Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29015 and RR42_RS29020 are separated by 60 nucleotides RR42_RS29020 and RR42_RS29025 are separated by 180 nucleotides RR42_RS29025 and RR42_RS29030 are separated by 188 nucleotides RR42_RS29030 and RR42_RS29035 are separated by 132 nucleotides
RR42_RS29015: RR42_RS29015 - IQ calmodulin-binding motif-containing protein, at 1,865,746 to 1,866,765
_RS29015
RR42_RS29020: RR42_RS29020 - hypothetical protein, at 1,866,826 to 1,867,176
_RS29020
RR42_RS29025: RR42_RS29025 - hypothetical protein, at 1,867,357 to 1,867,539
_RS29025
RR42_RS29030: RR42_RS29030 - metal-dependent hydrolase, at 1,867,728 to 1,868,690
_RS29030
RR42_RS29035: RR42_RS29035 - acyl-CoA synthetase, at 1,868,823 to 1,870,505
_RS29035
Position (kb)
1867
1868
1869 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1866.763 kb on + strand at 1866.886 kb on + strand, within RR42_RS29020 at 1866.905 kb on + strand, within RR42_RS29020 at 1866.905 kb on + strand, within RR42_RS29020 at 1867.381 kb on + strand, within RR42_RS29025 at 1867.601 kb on + strand at 1867.602 kb on - strand at 1867.602 kb on - strand at 1867.602 kb on - strand at 1867.637 kb on - strand at 1867.827 kb on + strand, within RR42_RS29030 at 1867.827 kb on + strand, within RR42_RS29030 at 1867.827 kb on + strand, within RR42_RS29030 at 1867.827 kb on + strand, within RR42_RS29030 at 1867.828 kb on - strand, within RR42_RS29030 at 1867.828 kb on - strand, within RR42_RS29030 at 1867.828 kb on - strand, within RR42_RS29030 at 1867.980 kb on + strand, within RR42_RS29030 at 1867.980 kb on + strand, within RR42_RS29030 at 1868.046 kb on + strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030 at 1868.047 kb on - strand, within RR42_RS29030 at 1868.406 kb on + strand, within RR42_RS29030 at 1868.406 kb on + strand, within RR42_RS29030 at 1868.407 kb on - strand, within RR42_RS29030 at 1868.623 kb on - strand at 1868.644 kb on + strand at 1868.645 kb on - strand at 1868.645 kb on - strand at 1868.645 kb on - strand at 1868.688 kb on + strand at 1868.688 kb on + strand at 1868.784 kb on + strand at 1868.784 kb on + strand at 1868.784 kb on + strand at 1868.784 kb on + strand at 1868.785 kb on - strand at 1868.859 kb on + strand at 1868.859 kb on + strand at 1868.860 kb on - strand at 1868.860 kb on - strand at 1868.898 kb on - strand at 1868.898 kb on - strand at 1868.908 kb on + strand at 1868.961 kb on + strand at 1868.961 kb on + strand at 1868.961 kb on + strand at 1868.962 kb on - strand at 1868.962 kb on - strand at 1868.982 kb on + strand at 1868.982 kb on + strand at 1868.983 kb on - strand at 1869.067 kb on - strand, within RR42_RS29035 at 1869.199 kb on + strand, within RR42_RS29035 at 1869.199 kb on + strand, within RR42_RS29035 at 1869.199 kb on + strand, within RR42_RS29035 at 1869.199 kb on + strand, within RR42_RS29035 at 1869.222 kb on + strand, within RR42_RS29035 at 1869.417 kb on + strand, within RR42_RS29035 at 1869.418 kb on - strand, within RR42_RS29035 at 1869.478 kb on - strand, within RR42_RS29035 at 1869.478 kb on - strand, within RR42_RS29035 at 1869.478 kb on - strand, within RR42_RS29035 at 1869.552 kb on + strand, within RR42_RS29035 at 1869.614 kb on + strand, within RR42_RS29035
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 1,866,763 + +0.4 1,866,886 + RR42_RS29020 0.17 +0.2 1,866,905 + RR42_RS29020 0.23 +0.8 1,866,905 + RR42_RS29020 0.23 +0.5 1,867,381 + RR42_RS29025 0.13 -2.1 1,867,601 + +0.4 1,867,602 - +0.2 1,867,602 - +0.3 1,867,602 - +1.1 1,867,637 - +0.7 1,867,827 + RR42_RS29030 0.10 +0.2 1,867,827 + RR42_RS29030 0.10 -3.2 1,867,827 + RR42_RS29030 0.10 -0.8 1,867,827 + RR42_RS29030 0.10 -0.1 1,867,828 - RR42_RS29030 0.10 +0.4 1,867,828 - RR42_RS29030 0.10 -0.5 1,867,828 - RR42_RS29030 0.10 +0.2 1,867,980 + RR42_RS29030 0.26 +0.4 1,867,980 + RR42_RS29030 0.26 -0.3 1,868,046 + RR42_RS29030 0.33 -0.6 1,868,047 - RR42_RS29030 0.33 +2.2 1,868,047 - RR42_RS29030 0.33 +1.2 1,868,047 - RR42_RS29030 0.33 +0.6 1,868,047 - RR42_RS29030 0.33 -0.8 1,868,406 + RR42_RS29030 0.70 -0.7 1,868,406 + RR42_RS29030 0.70 +0.1 1,868,407 - RR42_RS29030 0.71 -0.7 1,868,623 - +0.5 1,868,644 + -1.1 1,868,645 - -0.5 1,868,645 - -0.6 1,868,645 - +0.6 1,868,688 + +0.7 1,868,688 + -0.0 1,868,784 + -0.7 1,868,784 + -0.1 1,868,784 + +1.8 1,868,784 + +1.5 1,868,785 - -0.4 1,868,859 + -0.0 1,868,859 + +1.6 1,868,860 - +1.0 1,868,860 - +0.2 1,868,898 - +0.0 1,868,898 - -0.3 1,868,908 + +0.3 1,868,961 + -1.8 1,868,961 + -0.6 1,868,961 + -1.3 1,868,962 - +1.6 1,868,962 - +0.0 1,868,982 + -2.0 1,868,982 + -0.5 1,868,983 - -0.7 1,869,067 - RR42_RS29035 0.14 +0.8 1,869,199 + RR42_RS29035 0.22 +0.4 1,869,199 + RR42_RS29035 0.22 +0.1 1,869,199 + RR42_RS29035 0.22 +0.0 1,869,199 + RR42_RS29035 0.22 +0.1 1,869,222 + RR42_RS29035 0.24 +0.7 1,869,417 + RR42_RS29035 0.35 +0.1 1,869,418 - RR42_RS29035 0.35 -0.9 1,869,478 - RR42_RS29035 0.39 +0.8 1,869,478 - RR42_RS29035 0.39 +0.4 1,869,478 - RR42_RS29035 0.39 -0.9 1,869,552 + RR42_RS29035 0.43 +0.5 1,869,614 + RR42_RS29035 0.47 +1.0
Or see this region's nucleotide sequence