Experiment: NLDM_metabolites (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02165 and EX31_RS02170 are separated by 200 nucleotides EX31_RS02170 and EX31_RS02175 are separated by 93 nucleotides EX31_RS02175 and EX31_RS02180 are separated by 18 nucleotides EX31_RS02180 and EX31_RS02185 are separated by 278 nucleotides
EX31_RS02165: EX31_RS02165 - hypothetical protein, at 472,095 to 472,586
_RS02165
EX31_RS02170: EX31_RS02170 - porin, at 472,787 to 473,353
_RS02170
EX31_RS02175: EX31_RS02175 - peptide-methionine (S)-S-oxide reductase MsrA, at 473,447 to 474,175
_RS02175
EX31_RS02180: EX31_RS02180 - peptide-methionine (R)-S-oxide reductase MsrB, at 474,194 to 474,700
_RS02180
EX31_RS02185: EX31_RS02185 - glycoside hydrolase family 31 protein, at 474,979 to 477,018
_RS02185
Position (kb)
473
474
475 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 472.536 kb on + strand, within EX31_RS02165 at 472.536 kb on + strand, within EX31_RS02165 at 472.536 kb on + strand, within EX31_RS02165 at 472.536 kb on + strand, within EX31_RS02165 at 472.537 kb on - strand at 472.537 kb on - strand at 472.537 kb on - strand at 472.550 kb on + strand at 472.554 kb on + strand at 472.651 kb on + strand at 472.651 kb on + strand at 472.668 kb on + strand at 472.670 kb on + strand at 472.702 kb on - strand at 472.776 kb on + strand at 472.781 kb on - strand at 472.792 kb on + strand at 472.795 kb on + strand at 472.795 kb on + strand at 472.795 kb on + strand at 472.795 kb on + strand at 472.796 kb on - strand at 472.841 kb on - strand at 472.841 kb on - strand at 473.011 kb on + strand, within EX31_RS02170 at 473.039 kb on - strand, within EX31_RS02170 at 473.039 kb on - strand, within EX31_RS02170 at 473.060 kb on + strand, within EX31_RS02170 at 473.060 kb on + strand, within EX31_RS02170 at 473.061 kb on - strand, within EX31_RS02170 at 473.062 kb on + strand, within EX31_RS02170 at 473.063 kb on - strand, within EX31_RS02170 at 473.074 kb on + strand, within EX31_RS02170 at 473.074 kb on + strand, within EX31_RS02170 at 473.083 kb on + strand, within EX31_RS02170 at 473.150 kb on + strand, within EX31_RS02170 at 473.151 kb on - strand, within EX31_RS02170 at 473.314 kb on + strand at 473.327 kb on + strand at 473.327 kb on + strand at 473.328 kb on - strand at 473.356 kb on + strand at 473.494 kb on - strand at 473.537 kb on - strand, within EX31_RS02175 at 473.538 kb on + strand, within EX31_RS02175 at 473.539 kb on - strand, within EX31_RS02175 at 473.539 kb on - strand, within EX31_RS02175 at 473.544 kb on + strand, within EX31_RS02175 at 473.638 kb on - strand, within EX31_RS02175 at 473.667 kb on + strand, within EX31_RS02175 at 473.668 kb on - strand, within EX31_RS02175 at 473.810 kb on + strand, within EX31_RS02175 at 473.881 kb on - strand, within EX31_RS02175 at 473.939 kb on + strand, within EX31_RS02175 at 473.940 kb on - strand, within EX31_RS02175 at 474.196 kb on - strand at 474.479 kb on + strand, within EX31_RS02180 at 474.492 kb on + strand, within EX31_RS02180 at 474.597 kb on - strand, within EX31_RS02180 at 474.739 kb on + strand at 474.907 kb on + strand at 474.908 kb on - strand at 474.908 kb on - strand at 474.940 kb on + strand at 474.941 kb on - strand at 474.941 kb on - strand at 475.148 kb on + strand at 475.148 kb on + strand at 475.149 kb on - strand at 475.149 kb on - strand at 475.149 kb on - strand at 475.170 kb on + strand at 475.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_metabolites (C) remove 472,536 + EX31_RS02165 0.90 +0.8 472,536 + EX31_RS02165 0.90 -1.0 472,536 + EX31_RS02165 0.90 +1.9 472,536 + EX31_RS02165 0.90 -1.3 472,537 - +1.3 472,537 - +0.4 472,537 - +0.0 472,550 + +0.8 472,554 + +1.0 472,651 + +0.3 472,651 + -1.3 472,668 + +1.8 472,670 + -1.4 472,702 - -2.6 472,776 + +0.0 472,781 - +0.5 472,792 + -1.3 472,795 + -0.6 472,795 + +0.0 472,795 + -0.6 472,795 + +0.8 472,796 - +0.2 472,841 - +0.7 472,841 - +0.6 473,011 + EX31_RS02170 0.40 +0.3 473,039 - EX31_RS02170 0.44 +0.6 473,039 - EX31_RS02170 0.44 -1.3 473,060 + EX31_RS02170 0.48 -0.4 473,060 + EX31_RS02170 0.48 +0.7 473,061 - EX31_RS02170 0.48 -0.6 473,062 + EX31_RS02170 0.49 -1.6 473,063 - EX31_RS02170 0.49 +0.9 473,074 + EX31_RS02170 0.51 +0.7 473,074 + EX31_RS02170 0.51 -1.1 473,083 + EX31_RS02170 0.52 -2.3 473,150 + EX31_RS02170 0.64 +1.8 473,151 - EX31_RS02170 0.64 -2.3 473,314 + +0.4 473,327 + +1.0 473,327 + +1.0 473,328 - +0.4 473,356 + -0.9 473,494 - -0.6 473,537 - EX31_RS02175 0.12 -1.0 473,538 + EX31_RS02175 0.12 +0.6 473,539 - EX31_RS02175 0.13 -0.8 473,539 - EX31_RS02175 0.13 -2.1 473,544 + EX31_RS02175 0.13 -0.3 473,638 - EX31_RS02175 0.26 -1.3 473,667 + EX31_RS02175 0.30 -0.1 473,668 - EX31_RS02175 0.30 -0.6 473,810 + EX31_RS02175 0.50 +0.0 473,881 - EX31_RS02175 0.60 -0.3 473,939 + EX31_RS02175 0.67 -0.6 473,940 - EX31_RS02175 0.68 +0.0 474,196 - -2.0 474,479 + EX31_RS02180 0.56 -0.2 474,492 + EX31_RS02180 0.59 +0.0 474,597 - EX31_RS02180 0.79 +1.0 474,739 + +1.0 474,907 + +1.3 474,908 - -0.2 474,908 - -1.0 474,940 + +3.1 474,941 - +1.6 474,941 - -1.3 475,148 + -0.8 475,148 + +0.0 475,149 - +0.6 475,149 - -1.1 475,149 - +0.8 475,170 + +0.9 475,171 - +0.6
Or see this region's nucleotide sequence