 kb on + strand, within MMJJ_RS00935 at 181.558 kb on + strand, within MMJJ_RS00935 at 181.609 kb on + strand, within MMJJ_RS00935 at 181.633 kb on + strand, within MMJJ_RS00935 at 181.647 kb on - strand, within MMJJ_RS00935 at 181.647 kb on - strand, within MMJJ_RS00935 at 181.647 kb on - strand, within MMJJ_RS00935 at 181.657 kb on - strand, within MMJJ_RS00935 at 181.780 kb on + strand, within MMJJ_RS00935 at 181.798 kb on - strand, within MMJJ_RS00935 at 181.872 kb on - strand at 181.901 kb on + strand at 181.901 kb on + strand at 181.965 kb on - strand at 181.997 kb on - strand at 182.016 kb on - strand at 182.016 kb on - strand at 182.017 kb on + strand at 182.017 kb on + strand at 182.018 kb on - strand at 182.073 kb on - strand, within MMJJ_RS00940 at 182.073 kb on - strand, within MMJJ_RS00940 at 182.074 kb on + strand, within MMJJ_RS00940 at 182.074 kb on + strand, within MMJJ_RS00940 at 182.074 kb on + strand, within MMJJ_RS00940 at 182.074 kb on + strand, within MMJJ_RS00940 at 182.074 kb on + strand, within MMJJ_RS00940 at 182.075 kb on - strand, within MMJJ_RS00940 at 182.081 kb on - strand, within MMJJ_RS00940 at 182.081 kb on - strand, within MMJJ_RS00940 at 182.082 kb on + strand, within MMJJ_RS00940 at 182.131 kb on + strand, within MMJJ_RS00940 at 182.141 kb on + strand, within MMJJ_RS00940 at 182.141 kb on + strand, within MMJJ_RS00940 at 182.141 kb on + strand, within MMJJ_RS00940 at 182.187 kb on + strand, within MMJJ_RS00940 at 182.205 kb on + strand, within MMJJ_RS00940 at 182.211 kb on + strand, within MMJJ_RS00940 at 182.211 kb on + strand, within MMJJ_RS00940 at 182.231 kb on + strand, within MMJJ_RS00940 at 182.250 kb on - strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.251 kb on + strand, within MMJJ_RS00940 at 182.308 kb on + strand, within MMJJ_RS00940 at 182.308 kb on + strand, within MMJJ_RS00940 at 182.397 kb on - strand, within MMJJ_RS00940 at 182.415 kb on + strand, within MMJJ_RS00940 at 182.521 kb on - strand, within MMJJ_RS00940 at 182.522 kb on + strand, within MMJJ_RS00940 at 182.560 kb on + strand, within MMJJ_RS00940 at 182.563 kb on - strand, within MMJJ_RS00940 at 182.568 kb on + strand, within MMJJ_RS00940 at 182.570 kb on + strand, within MMJJ_RS00940 at 182.574 kb on - strand, within MMJJ_RS00940 at 182.574 kb on - strand, within MMJJ_RS00940 at 182.574 kb on - strand, within MMJJ_RS00940 at 182.574 kb on - strand, within MMJJ_RS00940 at 182.574 kb on - strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.575 kb on + strand, within MMJJ_RS00940 at 182.596 kb on - strand, within