Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21975 and RR42_RS21980 are separated by 59 nucleotides RR42_RS21980 and RR42_RS21985 are separated by 13 nucleotides
RR42_RS21975: RR42_RS21975 - hypothetical protein, at 258,302 to 262,522
_RS21975
RR42_RS21980: RR42_RS21980 - transporter, at 262,582 to 263,139
_RS21980
RR42_RS21985: RR42_RS21985 - sugar ABC transporter, at 263,153 to 264,598
_RS21985
Position (kb) 262
263
264 Strain fitness (log2 ratio) -2
-1
0
1 at 261.650 kb on + strand, within RR42_RS21975 at 261.651 kb on - strand, within RR42_RS21975 at 261.651 kb on - strand, within RR42_RS21975 at 261.659 kb on + strand, within RR42_RS21975 at 261.660 kb on - strand, within RR42_RS21975 at 261.696 kb on - strand, within RR42_RS21975 at 261.696 kb on - strand, within RR42_RS21975 at 262.097 kb on + strand, within RR42_RS21975 at 262.134 kb on - strand at 262.176 kb on - strand at 262.253 kb on + strand at 262.253 kb on + strand at 262.254 kb on - strand at 262.254 kb on - strand at 262.320 kb on - strand at 262.320 kb on - strand at 262.320 kb on - strand at 262.442 kb on + strand at 262.442 kb on + strand at 262.442 kb on + strand at 262.443 kb on - strand at 262.443 kb on - strand at 262.556 kb on + strand at 262.557 kb on - strand at 262.593 kb on + strand at 262.593 kb on + strand at 262.593 kb on + strand at 262.744 kb on + strand, within RR42_RS21980 at 262.744 kb on + strand, within RR42_RS21980 at 262.745 kb on - strand, within RR42_RS21980 at 262.961 kb on - strand, within RR42_RS21980 at 263.168 kb on - strand at 263.304 kb on - strand, within RR42_RS21985 at 263.978 kb on + strand, within RR42_RS21985 at 263.978 kb on + strand, within RR42_RS21985 at 264.066 kb on - strand, within RR42_RS21985 at 264.104 kb on + strand, within RR42_RS21985
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 261,650 + RR42_RS21975 0.79 -0.4 261,651 - RR42_RS21975 0.79 -0.4 261,651 - RR42_RS21975 0.79 +0.4 261,659 + RR42_RS21975 0.80 +0.6 261,660 - RR42_RS21975 0.80 +0.4 261,696 - RR42_RS21975 0.80 -0.1 261,696 - RR42_RS21975 0.80 +0.0 262,097 + RR42_RS21975 0.90 +0.2 262,134 - -1.1 262,176 - -0.3 262,253 + -0.2 262,253 + +1.2 262,254 - +0.2 262,254 - -1.0 262,320 - -1.0 262,320 - +0.5 262,320 - +0.4 262,442 + -0.1 262,442 + +0.2 262,442 + -0.6 262,443 - -0.0 262,443 - -0.8 262,556 + +0.1 262,557 - -0.2 262,593 + -0.2 262,593 + +0.2 262,593 + +0.1 262,744 + RR42_RS21980 0.29 +1.6 262,744 + RR42_RS21980 0.29 +0.0 262,745 - RR42_RS21980 0.29 +0.5 262,961 - RR42_RS21980 0.68 -1.3 263,168 - -0.2 263,304 - RR42_RS21985 0.10 +1.7 263,978 + RR42_RS21985 0.57 +0.4 263,978 + RR42_RS21985 0.57 -0.3 264,066 - RR42_RS21985 0.63 -1.9 264,104 + RR42_RS21985 0.66 +0.4
Or see this region's nucleotide sequence