Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18830 and BPHYT_RS18835 are separated by 37 nucleotides BPHYT_RS18835 and BPHYT_RS18840 are separated by 97 nucleotides BPHYT_RS18840 and BPHYT_RS18845 are separated by 10 nucleotides
BPHYT_RS18830: BPHYT_RS18830 - flagellar biosynthesis protein FliQ, at 4,232,219 to 4,232,488
_RS18830
BPHYT_RS18835: BPHYT_RS18835 - flagellar biosynthesis protein FliR, at 4,232,526 to 4,233,308
_RS18835
BPHYT_RS18840: BPHYT_RS18840 - flagellar hook-associated protein FlgL, at 4,233,406 to 4,234,629
_RS18840
BPHYT_RS18845: BPHYT_RS18845 - flagellar hook-associated protein FlgK, at 4,234,640 to 4,236,613
_RS18845
Position (kb) 4233
4234
4235 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4232.911 kb on - strand, within BPHYT_RS18835 at 4233.060 kb on - strand, within BPHYT_RS18835 at 4233.234 kb on - strand at 4233.234 kb on - strand at 4233.269 kb on + strand at 4233.306 kb on + strand at 4233.395 kb on + strand at 4233.405 kb on - strand at 4233.792 kb on + strand, within BPHYT_RS18840 at 4233.928 kb on - strand, within BPHYT_RS18840 at 4233.929 kb on + strand, within BPHYT_RS18840 at 4233.929 kb on + strand, within BPHYT_RS18840 at 4233.929 kb on + strand, within BPHYT_RS18840 at 4233.930 kb on - strand, within BPHYT_RS18840 at 4233.930 kb on - strand, within BPHYT_RS18840 at 4233.930 kb on - strand, within BPHYT_RS18840 at 4233.960 kb on - strand, within BPHYT_RS18840 at 4233.960 kb on - strand, within BPHYT_RS18840 at 4233.960 kb on - strand, within BPHYT_RS18840 at 4234.194 kb on - strand, within BPHYT_RS18840 at 4234.229 kb on + strand, within BPHYT_RS18840 at 4234.356 kb on + strand, within BPHYT_RS18840 at 4234.356 kb on + strand, within BPHYT_RS18840 at 4234.356 kb on + strand, within BPHYT_RS18840 at 4234.377 kb on + strand, within BPHYT_RS18840 at 4234.377 kb on + strand, within BPHYT_RS18840 at 4234.446 kb on - strand, within BPHYT_RS18840 at 4234.551 kb on - strand at 4234.599 kb on + strand at 4234.599 kb on + strand at 4234.600 kb on - strand at 4234.600 kb on - strand at 4234.608 kb on - strand at 4234.722 kb on + strand at 4234.723 kb on - strand at 4234.852 kb on + strand, within BPHYT_RS18845 at 4234.863 kb on + strand, within BPHYT_RS18845 at 4234.863 kb on + strand, within BPHYT_RS18845 at 4235.091 kb on + strand, within BPHYT_RS18845 at 4235.091 kb on + strand, within BPHYT_RS18845 at 4235.092 kb on - strand, within BPHYT_RS18845 at 4235.124 kb on + strand, within BPHYT_RS18845 at 4235.124 kb on + strand, within BPHYT_RS18845 at 4235.125 kb on - strand, within BPHYT_RS18845 at 4235.197 kb on - strand, within BPHYT_RS18845 at 4235.496 kb on + strand, within BPHYT_RS18845 at 4235.497 kb on - strand, within BPHYT_RS18845 at 4235.497 kb on - strand, within BPHYT_RS18845 at 4235.511 kb on + strand, within BPHYT_RS18845 at 4235.512 kb on - strand, within BPHYT_RS18845 at 4235.512 kb on - strand, within BPHYT_RS18845 at 4235.601 kb on + strand, within BPHYT_RS18845
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,232,911 - BPHYT_RS18835 0.49 -1.3 4,233,060 - BPHYT_RS18835 0.68 -0.1 4,233,234 - +0.0 4,233,234 - -3.0 4,233,269 + -0.6 4,233,306 + -0.3 4,233,395 + +1.4 4,233,405 - +0.3 4,233,792 + BPHYT_RS18840 0.32 -4.7 4,233,928 - BPHYT_RS18840 0.43 -2.4 4,233,929 + BPHYT_RS18840 0.43 -2.0 4,233,929 + BPHYT_RS18840 0.43 -3.5 4,233,929 + BPHYT_RS18840 0.43 -2.6 4,233,930 - BPHYT_RS18840 0.43 -1.0 4,233,930 - BPHYT_RS18840 0.43 -2.6 4,233,930 - BPHYT_RS18840 0.43 -1.6 4,233,960 - BPHYT_RS18840 0.45 -2.1 4,233,960 - BPHYT_RS18840 0.45 -2.9 4,233,960 - BPHYT_RS18840 0.45 -4.1 4,234,194 - BPHYT_RS18840 0.64 -3.3 4,234,229 + BPHYT_RS18840 0.67 -2.4 4,234,356 + BPHYT_RS18840 0.78 -1.0 4,234,356 + BPHYT_RS18840 0.78 -1.9 4,234,356 + BPHYT_RS18840 0.78 -2.7 4,234,377 + BPHYT_RS18840 0.79 -1.8 4,234,377 + BPHYT_RS18840 0.79 -2.5 4,234,446 - BPHYT_RS18840 0.85 -3.2 4,234,551 - -2.6 4,234,599 + -3.9 4,234,599 + -5.0 4,234,600 - -2.7 4,234,600 - -2.4 4,234,608 - -1.0 4,234,722 + -2.5 4,234,723 - -4.5 4,234,852 + BPHYT_RS18845 0.11 -2.1 4,234,863 + BPHYT_RS18845 0.11 -3.8 4,234,863 + BPHYT_RS18845 0.11 -2.9 4,235,091 + BPHYT_RS18845 0.23 -2.4 4,235,091 + BPHYT_RS18845 0.23 -4.3 4,235,092 - BPHYT_RS18845 0.23 -1.4 4,235,124 + BPHYT_RS18845 0.25 -1.9 4,235,124 + BPHYT_RS18845 0.25 -2.8 4,235,125 - BPHYT_RS18845 0.25 -2.2 4,235,197 - BPHYT_RS18845 0.28 -2.3 4,235,496 + BPHYT_RS18845 0.43 -2.8 4,235,497 - BPHYT_RS18845 0.43 -4.9 4,235,497 - BPHYT_RS18845 0.43 -2.7 4,235,511 + BPHYT_RS18845 0.44 -5.4 4,235,512 - BPHYT_RS18845 0.44 -1.0 4,235,512 - BPHYT_RS18845 0.44 -4.0 4,235,601 + BPHYT_RS18845 0.49 -2.5
Or see this region's nucleotide sequence