Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18960 and BPHYT_RS18965 are separated by 1 nucleotides BPHYT_RS18965 and BPHYT_RS18970 are separated by 86 nucleotides BPHYT_RS18970 and BPHYT_RS18975 are separated by 5 nucleotides BPHYT_RS18975 and BPHYT_RS18980 overlap by 4 nucleotides
BPHYT_RS18960: BPHYT_RS18960 - chemotaxis protein CheZ, at 4,259,168 to 4,259,878
_RS18960
BPHYT_RS18965: BPHYT_RS18965 - chemotaxis protein CheY, at 4,259,880 to 4,260,275
_RS18965
BPHYT_RS18970: BPHYT_RS18970 - chemotaxis protein, at 4,260,362 to 4,261,450
_RS18970
BPHYT_RS18975: BPHYT_RS18975 - chemotaxis protein CheD, at 4,261,456 to 4,262,271
_RS18975
BPHYT_RS18980: BPHYT_RS18980 - chemotaxis protein CheR, at 4,262,268 to 4,263,224
_RS18980
Position (kb) 4260
4261
4262 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 4259.388 kb on + strand, within BPHYT_RS18960 at 4259.389 kb on - strand, within BPHYT_RS18960 at 4259.389 kb on - strand, within BPHYT_RS18960 at 4259.688 kb on + strand, within BPHYT_RS18960 at 4259.689 kb on - strand, within BPHYT_RS18960 at 4259.963 kb on - strand, within BPHYT_RS18965 at 4260.128 kb on - strand, within BPHYT_RS18965 at 4260.131 kb on + strand at 4260.365 kb on + strand at 4260.366 kb on - strand at 4260.491 kb on - strand, within BPHYT_RS18970 at 4260.491 kb on - strand, within BPHYT_RS18970 at 4260.520 kb on - strand, within BPHYT_RS18970 at 4260.520 kb on - strand, within BPHYT_RS18970 at 4260.689 kb on - strand, within BPHYT_RS18970 at 4260.689 kb on - strand, within BPHYT_RS18970 at 4260.689 kb on - strand, within BPHYT_RS18970 at 4260.690 kb on + strand, within BPHYT_RS18970 at 4260.690 kb on + strand, within BPHYT_RS18970 at 4260.691 kb on - strand, within BPHYT_RS18970 at 4260.691 kb on - strand, within BPHYT_RS18970 at 4260.691 kb on - strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.741 kb on + strand, within BPHYT_RS18970 at 4260.742 kb on - strand, within BPHYT_RS18970 at 4260.742 kb on - strand, within BPHYT_RS18970 at 4260.742 kb on - strand, within BPHYT_RS18970 at 4260.742 kb on - strand, within BPHYT_RS18970 at 4260.742 kb on - strand, within BPHYT_RS18970 at 4260.784 kb on + strand, within BPHYT_RS18970 at 4260.791 kb on - strand, within BPHYT_RS18970 at 4260.847 kb on + strand, within BPHYT_RS18970 at 4260.847 kb on + strand, within BPHYT_RS18970 at 4260.847 kb on + strand, within BPHYT_RS18970 at 4260.848 kb on - strand, within BPHYT_RS18970 at 4260.848 kb on - strand, within BPHYT_RS18970 at 4260.848 kb on - strand, within BPHYT_RS18970 at 4261.096 kb on - strand, within BPHYT_RS18970 at 4261.903 kb