Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20120

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20110 and BPHYT_RS20115 are separated by 25 nucleotidesBPHYT_RS20115 and BPHYT_RS20120 are separated by 182 nucleotidesBPHYT_RS20120 and BPHYT_RS20125 are separated by 396 nucleotides BPHYT_RS20110: BPHYT_RS20110 - sensor histidine kinase, at 54,486 to 55,868 _RS20110 BPHYT_RS20115: BPHYT_RS20115 - transcriptional regulator, at 55,894 to 56,592 _RS20115 BPHYT_RS20120: BPHYT_RS20120 - porin, at 56,775 to 57,899 _RS20120 BPHYT_RS20125: BPHYT_RS20125 - histidine kinase, at 58,296 to 58,511 _RS20125 Position (kb) 56 57 58Strain fitness (log2 ratio) -1 0 1at 55.877 kb on + strandat 56.026 kb on + strand, within BPHYT_RS20115at 56.469 kb on + strand, within BPHYT_RS20115at 56.475 kb on + strand, within BPHYT_RS20115at 56.625 kb on - strandat 56.930 kb on + strand, within BPHYT_RS20120at 57.318 kb on + strand, within BPHYT_RS20120at 57.528 kb on + strand, within BPHYT_RS20120at 57.808 kb on - strandat 57.974 kb on + strandat 57.975 kb on - strandat 58.135 kb on - strandat 58.696 kb on - strandat 58.897 kb on + strandat 58.898 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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55,877 + -0.8
56,026 + BPHYT_RS20115 0.19 +1.0
56,469 + BPHYT_RS20115 0.82 +1.3
56,475 + BPHYT_RS20115 0.83 +1.3
56,625 - +0.1
56,930 + BPHYT_RS20120 0.14 -0.0
57,318 + BPHYT_RS20120 0.48 -0.0
57,528 + BPHYT_RS20120 0.67 -1.6
57,808 - -0.4
57,974 + +1.1
57,975 - -0.2
58,135 - +0.1
58,696 - -1.4
58,897 + -0.2
58,898 - +1.5

Or see this region's nucleotide sequence