Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS18865

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS18860 and BPHYT_RS18865 are separated by 2 nucleotidesBPHYT_RS18865 and BPHYT_RS18870 are separated by 36 nucleotidesBPHYT_RS18870 and BPHYT_RS18875 are separated by 12 nucleotides BPHYT_RS18860: BPHYT_RS18860 - flagellar P-ring protein FlgI, at 4,239,004 to 4,240,191 _RS18860 BPHYT_RS18865: BPHYT_RS18865 - flagellar L-ring protein FlgH, at 4,240,194 to 4,240,892 _RS18865 BPHYT_RS18870: BPHYT_RS18870 - flagellar basal body rod protein FlgG, at 4,240,929 to 4,241,717 _RS18870 BPHYT_RS18875: BPHYT_RS18875 - flagellar basal body rod protein FlgF, at 4,241,730 to 4,242,488 _RS18875 Position (kb) 4240 4241Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4239.244 kb on - strand, within BPHYT_RS18860at 4239.275 kb on - strand, within BPHYT_RS18860at 4239.518 kb on + strand, within BPHYT_RS18860at 4239.519 kb on - strand, within BPHYT_RS18860at 4239.698 kb on + strand, within BPHYT_RS18860at 4239.699 kb on - strand, within BPHYT_RS18860at 4239.699 kb on - strand, within BPHYT_RS18860at 4239.699 kb on - strand, within BPHYT_RS18860at 4240.080 kb on - strandat 4240.185 kb on + strandat 4240.186 kb on - strandat 4240.238 kb on + strandat 4240.256 kb on + strandat 4240.258 kb on + strandat 4240.259 kb on - strandat 4240.259 kb on - strandat 4240.259 kb on - strandat 4240.307 kb on - strand, within BPHYT_RS18865at 4240.307 kb on - strand, within BPHYT_RS18865at 4240.717 kb on + strand, within BPHYT_RS18865at 4240.875 kb on - strandat 4240.909 kb on + strandat 4240.930 kb on + strandat 4240.930 kb on + strandat 4240.930 kb on + strandat 4240.931 kb on - strandat 4241.000 kb on - strandat 4241.065 kb on + strand, within BPHYT_RS18870at 4241.065 kb on + strand, within BPHYT_RS18870at 4241.066 kb on - strand, within BPHYT_RS18870at 4241.378 kb on - strand, within BPHYT_RS18870at 4241.476 kb on + strand, within BPHYT_RS18870at 4241.477 kb on - strand, within BPHYT_RS18870at 4241.582 kb on - strand, within BPHYT_RS18870at 4241.701 kb on + strandat 4241.701 kb on + strandat 4241.701 kb on + strandat 4241.702 kb on - strandat 4241.735 kb on + strandat 4241.736 kb on - strandat 4241.736 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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4,239,244 - BPHYT_RS18860 0.20 -3.6
4,239,275 - BPHYT_RS18860 0.23 -3.2
4,239,518 + BPHYT_RS18860 0.43 -2.8
4,239,519 - BPHYT_RS18860 0.43 +0.0
4,239,698 + BPHYT_RS18860 0.58 -4.8
4,239,699 - BPHYT_RS18860 0.59 -2.4
4,239,699 - BPHYT_RS18860 0.59 +0.0
4,239,699 - BPHYT_RS18860 0.59 -2.4
4,240,080 - -3.5
4,240,185 + -3.7
4,240,186 - -3.4
4,240,238 + -3.7
4,240,256 + -4.5
4,240,258 + -2.8
4,240,259 - -2.3
4,240,259 - -0.9
4,240,259 - -1.6
4,240,307 - BPHYT_RS18865 0.16 -4.2
4,240,307 - BPHYT_RS18865 0.16 -2.9
4,240,717 + BPHYT_RS18865 0.75 -3.0
4,240,875 - -4.0
4,240,909 + -3.9
4,240,930 + -3.8
4,240,930 + -4.0
4,240,930 + -1.0
4,240,931 - -3.7
4,241,000 - -3.4
4,241,065 + BPHYT_RS18870 0.17 -4.0
4,241,065 + BPHYT_RS18870 0.17 -2.0
4,241,066 - BPHYT_RS18870 0.17 -2.9
4,241,378 - BPHYT_RS18870 0.57 -2.4
4,241,476 + BPHYT_RS18870 0.69 -0.8
4,241,477 - BPHYT_RS18870 0.69 -3.6
4,241,582 - BPHYT_RS18870 0.83 -4.3
4,241,701 + -2.2
4,241,701 + -3.4
4,241,701 + -2.8
4,241,702 - -0.6
4,241,735 + -1.7
4,241,736 - -1.8
4,241,736 - -4.7

Or see this region's nucleotide sequence