on + strand, within BPHYT_RS18975 at 4261.903 kb on + strand, within BPHYT_RS18975 at 4261.904 kb on - strand, within BPHYT_RS18975 at 4262.018 kb on + strand, within BPHYT_RS18975 at 4262.183 kb on + strand, within BPHYT_RS18975 at 4262.237 kb on + strand at 4262.237 kb on + strand at 4262.238 kb on - strand at 4262.238 kb on - strand at 4262.271 kb on + strand at 4262.271 kb on + strand at 4262.272 kb on - strand at 4262.365 kb on + strand, within BPHYT_RS18980 at 4262.366 kb on - strand, within BPHYT_RS18980 at 4262.404 kb on + strand, within BPHYT_RS18980 at 4262.405 kb on - strand, within BPHYT_RS18980
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,259,388 + BPHYT_RS18960 0.31 -1.4 4,259,389 - BPHYT_RS18960 0.31 +0.3 4,259,389 - BPHYT_RS18960 0.31 -0.2 4,259,688 + BPHYT_RS18960 0.73 +1.0 4,259,689 - BPHYT_RS18960 0.73 -1.9 4,259,963 - BPHYT_RS18965 0.21 -3.1 4,260,128 - BPHYT_RS18965 0.63 -4.2 4,260,131 + -0.6 4,260,365 + -1.5 4,260,366 - -1.7 4,260,491 - BPHYT_RS18970 0.12 -1.7 4,260,491 - BPHYT_RS18970 0.12 -2.4 4,260,520 - BPHYT_RS18970 0.15 -5.5 4,260,520 - BPHYT_RS18970 0.15 -2.9 4,260,689 - BPHYT_RS18970 0.30 -3.2 4,260,689 - BPHYT_RS18970 0.30 -2.6 4,260,689 - BPHYT_RS18970 0.30 -3.2 4,260,690 + BPHYT_RS18970 0.30 -2.3 4,260,690 + BPHYT_RS18970 0.30 -1.6 4,260,691 - BPHYT_RS18970 0.30 -3.4 4,260,691 - BPHYT_RS18970 0.30 -2.3 4,260,691 - BPHYT_RS18970 0.30 -0.4 4,260,741 + BPHYT_RS18970 0.35 -2.0 4,260,741 + BPHYT_RS18970 0.35 -6.1 4,260,741 + BPHYT_RS18970 0.35 -3.2 4,260,741 + BPHYT_RS18970 0.35 -4.1 4,260,741 + BPHYT_RS18970 0.35 -1.6 4,260,741 + BPHYT_RS18970 0.35 -1.0 4,260,742 - BPHYT_RS18970 0.35 -0.7 4,260,742 - BPHYT_RS18970 0.35 -0.6 4,260,742 - BPHYT_RS18970 0.35 -1.8 4,260,742 - BPHYT_RS18970 0.35 -1.3 4,260,742 - BPHYT_RS18970 0.35 -3.2 4,260,784 + BPHYT_RS18970 0.39 -2.1 4,260,791 - BPHYT_RS18970 0.39 -3.6 4,260,847 + BPHYT_RS18970 0.45 -2.7 4,260,847 + BPHYT_RS18970 0.45 -5.6 4,260,847 + BPHYT_RS18970 0.45 -1.8 4,260,848 - BPHYT_RS18970 0.45 -1.9 4,260,848 - BPHYT_RS18970 0.45 -3.5 4,260,848 - BPHYT_RS18970 0.45 -3.9 4,261,096 - BPHYT_RS18970 0.67 -3.0 4,261,903 + BPHYT_RS18975 0.55 -2.2 4,261,903 + BPHYT_RS18975 0.55 -0.8 4,261,904 - BPHYT_RS18975 0.55 +0.2 4,262,018 + BPHYT_RS18975 0.69 -1.6 4,262,183 + BPHYT_RS18975 0.89 -1.8 4,262,237 + -0.7 4,262,237 + -3.5 4,262,238 - -1.4 4,262,238 - -1.3 4,262,271 + -1.3 4,262,271 + -0.8 4,262,272 - -2.5 4,262,365 + BPHYT_RS18980 0.10 -2.0 4,262,366 - BPHYT_RS18980 0.10 -1.5 4,262,404 + BPHYT_RS18980 0.14 -1.3 4,262,405 - BPHYT_RS18980 0.14 +0.4
Or see this region's nucleotide sequence