Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16280 and RR42_RS16285 are separated by 193 nucleotides RR42_RS16285 and RR42_RS16290 are separated by 325 nucleotides
RR42_RS16280: RR42_RS16280 - transcriptional regulator, at 3,523,912 to 3,524,853
_RS16280
RR42_RS16285: RR42_RS16285 - methylase, at 3,525,047 to 3,526,198
_RS16285
RR42_RS16290: RR42_RS16290 - hypothetical protein, at 3,526,524 to 3,527,366
_RS16290
Position (kb)
3525
3526
3527 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3524.171 kb on - strand, within RR42_RS16280 at 3524.271 kb on + strand, within RR42_RS16280 at 3524.500 kb on + strand, within RR42_RS16280 at 3524.500 kb on + strand, within RR42_RS16280 at 3524.500 kb on + strand, within RR42_RS16280 at 3524.501 kb on - strand, within RR42_RS16280 at 3524.501 kb on - strand, within RR42_RS16280 at 3524.608 kb on + strand, within RR42_RS16280 at 3524.609 kb on - strand, within RR42_RS16280 at 3524.609 kb on - strand, within RR42_RS16280 at 3524.609 kb on - strand, within RR42_RS16280 at 3524.609 kb on - strand, within RR42_RS16280 at 3524.609 kb on - strand, within RR42_RS16280 at 3524.609 kb on - strand, within RR42_RS16280 at 3524.609 kb on - strand, within RR42_RS16280 at 3524.609 kb on - strand, within RR42_RS16280 at 3524.764 kb on + strand at 3524.766 kb on + strand at 3524.772 kb on + strand at 3524.773 kb on - strand at 3524.810 kb on + strand at 3524.810 kb on + strand at 3524.811 kb on - strand at 3524.843 kb on - strand at 3524.843 kb on - strand at 3524.882 kb on + strand at 3524.882 kb on + strand at 3524.883 kb on - strand at 3524.883 kb on - strand at 3524.992 kb on + strand at 3524.992 kb on + strand at 3524.993 kb on - strand at 3524.993 kb on - strand at 3525.046 kb on + strand at 3525.046 kb on + strand at 3525.047 kb on - strand at 3525.047 kb on - strand at 3525.047 kb on - strand at 3525.465 kb on - strand, within RR42_RS16285 at 3525.538 kb on + strand, within RR42_RS16285 at 3525.587 kb on + strand, within RR42_RS16285 at 3525.588 kb on - strand, within RR42_RS16285 at 3525.588 kb on - strand, within RR42_RS16285 at 3525.588 kb on - strand, within RR42_RS16285 at 3525.617 kb on + strand, within RR42_RS16285 at 3525.617 kb on + strand, within RR42_RS16285 at 3525.618 kb on - strand, within RR42_RS16285 at 3525.618 kb on - strand, within RR42_RS16285 at 3525.920 kb on + strand, within RR42_RS16285 at 3525.921 kb on - strand, within RR42_RS16285 at 3525.921 kb on - strand, within RR42_RS16285 at 3526.189 kb on + strand at 3526.196 kb on + strand at 3526.197 kb on - strand at 3526.197 kb on - strand at 3526.425 kb on + strand at 3526.425 kb on + strand at 3526.426 kb on - strand at 3526.426 kb on - strand at 3526.426 kb on - strand at 3526.426 kb on - strand at 3526.426 kb on - strand at 3526.431 kb on + strand at 3526.431 kb on + strand at 3526.431 kb on + strand at 3526.431 kb on + strand