Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18885 and BPHYT_RS18890 are separated by 10 nucleotides BPHYT_RS18890 and BPHYT_RS18895 are separated by 92 nucleotides BPHYT_RS18895 and BPHYT_RS18900 are separated by 217 nucleotides
BPHYT_RS18885: BPHYT_RS18885 - flagellar basal body rod modification protein FlgD, at 4,244,064 to 4,244,786
_RS18885
BPHYT_RS18890: BPHYT_RS18890 - flagellar basal body rod protein FlgC, at 4,244,797 to 4,245,222
_RS18890
BPHYT_RS18895: BPHYT_RS18895 - flagellar basal body rod protein FlgB, at 4,245,315 to 4,245,809
_RS18895
BPHYT_RS18900: BPHYT_RS18900 - flagella basal body P-ring formation protein FlgA, at 4,246,027 to 4,247,541
_RS18900
Position (kb)
4245
4246 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4244.547 kb on - strand, within BPHYT_RS18885 at 4244.547 kb on - strand, within BPHYT_RS18885 at 4244.726 kb on + strand at 4244.727 kb on - strand at 4244.933 kb on + strand, within BPHYT_RS18890 at 4244.933 kb on + strand, within BPHYT_RS18890 at 4244.934 kb on - strand, within BPHYT_RS18890 at 4245.095 kb on + strand, within BPHYT_RS18890 at 4245.096 kb on - strand, within BPHYT_RS18890 at 4245.289 kb on - strand at 4245.316 kb on + strand at 4245.317 kb on - strand at 4245.317 kb on - strand at 4245.437 kb on + strand, within BPHYT_RS18895 at 4245.437 kb on + strand, within BPHYT_RS18895 at 4245.698 kb on - strand, within BPHYT_RS18895 at 4245.698 kb on - strand, within BPHYT_RS18895 at 4245.809 kb on + strand at 4245.809 kb on + strand at 4245.809 kb on + strand at 4245.809 kb on + strand at 4245.810 kb on - strand at 4245.810 kb on - strand at 4245.810 kb on - strand at 4245.810 kb on - strand at 4245.903 kb on + strand at 4245.903 kb on + strand at 4245.903 kb on + strand at 4245.935 kb on + strand at 4245.955 kb on + strand at 4245.955 kb on + strand at 4245.955 kb on + strand at 4245.955 kb on + strand at 4245.955 kb on + strand at 4245.955 kb on + strand at 4245.955 kb on + strand at 4246.290 kb on + strand, within BPHYT_RS18900 at 4246.291 kb on - strand, within BPHYT_RS18900 at 4246.291 kb on - strand, within BPHYT_RS18900 at 4246.291 kb on - strand, within BPHYT_RS18900 at 4246.291 kb on - strand, within BPHYT_RS18900 at 4246.291 kb on - strand, within BPHYT_RS18900
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,244,547 - BPHYT_RS18885 0.67 -2.6 4,244,547 - BPHYT_RS18885 0.67 -2.9 4,244,726 + -1.1 4,244,727 - -3.6 4,244,933 + BPHYT_RS18890 0.32 -2.3 4,244,933 + BPHYT_RS18890 0.32 -1.6 4,244,934 - BPHYT_RS18890 0.32 -1.2 4,245,095 + BPHYT_RS18890 0.70 -3.4 4,245,096 - BPHYT_RS18890 0.70 -2.2 4,245,289 - -0.6 4,245,316 + -2.8 4,245,317 - -2.2 4,245,317 - -1.5 4,245,437 + BPHYT_RS18895 0.25 -4.1 4,245,437 + BPHYT_RS18895 0.25 +0.7 4,245,698 - BPHYT_RS18895 0.77 -2.9 4,245,698 - BPHYT_RS18895 0.77 -4.0 4,245,809 + -3.0 4,245,809 + -3.1 4,245,809 + -1.3 4,245,809 + -1.7 4,245,810 - -1.6 4,245,810 - -0.2 4,245,810 - -3.0 4,245,810 - -1.7 4,245,903 + -4.3 4,245,903 + -1.1 4,245,903 + -2.2 4,245,935 + -3.2 4,245,955 + -0.7 4,245,955 + -3.2 4,245,955 + -3.4 4,245,955 + +1.0 4,245,955 + -1.1 4,245,955 + -4.2 4,245,955 + -3.3 4,246,290 + BPHYT_RS18900 0.17 -3.4 4,246,291 - BPHYT_RS18900 0.17 -1.4 4,246,291 - BPHYT_RS18900 0.17 -3.2 4,246,291 - BPHYT_RS18900 0.17 -2.1 4,246,291 - BPHYT_RS18900 0.17 -3.0 4,246,291 - BPHYT_RS18900 0.17 -1.7
Or see this region's nucleotide sequence