MMJJ_RS00940 at 182.664 kb on + strand, within MMJJ_RS00940 at 182.664 kb on + strand, within MMJJ_RS00940 at 182.698 kb on + strand, within MMJJ_RS00940 at 182.736 kb on + strand, within MMJJ_RS00940 at 182.749 kb on + strand, within MMJJ_RS00940 at 182.752 kb on - strand, within MMJJ_RS00940 at 182.762 kb on - strand, within MMJJ_RS00940 at 182.768 kb on - strand, within MMJJ_RS00940 at 182.768 kb on - strand, within MMJJ_RS00940 at 182.769 kb on + strand, within MMJJ_RS00940 at 182.805 kb on + strand, within MMJJ_RS00940 at 182.810 kb on - strand, within MMJJ_RS00940 at 182.826 kb on - strand, within MMJJ_RS00940 at 182.859 kb on + strand, within MMJJ_RS00940 at 183.021 kb on + strand at 183.024 kb on - strand at 183.110 kb on - strand at 183.111 kb on + strand at 183.113 kb on + strand at 183.126 kb on - strand at 183.150 kb on + strand at 183.166 kb on - strand at 183.166 kb on - strand at 183.166 kb on - strand at 183.171 kb on - strand at 183.172 kb on + strand at 183.200 kb on - strand at 183.200 kb on - strand at 183.200 kb on - strand at 183.200 kb on - strand at 183.201 kb on + strand at 183.201 kb on + strand at 183.201 kb on + strand at 183.201 kb on + strand at 183.206 kb on - strand at 183.210 kb on + strand at 183.215 kb on + strand at 183.234 kb on + strand at 183.234 kb on + strand at 183.253 kb on - strand at 183.289 kb on - strand at 183.310 kb on + strand, within MMJJ_RS00945 at 183.322 kb on - strand, within MMJJ_RS00945 at 183.323 kb on + strand, within MMJJ_RS00945 at 183.323 kb on + strand, within MMJJ_RS00945 at 183.325 kb on - strand, within MMJJ_RS00945 at 183.325 kb on - strand, within MMJJ_RS00945 at 183.363 kb on - strand, within MMJJ_RS00945 at 183.364 kb on + strand, within MMJJ_RS00945 at 183.364 kb on + strand, within MMJJ_RS00945 at 183.374 kb on + strand, within MMJJ_RS00945 at 183.407 kb on + strand, within MMJJ_RS00945 at 183.407 kb on + strand, within MMJJ_RS00945 at 183.408 kb on - strand, within MMJJ_RS00945 at 183.408 kb on - strand, within MMJJ_RS00945 at 183.458 kb on - strand, within MMJJ_RS00945 at 183.459 kb on + strand, within MMJJ_RS00945 at 183.459 kb on + strand, within MMJJ_RS00945 at 183.459 kb on + strand, within MMJJ_RS00945 at 183.488 kb on - strand, within MMJJ_RS00945 at 183.488 kb on - strand, within MMJJ_RS00945 at 183.502 kb on - strand, within MMJJ_RS00945 at 183.502 kb on - strand, within MMJJ_RS00945 at 183.502 kb on - strand, within MMJJ_RS00945 at 183.527 kb on - strand, within MMJJ_RS00945 at 183.528 kb on + strand, within MMJJ_RS00945 at 183.589 kb on + strand, within