at 3526.431 kb on + strand at 3526.432 kb on - strand at 3526.432 kb on - strand at 3526.432 kb on - strand at 3526.432 kb on - strand at 3526.444 kb on + strand at 3526.496 kb on + strand at 3526.497 kb on - strand at 3526.557 kb on + strand at 3526.558 kb on - strand at 3526.586 kb on - strand at 3526.634 kb on + strand, within RR42_RS16290 at 3526.635 kb on - strand, within RR42_RS16290 at 3526.657 kb on - strand, within RR42_RS16290 at 3526.657 kb on - strand, within RR42_RS16290 at 3527.049 kb on + strand, within RR42_RS16290 at 3527.049 kb on + strand, within RR42_RS16290 at 3527.050 kb on - strand, within RR42_RS16290 at 3527.129 kb on - strand, within RR42_RS16290
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 3,524,171 - RR42_RS16280 0.27 -2.3 3,524,271 + RR42_RS16280 0.38 -1.8 3,524,500 + RR42_RS16280 0.62 -3.9 3,524,500 + RR42_RS16280 0.62 +0.2 3,524,500 + RR42_RS16280 0.62 -4.0 3,524,501 - RR42_RS16280 0.63 -0.4 3,524,501 - RR42_RS16280 0.63 -0.6 3,524,608 + RR42_RS16280 0.74 -5.1 3,524,609 - RR42_RS16280 0.74 -3.4 3,524,609 - RR42_RS16280 0.74 -1.7 3,524,609 - RR42_RS16280 0.74 -3.9 3,524,609 - RR42_RS16280 0.74 -3.3 3,524,609 - RR42_RS16280 0.74 -0.8 3,524,609 - RR42_RS16280 0.74 -2.6 3,524,609 - RR42_RS16280 0.74 -3.1 3,524,609 - RR42_RS16280 0.74 -0.9 3,524,764 + -1.9 3,524,766 + -4.3 3,524,772 + -3.8 3,524,773 - -3.5 3,524,810 + -0.9 3,524,810 + +0.0 3,524,811 - +1.2 3,524,843 - +0.4 3,524,843 - -0.0 3,524,882 + +0.8 3,524,882 + -0.8 3,524,883 - -0.5 3,524,883 - -1.2 3,524,992 + +0.1 3,524,992 + +0.1 3,524,993 - +0.0 3,524,993 - -0.7 3,525,046 + +0.7 3,525,046 + +0.5 3,525,047 - -0.2 3,525,047 - +0.0 3,525,047 - -0.0 3,525,465 - RR42_RS16285 0.36 +0.3 3,525,538 + RR42_RS16285 0.43 -0.9 3,525,587 + RR42_RS16285 0.47 +2.4 3,525,588 - RR42_RS16285 0.47 -0.3 3,525,588 - RR42_RS16285 0.47 +3.4 3,525,588 - RR42_RS16285 0.47 +0.7 3,525,617 + RR42_RS16285 0.49 +1.4 3,525,617 + RR42_RS16285 0.49 -1.0 3,525,618 - RR42_RS16285 0.50 +0.3 3,525,618 - RR42_RS16285 0.50 -0.3 3,525,920 + RR42_RS16285 0.76 +2.0 3,525,921 - RR42_RS16285 0.76 +1.2 3,525,921 - RR42_RS16285 0.76 +2.0 3,526,189 + +0.0 3,526,196 + -0.5 3,526,197 - +0.9 3,526,197 - -0.6 3,526,425 + +1.3 3,526,425 + +0.3 3,526,426 - -1.8 3,526,426 - -0.3 3,526,426 - +0.2 3,526,426 - +1.1 3,526,426 - +0.4 3,526,431 + +0.2 3,526,431 + +0.2 3,526,431 + +0.2 3,526,431 + -0.1 3,526,431 + -0.4 3,526,432 - +0.4 3,526,432 - -0.7 3,526,432 - -1.0 3,526,432 - -0.3 3,526,444 + -0.3 3,526,496 + -0.2 3,526,497 - -0.3 3,526,557 + +1.2 3,526,558 - +0.8 3,526,586 - -0.2 3,526,634 + RR42_RS16290 0.13 +0.4 3,526,635 - RR42_RS16290 0.13 +0.3 3,526,657 - RR42_RS16290 0.16 +0.0 3,526,657 - RR42_RS16290 0.16 -0.5 3,527,049 + RR42_RS16290 0.62 +0.8 3,527,049 + RR42_RS16290 0.62 -0.9 3,527,050 - RR42_RS16290 0.62 -0.2 3,527,129 - RR42_RS16290 0.72 +1.2
Or see this region's nucleotide sequence