Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23035 and RR42_RS23040 are separated by 398 nucleotides RR42_RS23040 and RR42_RS23045 are separated by 165 nucleotides RR42_RS23045 and RR42_RS23050 are separated by 60 nucleotides
RR42_RS23035: RR42_RS23035 - sporulation protein, at 502,881 to 503,831
_RS23035
RR42_RS23040: RR42_RS23040 - hypothetical protein, at 504,230 to 504,514
_RS23040
RR42_RS23045: RR42_RS23045 - LysR family transcriptional regulator, at 504,680 to 505,585
_RS23045
RR42_RS23050: RR42_RS23050 - 4-oxalomesaconate tautomerase, at 505,646 to 506,725
_RS23050
Position (kb) 504
505
506 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 503.771 kb on + strand at 503.900 kb on + strand at 503.906 kb on + strand at 503.907 kb on - strand at 503.932 kb on + strand at 503.957 kb on + strand at 503.958 kb on - strand at 503.995 kb on - strand at 504.017 kb on + strand at 504.034 kb on + strand at 504.035 kb on - strand at 504.035 kb on - strand at 504.035 kb on - strand at 504.084 kb on - strand at 504.084 kb on - strand at 504.085 kb on + strand at 504.085 kb on + strand at 504.085 kb on + strand at 504.085 kb on + strand at 504.086 kb on - strand at 504.087 kb on + strand at 504.088 kb on - strand at 504.088 kb on - strand at 504.175 kb on + strand at 504.176 kb on - strand at 504.214 kb on - strand at 504.222 kb on + strand at 504.223 kb on - strand at 504.239 kb on - strand at 504.473 kb on + strand, within RR42_RS23040 at 504.478 kb on + strand, within RR42_RS23040 at 504.536 kb on + strand at 504.536 kb on + strand at 504.536 kb on + strand at 504.539 kb on + strand at 504.540 kb on - strand at 504.540 kb on - strand at 504.543 kb on + strand at 504.543 kb on + strand at 504.543 kb on + strand at 504.547 kb on + strand at 504.547 kb on + strand at 504.547 kb on + strand at 504.548 kb on - strand at 504.548 kb on - strand at 504.586 kb on - strand at 504.586 kb on - strand at 504.586 kb on - strand at 504.635 kb on - strand at 504.682 kb on - strand at 505.028 kb on + strand, within RR42_RS23045 at 505.028 kb on + strand, within RR42_RS23045 at 505.200 kb on - strand, within RR42_RS23045 at 505.222 kb on - strand, within RR42_RS23045 at 505.261 kb on - strand, within RR42_RS23045 at 505.261 kb on - strand, within RR42_RS23045 at 505.264 kb on - strand, within RR42_RS23045 at 505.264 kb on - strand, within RR42_RS23045 at 505.613 kb on + strand at 505.613 kb on + strand at 505.614 kb on - strand at 505.614 kb on - strand at 505.628 kb on - strand at 505.669 kb on - strand at 505.749 kb on + strand at 505.749 kb on + strand at 505.750 kb on - strand at 505.866 kb on + strand, within RR42_RS23050 at 505.867 kb on - strand, within RR42_RS23050 at 505.867 kb on - strand, within RR42_RS23050 at 505.867 kb on - strand, within RR42_RS23050 at 505.867 kb on - strand, within RR42_RS23050 at 505.867 kb on - strand, within RR42_RS23050 at 505.908 kb on + strand, within RR42_RS23050 at 506.023 kb on + strand, within RR42_RS23050 at 506.075 kb on + strand, within RR42_RS23050 at 506.076 kb on - strand, within RR42_RS23050 at 506.086 kb on + strand, within RR42_RS23050 at 506.221 kb on + strand, within RR42_RS23050 at 506.513 kb on + strand, within RR42_RS23050 at 506.514 kb on - strand, within RR42_RS23050
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 503,771 + -1.0 503,900 + +0.7 503,906 + +3.3 503,907 - +0.4 503,932 + -0.9 503,957 + -0.6 503,958 - +0.3 503,995 - +0.5 504,017 + +1.0 504,034 + +0.6 504,035 - +0.7 504,035 - +0.6 504,035 - +0.3 504,084 - -1.1 504,084 - +0.6 504,085 + -1.6 504,085 + -0.1 504,085 + +0.3 504,085 + -0.5 504,086 - +0.9 504,087 + -1.0 504,088 - +0.9 504,088 - +0.9 504,175 + -0.1 504,176 - -1.4 504,214 - -0.3 504,222 + -0.0 504,223 - -0.4 504,239 - +1.7 504,473 + RR42_RS23040 0.85 +0.7 504,478 + RR42_RS23040 0.87 +0.7 504,536 + +0.0 504,536 + +0.4 504,536 + +0.7 504,539 + -0.6 504,540 - +1.2 504,540 - +0.2 504,543 + +0.1 504,543 + +0.4 504,543 + +0.6 504,547 + +0.7 504,547 + -0.8 504,547 + -0.4 504,548 - +0.2 504,548 - -0.3 504,586 - +0.2 504,586 - -1.3 504,586 - +0.7 504,635 - -0.6 504,682 - -0.1 505,028 + RR42_RS23045 0.38 +0.0 505,028 + RR42_RS23045 0.38 -0.5 505,200 - RR42_RS23045 0.57 +0.3 505,222 - RR42_RS23045 0.60 +1.2 505,261 - RR42_RS23045 0.64 +0.9 505,261 - RR42_RS23045 0.64 -0.3 505,264 - RR42_RS23045 0.64 +0.3 505,264 - RR42_RS23045 0.64 -0.3 505,613 + -1.7 505,613 + +0.2 505,614 - +0.2 505,614 - +0.3 505,628 - -0.1 505,669 - +0.3 505,749 + -0.9 505,749 + +0.7 505,750 - -0.0 505,866 + RR42_RS23050 0.20 +0.5 505,867 - RR42_RS23050 0.20 -0.4 505,867 - RR42_RS23050 0.20 +0.7 505,867 - RR42_RS23050 0.20 +0.1 505,867 - RR42_RS23050 0.20 +0.3 505,867 - RR42_RS23050 0.20 +0.4 505,908 + RR42_RS23050 0.24 +0.3 506,023 + RR42_RS23050 0.35 +0.6 506,075 + RR42_RS23050 0.40 +0.6 506,076 - RR42_RS23050 0.40 +0.6 506,086 + RR42_RS23050 0.41 -2.7 506,221 + RR42_RS23050 0.53 -1.3 506,513 + RR42_RS23050 0.80 +0.6 506,514 - RR42_RS23050 0.80 +0.6
Or see this region's nucleotide sequence