MMJJ_RS00945 at 183.620 kb on + strand, within MMJJ_RS00945 at 183.625 kb on - strand, within MMJJ_RS00945 at 183.631 kb on - strand, within MMJJ_RS00945 at 183.678 kb on + strand, within MMJJ_RS00945 at 183.678 kb on + strand, within MMJJ_RS00945 at 183.698 kb on - strand, within MMJJ_RS00945 at 183.698 kb on - strand, within MMJJ_RS00945 at 183.713 kb on - strand, within MMJJ_RS00945 at 183.713 kb on - strand, within MMJJ_RS00945 at 183.715 kb on - strand, within MMJJ_RS00945 at 183.715 kb on - strand, within MMJJ_RS00945 at 183.715 kb on - strand, within MMJJ_RS00945 at 183.715 kb on - strand, within MMJJ_RS00945 at 183.716 kb on + strand, within MMJJ_RS00945 at 183.716 kb on + strand, within MMJJ_RS00945 at 183.718 kb on - strand, within MMJJ_RS00945 at 183.718 kb on - strand, within MMJJ_RS00945 at 183.719 kb on + strand, within MMJJ_RS00945 at 183.719 kb on + strand, within MMJJ_RS00945 at 183.735 kb on - strand, within MMJJ_RS00945 at 183.735 kb on - strand, within MMJJ_RS00945 at 183.742 kb on - strand, within MMJJ_RS00945 at 183.773 kb on + strand, within MMJJ_RS00945 at 183.777 kb on - strand, within MMJJ_RS00945 at 183.778 kb on + strand, within MMJJ_RS00945 at 183.861 kb on - strand, within MMJJ_RS00945 at 183.862 kb on + strand, within MMJJ_RS00945 at 183.914 kb on + strand at 183.918 kb on - strand at 183.918 kb on - strand at 183.964 kb on + strand at 184.025 kb on - strand at 184.028 kb on - strand at 184.028 kb on - strand at 184.029 kb on + strand at 184.029 kb on + strand at 184.031 kb on + strand at 184.037 kb on - strand at 184.046 kb on + strand at 184.046 kb on + strand at 184.046 kb on + strand at 184.053 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 180,914 + MMJJ_RS00930 0.77 +0.6 180,995 - +0.0 181,001 + +0.1 181,022 - -0.7 181,023 + -2.1 181,024 - -4.3 181,024 - -0.7 181,025 + -1.7 181,026 - -0.8 181,027 + -1.6 181,091 + +0.7 181,091 + +1.1 181,091 + +0.5 181,116 - +0.1 181,116 - +2.2 181,117 + -3.5 181,193 + MMJJ_RS00935 0.12 -1.3 181,202 + MMJJ_RS00935 0.13 -0.3 181,378 - MMJJ_RS00935 0.35 -0.5 181,382 + MMJJ_RS00935 0.36 -0.8 181,430 - MMJJ_RS00935 0.42 -0.7 181,430 - MMJJ_RS00935 0.42 -2.6 181,430 - MMJJ_RS00935 0.42 -1.2 181,430 - MMJJ_RS00935 0.42 -1.1 181,431 + MMJJ_RS00935 0.42 -3.9 181,431 + MMJJ_RS00935 0.42 -0.6 181,431 + MMJJ_RS00935 0.42 -2.1 181,432 - MMJJ_RS00935 0.42 -3.4 181,433 + MMJJ_RS00935 0.42 -0.1 181,433 + MMJJ_RS00935 0.42 -2.4 181,486 - MMJJ_RS00935 0.49 +0.7 181,487 + MMJJ_RS00935 0.49 +0.1 181,495 + MMJJ_RS00935 0.50 -0.2 181,504 + MMJJ_RS00935 0.51 -4.0 181,513 + MMJJ_RS00935 0.52 -2.3 181,516 - MMJJ_RS00935 0.53 -1.5 181,516 - MMJJ_RS00935 0.53 -3.3 181,517 + MMJJ_RS00935 0.53 -2.6 181,525 - MMJJ_RS00935 0.54 -1.9 181,526 + MMJJ_RS00935 0.54 -3.3 181,558 + MMJJ_RS00935 0.58 -3.1 181,609 + MMJJ_RS00935 0.65 -1.1 181,633 + MMJJ_RS00935 0.68 +0.2 181,647 - MMJJ_RS00935 0.69 -0.4 181,647 - MMJJ_RS00935 0.69 -2.3 181,647 - MMJJ_RS00935 0.69 -1.7 181,657 - MMJJ_RS00935 0.71 -2.5 181,780 + MMJJ_RS00935 0.86 -0.0 181,798 - MMJJ_RS00935 0.89 -3.3 181,872 - -4.5 181,901 + +2.1 181,901 + +4.4 181,965 - -0.9 181,997 - +1.4 182,016 - +0.5 182,016 - +3.0 182,017 + +0.3 182,017 + +3.4 182,018 - -1.3 182,073 - MMJJ_RS00940 0.15 +2.6 182,073 - MMJJ_RS00940 0.15 +0.8 182,074 + MMJJ_RS00940 0.15 +1.4 182,074 + MMJJ_RS00940 0.15 +1.3 182,074 + MMJJ_RS00940 0.15 +0.6 182,074 + MMJJ_RS00940 0.15 +1.4 182,074 + MMJJ_RS00940 0.15 +0.1 182,075 - MMJJ_RS00940 0.15 +0.6 182,081 - MMJJ_RS00940 0.15 +0.7 182,081 - MMJJ_RS00940 0.15 +2.0 182,082 + MMJJ_RS00940 0.15 +2.0 182,131 + MMJJ_RS00940 0.19 +3.4 182,141 + MMJJ_RS00940 0.20 +4.2 182,141 + MMJJ_RS00940 0.20 +2.4 182,141 + MMJJ_RS00940 0.20 +3.1 182,187 + MMJJ_RS00940 0.24 +2.6 182,205 + MMJJ_RS00940 0.26 +1.7 182,211 + MMJJ_RS00940 0.26 +2.0 182,211 + MMJJ_RS00940 0.26 +1.3 182,231 + MMJJ_RS00940 0.28 +2.1 182,250 - MMJJ_RS00940 0.29 -1.3 182,251 + MMJJ_RS00940 0.29 +0.1 182,251 + MMJJ_RS00940 0.29 +0.3 182,251 + MMJJ_RS00940 0.29 +0.1 182,251 + MMJJ_RS00940 0.29 +2.3 182,251 + MMJJ_RS00940 0.29 +2.5 182,251 + MMJJ_RS00940 0.29 +1.3 182,308 + MMJJ_RS00940 0.34 -1.3 182,308 + MMJJ_RS00940 0.34 +1.2 182,397 - MMJJ_RS00940 0.42 +2.2 182,415 + MMJJ_RS00940 0.43 +1.4 182,521 - MMJJ_RS00940 0.52 -0.3 182,522 + MMJJ_RS00940 0.52 +2.3 182,560 + MMJJ_RS00940 0.55 +0.4 182,563 - MMJJ_RS00940 0.56 +0.2 182,568 + MMJJ_RS00940 0.56 +2.1 182,570 + MMJJ_RS00940 0.56 -1.8 182,574 - MMJJ_RS00940 0.57 +1.6 182,574 - MMJJ_RS00940 0.57 -2.2 182,574 - MMJJ_RS00940 0.57 +0.2 182,574 - MMJJ_RS00940 0.57 +1.3 182,574 - MMJJ_RS00940 0.57 +0.7 182,575 + MMJJ_RS00940 0.57 +1.8 182,575 + MMJJ_RS00940 0.57 -1.5 182,575 + MMJJ_RS00940 0.57 +1.9 182,575 + MMJJ_RS00940 0.57 +1.2 182,575 + MMJJ_RS00940 0.57 +1.1 182,575 + MMJJ_RS00940 0.57 +0.6 182,575 + MMJJ_RS00940 0.57 -1.5 182,596 - MMJJ_RS00940 0.58 -0.9 182,664 + MMJJ_RS00940 0.64 +0.4 182,664 + MMJJ_RS00940 0.64 +0.6 182,698 + MMJJ_RS00940 0.67 -0.3 182,736 + MMJJ_RS00940 0.70 +2.8 182,749 + MMJJ_RS00940 0.71 -0.6 182,752 - MMJJ_RS00940 0.71 -3.9 182,762 - MMJJ_RS00940 0.72 -1.1 182,768 - MMJJ_RS00940 0.73 +0.2 182,768 - MMJJ_RS00940 0.73 +0.6 