Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS18895

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS18885 and BPHYT_RS18890 are separated by 10 nucleotidesBPHYT_RS18890 and BPHYT_RS18895 are separated by 92 nucleotidesBPHYT_RS18895 and BPHYT_RS18900 are separated by 217 nucleotides BPHYT_RS18885: BPHYT_RS18885 - flagellar basal body rod modification protein FlgD, at 4,244,064 to 4,244,786 _RS18885 BPHYT_RS18890: BPHYT_RS18890 - flagellar basal body rod protein FlgC, at 4,244,797 to 4,245,222 _RS18890 BPHYT_RS18895: BPHYT_RS18895 - flagellar basal body rod protein FlgB, at 4,245,315 to 4,245,809 _RS18895 BPHYT_RS18900: BPHYT_RS18900 - flagella basal body P-ring formation protein FlgA, at 4,246,027 to 4,247,541 _RS18900 Position (kb) 4245 4246Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4244.547 kb on - strand, within BPHYT_RS18885at 4244.547 kb on - strand, within BPHYT_RS18885at 4244.726 kb on + strandat 4244.727 kb on - strandat 4244.933 kb on + strand, within BPHYT_RS18890at 4244.933 kb on + strand, within BPHYT_RS18890at 4244.934 kb on - strand, within BPHYT_RS18890at 4245.095 kb on + strand, within BPHYT_RS18890at 4245.096 kb on - strand, within BPHYT_RS18890at 4245.289 kb on - strandat 4245.316 kb on + strandat 4245.317 kb on - strandat 4245.317 kb on - strandat 4245.437 kb on + strand, within BPHYT_RS18895at 4245.437 kb on + strand, within BPHYT_RS18895at 4245.698 kb on - strand, within BPHYT_RS18895at 4245.698 kb on - strand, within BPHYT_RS18895at 4245.809 kb on + strandat 4245.809 kb on + strandat 4245.809 kb on + strandat 4245.809 kb on + strandat 4245.810 kb on - strandat 4245.810 kb on - strandat 4245.810 kb on - strandat 4245.810 kb on - strandat 4245.903 kb on + strandat 4245.903 kb on + strandat 4245.903 kb on + strandat 4245.935 kb on + strandat 4245.955 kb on + strandat 4245.955 kb on + strandat 4245.955 kb on + strandat 4245.955 kb on + strandat 4245.955 kb on + strandat 4245.955 kb on + strandat 4245.955 kb on + strandat 4246.290 kb on + strand, within BPHYT_RS18900at 4246.291 kb on - strand, within BPHYT_RS18900at 4246.291 kb on - strand, within BPHYT_RS18900at 4246.291 kb on - strand, within BPHYT_RS18900at 4246.291 kb on - strand, within BPHYT_RS18900at 4246.291 kb on - strand, within BPHYT_RS18900

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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4,244,547 - BPHYT_RS18885 0.67 -2.6
4,244,547 - BPHYT_RS18885 0.67 -2.9
4,244,726 + -1.1
4,244,727 - -3.6
4,244,933 + BPHYT_RS18890 0.32 -2.3
4,244,933 + BPHYT_RS18890 0.32 -1.6
4,244,934 - BPHYT_RS18890 0.32 -1.2
4,245,095 + BPHYT_RS18890 0.70 -3.4
4,245,096 - BPHYT_RS18890 0.70 -2.2
4,245,289 - -0.6
4,245,316 + -2.8
4,245,317 - -2.2
4,245,317 - -1.5
4,245,437 + BPHYT_RS18895 0.25 -4.1
4,245,437 + BPHYT_RS18895 0.25 +0.7
4,245,698 - BPHYT_RS18895 0.77 -2.9
4,245,698 - BPHYT_RS18895 0.77 -4.0
4,245,809 + -3.0
4,245,809 + -3.1
4,245,809 + -1.3
4,245,809 + -1.7
4,245,810 - -1.6
4,245,810 - -0.2
4,245,810 - -3.0
4,245,810 - -1.7
4,245,903 + -4.3
4,245,903 + -1.1
4,245,903 + -2.2
4,245,935 + -3.2
4,245,955 + -0.7
4,245,955 + -3.2
4,245,955 + -3.4
4,245,955 + +1.0
4,245,955 + -1.1
4,245,955 + -4.2
4,245,955 + -3.3
4,246,290 + BPHYT_RS18900 0.17 -3.4
4,246,291 - BPHYT_RS18900 0.17 -1.4
4,246,291 - BPHYT_RS18900 0.17 -3.2
4,246,291 - BPHYT_RS18900 0.17 -2.1
4,246,291 - BPHYT_RS18900 0.17 -3.0
4,246,291 - BPHYT_RS18900 0.17 -1.7

Or see this region's nucleotide sequence