182,769 + MMJJ_RS00940 0.73 -4.6 182,805 + MMJJ_RS00940 0.76 +0.7 182,810 - MMJJ_RS00940 0.76 +0.8 182,826 - MMJJ_RS00940 0.78 +0.9 182,859 + MMJJ_RS00940 0.80 -0.6 183,021 + +1.5 183,024 - +1.4 183,110 - +1.4 183,111 + -0.5 183,113 + +2.2 183,126 - +0.4 183,150 + -1.3 183,166 - -1.6 183,166 - -1.9 183,166 - -4.3 183,171 - +1.7 183,172 + -1.2 183,200 - -2.7 183,200 - -2.9 183,200 - +0.5 183,200 - -3.6 183,201 + -2.1 183,201 + -0.7 183,201 + -0.6 183,201 + -1.5 183,206 - -0.0 183,210 + +0.3 183,215 + +0.3 183,234 + -1.2 183,234 + -1.0 183,253 - +0.7 183,289 - -1.1 183,310 + MMJJ_RS00945 0.11 -0.8 183,322 - MMJJ_RS00945 0.12 -1.1 183,323 + MMJJ_RS00945 0.12 -3.4 183,323 + MMJJ_RS00945 0.12 -1.1 183,325 - MMJJ_RS00945 0.13 -0.2 183,325 - MMJJ_RS00945 0.13 -1.7 183,363 - MMJJ_RS00945 0.18 -1.3 183,364 + MMJJ_RS00945 0.18 +1.3 183,364 + MMJJ_RS00945 0.18 +1.6 183,374 + MMJJ_RS00945 0.19 +0.2 183,407 + MMJJ_RS00945 0.24 -0.1 183,407 + MMJJ_RS00945 0.24 -0.7 183,408 - MMJJ_RS00945 0.24 -0.1 183,408 - MMJJ_RS00945 0.24 +1.1 183,458 - MMJJ_RS00945 0.31 -1.0 183,459 + MMJJ_RS00945 0.31 +1.6 183,459 + MMJJ_RS00945 0.31 +0.9 183,459 + MMJJ_RS00945 0.31 +0.7 183,488 - MMJJ_RS00945 0.35 +1.8 183,488 - MMJJ_RS00945 0.35 +0.8 183,502 - MMJJ_RS00945 0.37 -0.5 183,502 - MMJJ_RS00945 0.37 +0.4 183,502 - MMJJ_RS00945 0.37 +0.5 183,527 - MMJJ_RS00945 0.40 -0.2 183,528 + MMJJ_RS00945 0.40 +2.1 183,589 + MMJJ_RS00945 0.49 -0.3 183,620 + MMJJ_RS00945 0.53 -1.6 183,625 - MMJJ_RS00945 0.54 +0.9 183,631 - MMJJ_RS00945 0.55 +0.9 183,678 + MMJJ_RS00945 0.61 -0.0 183,678 + MMJJ_RS00945 0.61 +0.2 183,698 - MMJJ_RS00945 0.64 +3.5 183,698 - MMJJ_RS00945 0.64 +0.1 183,713 - MMJJ_RS00945 0.66 +0.8 183,713 - MMJJ_RS00945 0.66 -1.5 183,715 - MMJJ_RS00945 0.66 +1.5 183,715 - MMJJ_RS00945 0.66 +0.2 183,715 - MMJJ_RS00945 0.66 +0.1 183,715 - MMJJ_RS00945 0.66 -0.1 183,716 + MMJJ_RS00945 0.66 -0.7 183,716 + MMJJ_RS00945 0.66 -0.2 183,718 - MMJJ_RS00945 0.66 -4.4 183,718 - MMJJ_RS00945 0.66 -0.3 183,719 + MMJJ_RS00945 0.67 +2.4 183,719 + MMJJ_RS00945 0.67 -1.9 183,735 - MMJJ_RS00945 0.69 +1.4 183,735 - MMJJ_RS00945 0.69 +2.1 183,742 - MMJJ_RS00945 0.70 -0.4 183,773 + MMJJ_RS00945 0.74 +0.7 183,777 - MMJJ_RS00945 0.74 -0.9 183,778 + MMJJ_RS00945 0.75 +0.1 183,861 - MMJJ_RS00945 0.86 -2.1 183,862 + MMJJ_RS00945 0.86 -0.5 183,914 + -0.7 183,918 - +0.7 183,918 - +1.3 183,964 + -0.7 184,025 - -1.0 184,028 - +0.5 184,028 - -0.8 184,029 + -0.8 184,029 + -3.8 184,031 + -1.5 184,037 - +1.0 184,046 + -1.5 184,046 + +0.5 184,046 + -2.4 184,053 + +0.6
Or see this region's nucleotide sequence